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Background: The prognostic analysis of lung invasive mucinous adenocarcinoma (IMA) is deficient due to the lack of a universally recommended histological grading system, leading to unregulated treatment approaches., Objective: We aimed to examine the clinical trajectory of IMA and assess the viability of utilizing the existing grading system for lung invasive non-mucinous adenocarcinoma in the context of IMA., Methods: We retrospectively collected clinicopathological data from 265 IMA patients. Each case re-evaluated the tumor grade using the following three classification systems: the 4th Edition of the World Health Organization classification system, the International Association for the Study of Lung Cancer (IASLC) grading system, and a two-tier grading system. We performed a comparative analysis of these grading systems and identified the most effective grading system for IMA., Results: The study comprised a total of 214 patients with pure IMA and 51 patients with mixed IMA. The 5-year overall survival (OS) rates for pure IMA and mixed IMA were 86.7% and 57.8%, respectively. All three grading systems proved to be effective prognostic classifiers for IMA. The value of area under the curve at 1-, 3-, and 5-year OS was highest for the IASLC grading system compared with the other grade systems and the clinical stage. The IASLC classification system was an independent prognostic predictor (p = 0.009, hazard ratio 2.243, 95% confidence interval 1.219-4.127)., Conclusion: Mixed IMA is more aggressive than pure IMA, with an OS rate on par with that of high-grade pure IMA. The IASLC grading system can better indicate prognosis and is recommended for lung IMA., (© 2024. Society of Surgical Oncology.)

Gram-negative bacteria use the T6SS to eject effectors into prey cells, aided by delivery domains. Recently uncovered by Carobbi et al., a new delivery domain, PIX, allowed the identification of hundreds of new effectors. They are order- and function-specific and exclusively orphan effectors, raising novel questions in the field., Competing Interests: Declaration of interests The author declares no conflict of interests., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Objectives: The overall goal of the survey was to ascertain from belters common characteristics of belting and belters as determined by their own experience. The survey sought information from those who responded to an online questionnaire stating they were professional singers who had been paid to sing as belters and were comfortable using the sound quality called belting. The survey asked various questions regarding the definition of the word belting, how the singers learned to belt, whether or not they could explain the auditory and kinesthetic experience of belting and their history with vocal health. This was a partial follow-up to a study done by one of the authors in 2007 on the same topic and with the same general population which was never submitted for publication. The data from that study, which was longer and more complex, will be referenced where the present survey has similar data, partly to see if answers may have changed in the time since data was collected more than a decade ago., Methods: The survey was an online questionnaire on Google. A call to respond to the survey was put forth by the authors through their network of colleagues, associates and students with the request that respondents also share the survey with others they knew. The questionnaire consisted of 30 questions and garnered a total of 238 responses from professional singers who consider themselves to be belters. Data was collected between February 1, 2019 and July 31, 2019 Topics included Demographics, Vocal Training Background, Definitions, and Vocal Health., Results/conclusion: The data collected presents demographics on vocal training, professional experience, gender, age, performance venues, definitions of key words, and vocal health issues. The majority of respondents are female, from the USA, have had training, and sing other styles of music in a belt quality. Specific statistics on each question are given. The earlier study, from 2007, referenced several similar questions from a smaller group of respondents. Corresponding information will be presented where applicable at the end of the article for purposes of comparison., (Copyright © 2021 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

