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Contrary to common belief, effective communication is not only achieved via verbal exchanges of information. It requires a thoughtful combination of verbal and non-verbal cues since, in some circumstances, verbal communication in healthcare may be impossible, unfavourable or ineffective. The semiotics literature suggests that effective non-verbal communication goes beyond body language and gestures. It also includes the use of objects (i.e. traces) to transmit a message. We call this trace-based communication (TBC). In this perspective paper, we offer some reflections from our experience researching and using trace-based communication for training purposes. Our intention was to bring further awareness to the opportunities that trace-based communication might enable, including opportunities to enhance trainee-supervisor interactions, and consequently inform curriculum design.

The present commentary reviews the considerations of the famous American criminalist Paul Kirk in his seminal publication of 'The Ontogeny of Criminalistics,' written sixty years ago, regarding the status of forensic science and its fundamental principles. Professor Kirk aimed to examine forensic science as an independent scientific discipline, resulting in the identification of six key topics: 1) The need for fundamental principles; 2) the distinction between 'identification' and 'individualization;' 3) the qualifying elements of a profession; 4) the qualifying aspects of a science; 5) the need for a research-oriented basis; 6) the need for application of statistics and probability. In particular, Kirk deemed the nature of the progress made during his time as technical, practical, and transient at the cost of being fundamental, theoretical, and permanent. Predominantly, it is still the case today, with a post-effect fragmentation of forensic science into a myriad of ultra-specialized subdisciplines and applications. The lack of proper articulation of the most fundamental principles of forensic science was one of Kirk's most pressing concerns. The Sydney Declaration aimed to recapture the current fundamental (ontological and epistemological) status of forensic science, resulting in the redefinition of forensic science and its prime object of study, the trace, and in the consolidation of seven principles. The present commentary attempts to address Kirk's arguments in the Ontogeny in the context of the principles of the Declaration, with particular emphasis on the fundamental principles as well as Kirk's distinction between 'identification' and 'individualization,' which is considered critical to understanding the overall scope of forensic science., Competing Interests: Declaration of Competing Interest The author declares that he has no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

In this work, we present a compact LIBS sensor developed for characterization of samples on a crime scene following requirements of law enforcement agencies involved in the project. The sensor operates both in a tabletop mode, for aside measurements of swabbed materials or taken fragments, and in handheld mode where the sensor head is pointed directly on targets at the scene. The sensor head is connected via an umbilical to an instrument box that could be battery-powered and contains also a color camera for sample visualization, illumination LEDs, and pointing system for placing the target in focus. Here we describe the sensor's architecture and functionalities, the optimization of the acquisition parameters, and the results of some LIBS measurements. On nano-plotted traces at silica wafer and in optimized conditions, for most of the elements the detection limits, in term of the absolute element masses, were found to be below 10 picograms. We also show results obtained on some representative materials, like fingerprints, swabbed soil and gunshot residue, varnishes on metal, and coated plastics. The last, solid samples were used to evaluate the depth profiling capabilities of the instrument, where the recognition of all four car paint layers was achieved.

EBV+ diffuse large B cell lymphoma (DLBCL) not otherwise specified (NOS) is a new entity confirmed by the World Health Organization (WHO) in 2017. In this new entity, the virus may contribute to a tolerogenic microenvironment. Traces of the virus have been described in DLBCL with more sensitive methods, in cases that were originally diagnosed as negative. The aim of this study was to analyze the expression of immune response genes in the tumor microenvironment to disclose the role of the virus and its traces in DLBCL. In 48 DLBCL cases, the expression of immune response genes and the presence of molecules that induce tolerance, such as TIM3, LAG3 and PDL1 by immunohistochemistry (IHC), were studied. To broaden the study of the microenvironment, tumor-associated macrophages (TMAs) were also explored. No significant differences were observed in the expression of immune response genes in the EBV+ DLBCL and those cases that were EBV- DLBCL but that exhibited viral traces, assessed by ViewRNA assay. Only the EBV+ DLBCL cases displayed a significantly higher increase in the expression of CD8 and cytotoxic T cells detected by gene expression analysis, and of PDL1 in tumor cells and in the expression of CD68 in the tumor microenvironment detected by IHC, not observed in those cases with viral traces. The increase in CD8 and cytotoxic T cells, PDL1 and CD68 markers only in EBV+ DLBCL may indicate that traces of viral infection might not have influence in immune response markers., (© 2024. The Author(s).)

