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As the Internet-of-Things is deployed widely, many time-series data are generated everyday. Thus, classifying time-series automatically has become important. Compression-based pattern recognition has attracted attention, because it can analyze various data universally with few model parameters. RPCD (Recurrent Plots Compression Distance) is known as a compression-based time-series classification method. First, RPCD transforms time-series data into an image called "Recurrent Plots (RP)". Then, the distance between two time-series data is determined as the dissimilarity between their RPs. Here, the dissimilarity between two images is computed from the file size, when an MPEG-1 encoder compresses the video, which serializes the two images in order. In this paper, by analyzing the RPCD, we give an important insight that the quality parameter for the MPEG-1 encoding that controls the resolution of compressed videos influences the classification performance very much. We also show that the optimal parameter value depends extremely on the dataset to be classified: Interestingly, the optimal value for one dataset can make the RPCD fall behind a naive random classifier for another dataset. Supported by these insights, we propose an improved version of RPCD named qRPCD, which searches the optimal parameter value by means of cross-validation. Experimentally, qRPCD works superiorly to the original RPCD by about 4% in terms of classification accuracy.

Gamma delta (GD) T cells are an unconventional T cell type present in both the epidermis and the dermis of human skin. They are critical to regulating skin inflammation, wound healing, and anti-microbial defense. Similar to CD8+ cytotoxic T cells expressing an alpha beta (AB) TCR, GD T cells have cytolytic capabilities. They play an important role in elimination of cutaneous tumors and virally infected cells and have also been implicated in pathogenicity of several autoimmune diseases. T cell cytotoxicity is associated with the expression of the pore forming protein Perforin. Perforin is an innate immune protein containing a membrane attack complex perforin-like (MACPF) domain and functions by forming pores in the membranes of target cells, which allow granzymes and reactive oxygen species to enter the cells and destroy them. Perforin-2, encoded by the gene MPEG1 , is a newly discovered member of this protein family that is critical for clearance of intracellular bacteria. Cutaneous GD T cells express both Perforin and Perforin-2, but many questions remain regarding the role that these proteins play in GD T cell mediated cytotoxicity against tumors and bacterial pathogens. Here, we review what is known about Perforin expression by skin GD T cells and the mechanisms that contribute to Perforin activation., (Copyright © 2020 O'Neill, Pastar, Tomic-Canic and Strbo.)

The invertebrate innate immune system is surprisingly complex, yet our knowledge is limited to a few select model systems. One understudied group is the phylum Cnidaria (corals, sea anemones, etc.). Cnidarians are the sister group to Bilateria and by studying their innate immunity repertoire, a better understanding of the ancestral state can be gained. Corals in particular have evolved a highly diverse innate immune system that can uncover evolutionarily basal functions of conserved genes and proteins. One rudimentary function of the innate immune system is defense against harmful bacteria using pore forming proteins. Macrophage expressed gene 1/Perforin-2 protein (Mpeg-1/P2) is a particularly important pore forming molecule as demonstrated by previous studies in humans and mice, and limited studies in non-bilaterians. However, in cnidarians, little is known about Mpeg-1/P2. In this perspective article, we will summarize the current state of knowledge of Mpeg-1/P2 in invertebrates, analyze identified Mpeg-1/P2 homologs in cnidarians, and demonstrate the evolutionary diversity of this gene family using phylogenetic analysis. We will also show that Mpeg-1 is upregulated in one species of stony coral in response to lipopolysaccharides and downregulated in another species of stony coral in response to white band disease. This data presents evidence that Mpeg-1/P2 is conserved in cnidarians and we hypothesize that it plays an important role in cnidarian innate immunity. We propose that future research focus on the function of Mpeg-1/P2 family in cnidarians to identify its primary role in innate immunity and beyond., (Copyright © 2020 Walters, Connelly, Young and Traylor-Knowles.)