Widespread human activity has resulted in the presence of different pollutants in the aquatic environment that does not exist in isolation. The study of the effects of contamination of aquatic organisms is of great significance. To assess the individual and combined toxicity of cadmium (Cd) and tributyltin (TBT) to aquatic organisms, juvenile grass carp (Ctenopharyngodon idella) were exposed to Cd (2.97 mg/L), TBT (7.5 μg/L), and their mixture MIX. The biological response was evaluated by nuclear magnetic resonance (NMR) analysis of plasma metabolites. Plasma samples at 1, 2, 4, 8, 16, 32, and 48 days post-exposure were analyzed using detection by NMR technique. The typical correlation analysis (CCA) analysis revealed that TBT had the greatest effect on plasma metabolism, followed by MIX and Cd. The interference pathway to grass carp was similar to that of TBT and MIX. Both Cd and TBT exposure alone or in combination can lead to metabolic abnormalities in TCA cycle-related pathways and interfere with energy metabolism. These results provide more detailed information for the metabolic study of pollutants and data for assessing the health risks of Cd, TBT, and MIX at the metabolic level., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Background: Patch test (PT) reactions to thiuram mix (TM) and fragrance mix (FM) I or II without concomitant reactions to their single constituents are potentially caused by the irritant properties of the mixes., Objective: Comparing inflammatory profiles of PT reactions to TM, FM I, FM II, and their constituents and assessing their potential in discrimination of irritant and allergic reactions., Patients and Methods: Levels of 14 cytokines and natural moisturizing factor (NMF) were determined in stratum corneum samples collected from PT reactions to TM, FM I or II, their constituents, and petrolatum (pet.) control sites in 36 individuals., Results: Levels of interleukin (IL)-16, chemokine (CXC motif) ligand (CXCL) 8, CXCL10, chemokine (CC motif) ligand (CCL) 17, and CCL22 were significantly increased in reactions (+, ++) to thiurams and fragrances compared to their petrolatum. controls, except for PT reactions to FM I/II with negative breakdown testing in which, however, decreased levels of NMF were observed. In doubtful reactions to FM I/II with negative breakdown testing, NMF was significantly lower than in petrolatum controls., Conclusions: PT reactions to thiurams and fragrances indicate a Th2-skewed inflammation. The inflammatory profiles suggest that weak or doubtful FM I/II reactions without accompanying reaction to a constituent were irritant. IL-16 might be suitable to distinguish irritant from allergic reaction., (© 2020 The Authors. Contact Dermatitis published by John Wiley & Sons Ltd.)

Experimental asymmetries in fusion implosions can lead to magnetic field generation in the hot plasma core. For typical parameters, previous studies found that the magnetization Hall parameter, given by the product of the electron gyro-frequency and Coulomb collision time, can exceed one. This will affect the hydrodynamics through inhibition and deflection of the electron heat flux. The magnetic field source is the collisionless Biermann term, which arises from the Debye shielding potential in electron pressure gradients. We show that there is an additional source term due to the Z dependence of the Coulomb collision operator. If there are ion composition gradients, such as jets of carbon ablator mix entering the hot-spot, this source term can rapidly exceed the Biermann fields. In addition, the Biermann fields are enhanced due to the increased temperature gradients from carbon radiative cooling. With even stronger self-generated fields, heat loss to the carbon regions will be reduced, potentially reducing the negative effect of carbon mix. This article is part of a discussion meeting issue 'Prospects for high gain inertial fusion energy (part 1)'.

The arrival of a baby, in all cultures, is a special moment for the parents, which brings with it numerous changes. The specificity of mixed couples magnifies these changes and requires certain elements to be negotiated in order to help the new family blend together. A clinical story shows how to envisage a creative way of constituting a mixed family., (Copyright © 2020 Elsevier Masson SAS. All rights reserved.)

Nursing is a highly feminised profession, yet includes a certain proportion of men. What this does fairly low mix mean, and what are its effects on the way teams work and on career paths?, (Copyright © 2020 Elsevier Masson SAS. All rights reserved.)

Background: Methylchloroisothiazolinone/methylisothiazolinone (MCI/MI) and methylisothiazolinone (MI) are tested to detect contact allergy to these isothiazolinones., Objectives: To study if an aqueous patch test preparation with MCI and MI in a mix of 0.015% and 0.2%, respectively, detects more contact allergies than the commonly used preparations of MCI/MI in 0.02% aq. and MI in 0.2% aq., Methods: A total of 1555 patients with dermatitis in five Swedish dermatology departments were tested consecutively with MCI/MI 0.215% aq., MCI/MI 0.02% aq., and MI 0.2% aq., Results: The share of contact allergy to MCI/MI 0.215% aq., MCI/MI 0.02% aq., and MI 0.2% aq. varied in the test centers between 7.9% and 25.9%, 3.2% and 10.3%, and 5.8% and 12.3%, respectively. MCI/MI 0.215% aq. detected significantly more patch-test positive individuals than both MCI/MI 0.02% aq. (P < .001) and MI 0.2% aq. (P < .001), as well as either one of MCI/MI and MI (P < .001). In the patients only reacting to MCI/MI 0.215% aq., 57.7% were recorded as having a dermatitis that was explained or aggravated by exposure to either MCI/MI or MI., Conclusion: The results speak in favor of replacing the preparations MCI/MI 0.02% aq. and MI 0.2% aq. with MCI/MI 0.215% aq. as the screening substance in the Swedish baseline series, which has been implemented in 2020., (© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Telomere shortening is involved in age-related disorders, such as cancer and cardiovascular diseases. Recently, telomerase re-activation strategies have been proposed to counteract telomere shortening and its consequences. Here, we investigated the benefit of dietary supplementation with a mix of S-adenosyl-methionine (SAMe) and a polysaccharide extract of Astragalus (APS) on telomere length of circulating lymphocytes of healthy volunteers. Blood lymphocytes of a cohort of 26 healthy volunteers who were administrated the mix of SAMe and APS in a food supplement for one year were collected. In vitro treatment of blood lymphocytes of healthy volunteers with the mix was also performed. A cohort of 150 healthy volunteers was used as a control. Telomere length was measured by Q-FISH. The micronucleus assay was performed to detect genotoxicity of the mix. The telomeres of circulating lymphocytes of the cohort of 26 donors supplemented with the mix were significantly longer than those of matched controls (p &lt; 10-4). This elongation was essentially observed in the lymphocytes of older donors. Similarly, in vitro treatment of circulating lymphocytes with the mix significantly increased telomere length and decrease the proportion of cells with short telomeres. Here, we observed an increase in telomere length after in vivo and in vitro administration of a mix with SAMe and APS.  The benefit of dietary supplementation with this mix opens a new horizon for the battle against aging and could be used in the treatment of chronic age-related disorders.

