1. A nitroreductase responsive probe for early diagnosis of pulmonary fibrosis disease.
- Author
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Peng S, Liang Y, Zhu H, Wang Y, Li Y, Zhao Z, Li Y, Zhuang R, Huang L, Zhang X, and Guo Z
- Subjects
- Animals, Mice, Bleomycin, Lung diagnostic imaging, Lung pathology, Lung metabolism, Humans, Disease Models, Animal, Tissue Distribution, Male, Fluorine Radioisotopes, Radiopharmaceuticals, Nitroreductases metabolism, Positron Emission Tomography Computed Tomography, Early Diagnosis, Idiopathic Pulmonary Fibrosis diagnostic imaging, Idiopathic Pulmonary Fibrosis diagnosis, Idiopathic Pulmonary Fibrosis metabolism, Idiopathic Pulmonary Fibrosis pathology, Idiopathic Pulmonary Fibrosis chemically induced
- Abstract
Idiopathic pulmonary fibrosis (IPF) is a serious interstitial lung disease. However, the definitive diagnosis of IPF is impeded by the limited capabilities of current diagnostic methods, which may fail to capture the optimal timing for treatment. The main goal of this study is to determine the feasibility of a nitroreductase (NTR) responsive probe,
18 F-NCRP, for early detection and deterioration monitoring of IPF.18 F-NCRP was obtained with high radiochemical purity (>95 %). BLM-injured mice were established by intratracheal instillation with bleomycin (BLM) and characterized through histological analysis. Longitudinal PET/CT imaging, biodistribution study and in vitro autoradiography were performed. The correlations between the uptake of18 F-NCRP and mean lung density (tested by CT), as well as histopathological characteristics were analyzed. In PET imaging study,18 F-NCRP exhibited promising efficacy in monitoring the progression of IPF, which was earlier than CT. The ratio of uptake in BLM-injured lung to control lung increased from 1.4-fold on D15 to 2.2-fold on D22. Biodistribution data showed a significant lung uptake of18 F-NCRP in BLM-injured mice. There was a strong positive correlation between the18 F-NCRP uptake in the BLM-injured lungs and the histopathological characteristics. Given that,18 F-NCRP PET imaging of NTR, a promising biomarker for investigating the underlying pathogenic mechanism of IPF, is attainable as well as desirable, which might lay the foundation for establishing an NTR-targeted imaging evaluation system of IPF., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2024
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