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Background/Objectives : Obstetric ultrasound is one of the most commonly used imaging modalities during pregnancy to detect any fetal abnormalities. The aim of this systematic review was to appraise all available scientific literature and summarize current evidence regarding the safety of fetal ultrasound by using the thermal index (TI) and mechanical index (MI). Methods : We applied the PRISMA guidelines in order to prepare the review, and a 2-step process was performed in order to evaluate the available literature and decide which studies to be included. A thorough search of the Medline, Scopus, and Google Scholar databases was performed. Randomized and non-randomized studies were considered for review. The MI and TI were available in ultrasound machines after 1993; thus, studies before that year would not provide data on these two indexes. Results: A total of 21 studies were included in this review, including prospective, retrospective, cross-sectional, and survey-type studies. A common theme of the majority of the studies is the increased acoustic output available to the machines with time and the limited awareness of where the MI/TI indexes are from the operators. Conclusions: This review indicates that, while obstetric ultrasound is predominantly safe, there is a need for operators to consistently observe MI/TI indexes and adhere to the ALARA principle to minimize potential risks.

Study Objectives: From 2019-2023, the United States Food and Drug Administration has cleared 9 novel obstructive sleep apnea-detecting wearables for home sleep apnea testing, with many now commercially available for sleep clinicians to integrate into their clinical practices. To help clinicians comprehend these devices and their functionalities, we meticulously reviewed their operating mechanisms, sensors, algorithms, data output, and related performance evaluation literature., Methods: We collected information from PubMed, United States Food and Drug Administration clearance documents, ClinicalTrials.gov, and web sources, with direct industry input whenever feasible., Results: In this "device-centered" review, we broadly categorized these wearables into 2 main groups: those that primarily harness photoplethysmography data and those that do not. The former include the peripheral arterial tonometry-based devices. The latter was further broken down into 2 key subgroups: acoustic-based and respiratory effort-based devices. We provided a performance evaluation literature review and objectively compared device-derived metrics and specifications pertinent to sleep clinicians. Detailed demographics of study populations, exclusion criteria, and pivotal statistical analyses of the key validation studies are summarized., Conclusions: In the foreseeable future, these novel obstructive sleep apnea-detecting wearables may emerge as primary diagnostic tools for patients at risk for moderate-to-severe obstructive sleep apnea without significant comorbidities. While more devices are anticipated to join this category, there remains a critical need for cross-device comparison studies as well as independent performance evaluation and outcome research in diverse populations. Now is the moment for sleep clinicians to immerse themselves in understanding these emerging tools to ensure our patient-centered care is improved through the appropriate implementation and utilization of these novel sleep technologies., Citation: Chiang AA, Jerkins E, Holfinger S, et al. OSA diagnosis goes wearable: are the latest devices ready to shine? J Clin Sleep Med . 2024;20(11):1823-1838., (© 2024 American Academy of Sleep Medicine.)

Stored products, such as grains and processed foods, are susceptible to infestation by various insects. The early detection of insects in the supply chain is crucial, as introducing invasive pests to new environments may cause disproportionate harm. The STAR Center at Stevens Institute of Technology developed the Acoustic Stored Product Insect Detection System (A-SPIDS) to detect pests in stored products. The system, which comprises a sound-insulated container for product samples with a built-in internal array of piezoelectric sensors and additional electret microphones to record outside noise, was used to conduct numerous measurements of the vibroacoustic signatures of various insects, including the Callosobruchus maculatus , Tribolium confusum , and Tenebrio molitor , in different materials. A normalization method was implemented using the ambient noise of the sensors as a reference, to accommodate for the proprietary, non-calibrated sensors and allowing to set relative detection thresholds for unknown sensitivities. The normalized envelope of the filtered signals was used to characterize and compare the insect signals by estimating the Normalized Signal Pulse Amplitude (NSPA) and the Normalized Spectral Energy Level (NSEL). These parameters characterize the insect detection Signal Noise Ratio (SNR) for pulse-based detection (NSPA) and averaged energy-based detection (NSEL). These metrics provided an initial step towards the design of a reliable detection algorithm. In the conducted tests NSPA was significantly larger than NSEL. The NSPA reached 70 dB for T. molitor in corn flakes. The insect signals were lower in flour where the averaged NSPA and NSEL values were around 40 dB and 11 dB to 16 dB, respectively.

Speech impairment is a common and disabling symptom in Parkinson's disease (PD), affecting communication and quality of life. Advances in digital speech processing and artificial intelligence have revolutionized objective speech analysis. Given the complex nature of speech impairment, acoustic speech analysis offers unique biomarkers for neuroprotective treatments from the prodromal stages of PD. Digital speech biomarkers can monitor levodopa-induced motor complications, detect the effects of deep brain stimulation, and provide feedback for behavioral speech therapy. This review updates the mechanisms underlying speech impairment, the impact of speech phenotypes, and the effects of interventions on speech. We evaluate the strengths, potential weaknesses, and suitability of promising digital speech biomarkers in PD for capturing disease progression and treatment efficacy. Additionally, we explore the translational potential of PD speech biomarkers to other neuropsychiatric diseases, offering insights into motion, cognition, and emotion. Finally, we highlight knowledge gaps and suggest directions for future research to enhance the use of quantitative speech measures in disease-modifying clinical trials. The findings demonstrate that one year is sufficient to detect disease progression in early PD through speech biomarkers. Voice quality, pitch, loudness, and articulation measures appear to capture the efficacy of treatment interventions most effectively. Certain speech features, such as loudness and articulation rate, behave oppositely in different neurological diseases, offering valuable insights for differential diagnosis. In conclusion, this review highlights speech as a biomarker in tracking disease progression, especially in the prodromal stages of PD, and calls for further longitudinal studies to establish its efficacy across diverse populations., Competing Interests: Declaration of Competing Interest All authors declare that they have no conflicts of interest., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Advances in additive manufacturing in composites have transformed aerospace, medical devices, tissue engineering, and electronics. A key aspect of enhancing properties of 3D-printed objects involves fine-tuning the material by embedding and orienting reinforcement within the structure. Existing methods for orienting these reinforcements are limited by pattern types, alignment, and particle characteristics. Acoustics offers a versatile method to control the particles independent of their size, geometry, and charge, enabling intricate pattern formations. However, integrating acoustics into 3D printing has been challenging due to the scattering of the acoustic field between polymerized layers and unpolymerized resin, resulting in unwanted patterns. To address this challenge, SonoPrint, an innovative acoustically assisted volumetric 3D printer is developed that enables simultaneous reinforcement patterning and printing of the entire structure. SonoPrint generates mechanically tunable composite geometries by embedding reinforcement particles, such as microscopic glass, metal, and polystyrene, within the fabricated structure. This printer employs a standing wave field to create targeted particle motifs-including parallel lines, radial lines, circles, rhombuses, hexagons, and polygons-directly in the photosensitive resin, completing the print in just a few minutes. SonoPrint enhances structural properties and promises to advance volumetric printing, unlocking applications in tissue engineering, biohybrid robots, and composite fabrication., (© 2024 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.)

Purpose: The aim of this pilot study was to explore the efficacy of an intensive 3 week water resistance phonation (WRP) therapy program for people with presbyphonia. Method: Participants included 13 people with presbyphonia who received intensive WRP therapy. All participants completed eight sessions of therapy over 3 weeks. Auditory perceptual ratings, and acoustic and aerodynamic assessments were performed before and after treatment. Participants also completed the voice-related quality of life questionnaire before and after the treatment. Result: After 3 weeks of intensive voice therapy, significant improvements were demonstrated in acoustic, aerodynamic, and auditory perceptual parameters, as well as patient perceptions of voice-related quality of life. Acoustically, it was found there were significant decreases in shimmer ( p  = 0.019), noise-to-harmonic ratio ( p  = 0.016), and smoothed cepstral peak prominence ( p  = 0.001). Perceptually, the clients with presbyphonia showed significant reductions in the ratings of the overall grade, roughness, asthenia, and strain. Moreover, there was a significant increase in the total score of the Mandarin version of the Voice-Related Quality of Life measure post-therapy. Conclusion: The investigation provides preliminary evidence that people with presbyphonia can improve their vocal function and voice-related quality of life through intensive WRP therapy within a short period of time.