The cumulative adsorption of external phosphorus (P) by water purification sludge after ignition under different addition methods (mix and cover) was investigated along with the adsorption isotherm and the release of internal P after external P adsorption. The cumulative adsorption of external P was 11.496 mg (mix) and 11.042 mg (cover) and these values were higher than those in the control (7.219 mg). The maximum sorption capacity ( S ) increased under the mix and cover and the former (7.795 mg·g max ) increased under the mix and cover and the former (7.795 mg·g -1 ) was higher than the latter (6.807 mg·g -1 ) in the mix was 0.83 mg·L 0 ) in the mix was 0.83 mg·L -1 ). The result suggests that the internal P was easily released in the mix method, compared to the cover method. Under anaerobic conditions, the release of the internal P was 0.93 mg in the mix, lower than that in the cover (1.49 mg) and in the control (7.76 mg). In addition, the specific release rate in the cover method was 0.00614 (mean), higher than that (0.00396) in the mix method. Noticeably, these data were lower than those in the control, indicating that the retention of P under the mix method was higher, compared to the cover and the control and it is challenging to evaluate the P retention with EPC -1 ). The result suggests that the internal P was easily released in the mix method, compared to the cover method. Under anaerobic conditions, the release of the internal P was 0.93 mg in the mix, lower than that in the cover (1.49 mg) and in the control (7.76 mg). In addition, the specific release rate in the cover method was 0.00614 (mean), higher than that (0.00396) in the mix method. Noticeably, these data were lower than those in the control, indicating that the retention of P under the mix method was higher, compared to the cover and the control and it is challenging to evaluate the P retention with EPC 0 .

The influence of mountain front recharge on the water balance of alluvial valley aquifers located in upland catchments of the Condamine River basin in Queensland, Australia, is investigated through the development of an integrated hydrogeological framework. A combination of three-dimensional (3D) geological modelling, hydraulic gradient maps, multivariate statistical analyses and hydrochemical mixing calculations is proposed for the identification of hydrochemical end-members and quantification of the relative contributions of each end-member to alluvial aquifer recharge. The recognised end-members correspond to diffuse recharge and lateral groundwater inflows from three hydrostratigraphic units directly connected to the alluvial aquifer. This approach allows mapping zones of potential inter-aquifer connectivity and areas of groundwater mixing between underlying units and the alluvium. Mixing calculations using samples collected under baseflow conditions reveal that lateral contribution from a regional volcanic aquifer system represents the majority (41%) of inflows to the alluvial aquifer. Diffuse recharge contribution (35%) and inflow from two sedimentary bedrock hydrostratigraphic units (collectively 24%) comprise the remainder of major recharge sources. A detailed geochemical assessment of alluvial groundwater evolution along a selected flowpath of a representative subcatchment of the Condamine River basin confirms mixing as a key process responsible for observed spatial variations in hydrochemistry. Dissolution of basalt-related minerals and dolomite, CO 2 uptake, ion-exchange, precipitation of clay minerals, and evapotranspiration further contribute to the hydrochemical evolution of groundwater in the upland alluvial aquifer. This study highlights the benefits of undertaking an integrated approach that combines multiple independent lines of evidence. The proposed methods can be applied to investigate processes associated with inter-aquifer mixing, including groundwater contamination resulting from depressurisation of underlying geological units hydraulically connected to the shallower water reservoirs., (Copyright © 2016 Elsevier B.V. All rights reserved.)