In 2017, the World Health Organization (WHO) confirmed a new entity, Epstein Barr virus (EBV) + Diffuse large B cell lymphoma (DLBCL), not otherwise specified (NOS). Traces of EBV transcripts were described in lymphomas, including DLBCL, that were diagnosed as EBV negative by conventional methods. The aim of this study was to detect viral genome by qPCR, as well as LMP1 and EBNA2 transcripts, with a more sensitive method in DLBCL cases from Argentina. Fourteen cases originally considered as EBV negative expressed LMP1 and/or EBNA2 transcripts. In addition, LMP1 and/or EBNA2 transcripts were also observed in bystander cells. However, EBERs+ cells cases by conventional ISH showed higher numbers of cells with LMP1 transcripts and LMP1 protein. In the cases that were EBERS- in tumor cells but with expression of LMP1 and/or EBNA2 transcripts, the viral load was below the limit of detection. This study provides further evidence that EBV could be detected in tumor cells by more sensitive methods. However, higher expression of the most important oncogenic protein, LMP1, as well as increased viral load, are only observed in cases with EBERs+ cells by conventional ISH, suggesting that traces of EBV might not display a key role in DLBCL pathogenesis., (© 2023 UICC.)

Physical and chemical properties have been studied in lithium niobate (LiNbO 3 , LN) crystals grown by Czochralski from a boron doped melt. Optical uniformity and optical damage resistance of LiNbO 3 :B crystals have been compared with control crystals of nominally pure congruent (CLN) and near-stoichiometric (NSLN K 2 O) composition. LiNbO 3 :B crystals structure has been studied. Studied LiNbO 3 :B crystals have been grown from differently synthesized charges. The charges have been synthesized from a mixture Nb 2 O 5 :B-Li 2 CO 3 using homogeneously doped Nb 2 O 5 :B precursor (sample 1, (B) = 0.0034 wt% in the charge) and by a direct solid phase synthesis from Nb 2 O 5 -Li 2 CO 3 -H 3 BO 3 mixture (sample 2, (B) = 0.0079 wt% in the charge). Only traces of boron (10 -5 -10 -4 wt%) have been detected in the samples. We have established that concentration of anti-site defects Nb Li is lower in both LiNbO 3 :B than in CLN crystals. XRD analysis has confirmed that B 3+ cations localize in faces of tetrahedral voids O 4 of LN structure. The voids act as buffers at the anion sublattice distortion. Sample 1 has been shown to have a structure closer to NSLN K 2 O crystal than sample 2. We have also shown that the chemical purity of LN crystal increases compared to the melt purity because boron creates strong compounds with impurities in the melt system Li 2 O-Nb 2 O 5 -B 2 O 3 . Metals impurities thus stay in the melt and do not transfer to the crystal.

Animal transport is debated due to welfare concerns. In this paper, data from 2014-2018 on animal transportation between EU member states (MS) (for slaughter, breeding, and production), are evaluated to create basis for discussion of TRACES database as tool to inform about pig and cattle welfare. Approximately 33million pigs were transported between EU MS peryear with considerable changes within categories.The majority of pigs was transported for production (57 to 73%), transportation for slaughter constituted 29% (34 to 25%), and the remaining (2-9%) were transported for breeding. Approximately 4 million cattle was transported peryear. The majority was transported for production (62 to 71%), 15-16% for slaughter, and the rest for breeding (23 to 14%). For each species and purpose category, 10 MS transporting the highest numbers are presented, suggesting acomplex underlying pattern with potential animal welfare consequences. Based on adiscussion of selected examples, however, we suggest that usefulness of TRACES as basis of an informed animal welfare evaluation would improve by including data from relevant animal types in addition to purpose categories.

The focus of the current study is to examine the collection and analysis of traces that are related to crime scene behaviors in sexual homicide cases as well as the factors influencing the solving of these crimes. Using 230 sexual homicide cases from the SHielD database, we computed two neural network models based on the multi-layer perceptron algorithm. First, we determined whether certain crime scene characteristics predicted the collection and analysis of traces (dependent variable for Model 1). Not surprisingly, the results indicate that trace collection and analysis were more likely to occur in sexual homicide cases with crime scene behaviors exhibiting the highest risk for trace transfer (e.g. close interactions with the victim) as well as the best conditions for trace persistence (e.g. body is found indoors). Situational and physical aspects of the crime scene are thus taken into account when deciding on the collection and analysis of traces. Second, we examined the situations in which the collection and analysis of traces contributes to crime solving (dependent variable for Model 2). The results suggest that the collection and analysis of traces does not necessarily predict the resolution of the case. Specifically, the analyses show that the collection and analysis of traces is useful for crime solving when: (1) the offenders' behaviors increase the opportunities for leaving traces at the crime scene, and (2) when the environmental and temporal aspects are favorable to the collection of traces. The impact of trace collection and analysis on case resolution is thus depending on the context of the case. Furthermore, the subsequent steps, such as the result of the trace analysis, the introduction into a database, the obtention of a result from this comparison, etc. might also affect case resolution, and thus interfere in the link between trace collection and analysis and case resolution., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