Background: About one-third of older adults aged 65 years and older often have mild cognitive impairment or dementia. Acoustic and psycho-linguistic features derived from conversation may be of great diagnostic value because speech involves verbal memory and cognitive and neuromuscular processes. The relative decline in these processes, however, may not be linear and remains understudied., Objective: This study aims to establish associations between cognitive abilities and various attributes of speech and natural language production. To date, the majority of research has been cross-sectional, relying mostly on data from structured interactions and restricted to textual versus acoustic analyses., Methods: In a sample of 71 older (mean age 83.3, SD 7.0 years) community-dwelling adults who completed qualitative interviews and cognitive testing, we investigated the performance of both acoustic and psycholinguistic features associated with cognitive deficits contemporaneously and at a 1-2 years follow up (mean follow-up time 512.3, SD 84.5 days)., Results: Combined acoustic and psycholinguistic features achieved high performance (F 1 -scores 0.73-0.86) and sensitivity (up to 0.90) in estimating cognitive deficits across multiple domains. Performance remained high when acoustic and psycholinguistic features were used to predict follow-up cognitive performance. The psycholinguistic features that were most successful at classifying high cognitive impairment reflected vocabulary richness, the quantity of speech produced, and the fragmentation of speech, whereas the analogous top-ranked acoustic features reflected breathing and nonverbal vocalizations such as giggles or laughter., Conclusions: These results suggest that both acoustic and psycholinguistic features extracted from qualitative interviews may be reliable markers of cognitive deficits in late life., (©Varsha D Badal, Jenna M Reinen, Elizabeth W Twamley, Ellen E Lee, Robert P Fellows, Erhan Bilal, Colin A Depp. Originally published in JMIR Aging (https://aging.jmir.org), 16.09.2024.)

Objective: Audiological tests on smartphones require consistent microphone recordings across device types with a reasonable standard uncertainty (2-3 Decibel (dB)) of the sound pressure level at the microphone. However, the calibration of smartphone microphones by the non-expert user is still an unsolved issue. We show that whistling on standardized glass bottles permits a coarse sound level calibration with an uncertainty that is smaller than the standard uncertainty of clinical audiograms (4.9dB) and enough for mobile health (mHealth) products., Design: We define and test a calibration procedure with bottle-whistles for smartphones. The empirical sound pressure levels are used to calculate the mean and standard deviation of a single measurement., Study Sample: Two uncalibrated studies with a total of 30 participants, one calibrated study with 11 participants., Results: The mean maximal sound pressure level of 330 ml Vichy-shape bottle-whistles at 50 cm distance is 92.8 ± 1.6dB sound pressure level (SPL). The sound pressure level variation of a single measurement is 3.0dB SPL., Conclusions: In comparison to other possible ways of level calibration estimates for smartphones (e.g. level of own voice, level of common environmental sounds), the current method appears to be robust in background noise and easily reproducible with glass bottles of defined dimensions.

Sound symbolism, the idea that the sound of a word alone can convey its meaning, is often studied using auditory pseudowords. For example, people reliably assign the auditory pseudowords "bouba" and "kiki" to rounded and pointed shapes, respectively. Previously we showed that representational dissimilarity matrices (RDMs) of the shape ratings of auditory pseudowords correlated significantly with RDMs of acoustic parameters reflecting spectro-temporal variations; the ratings also correlated significantly with voice quality features. Here, participants rated auditory pseudowords on scales representing categorical opposites across seven meaning domains, including shape. Examination of the relationships of the perceptual ratings to spectro-temporal and vocal parameters of the pseudowords essentially replicated our previous findings for shape while varying patterns emerged for the other domains. Thus, the spectro-temporal and vocal properties of spoken pseudowords contribute differentially to sound-symbolic mapping depending on the meaning domain.

Accelerating rate of human impact and environmental change severely affects marine biodiversity and increases the urgency to implement the Convention on Biological Diversity (CBD) 30×30 plan for conserving 30% of sea areas by 2030. However, area-based conservation targets are complex to identify in a 3-dimensional (3D) ocean where deep-sea features such as seamounts have been seldom studied mostly due to challenging methodologies to implement at great depths. Yet, the use of emerging technologies, such as environmental DNA combined with modern modeling frameworks, could help address the problem. We collected environmental DNA, echosounder acoustic, and video data at 15 seamounts and deep island slopes across the Coral Sea. We modeled 7 fish community metrics and the abundances of 45 individual species and molecular operational taxonomic units (MOTUs) in benthic and pelagic waters (down to 600-m deep) with boosted regression trees and generalized joint attribute models to describe biodiversity on seamounts and deep slopes and identify 3D protection solutions for achieving the CBD area target in New Caledonia (1.4 million km 2 ). We prioritized the identified conservation units in a 3D space, based on various biodiversity targets, to meet the goal of protecting at least 30% of the spatial domain, with a focus on areas with high biodiversity. The relationship between biodiversity protection targets and the spatial area protected by the solution was linear. The scenario protecting 30% of each biodiversity metric preserved almost 30% of the considered spatial domain and accounted for the 3D distribution of biodiversity. Our study paves the way for the use of combined data collection methodologies to improve biodiversity estimates in 3D structured marine environments for the selection of conservation areas and for the use of biodiversity targets to achieve area-based international targets., (© 2024 The Author(s). Conservation Biology published by Wiley Periodicals LLC on behalf of Society for Conservation Biology.)

Introduction: Childhood and adolescence are essential stages in the development of voice and speech quality; therefore, it is essential to understand the vocal changes that occur during this period. Frequency-based measurement methods like cepstral measurements stand out among the methods described, which are able to identify f o and estimate the periodicity and noise in the acoustic wave without establishing individual cycles of the sound wave., Methods: Two hundred seventy-one recordings (128 female and 131 male) from children and adolescents aged 5 to 18 years with no vocal complaints were analyzed. Three speech-language pathologists assessed the vocal quality and determined as appropriate for the age. The recordings were divided into six age groups (G1:5-7; G2:8-9; G3:10-11; G4:12; G5:13-15 and G6:16-18 years old). Sustained production of the vowel /a/ were inspected and edited using the PRAAT software. Then, it was extract de Cepstrum Peak Prominence Smoothed (CPPS) using a script in the same software. A Two-way ANOVA was applied to investigate the effect of sex, age and sex*age interaction, followed by Bonferroni's correction for each gender separately. Finally, the Student's t test for independent samples was performed to compare genders within each age group., Results: Male children and adolescents from G5 and G6 had higher CPPS measures than G1, G2 and G3 (P ≤ 0.001). In addition, G6 also had higher values than G4 (P ≤ 0.001). There was no difference between age groups in the female group. In turn, sex differences were reported from 12 years of age onwards, with higher CPPS values found for male participants compared to female participants (P ≤ 0.01)., Conclusion: Vocal changes that usually occur from childhood to adolescence are reflected in the acoustic CPPS measure in males, resulting in higher values in the 13 to 18 years old. On the other hand, no changes in CPPS values were observed in the age groups of female participants. Males have higher CPPS values than females and that sex differences are reported after 12 years of age., (Copyright © 2022 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