Objectives/hypothesis: To gather qualitative descriptions of music theater vocal qualities including belt, legit, and mix from expert pedagogues to better define this voice type., Study Design: This is a prospective, semistructured interview., Methods: Twelve expert teachers from United States, United Kingdom, Asia, and Australia were interviewed by Skype and asked to identify characteristics of music theater vocal qualities including vocal production, physiology, esthetics, pitch range, and pedagogical techniques. Responses were compared with published studies on music theater voice., Results: Belt and legit were generally described as distinct sounds with differing physiological and technical requirements. Teachers were concerned that belt should be taught "safely" to minimize vocal health risks. There was consensus between teachers and published research on the physiology of the glottis and vocal tract; however, teachers were not in agreement about breathing techniques. Neither were teachers in agreement about the meaning of "mix." Most participants described belt as heavily weighted, thick folds, thyroarytenoid-dominant, or chest register; however, there was no consensus on an appropriate term. Belt substyles were named and generally categorized by weightedness or tone color. Descriptions of male belt were less clear than for female belt., Conclusions: This survey provides an overview of expert pedagogical perspectives on the characteristics of belt, legit, and mix qualities in the music theater voice. Although teacher responses are generally in agreement with published research, there are still many controversial issues and gaps in knowledge and understanding of this vocal technique. Breathing techniques, vocal range, mix, male belt, and vocal registers require continuing investigation so that we can learn more about efficient and healthy vocal function in music theater singing., (Copyright © 2016 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

Estrogenic chemicals are able to alter mammary gland development in female rodents, but little is known on the effects of anti-androgens and mixtures of endocrine disrupting chemicals (EDCs) with dissimilar modes of action. Pregnant rat dams were exposed during gestation and lactation to mixtures of environmentally relevant EDCs with estrogenic, anti-androgenic or dissimilar modes of action (TotalMix) of 100-, 200- or 450-fold high end human intake estimates. Mammary glands of prepubertal and adult female and male offspring were examined. Oestrogens increased mammary outgrowth in prepubertal females and the mRNA level of matrix metalloproteinase-3, which may be a potential biomarker for increased outgrowth. Mixtures of EDCs gave rise to ductal hyperplasia in adult males. Adult female mammary glands of the TotalMix group showed morphological changes possibly reflecting increased prolactin levels. In conclusion both estrogenic and anti-androgenic chemicals given during foetal life and lactation affected mammary glands in the offspring., (Copyright © 2014 Elsevier Inc. All rights reserved.)

In the present study, a 79-year-old male was referred to Kobe City Medical Center General Hospital due to an abnormal shadow that was revealed on a chest X-ray. The patient possessed a five-year history of consolidation in the right lower lobe, which was diagnosed as chronic aspiration pneumonia and followed up. However, the abnormal shadow adjacent to the pleura gradually increased in size and a novel mass appeared in the right lower lobe that rapidly increased in size. A repeat biopsy revealed a combination of large cell neuroendocrine carcinoma with a clinical tumor-node-metastasis (cTNM) stage of cT2bN2M0 (stage 3A) and mucosa-associated lymphoid tissue lymphoma at Ann Arbor stage 1E. Chemoradiotherapy markedly affected the lesion and the size of the mass was significantly reduced subsequent to four cycles of chemotherapy, which was considered to be a near complete response. The present study reports an extremely rare combination of tumors. The disease course was followed over a period of six years, which included the onset of disease, and the present case may therefore be valuable in clarifying the mechanism of lung cancer development.

This paper considers whether the extent of inequality in house prices within neighbourhoods of England is associated with depressive symptoms in the older population using the English Longitudinal Study of Ageing. We consider two competing hypotheses: first, the wealth inequality hypothesis which proposes that neighbourhood inequality is harmful to health and, second, the mixed neighbourhood hypothesis which suggests that socially mixed neighbourhoods are beneficial for health outcomes. Our results are supportive of the mixed neighbourhood hypothesis, we find a significant association between neighbourhood inequality and depression with lower levels of depression amongst older people in neighbourhoods with greater house price inequality after controlling for individual socio-economic and area correlates of depression. The association between area inequality and depression is strongest for the poorest individuals, but also holds among the most affluent. Our results are in line with research that suggests there are social and health benefits associated with economically mixed communities., (Copyright © 2014 Elsevier Ltd. All rights reserved.)