I study the physical nature of traces. Surprisingly, (i) systems separation with (ii) temperature differences and (iii) long thermalization times are sufficient conditions to produce macroscopic traces. Traces of the past are ubiquitous because these conditions are largely satisfied in our universe. I quantify these thermodynamical conditions for memory and derive an expression for the maximum amount of information stored in such memories as a function of the relevant thermodynamical parameters. This mechanism transforms low entropy into available information. I suggest that all macroscopic information has this origin in past low entropy.

The selective deposition of metals on dielectric materials is widely used in the electronic industry, making electro-conductive connections between circuit elements. We report a new low-cost laser-assisted method for the selective deposition of copper tracks on polymer surfaces by electroplating. The technique uses a laser for the selective modification of the polymer surface. The electrical conductivity of some polymers could be increased due to laser irradiation. Polyimide samples were treated using nanosecond and picosecond lasers working at a 1064 nm wavelength. An electro-conductive graphene-like layer was formed on the polymer surface after the laser treatment with selected parameters, and the copper layer thickness of 5-20 µm was deposited on the modified surface by electroplating. The selective laser-assisted electroplating technology allows the fabrication of copper tracks on complex shape dielectric materials. The technology could be used in the manufacturing of molded interconnect devices (MID).

Aim: To develop and test a scoring system for the technical quality of a urodynamic trace METHODS: Rasch analysis was used to select the most discriminating questions relating to good urodynamic practices. The questions were taken from International Continence Society standard documents and tested on 60 traces of varying quality and from different sources., Results: Twenty-five questions were selected by this process. These were tested with 10 traces by seven scorers, and with 60 traces by a single scorer. A pass quality score of 75% resulted in good discrimination between good and poor traces. Further editing and clarification resulted in a final score sheet of 23 Yes/No questions., Conclusion: A proposed scoring system, "Bristol UTraQ," for the technical quality of urodynamic traces has been developed and tested. We suggest a score above 75% indicates acceptable trace technical quality. High technical quality is the essential first step in ensuring that urodynamic studies can answer the urodynamic questions for every patient. Plans for further validation are outlined., (© 2022 Wiley Periodicals LLC.)

The EU is one of the main importers of marine ornamental species sourced from tropical coral reefs around the world. While the entrance of live organisms into the EU, along with their intra-EU circulation, is framed within stringent control mechanisms, to date, no reliable figures exist concerning which marine ornamental species are imported, in what numbers, and where they are sourced from. This lack of reliable data in the EU on the trade of marine ornamental species is puzzling if one considers that all these imported specimens must be controlled at customs offices located in international airports. Such data deficiency favors the prevalence of blurry supply chains and a "business as usual" mindset that hampers any serious effort to promote sustainability in the marine aquarium industry. To safeguard the collection of findable, accessible, interoperable, and reusable (FAIR) data, we suggest that the EU platform Trade Control and Expert System (TRACES) refines its surveillance on the trade of marine ornamental species. The detailed survey of marine ornamental fishes alone can be used as a proof of concept to validate the use of TRACES for this purpose and, if successful, it can later be expanded to all other taxonomic groups of marine ornamental species.

Forensic Advisors at the National Institute for Criminalistics and Criminology in Brussels act as advising body to the magistrate regarding analytical possibilities and the usefulness of trace analysis in a case. Initially, their function was devised to assist in complex murder cases with unknown offender. However, in a previous study, the increasing diversity of the cases they are requested for has been observed (Bitzer et al., in press). In order to deepen our understanding of the decision steps in the criminal investigation process, the decision to involve a Forensic Advisor and the factors leading to their involvement were evaluated. The study focused on homicide, robbery and burglary cases with and without requests for a Forensic Advisor between January 2014 and June 2016. The factors were categorised into five knowledge dimensions: strategic, immediate, physical, criminal and utility. Decision tree modelling was carried out in order to identify the factors influencing the request for a Forensic Advisor in the case. The decision to request a Forensic Advisor differs between different types of offences. It also depends on the complexity of the case in terms of the number of traces and objects collected at the crime scene, and the availability of witness reports. Indeed, Forensic Advisors take the role of trace analysis coordinator by providing an overview of all available traces, objects, analyses and results. According to the principal implication factors and the performed case study, the contribution of Forensic Advisors consists mainly in summarising all information and advise on potential additional analyses. This might be explained by a loss of overview of the information and the possibilities regarding trace analysis by the magistrate responsible of the case., (Copyright © 2018 Elsevier B.V. All rights reserved.)