In the acoustics of musical instruments with a resonator body, the aging of the wood leads to the improvement of the acoustic properties due to increasing the crystallinity of wood. This phenomenon could be explained by the fact that wood is a complex product based on three-dimensional polymer chains of carbohydrates, its aging being closely related to covalent cross-linking and scission of polymer chains. The aim of this study was to evaluate at a multiscale the changes produced artificial aging of tone wood by measuring the acoustic, mechanical and chemical parameters. The spruce and maple wood samples were investigated before and after exposure to ultraviolet (UV) radiation, through the tensile test, the time-of-flight method (TOF), the analysis of the wood color and the determination of the chemical fingerprint through Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The obtained results showed that the effects of artificial aging are manifested at the chemical level where the crystallinity increases up to the acoustic level, depending on the wood species and their quality class. These results are relevant for musical instrument manufacturers to find treatments that lead to superior acoustic properties., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Aim: The purpose of this study was to evaluate the improvement in acoustic and nasalance in patients with maxillary obturators, using PRAAT software., Materials and Methods: The current study comprised a total of 16 patients who had acquired maxillary defects. Regardless of gender, the age range of these patients was 40-75 years old. The total number of patients were randomly divided into two groups (8 participants in each group) namely (A) Interim obturator group and (B) Definitive obturator group. Analysis was done using PRAAT software for two speech parameters namely nasalance and acoustic value. The first stage of speech analysis was completed without the use of an obturator, whereas the second stage involved inserting the obturator on the same day. The third stage occurred 2 months after the usage of the obturator. The data were recorded and statistically analyzed., Results: In definitive obturator group, mean value for relative nasalance increases from before prosthesis (60.93 ± 3.34 db) to after 2 months of prosthesis (70.53 ± 2.24 db) while in interim group increased from 57.55 ± 4.31 db (before prosthesis) to 63.77 ± 3.66 db (after 2 months of prosthesis). This improvement was more marked with definitive obturator than interim obturator. The F2-F1 comparative mean value changed from before prosthesis to immediate after prosthesis in definitive group for /a/, /e/, and /u/ vowels was 9.77, 22, and 24.38, respectively. F2-F1 comparative mean changes from before prosthesis to after 2 months of prosthesis was 102, 75.75, and 87 for three vowels /a/, /e/, and /u/, respectively. Similarly, F2-F1 mean value in interim prosthesis group changes from before to after 2 months of prosthesis was 62.25, 27.62, and 1.75 for /a/, /e/, and /u/, respectively., Conclusion: On conclusion, the definitive obturators were well-tolerated by patients with major defects and the results show that maxillary resection significantly impairs speech and definitive obturator rehabilitation is effective in regaining nasalance and acoustic speech components., Clinical Significance: Speech disorders in maxillectomy patients are a significant clinical issue since the surgery results in hypernasality, which impairs a person's ability to understand their own speech. Either surgical techniques or prosthetic rehabilitation are used to correct these abnormalities. Certain people require prosthetics to fix their speech even after surgery. How to cite this article: Bhushan P, Thenumkal E, Khosla E, et al. Assessment of Acoustic and Nasalance Improvement in Maxillary Obturator Patients Using PRAAT Software: An In Vivo Study. J Contemp Dent Pract 2024;25(7):656-660.

Objectives, Purpose, or Aim: The study aimed to decrease noise levels in the ICU, anticipated to have adverse effects on both patients and staff, by implementing enhancements in acoustic design., Background: Recognizing ICU noise as a significant disruptor of sleep and a potential hindrance to patient recovery, this study was conducted at a 40-bed ICU in Fiona Stanley Hospital in Perth, Australia., Methods: A comprehensive mixed-methods approach was employed, encompassing surveys, site analysis, and acoustic measurements. Survey data highlighted the importance of patient sleep quality, emphasizing the negative impact of noise on work performance, patient connection, and job satisfaction. Room acoustics analysis revealed noise levels ranging from 60 to 90 dB(A) in the presence of patients, surpassing sleep disruption criteria., Results: Utilizing an iterative 3D design modeling process, the study simulated significant acoustic treatment upgrades. The design integrated effective acoustic treatments within patient rooms, aiming to reduce noise levels and minimize transmission to adjacent areas. Rigorous evaluation using industry-standard acoustic software highlights the design's efficacy in reducing noise transmission in particular. Additionally, cost implications were examined, comparing standard ICU construction with acoustically treated options for new construction and refurbishment projects., Conclusions: This study provides valuable insights into design-based solutions for addressing noise-related challenges in the ICU. While the focus is on improving the acoustic environment by reducing noise levels and minimizing transmission to adjacent areas. It is important to clarify that direct measurements of patient outcomes were not conducted. The potential impact of these solutions on health outcomes, particularly sleep quality, remains a crucial aspect for consideration., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Purpose: Processing real-world sounds requires acoustic and higher-order semantic information. We tested the theory that individuals with autism spectrum disorder (ASD) show enhanced processing of acoustic features and impaired processing of semantic information., Methods: We used a change deafness task that required detection of speech and non-speech auditory objects being replaced and a speech-in-noise task using spoken sentences that must be comprehended in the presence of background speech to examine the extent to which 7-15 year old children with ASD (n = 27) rely on acoustic and semantic information, compared to age-matched (n = 27) and IQ-matched (n = 27) groups of typically developing (TD) children. Within a larger group of 7-15 year old TD children (n = 105) we correlated IQ, ASD symptoms, and the use of acoustic and semantic information., Results: Children with ASD performed worse overall at the change deafness task relative to the age-matched TD controls, but they did not differ from IQ-matched controls. All groups utilized acoustic and semantic information similarly and displayed an attentional bias towards changes that involved the human voice. Similarly, for the speech-in-noise task, age-matched-but not IQ-matched-TD controls performed better overall than the ASD group. However, all groups used semantic context to a similar degree. Among TD children, neither IQ nor the presence of ASD symptoms predict the use of acoustic or semantic information., Conclusion: Children with and without ASD used acoustic and semantic information similarly during auditory change deafness and speech-in-noise tasks., (© 2023. The Author(s).)

Background: Heart failure (HF) contributes greatly to morbidity, mortality, and health care costs worldwide. Hospital readmission rates are tracked closely and determine federal reimbursement dollars. No current modality or technology allows for accurate measurement of relevant HF parameters in ambulatory, rural, or underserved settings. This limits the use of telehealth to diagnose or monitor HF in ambulatory patients., Objective: This study describes a novel HF diagnostic technology using audio recordings from a standard mobile phone., Methods: This prospective study of acoustic microphone recordings enrolled convenience samples of patients from 2 different clinical sites in 2 separate areas of the United States. Recordings were obtained at the aortic (second intercostal) site with the patient sitting upright. The team used recordings to create predictive algorithms using physics-based (not neural networks) models. The analysis matched mobile phone acoustic data to ejection fraction (EF) and stroke volume (SV) as evaluated by echocardiograms. Using the physics-based approach to determine features eliminates the need for neural networks and overfitting strategies entirely, potentially offering advantages in data efficiency, model stability, regulatory visibility, and physical insightfulness., Results: Recordings were obtained from 113 participants. No recordings were excluded due to background noise or for any other reason. Participants had diverse racial backgrounds and body surface areas. Reliable echocardiogram data were available for EF from 113 patients and for SV from 65 patients. The mean age of the EF cohort was 66.3 (SD 13.3) years, with female patients comprising 38.3% (43/113) of the group. Using an EF cutoff of ≤40% versus >40%, the model (using 4 features) had an area under the receiver operating curve (AUROC) of 0.955, sensitivity of 0.952, specificity of 0.958, and accuracy of 0.956. The mean age of the SV cohort was 65.5 (SD 12.7) years, with female patients comprising 34% (38/65) of the group. Using a clinically relevant SV cutoff of <50 mL versus >50 mL, the model (using 3 features) had an AUROC of 0.922, sensitivity of 1.000, specificity of 0.844, and accuracy of 0.923. Acoustics frequencies associated with SV were observed to be higher than those associated with EF and, therefore, were less likely to pass through the tissue without distortion., Conclusions: This work describes the use of mobile phone auscultation recordings obtained with unaltered cellular microphones. The analysis reproduced the estimates of EF and SV with impressive accuracy. This technology will be further developed into a mobile app that could bring screening and monitoring of HF to several clinical settings, such as home or telehealth, rural, remote, and underserved areas across the globe. This would bring high-quality diagnostic methods to patients with HF using equipment they already own and in situations where no other diagnostic and monitoring options exist., (©Martin Huecker, Craig Schutzman, Joshua French, Karim El-Kersh, Shahab Ghafghazi, Ravi Desai, Daniel Frick, Jarred Jeremy Thomas. Originally published in JMIR Cardio (https://cardio.jmir.org), 26.06.2024.)

