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Hydroponic produce is gaining popularity due to its suitability for urban agriculture. The general public also considers that hydroponic produce is free from microbiological contamination. In this study, we compared the frequency and abundance of tetracycline-resistant and sulphadiazine-resistant bacteria and the minimal inhibitory concentration (MIC) of these isolates in conventional, organic, and hydroponic lettuce sold in retail. We also determined the frequency of samples carrying tetB , tetX , sul1 , sul2 , and int1 genes by PCR and further quantified the copy number of tetX , sul1 , and int1 genes in samples positive for these genes using qPCR. As expected, the number of resistant bacteria and the MICs of these isolates were lowest in hydroponic lettuce and highest in organic lettuce. All tested resistant genes, except int1 , were detected in samples of all three production methods, but no significant difference was observed between the three groups in the frequency of samples carrying the resistance genes examined or in their copy number. To the best of our knowledge, it is the first study directly reporting the existence of antibiotic-resistant bacteria and resistance genes in hydroponic vegetables sold in retail. The result highlights that the risk of antibiotic-resistant bacteria contamination in hydroponic produce should be further investigated.

In addition to environmental pollution issues, social concerns about the sustainability, safety, and quality of conventionally grown fruits and vegetables have been increasing. In order to evaluate if there were any microbiological differences between samples of organic and conventional lettuce, a wide range of parameters were tested, including pathogens and indicator organisms: the enumeration of Escherichia coli ; the detection of Salmonella spp.; the detection/enumeration of Listeria monocytogenes ; the enumeration of lactic acid bacteria, Pseudomonas spp. yeasts and molds, and Enterobacteriaceae . This study also evaluated the chemical safety of the lettuce samples, quantifying the nitrate concentration and 20 pesticides (14 organochlorine and 6 organophosphorus pesticides). Significant differences ( p < 0.05) between the conventional and organic samples were only detected for the counts of total microorganisms at 30 °C. Pathogens were absent in all the samples. The analytical method, using the quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach for pesticide extraction, was suitable for detecting the targeted analytes; the limit of quantification (LOQ) was between 0.6 and 1.8 µg/kg (lower than the Maximum Residue Levels (MRLs) established by EU legislation). In three organic lettuce samples, one organochlorine pesticide (α-HCH) was observed below the MRLs. For the samples analyzed and for the parameters investigated, except for the total mesophilic counts, the organic and conventional lettuces were not different.

Germination and sprouting are regulated by the energy status. In the present study, mung bean seeds were treated with adenosine triphosphate and 2,4-dinitrophenol (DNP). The metabolomic changes during development of mung beans under different energy statuses were investigated. In total, 42 metabolites were identified. Principal component analysis revealed that the featured compounds produced in seeds were oleic, linoleic, and succinic acids. Sugars, including maltose, sucrose, and glucose were related to sprouting. Mung bean seeds utilised diverse energy resources and produced higher succinic acid content. Sugars and secondary metabolites accumulated in sprouts. Nitrogen, sugar, and amino acid metabolism pathways contributed to this physiological process. DNP caused an energy deficit, which resulted in the consumption and translation of glucose. Higher contents of other saccharides and amino acids were observed. The transcriptional results further confirmed our metabolic hypothesis. In conclusion, sufficient energy supply is crucial for sprout development and nutritive metabolite synthesis., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The sanitising effects of combined lactic acid (LA, 2%, v/v) and low-concentration sodium hypochlorite (SH, 4 mg/L) on Listeria innocua were investigated. The disinfectant kinetics and percentage of injured cells showed that the combined treatment resulted in dramatic inactivation of L. innocua after 60 s. The Weibull model with a high R 2 (1.00) and low root mean squared error (RMSE) (0.19) and Akaike Information Criterion (AIC) (-7.58) was verified as the most superior model to describe effects of the combination treatment. Protein leakage and the fluorescence intensity ratio revealed that LA might disrupt the cytoplasmic membrane rapidly and that SH could synergistically promote the sanitising effect. Atomic force microscopy (AFM) images showed notable morphological changes in the combination treated cells, which presented lower width (0.73 μm) and height (0.29 μm), and increased roughness (10.37 nm). Moreover, massive leakage of intracellular components indicated severe cell collapse. The antimicrobial effects on organic broccoli sprouts showed that the combined treatment resulted in reductions of 0.82, 1.51, and 1.77 log colony forming units (CFU)/g fresh weight (FW) for aerobic bacteria, yeasts and moulds, and inoculated L. innocua at day 0. In addition, there were no significant differences of sensory quality results between treatment and control groups and they were all greater than the acceptance limit. Thus, the disinfection treatments had no negative effects on the storage quality of the organic broccoli sprouts. The combined treatment could be a potential sanitising strategy for organic sprouts., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Fresh produce is usually eaten raw without cooking or heating, which may increase the probability of foodborne infection. The microbiological quality of 11 types of fresh, raw vegetables (romaine lettuce, sesame leaves, crown daisy, garlic chives, iceberg lettuce, cabbage, broccoli, leek, chili pepper, capsicum, and zucchini) purchased at retail markets in Iksan, Korea as affected by cultivation method (environmentally friendly vegetables [organic, pesticide-free, and low-pesticide vegetables] and conventionally grown vegetables) and harvest season was determined. Escherichia coli O157:H7 and Salmonella were not detected in all samples of vegetables tested. Aerobic mesophiles (>6 log cfu/g) were detected in environmentally friendly romaine lettuce and crown daisy and environmentally friendly and conventionally grown garlic chives, which also contained coliforms (>3 log cfu/g). Sesame leaf and crown daisy (regardless of cultivation method), as well as conventionally grown romaine lettuce and leek, contained >1 log cfu/g of E. coli. The overall microbiological quality of environmentally friendly and conventionally grown vegetables was not significantly different (P > 0.05). However, there were seasonal effects on populations of coliforms and generic E. coli on vegetables. The greatest numbers of microorganisms were isolated from environmentally friendly or conventionally grown vegetables purchased in winter. The vegetables, regardless of cultivation method or season, should be subjected to appropriate antimicrobial treatment to enhance their microbial safety., (© 2014 Institute of Food Technologists®)

Aquaponics is an effective method to practice sustainable agriculture and is gaining popularity in the US; however, the microbial safety of aquaponically grown produce needs to be ascertained. Aquaponics is a unique marriage of fish production and soil-free produce (e.g., leafy greens) production. Fish are raised in fresh water tanks that are connected to water filled beds where fruits and vegetables are grown. The fish bi-products create nutrient-rich water that provides the key elements for the growth of plants and vegetables. The objective of this study was to perform a comparative analysis of the microbial safety and quality of aquaponic lettuce and soil grown lettuce (conventional, bagged, certified organic, and field lettuce). Following this, an intervention study was performed to combat foodborne pathogen surrogates ( Salmonella and E. coli ), spoilage, and fecal microorganisms using 2.5% acetic acid. The results of the comparative analysis study showed that aquaponically grown lettuce had significantly lower concentration of spoilage and fecal microorganisms compared to in-soil grown lettuce. The intervention study showed that diluted vinegar (2.5% acetic acid) significantly reduced Salmonella , E. coli , coliforms, and spoilage microorganisms on fresh lettuce by 2 to 3 log CFU/g. Irrespective of growing methods (in-soil or soilless), it is crucial to incorporate good agricultural practices to reduce microbial contamination on fresh produce. The intervention employed in this study can be proposed to small farmers and consumers to improve quality and safety of leafy greens.