The approaches for increasing a contact-free sampling distance up to 40 cm for a field asymmetric ion mobility spectrometer were investigated and implemented by use both the vortex flow made by a rotating impeller and the laser desorption of traces of low volatile explosives. The sampling device for a laser-based field asymmetric ion mobility spectrometer including a high-speed rotating impeller was designed and built with help of computer simulation of vortex and analytical flows. The dependence of a signal of trinitrotoluene vapors on a rotational speed of an impeller was obtained. The optimization of analytical flow was performed. The effective sampling distance is increased up to 28 cm for trinitrotoulene vapors detection by a field asymmetric ion mobility spectrometer equipped with a rotating impeller. The distance can be increased up to 40 cm using laser irradiation of objects with traces of explosives. It was shown that under ambient conditions the efficient desorption of low-volatile explosives is achieved at laser intensity 10 7  W/cm 2 , wavelength λ = 266 nm, pulse energy about 1 mJ and pulse frequency not less than 10 Hz.

This article aims at presenting the first results of a transdisciplinary research programme in heritage sciences. Based on the growing use and on the potentialities of micro- and nano-characterization synchrotron-based methods to study ancient materials (archaeology, palaeontology, cultural heritage, past environments), this contribution will identify and test conceptual and methodological elements of convergence between physicochemical and historical sciences.

This article presents a global vision of images in forensic science. The proliferation of perspectives on the use of images throughout criminal investigations and the increasing demand for research on this topic seem to demand a forensic science-based analysis. In this study, the definitions of and concepts related to material traces are revisited and applied to images, and a structured approach is used to persuade the scientific community to extend and improve the use of images as traces in criminal investigations. Current research efforts focus on technical issues and evidence assessment. This article provides a sound foundation for rationalising and explaining the processes involved in the production of clues from trace images. For example, the mechanisms through which these visual traces become clues of presence or action are described. An extensive literature review of forensic image analysis emphasises the existing guidelines and knowledge available for answering investigative questions (who, what, where, when and how). However, complementary developments are still necessary to demystify many aspects of image analysis in forensic science, including how to review and select images or use them to reconstruct an event or assist intelligence efforts. The hypothetico-deductive reasoning pathway used to discover unknown elements of an event or crime can also help scientists understand the underlying processes involved in their decision making. An analysis of a single image in an investigative or probative context is used to demonstrate the highly informative potential of images as traces and/or clues. Research efforts should be directed toward formalising the extraction and combination of clues from images. An appropriate methodology is key to expanding the use of images in forensic science., (Copyright © 2014 Forensic Science Society. Published by Elsevier Ireland Ltd. All rights reserved.)

Beebread is among the matrices suspected of contaminating honeybee. To better understand this contamination, the study aimed to develop an efficient, sensitive and reliable analytical method for the trace analysis of pesticides in beebread. This study focuses specifically on the insecticides pyrethroids and neonicotinoids and some of their metabolites. It describes the development and validation of an original analytical approach that consists of one simple extraction method based on modified QuEChERS followed by a selective and sensitive analysis by UHPLC-MS/MS to determine the target compounds in beebread. The method was validated using a quadratic fit. RSD values below 20% were obtained, except for 5-hydroxy-imidacloprid and imidacloprid at 0.5 ng/g, which exhibited RSDs of 25% and 21%, respectively. The intra-day precision was less than 10% for many of the investigated compounds. The inter-day precision varied between 2% and 36%, depending on the compound and the concentration. The recoveries varied from 53% to 119%, with averages of 83, 81 and 77% for the extraction of beebread samples spiked at 0.5, 5 and 10 ng/g, respectively. The LOD values for all the substances were below ng/g, with the exception of 6-chloronicotinic acid (LOD=1.7 ng/g). The method was then applied to the analysis of 32 beebread samples and revealed the presence of 7 of the target substances. The most frequently detected pesticides belonged to the neonicotinoid family and were generally present at low concentrations, but in some cases exceeded 170 ng/g (acetamiprid and thiacloprid). Some pyrethroids were also detected (lambda-cyhalothrine and bifenthrine), but at very low levels., (Copyright © 2013 Elsevier B.V. All rights reserved.)