Objectives: Face masks have become important after the pandemic, but the change in voice when wearing a face mask is still unclear. The study investigated the effect of face masks on the acoustic and perceptual characteristics of voice quality associated with young and older Chinese adults., Methods: Voice samples of the sustained vowel /a/ and continuous speech produced by 44 older and 61 young adults with and without an ASTM level-3 surgical face mask were recorded and analyzed. Perceptual and acoustic parameters including mean fundamental frequency (F0) and intensity, perturbation measures (jitter and shimmer), harmonic-to-noise ratio (HNR), smoothed cepstral peak prominence (CPPs), and long-term average spectrum (LTAS) measures were obtained and compared., Results: When comparing masked to unmasked voices, for both male and female speakers, F0 and intensity showed no significant changes, except for F0 of continuous speech, which increased significantly. Meanwhile, perturbation measures such as jitter and shimmer were reduced, while HNR and CPPs increased. In addition, LTAS measures included low-frequency mean spectral energy (MSE), high-frequency MSE, and spectral tilt (ST), which were different. For perceptual measures, the overall grade of dysphonia, and roughness were reduced, except for the breathiness among older male speakers, while the other vocal qualities were not changed. Between young and older speakers, significant differences in shimmer, CPPs, and perceived breathiness among male speakers, and low-frequency MSE among female speakers were found., Conclusion: Wearing a surgical mask appeared to change the perceived voice quality. This is supported by the change in perturbation and LTAS measures, and HNR and CPPs values. In addition, some differences between young and older adults were observed. Oral Communication effectiveness may be affected when wearing surgical masks due to changes in voice quality. Additionally, clinicians need to exercise hightened caution in evaluating the voice quality of clients when wearing face masks., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

Urban dynamics and anthropogenic interventions led to an increase in noise pollution levels, with relevant implications for worldwide healthcare. Structures created to lessen noise pollution from traffic, industry, or other sources are known as noise, sound, or acoustic barriers. The research paper presents a unique design and development of noise barrier with newly composite mix of acoustic panels and vegetative cover augmenting noise attenuation and aesthetics. The barrier provides a symmetrical design around the vertical axis, hence assures utilization of both faces of the barrier. Additionally, the barrier hosts multiple slots to accommodate multi-material acoustic panels based on diverse noise frequencies and environmental conditions. The barrier integrates movable noise caps at 45°, 90°, 180°, and 270° angles for further attenuation and diversion of noise. A drip irrigation system within the soil box ensures optimal plant growth and stability to barrier. Experimental studies showcase the barrier's performance and its effectiveness in diverse noise scenarios. This innovative development provides a comprehensive solution towards noise mitigation through compact, customizable and sustainable green noise barrier., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Precise control of microparticle movement is crucial in high throughput processing for various applications in scalable manufacturing, such as particle monolayer assembly and 3D bio-printing. Current techniques using acoustic, electrical and optical methods offer precise manipulation advantages, but their scalability is restricted due to issues such as, high input powers and complex fabrication and operation processes. In this work, we introduce the concept of capillary wave tweezers, where mm-scale capillary wave fields are dynamically manipulated to control the position of microparticles in a liquid volume. Capillary waves are generated in an open liquid volume using low frequency vibrations (in the range of 10-100 Hz) to trap particles underneath the nodes of the capillary waves. By shifting the displacement nodes of the waves, the trapped particles are precisely displaced. Using analytical and numerical models, we identify conditions under which a stable control over particle motion is achieved. By showcasing the ability to dynamically control the movement of microparticles, our concept offers a simple and high throughput method to manipulate particles in open systems., (© 2024. The Author(s).)

Objective: To identify the accuracy and cut-off values of the cepstral peak prominence (CPP) and cepstral peak prominence-smoothed (CPPS) obtained from different speech tasks, to identify dysphonic voices in Brazilian Portuguese speakers, and to verify the correlation between these measures and the overall severity of dysphonia (OS)., Method: In a study with 376 subjects-277 with dysphonia and 99 controls-we recorded four speech tasks and assessed OS with a visual analog scale. We extracted CPP and CPPS from these recordings and analyzed them using receiver operating characteristic curves to determine cut-off values and other performance metrics (area under curve, accuracy, sensitivity, specificity, predictive values, and likelihood ratios)., Results: CPP values below 28.15 dB (sustained vowel [Ɛ]), 28.77 dB (sustained vowel [a]), 28.58 dB (all Consensus Auditory-Perceptual Evaluation of Voice (CAPE-V) sentences), and CPPS values below 16.42 dB (sustained vowel [Ɛ]), 17.02 dB (sustained vowel [a]), and 11.30 dB (all CAPE-V sentences) are indicative of the presence of dysphonia. CPP VE , CPP VA , CPP CAPE-V , CPP VS , CPPS VE , CPPS VA , CPPS Count , CPPS CAPE-V e CPPS VS , and CPPS VS can explain the variability of OS from 19% to 57.9%., Conclusions: The cut-off values for CPP VE , CPP VA, CPP CAPE-V , CPPS VE, CPPS CAPE-V, and CPPS VA obtained from Praat software can be used as a reference for the clinical voice assessment of Brazilian Portuguese speakers in the respective speech tasks. Analysis of the correlation coefficients of the cepstral measures investigated provided us with a predictive model of OS perception based on each measure/speech task., Competing Interests: Declaration of Competing Interest The authors report no declarations of interest., (Copyright © 2024 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

Coastal seas are under increasing pressure from extreme weather events and sea level rise, resulting in impacts such as changing hydrodynamic conditions, coastal erosion, and marine heat waves. To monitor changes in coastal marine habitats, such as reefs and macrophytes meadows, which add to the resilience of our coasts, consistent, medium- to long-term seafloor observations are needed. This project aims to deliver repeated, high-frequency sonar surveys on a stationary seabed mooring of a specific target area over a period of up to several months. A new stand-alone subsea system, the Sonarlogger, based on a battery pack, low-power logger and a high-resolution scanning sonar, was developed. It allows for long-term deployments with a customisable battery pack, WI-FI download and configurable sleep state. The system has been tested for over 130 days in dynamic coastal environments off the Belgian coast. Combined with auxiliary sensors, such as for measuring currents, waves and turbidity, this system enables comprehensive studies of morphologic changes and changing benthic ecosystems. Moreover, this system has the capacity to provide measurements of coastal environments during storms, where conventional systems may fall short, providing insights into event-based changes of the seafloor., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Author(s).)

Background: Vocal biomarkers, derived from acoustic analysis of vocal characteristics, offer noninvasive avenues for medical screening, diagnostics, and monitoring. Previous research demonstrated the feasibility of predicting type 2 diabetes mellitus through acoustic analysis of smartphone-recorded speech. Building upon this work, this study explores the impact of audio data compression on acoustic vocal biomarker development, which is critical for broader applicability in health care., Objective: The objective of this research is to analyze how common audio compression algorithms (MP3, M4A, and WMA) applied by 3 different conversion tools at 2 bitrates affect features crucial for vocal biomarker detection., Methods: The impact of audio data compression on acoustic vocal biomarker development was investigated using uncompressed voice samples converted into MP3, M4A, and WMA formats at 2 bitrates (320 and 128 kbps) with MediaHuman (MH) Audio Converter, WonderShare (WS) UniConverter, and Fast Forward Moving Picture Experts Group (FFmpeg). The data set comprised recordings from 505 participants, totaling 17,298 audio files, collected using a smartphone. Participants recorded a fixed English sentence up to 6 times daily for up to 14 days. Feature extraction, including pitch, jitter, intensity, and Mel-frequency cepstral coefficients (MFCCs), was conducted using Python and Parselmouth. The Wilcoxon signed rank test and the Bonferroni correction for multiple comparisons were used for statistical analysis., Results: In this study, 36,970 audio files were initially recorded from 505 participants, with 17,298 recordings meeting the fixed sentence criteria after screening. Differences between the audio conversion software, MH, WS, and FFmpeg, were notable, impacting compression outcomes such as constant or variable bitrates. Analysis encompassed diverse data compression formats and a wide array of voice features and MFCCs. Wilcoxon signed rank tests yielded P values, with those below the Bonferroni-corrected significance level indicating significant alterations due to compression. The results indicated feature-specific impacts of compression across formats and bitrates. MH-converted files exhibited greater resilience compared to WS-converted files. Bitrate also influenced feature stability, with 38 cases affected uniquely by a single bitrate. Notably, voice features showed greater stability than MFCCs across conversion methods., Conclusions: Compression effects were found to be feature specific, with MH and FFmpeg showing greater resilience. Some features were consistently affected, emphasizing the importance of understanding feature resilience for diagnostic applications. Considering the implementation of vocal biomarkers in health care, finding features that remain consistent through compression for data storage or transmission purposes is valuable. Focused on specific features and formats, future research could broaden the scope to include diverse features, real-time compression algorithms, and various recording methods. This study enhances our understanding of audio compression's influence on voice features and MFCCs, providing insights for developing applications across fields. The research underscores the significance of feature stability in working with compressed audio data, laying a foundation for informed voice data use in evolving technological landscapes., (©Jessica Oreskovic, Jaycee Kaufman, Yan Fossat. Originally published in JMIR Biomedical Engineering (http://biomsedeng.jmir.org), 15.04.2024.)