This study presents a methodology using an end-member mixing analysis (EMMA) and MIX to compute mixing ratios and to identify hydrochemical reactions in groundwater. The methodology consists of (1) identifying the potential sources of recharge, (2) characterising recharge sources and mixed water samples using hydrogeochemistry, (3) selecting chemical species to be used in the analysis and (4) calculating mixing ratios and identification of hydrochemical reactions in groundwater. This approach has been applied in the Besòs River Delta area, where we have collected 51 groundwater samples and a long data register of the hydrogeochemistry of the Besòs River created by the Catalan Water Agency is also available. The EMMA performed in the Besòs River suggests that 3 end-members are required to explain its temporal variability, accounting for the species chloride, sulphate, sodium, bicarbonate, calcium, magnesium, potassium, ammonium, total nitrogen, and electrical conductivity. One river end-member is from the wet periods (W1), and two are from dry periods (D1 and D2). These end-members have been used to compute mixing ratios in groundwater samples because the Besòs River is considered the main recharge source for the aquifer. Overall, dry season end-members dominated over the wet season end-member, in a proportion of 4:1. Moreover, when departures from the mixing line exist, geochemical processes might be identified. Redox processes, carbonate dissolution/precipitation and ion exchange processes may occur in Besòs Delta aquifer., (Copyright © 2013 Elsevier B.V. All rights reserved.)

We previously investigated the progression of β-amyloid deposition in brain of mice over-expressing amyloid-precursor protein (APP-Swe), a model of Alzheimer's disease (AD), in a longitudinal PET study with the novel β-amyloid tracer [(18)F]-florbetaben. There were certain discrepancies between PET and autoradiographic findings, which seemed to arise from partial volume effects (PVE). Since this phenomenon can lead to bias, most especially in the quantitation of brain microPET studies of mice, we aimed in the present study to investigate the magnitude of PVE on [(18)F]-florbetaben quantitation in murine brain, and to establish and validate a useful correction method (PVEC). Phantom studies with solutions of known radioactivity concentration were performed to measure the full-width-at-half-maximum (FWHM) resolution of the Siemens Inveon DPET and to validate a volume-of-interest (VOI)-based PVEC algorithm. Several VOI-brain-masks were applied to perform in vivo PVEC on [(18)F]-florbetaben data from C57BL/6(N=6) mice, while uncorrected and PVE-corrected data were cross-validated with gamma counting and autoradiography. Next, PVEC was performed on longitudinal PET data set consisting of 43 PET scans in APP-Swe (13-20months) and age-matched wild-type (WT) mice using the previously defined masks. VOI-based cortex-to-cerebellum ratios (SUVR) were compared for uncorrected and PVE-corrected results. Brains from a subset of transgenic mice were ultimately examined by autoradiography ex vivo and histochemistry in vitro as gold standard assessments, and compared to VOI-based PET results. The phantom study indicated a FWHM of 1.72mm. Applying a VOI-brain-mask including extracerebral regions gave robust PVEC, with increased precision of the SUVR results. Cortical SUVR increased with age in APP-Swe mice compared to baseline measurements (16months: +5.5%, p<0.005; 20months: +15.5%, p<0.05) with uncorrected data, and to a substantially greater extent with PVEC (16months: +12.2% p<0.005; 20months: +36.4% p<0.05). WT animals showed no binding changes, irrespective of PVEC. Relative to autoradiographic results, the error [%] for uncorrected cortical SUVR was 18.9% for native PET data, and declined to 4.8% upon PVEC, in high correlation with histochemistry results. We calculate that PVEC increases by 10% statistical power for detecting altered [(18)F]-florbetaben uptake in aging APP-Swe mice in planned studies of disease modifying treatments on amyloidogenesis., (© 2013.)

Lipoprotein lipase (LPL) and angiopoietin-like protein 4 (Angptl4) were studied in 3T3-L1 adipocytes. Transfections of the adipocytes with Angptl4 esiRNA caused reduction of the expression of Angptl4 to about one fourth of that in cells treated with vehicle only. This resulted in higher levels of LPL activity both on cell surfaces (heparin-releasable) and in the medium, while LPL activity within the cells remained unaffected. This demonstrated that even though both proteins are made in the same cell, Angptl4 does not inactivate LPL during intracellular transport. Most of the Angptl4 protein was present as covalent dimers and tetramers on cell surfaces, while within the cells there were only monomers. LPL gradually lost activity when incubated in medium, but there was no marked difference between conditioned medium from normal cells (rich in Angptl4) and medium after knockdown of Angptl4. Hence Angptl4 did not markedly accelerate inactivation of LPL in the medium. Experiments with combinations of different cells and media indicated that inactivation of LPL occurred on the surfaces of cells producing Angptl4., (Copyright © 2013 Elsevier Inc. All rights reserved.)