Objective: To evaluate the efficacy of daily cleaning with 4% chlorhexidine-impregnated sponges in decreasing contamination of blood cultures in critically-ill patients., Material and Methods: Prospective, quasi-experimental, longitudinal, single-centre trial. During 24 months (April 2013 to March 2015), we analysed 237 patients who fulfilled the inclusion criteria, divided into 2groups: one underwent daily cleaning with common soap (control group, n= 108), and the other with chlorhexidine (intervention group, n= 109). Demographic variables, pathology group, severity scores, ICU and hospital length of stay and mortality, and time passed since cleaning to blood culture extraction were included., Results: Statistical analysis showed a higher proportion of contaminated blood cultures during the control group period in contrast with the intervention group period (15 vs. 6.3%), with a significant difference: 9.23% (CI95%: 1,34-16,7%), with an odds ratio of 2,73 (CI95%: 1,13-6,63). Surveillance analysis showed lower probability of blood culture contamination within the 18hours following daily cleaning. Cleaning without chlorhexidine increased contamination of blood cultures (HR: 3,05; CI95%: 1,14-8,12)., Conclusions: The use of 4% chlorhexidine-impregnated sponges for daily cleaning of critically-ill patients decreases blood culture contamination incidence and its protection lasts for almost 18h., (Copyright © 2016 Sociedad Española de Enfermería Intensiva y Unidades Coronarias (SEEIUC). Publicado por Elsevier España, S.L.U. All rights reserved.)