Objective: Anticoagulant therapy is a risk factor for repeated intratumoral hemorrhage and acute enlargement of a vestibular schwannoma (VS) with neurological deficits. Therefore, we describe two cases of patients on oral anticoagulant therapy with intratumoral hemorrhage in which anticoagulant therapy prior to surgical resection was discontinued. We also discuss other similar cases from the literature since this is a rare event., Case Reports: We described the two cases of intratumoral hemorrhage in acoustic neurinoma and conducted a literature review of similar cases of patients with intratumoral hemorrhage in acoustic neurinoma who were also on oral anticoagulants. Both patients presented with CN-VII palsy prior to surgery; both also fully re-covered after surgery except for hearing loss on the tumor side. Our literature review found 50 cases of VS (reported as vestibular schwannomas in the literature) with intratumoral hemorrhage. From this total, 11 patients used oral anticoagulant therapy with reported poor outcomes and high mortality; 9 of these 11 cases were reported in the past 20 years. The incidence is expected to rise due to increased use of anticoagulant therapy due to onset of atrial fibrillation, atherosclerosis, and thromboembolism from longer human lifespan., Conclusion: Anticoagulant therapy represents a risk factor for intratumoral hemorrhage and acute enlargement of VS tumor mass with neurological deficits., (Copyright © 2024 Pojskić et et al. This article is available under a Creative Commons License.)

Objective: This study aims to present an approach for the simulation of ultrasound elastic waves propagation in a diverse range of heterogeneous tissue-like viscoelastic materials, including, but not limited to, Kelvin-Voigt, Zener, Maxwell, Burger's, and Maxwell-Wiechert models, while also allowing for modeling highly viscous fluids., Methods: Ultrasound shear wave elastography (SWE) serves as a cost-effective modality for noninvasive, quantitative assessment of soft tissue viscoelastic mechanical properties. To explore tissue viscoelasticity, measuring the shear wave phase velocity in the frequency domain is a common method. In this paper, we employ modeling and numerical simulations to enhance the development of SWE methods. The study employs the staggered grid finite difference (SGFD) method along with recursive calculations of convolution integrals pertinent to linear viscoelastic models., Results: The presented numerical method demonstrates its capability to simulate the propagation of ultrasound elastic waves, both longitudinal and shear, across a broad spectrum of tissue-like viscoelastic heterogeneous materials. The approach successfully accommodates various viscoelastic models without requiring additional modifications in the numerical model, thus enabling a comprehensive exploration of different viscoelastic behaviors commonly observed in diverse tissue types., Conclusion: The developed combination of the SGFD method and recursive calculation of convolution integrals presents a novel and versatile approach in modeling linear viscoelastic tissue-like materials for SWE applications. This method eliminates the need for model-specific adaptations in numerical simulations, thereby offering flexibility for exploring and understanding diverse viscoelastic behaviors inherent in different heterogeneous tissue types, contributing significantly to the advancement of ultrasound SWE for diagnostic purposes., Competing Interests: Conflict of interest All authors declare that they have no conflicts of interest., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

In general, sound waves propagate radially outwards from a point source. These waves will continue in the same direction, decreasing in intensity, unless a boundary condition is met. To arrive at a universal understanding of the relation between frequency and wave propagation within spatial boundaries, we explore the maximum entropy states that are realized as resonant modes. For both circular and polygonal Chladni plates, a model is presented that successfully recreates the nodal line patterns to a first approximation. We discuss the benefits of such a model and the future work necessary to develop the model to its full predictive ability.

Objective: The Coronavirus Disease 2019 (COVID-19) is an ongoing global pandemic and wearing face mask is recommended across the globe to break the transmission chain of infection. The masks available in the market are of different types and materials and tend to alter the voice characteristics of the speaker. This can therefore impair optimal communication and the present study is a systematic review exploring the effect of various masks on voice production parameters., Study Design: Systematic review., Materials and Methods: The titles and abstracts screening was carried out for the inclusion of articles using eight electronic databases spanning the period from 1st January 2020 to 30th April 2021. 10 articles (8 published & 2 in pre-print) that met the inclusion criteria were considered for this systematic review and the pooled age range was 18 -69 years., Results: Three primary studies from the USA, 2 each from Australia & Italy, one each from Brazil, China, and Germany were found to have investigated the influence of wearing N95, KN95, surgical and fabric masks on voice related measures. The users significantly reported vocal fatigue, discomfort, and also perceived voice problems. Attenuation of speech sound amplitude was highest for the transparent mask followed by cloth mask, N95, KN95, and surgical mask., Conclusion: The World Health Organization (WHO) has been repeatedly endorsing the need to use a face mask in the current COVID-19 pandemic. However, for an unintruded voice production, the surgical mask is recommended for everyone, including healthcare professionals when they are not in close contact with patients, and not involved in aerosol-generating procedures. For teachers, doing direct teaching (offline classes), 'surgical mask' can reduce the vocal load of teachers, smoothen the teacher-student interaction and thereby facilitate better learning by the students. Additionally, it would be useful to protect oneself from the risk of developing voice problems by following standard vocal healthcare tips., (Copyright © 2021 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

Introduction: Uroflowmetry is a noninvasive measurement of the volume of urine excreted over time. Conventional uroflowmetry has become the main modality of urine flow measurement within time. However, this method requires the patient to be present in the hospital or healthcare setting, thus sometimes making the patients feel uncomfortable to undergo the examination. This led to multiple measurements which are inconvenient for the patients. Mobile acoustic uroflowmetry (sono-uroflowmetry) has been proposed as an alternative method of urine flow measurement due to its portability. This study aimed to evaluate the accuracy and reliability of sono-uroflowmetry as compared to conventional uroflowmetry., Methods: Electronic databases searching were done using prespecified search strategy to retrieve articles related with uroflowmetry. In addition, hand-search strategy was used to identify additional articles. Studies with participants who had undergone sono-uroflowmetry were included. Voided volume, voiding duration, maximum flow rate, and average flow rate were identified and used to determine the outcomes of measurement. The quality of included articles was conducted using checklist for Diagnostic Test Accuracy Studies by JBI., Results: Initial search yielded 335 articles with four additional papers identified through hand-searching process. Six papers were retrieved and further used in the narrative synthesis. Five studies enrolled male participants, while only one of the papers enrolled female participants as additional subgroup analysis. Therefore, the meta-analysis was performed by using only male participants. Based on the meta-analysis results, there were strong to very strong positive correlation in voided volume, voiding time, average flow, average flow rate, and maximum flow rate between sono and conventional uroflowmetry., Conclusion: Sonouroflowmetry showed significant positive correlations to conventional uroflowmetry, signifying its use as an alternative of conventional uroflowmetry., (© 2024 Wiley Periodicals LLC.)

Background: The potential of biomarkers in the detection of Alzheimer's disease (AD) is prominent. Acoustics may be useful in this context but the evaluation and weighting for specific acoustic parameters on continuous speech is missing. This meta-analysis aimed to explore the significance of acoustic parameters from acoustic speech analysis on continuous speech, as a diagnostic tool for clinical AD., Methods: Applying PRISMA protocol, a comprehensive search was done in MEDLINE, Scopus, Web of Science, and CENTRAL, from 1960 to January 2024. Cross-sectional studies comparing the acoustic speech analysis between AD patients and healthy controls (HC), were taken into account. The bias risk of the included studies were examined via JBI checklist. Using Review Manager v.5.4.1, the mean differences of acoustic speech parameters among AD and HC were weighted, and the pooled analysis and the heterogeneity statistics were conducted., Results: In total, 1112 records (without duplicates) were obtained, and 11 papers with 7 acoustic parameters were included for this study, and 8 from 11 studies were identified with a low level of bias. Five from 7 acoustic parameters revealed significant differences among the two groups (p-values ≤ 0.01), in which for all rate-related and interruption-related acoustic parameters were the most prominent and less in temporal-related acoustic parameters., Conclusions: Although a small number of acoustic parameters on continuous speech could be evaluated in the detection of clinical AD, the greatest potential of acoustic biomarkers for AD appeared to exist in two of three categories. Further contributions of high-quality studies are needed to support evidence for acoustics as biomarkers for AD., Competing Interests: The authors declare no conflict of interest.

Microbubble's mass transfer under external acoustic excitation holds immense potential across various technological fields. However, the current state of acoustic technology faces limitations due to inadequate control over bubble size in liquids under external excitation. Here, we conducted numerical investigations of the mass transfer behavior of microbubbles in liquids under multifrequency acoustic excitations with different frequencies (in the MHz range), pressure amplitudes (in the range of several atmospheric pressures), and amplitude ratios. We identified various pressure threshold regions for the growth of gas bubbles (radii range from a few microns to tens of microns) and observed common intersections between single and multifrequency excitations that enable effective control of the growth intervals and final size of bubbles by adjusting the ratio of pressure amplitude and frequency value. Allocating power to the lower frequency component of multifrequency acoustic excitation is recommended to facilitate mass transfer or diffusion, as small-frequency acoustic excitation has a more significant effect than the higher frequency in the growth region. Our study provides a better understanding of the dynamics of bubbles under complex excitations and has practical implications for developing methods to control and promote bubble-related processes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

While animals navigating the real world face a barrage of sensory input, their brains evolved to perceptually compress multidimensional information by selectively extracting the features relevant for survival. Notably, communication signals supporting social interactions in several mammalian species consist of acoustically complex sequences of vocalisations. However, little is known about what information listeners extract from such time-varying sensory streams. Here, we utilise female mice's natural behavioural response to male courtship songs to identify the relevant acoustic dimensions used in their social decisions. We found that females were highly sensitive to disruptions of song temporal regularity and preferentially approached playbacks of intact over rhythmically irregular versions of male songs. In contrast, female behaviour was invariant to manipulations affecting the songs' sequential organisation or the spectro-temporal structure of individual syllables. The results reveal temporal regularity as a key acoustic cue extracted by mammalian listeners from complex vocal sequences during goal-directed social behaviour., Competing Interests: CP, CV, DB No competing interests declared, (© 2023, Perrodin et al.)

This work involves exploring non-invasive sensor technologies for data collection and preprocessing, specifically focusing on novel thermal calibration methods and assessing low-cost infrared radiation sensors for facial temperature analysis. Additionally, it investigates innovative approaches to analyzing acoustic signals for quantifying coughing episodes. The research integrates diverse data capture technologies to analyze them collectively, considering their temporal evolution and physical attributes, aiming to extract statistically significant relationships among various variables for valuable insights. The study delineates two distinct aspects: cough detection employing a microphone and a neural network, and thermal sensors employing a calibration curve to refine their output values, reducing errors within a specified temperature range. Regarding control units, the initial implementation with an ESP32 transitioned to a Raspberry Pi model 3B+ due to neural network integration issues. A comprehensive testing is conducted for both fever and cough detection, ensuring robustness and accuracy in each scenario. The subsequent work involves practical experimentation and interoperability tests, validating the proof of concept for each system component. Furthermore, this work assesses the technical specifications of the prototype developed in the preceding tasks. Real-time testing is performed for each symptom to evaluate the system's effectiveness. This research contributes to the advancement of non-invasive sensor technologies, with implications for healthcare applications such as remote health monitoring and early disease detection.

The fast development of unmanned aerial vehicles (UAVs), commonly known as drones, has brought a unique set of opportunities and challenges to both the civilian and military sectors. While drones have proven useful in sectors such as delivery, agriculture, and surveillance, their potential for abuse in illegal airspace invasions, privacy breaches, and security risks has increased the demand for improved detection and classification systems. This state-of-the-art review presents a detailed overview of current improvements in drone detection and classification techniques: highlighting novel strategies used to address the rising concerns about UAV activities. We investigate the threats and challenges faced due to drones' dynamic behavior, size and speed diversity, battery life, etc. Furthermore, we categorize the key detection modalities, including radar, radio frequency (RF), acoustic, and vision-based approaches, and examine their distinct advantages and limitations. The research also discusses the importance of sensor fusion methods and other detection approaches, including wireless fidelity (Wi-Fi), cellular, and Internet of Things (IoT) networks, for improving the accuracy and efficiency of UAV detection and identification.

The classical mathematical modeling of ultrasound acoustic bubble is so far using to improve the medical imaging quality. A clear and visible medical ultrasound image relies on bubble's diameter, wavelength and intensity of the scattered sound. A bubble with diameter much smaller than the sound wavelength is regarded as highly efficient source of sound scattering. The dynamical equation for a medical ultrasound bubble is primarily modeled in classical integer-order differential equation. Then a reduction of order technique is used to convert the modeled dynamic equation for the bubble surface into a system of incommensurate fractional-orders. The incommensurate fractional-order values are calculated directly, by using Riemann stability region. On the basis of stability the convergence and accuracy of the numerical scheme is also discussed in detail. It has been found that the system will remain stable and chaotic for the incommensurate values α 1 <0.737 and α 2 <2.80, respectively., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

Efficient thermal insulation materials considerably lower power consumption for heating and cooling of buildings, which in turn minimises CO 2 emissions and improves indoor comfort conditions. However, the selection of suitable insulation materials is governed by several factors, such as the environmental impact, health impact, cost and durability. Additionally, the disposal of used insulation materials is a major factor that affects the selection of materials because some materials could be very toxic for humans and the environment, such as asbestos-containing materials. Therefore, there is a continuous research effort, in both industry and academia, to develop sustainable and affordable insulation materials. In this context, this work aims at utilising the packing industry wastes (cardboard) to develop an eco-friendly insulation layer, which is a biodegradable material that can be disposed of safely after use. Experimentally, wasted cardboard was collected, cleaned, and soaked in water for 24 h. Then, the wet cardboard was minced and converted into past papers, then cast in square moulds and left in a ventilated oven at 75 °C to dry before de-moulding them. The produced layers were subjected to a wide range of tests, including thermal conductivity, acoustic insulation, infrared imaging and bending resistance. The obtained results showed the developed material has a good thermal and acoustic insulation performance. Thermally, the developed material had the lowest thermal conductivity (λ) (0.039 W/m.K) compared to the studied traditional materials. Additionally, it successfully decreased the noise level from 80 to about 58 dB, which was better than the efficiency of the commercial polyisocyanurate layer. However, the bending strength of the developed material was a major drawback because the material did not resist more than 0.6 MPa compared to 2.0 MPa for the commercial polyisocyanurate and 70.0 MPa for the wood boards. Therefore, it is recommended to investigate the possibility of strengthening the new material by adding fibres or cementitious materials., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 Published by Elsevier Ltd.)

With the emergence of various cutting-edge micromachining technologies, lab on a chip is growing rapidly, but it is always a challenge to realize the on-chip separation of living cells from cell samples without affecting cell activity and function. Herein, we report a novel on-chip label-free method for sorting living and dead cells by integrating the hypertonic stimulus and tilted-angle standing surface acoustic wave (T-SSAW) technologies. On a self-designed microfluidic chip, the hypertonic stimulus is used to distinguish cells by producing volume differences between living and dead cells, while T-SSAW is used to separate living and dead cells according to the cell volume difference. Under the optimized operation conditions, for the sample containing 50% of living human umbilical vein endothelial cells (HUVECs) and 50% of dead HUVECs treated with paraformaldehyde, the purity of living cells after the first separation can reach approximately 80%, while after the second separation, it can be as high as 93%; furthermore, the purity of living cells after separation increases with the initial proportion of living cells. In addition, the chip we designed is safe for cells and can robustly handle cell samples with different cell types or different causes of cell death. This work provides a new design of a microfluidic chip for label-free sorting of living and dead cells, greatly promoting the multi-functionality of lab on a chip.

Objectives: To compare the outcomes of bi-weekly voice therapy (BWVT) with weekly voice therapy on perception, acoustics, and quality of life for individuals with muscle tension dysphonia (MTD)., Methods: Thirty participants with MTD were enrolled either BWVT (40 min/session, two sessions per week for 4 weeks) or weekly voice therapy (40 min/session, once a week for 8 weeks). Auditory perceptual ratings, acoustic parameters, and the Mandarin version of the voice-related quality of life questionnaire (MV-RQOL) scores were statistically analyzed before and after treatment., Results: There were significant improvements in the voice qualities such as overall grade, roughness, asthenia, and strain in both groups after treatment. Acoustics analyses showed that fundamental frequency, speaking fundamental frequency, jitter, shimmer, and cepstral peak prominence significantly improved in both groups after treatment. For the MV-RQOL questionnaire scores, the result also demonstrated that both groups felt significant improvements in voice-related quality of life after treatment. However, there were no significant differences between the two groups of treatment effects., Conclusions: Patients with MTD can restore voice qualities and quality of life if they are able to complete a full course of voice therapy, regardless of the intensity of voice therapy. However, if they can receive the BWVT, they would be able to regain their voice faster. The results of this study can be provided as a reference for clinicians when treating patients with MTD., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflicts of interest related to the subject matter or materials discussed in this article., (Copyright © 2023 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

Herein we show an accessible technique based on Faraday waves that assist the rapid assembly of osteoinductive β-Tricalcium phosphate (β-TCP) particles as well as human osteoblast pre-assembled in spheroids. The hydrodynamic forces originating at 'seabed' of the assembly chamber can be used to tightly aggregate inorganic and biological entities at packing densities that resemble those of native tissues. Additionally, following a layer-by-layer assembly procedure, centimeter scaled osteoinductive three-dimensional and cellularized constructs have been fabricated. We showed that the intimate connection between biological building blocks is essential in engineering living system able of localized mineral deposition. Our results demonstrate, for the first time, the possibility to obtain three-dimensional cellularized and acellularized anisotropic constructs using Faraday waves., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)

(1) Background: When a human or animal is recovering from general anesthesia, their medical team uses several behavioral and physiological parameters to assess their emergence from the unconscious state to complete wakefulness. However, the return of auditory and acoustic behaviors indicative of the complete return of consciousness in humans can be difficult to assess in a completely aquatic non-human mammal. Dolphins produce sound using the nasal system while using both passive auditory and active biological sonar (echolocation) to navigate and interrogate their environment. The sounds generated by dolphins, such as whistles and clicks, however, can be difficult to hear when the animal is submerged. (2) Methods: We implemented a system to audibly and visually (i.e., using spectrograms) monitor the underwater acoustic behavior of dolphins recovering from anesthesia. (3) Results: Eleven of the twelve recorded dolphins began echolocating within 92 min (Mean = 00:43:41 HH:MM:SS) following spontaneous respirations. In all cases, the dolphins echolocated prior to whistling (Mean = 04:57:47). The return of echolocation was significantly correlated to the return of the righting reflex (Mean = 1:13:44), a commonly used behavioral indicator of dolphin emergence. (4) Conclusions: We suggest that acoustic monitoring for the onset of click production may be a useful supplement to the established medical and behavioral biomarkers of restoring consciousness following anesthesia in bottlenose dolphins.

Objective: The objective of this study was to compare phonetic outcomes of TOETVA with those of conventional transcervical thyroidectomy(OPEN)., Methods: Retrospective chart review of total 61 patients who received thyroidectomy through OPEN (n = 34) or TOETVA (n = 27) approach in single tertiary center were enrolled., Results: There were significant differences in F0 between the two groups and there were significant changes from different time points within each group in jitter for vowel /i/. Aerodynamically, there were significant differences in mean sound pressure level for vowel /a/ and mean sound pressure level, mean air pressure, and aerodynamic resistance for syllable /pa/ between the two groups. In subjective VHI evaluations, there were no significant differences between the two groups., Conclusion: This study is significant in that it has investigated voices of patients with TOETVA. Results of this study are expected to be useful for voice evaluation, treatment, and prevention in the future., Competing Interests: Conflict of interest statements All authors had no conflict of interest on this study, (Copyright © 2022. Published by Elsevier B.V.)

Background: Very few studies have assessed the presence of a possible correlation between speech variables and limb bradykinesia in patients with Parkinson's disease (PD). The objective of this study was to find correlations between different speech variables and upper extremity bradykinesia under different medication conditions in advanced PD patients., Methods: Retrospective data were collected from a cohort of advanced PD patients before and after an acute levodopa challenge. Each patient was assessed with a perceptual-acoustic analysis of speech, which included several quantitative parameters [i.e., maximum phonation time (MPT) and intensity (dB)]; the Unified Parkinson's Disease Rating Scale (UPDRS) (total scores, subscores, and items); and a timed test (a tapping test for 20 s) to quantify upper extremity bradykinesia. Pearson's correlation coefficient was applied to find correlations between the different speech variables and the tapping rate., Results: A total of 53 PD patients [men: 34; disease duration: 10.66 (SD 4.37) years; age at PD onset: 49.81 years (SD 6.12)] were included. Levodopa intake increased the MPT of sustained phonation ( p < 0.01), but it reduced the speech rate ( p = 0.05). In the defined-OFF condition, MPT of sustained phonation positively correlated with both bilateral mean ( p = 0.044, r -value:0.299) and left ( p = 0.033, r -value:0.314) tapping. In the defined-ON condition, the MPT correlated positively with bilateral mean tapping ( p = 0.003), left tapping ( p = 0.003), and right tapping ( p = 0.008)., Conclusion: This study confirms the presence of correlations between speech acoustic variables and upper extremity bradykinesia in advanced PD patients. These findings suggest common pathophysiological mechanisms., Competing Interests: EMo has received honoraria from Medtronic for consulting and lecturing and has received research grants from Ipsen and Abbott. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Cavallieri, Di Rauso, Gessani, Budriesi, Fioravanti, Contardi, Menozzi, Pinto, Moro, Antonelli and Valzania.)

In a conventional two-dimensional (2D) culture method, cells are attached to the bottom of the culture dish and grow into a monolayer. These 2D culture methods are easy to handle, cost-effective, reproducible, and adaptable to growing many different types of cells. However, monolayer 2D cell culture conditions are far from those of natural tissue, indicating the need for a three-dimensional (3D) culture system. Various methods, such as hanging drop, scaffolds, hydrogels, microfluid systems, and bioreactor systems, have been utilized for 3D cell culture. Recently, external physical stimulation-based 3D cell culture platforms, such as acoustic and magnetic forces, were introduced. Acoustic waves can establish acoustic radiation force, which can induce suspended objects to gather in the pressure node region and aggregate to form clusters. Magnetic targeting consists of two components, a magnetically responsive carrier and a magnetic field gradient source. In a magnetic-based 3D cell culture platform, cells are aggregated by changing the magnetic force. Magnetic fields can manipulate cells through two different methods: positive magnetophoresis and negative magnetophoresis. Positive magnetophoresis is a way of imparting magnetic properties to cells by labeling them with magnetic nanoparticles. Negative magnetophoresis is a label-free principle-based method. 3D cell structures, such as spheroids, 3D network structures, and cell sheets, have been successfully fabricated using this acoustic and magnetic stimuli-based 3D cell culture platform. Additionally, fabricated 3D cell structures showed enhanced cell behavior, such as differentiation potential and tissue regeneration. Therefore, physical stimuli-based 3D cell culture platforms could be promising tools for tissue engineering., (© 2023. Korean Tissue Engineering and Regenerative Medicine Society.)

Molybdenum disulfide (MoS 2 ) has distinctive electronic and mechanical properties which make it a highly prospective material for use as a channel in upcoming nanoelectronic devices. An analytical modeling framework was used to investigate the I-V characteristics of field-effect transistors based on MoS 2 . The study begins by developing a ballistic current equation using a circuit model with two contacts. The transmission probability, which considers both the acoustic and optical mean free path, is then derived. Next, the effect of phonon scattering on the device was examined by including transmission probabilities into the ballistic current equation. According to the findings, the presence of phonon scattering caused a decrease of 43.7% in the ballistic current of the device at room temperature when L = 10 nm. The influence of phonon scattering became more prominent as the temperature increased. In addition, this study also considers the impact of strain on the device. It is reported that applying compressive strain could increase the phonon scattering current by 13.3% at L = 10 nm at room temperature, as evaluated in terms of the electrons' effective masses. However, the phonon scattering current decreased by 13.3% under the same condition due to the existence of tensile strain. Moreover, incorporating a high-k dielectric to mitigate the impact of scattering resulted in an even greater improvement in device performance. Specifically, at L = 6 nm, the ballistic current was surpassed by 58.4%. Furthermore, the study achieved SS = 68.2 mV/dec using Al 2 O 3 and an on-off ratio of 7.75 × 10 4 using HfO 2 . Finally, the analytical results were validated with previous works, showing comparable agreement with the existing literature.

We describe ongoing efforts to better understand the interaction of spoken languages and their physical environments. We begin by briefly surveying research suggesting that languages evolve in ways that are influenced by the physical characteristics of their environments, however the primary focus is on the converse issue: how speech affects the physical environment. We discuss the speech-based production of airflow and aerosol particles that are buoyant in ambient air, based on some of the results in the literature. Most critically, we demonstrate a novel method used to capture aerosol, airflow, and acoustic data simultaneously. This method captures airflow data via a pneumotachograph and aerosol data via an electrical particle impactor. The data are collected underneath a laminar flow hood while participants breathe pure air, thereby eliminating background aerosol particles and isolating those produced during speech. Given the capabilities of the electrical particle impactor, which has not previously been used to analyze speech-based aerosols, the method allows for the detection of aerosol particles at temporal and physical resolutions exceeding those evident in the literature, even enabling the isolation of the role of individual sound types in the production of aerosols. The aerosols detected via this method range in size from 70 nanometers to 10 micrometers in diameter. Such aerosol particles are capable of hosting airborne pathogens. We discuss how this approach could ultimately yield data that are relevant to airborne disease transmission and offer preliminary results that illustrate such relevance. The method described can help uncover the actual articulatory gestures that generate aerosol emissions, as exemplified here through a discussion focused on plosive aspiration and vocal cord vibration. The results we describe illustrate in new ways the unseen and unheard ways in which spoken languages interact with their physical environments., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Everett, Darquenne, Niles, Seifert, Tumminello and Slade.)

Objectives: The voice quality of patients with Coronavirus Disease 2019 (COVID-19) seems to be affected due to lower and upper respiratory involvement. Patient-based voice assessment scales are important clinical measures to diagnose voice disorders and monitor treatment outcomes in COVID-19 patients. This study compared vocal fatigue between COVID-19 patients and those with normal voices. Furthermore, the relationship between vocal fatigue and acoustic voice parameters of COVID-19 patients was evaluated., Methods: This cross-sectional study enrolled 30 laboratory-confirmed patients with COVID-19 (18 males and 12 females) and 30 healthy individuals with normal voices (14 males and 16 females) to compare their respiratory or phonatory parameters. The Persian versions of the Consensus Auditory Perceptual Evaluation of Voice (CAPE-V) and the vocal fatigue index (VFI) were conducted before and after reading the text. The Jitter, shimmer, maximum phonation time, and harmonic-to-noise ratio (HNR) were analyzed by Praat software based on the recorded voices of CAPE-V tasks. The acoustic assessment and VFI questionnaire results were compared between COVID-19 patients and the control group., Results: There were significant differences between COVID-19 patients and their healthy counterparts in all VFI subscales (P < 0.001). Moreover, after reading the text, we found significant differences between the two groups regarding Jitter, shimmer, and HNR of /a/ and /i/ vowels (P < 0.05). Our findings also indicated a significant correlation between symptom improvement with rest and acoustic parameters in all tasks, except the Jitter of /a/ before reading the text., Conclusion: Patients with COVID-19 showed significantly more vocal fatigue than people with normal voices after reading the text. Moreover, there was a significant relationship between Jitter, shimmer, and HNR and the tiredness of voice and physical discomfort subscales of VFI., Competing Interests: DECLARATION OF COMPETING INTEREST The authors have no conflicts of interest to declare., (Copyright © 2023 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

Mating behaviors are sensitive to novel or stressful thermal conditions, particularly for ectothermic organisms. An organism's sensitivity to temperature, which may manifest in altered mating outcomes, can be shaped in part by temperatures experienced during development. Here, we tested how developmental temperature shapes the expression of adult mating-related behaviors across different ambient conditions, with a focus on courtship behavior, mating rates, and mating signals and preferences. To do so, we reared treehoppers under two temperature regimes and then tested the expression of male and female mating behaviors across a range of ambient temperatures. We found that developmental temperature affects the thermal sensitivity of courtship behavior and mating signals for males. However, developmental temperature did not affect the thermal sensitivity of courtship or mate preferences in females. This sex-specific plasticity did not alter the likelihood of mating across ambient temperatures, but it did disrupt how closely mating signals and preferences matched each other at higher ambient temperatures. As a result, developmental temperature could alter sexual selection through signal-preference de-coupling. We further discuss how adult age may drive sex-specific results, and the potential for mismatches between developmental and mating thermal environments under future climate change predictions., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Increasing protein demands directly require additional resources to those presently and recurrently available. Emerging green technologies have witnessed an escalating interest in "Cavitation Processing" (CP) to ensure a non-invasive, non-ionizing and non-polluting extraction. The main intent of this review is to present an integrated summary of cavitation extraction methods specifically applied to food protein sources. Along with a comparative assessment carried out for each type of cavitation model, protein extraction yield and implications on the extracted protein's structural and functional properties. The basic principle of cavitation is due to the pressure shift in the liquid flow within milliseconds. Hence, cavitation emerges similar to boiling; however, unlike boiling (temperature change), cavitation occurs due to pressure change. Characterization and classification of sample type is also a prime candidate when considering the applications of cavitation models in food processing. Generally, acoustic and hydrodynamic cavitation is applied in food applications including extraction, brewing, microbial cell disruption, dairy processing, emulsification, fermentation, waste processing, crystallisation, mass transfer and production of bioactive peptides. Micro structural studies indicate that shear stress causes disintegration of hydrogen bonds and Van der Waals interactions result in the unfolding of the protein's secondary and/or tertiary structures. A change in the structure is not targeted but rather holistic and affects the physicochemical, functional, and nutritional properties. Cavitation assisted extraction of protein is typically studied at a laboratory scale. This highlights limitations against the application at an industrial scale to obtain potential commercial gains., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Although positive photobiomodulation response on wound healing, tissue repair, and therapeutic treatment has been widely reported, additional works are still needed to understand its effects on human blood. This research carried out acoustic measurements using A-scan (GAMPT) ultrasonic techniques to elucidate the photobiomodulation effects on in vitro human blood samples as therapeutic treatment measures. The human blood samples were irradiated using a 532-nm laser with different output laser powers (60 and 80 mW) at various exposure times. The ultrasonic velocity measured in the human blood samples after laser irradiation showed significant changes, most of which were within the acceptance limit for soft tissues (1570 [Formula: see text] 30 m/s). Abnormal cells (echinocyte and crenation) were observed due to excessive exposure during laser treatment., (© 2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)

Objectives: To examine the effects of short-term and long-term engagement with structured choral singing on vocal function and quality of life outcomes in older adults., Methods: Two groups of older adult singers over 55 years, one with fewer than 4 semesters and one with 4 or more semesters singing in a chorale, were assessed at 3 time points: baseline, after 1 semester of singing, and either after 1 semester of rest or after 1 semester of rest and 1 semester more of singing. Acoustic and aerodynamic measures, voice-related quality of life ratings, and measures of singing accuracy were obtained. Percent change between time points were calculated to determine three outcomes: improvement, lack of change, or worsening of measures across time., Results: Long-term average spectrum (LTAS), difference in first and second harmonics and estimated subglottic pressure were significantly more likely to improve after a semester of singing with less experience singers, and LTAS continued to improve after a semester of rest. Flow was significantly more likely to improve with more singing experience after a semester of singing. Aerodynamic variables consistently changed in more experienced singers and improvement was maintained over the three visits. No significant changes occurred over time for singing accuracy for any singer type. Self-perception of singing voice continued to improve with more singing experience., Conclusions: This study demonstrated that for older adults in good health, regular singing provided a mechanism for maintaining speaking voice over time., (Copyright © 2023 The Voice Foundation. Published by Elsevier Inc. All rights reserved.)

The vibrational modes of semiconductor and metal nanostructures occur in the MHz to GHz frequency range, depending on dimensions. These modes are at the heart of nano-optomechanical devices, and understanding how they dissipate energy is important for applications of the devices. In this paper ultrafast transient absorption microscopy has been used to examine the breathing modes of a single gold nanoplate, where up to four overtones were observed. Analysis of the frequencies and amplitudes of the modes using a simple continuum mechanics model shows that the system behaves as a free plate, even though it is deposited onto a surface with no special preparation. The overtones decay faster than the fundamental mode, which is not predicted by continuum mechanics calculations of mode damping due to radiation of sound waves. Possible reasons for this effect include frequency dependent thermoelastic effects in the nanoplate, and/or flow of acoustic energy out of the excitation region., Competing Interests: The authors declare that there are no conflicts of interest for the present manuscript., (© 2023 The Authors.)