Search

A major challenge in forensic anthropology and bioarcheology is the development of fast and effective methods for sorting commingled remains. This study assesses how portable laser-induced breakdown spectroscopy (LIBS) can be used to group skeletal remains based on their elemental profiles. LIBS spectra were acquired from the remains of 45 modern skeletons, with a total data set of 8388 profiles from 1284 bones. Spectral feature selection was conducted to reduce the spectral profiles to the peaks exhibiting the highest variation among individuals. Emission lines corresponding to 9 elements (Ca, P, C, K, Mg, Na, Al, Ba, and Sr) were found important for classification. Linear discriminant analysis (LDA) was concurrently used to classify each spectral profile. From the 45 individuals, each LIBS spectrum was successfully sorted to its corresponding skeleton with an average accuracy of 87%. These findings indicate that variation exists among the LIBS profiles of individuals' skeletal remains, highlighting the potential for portable LIBS technology to aid in the sorting of commingled remains.

Anatomists have been attempting to standardize anatomical terminology of the human body and in doing so created the Terminologia Anatomica as a standard language of anatomy. Despite developments such as the Terminologia Anatomica, a lack of consistency of anatomical terminology is still seen across and within disciplines. This study explores the variation in terminology for the hip bone through a literature review. In total, ten terms used to describe the hip bone were searched in PubMed, JSTOR, and EBSCO databases: "coxal bone," "hip bone," "innominate bone," "os coxa," "os coxae," "ossa coxa," "ossa coxae," "os innominatum," "os pelvicum," and "pelvic bone." Results found that the terms "hip bone" and "pelvic bone" were the most commonly used terms, with the most frequently used discipline being medicine. However, we argue against the use of these terms in favor of "coxal bone" or "innominate bone." "Hip bone" should be avoided due to its colloquial nature, and "pelvic bone" is not specific enough to the structure of the hip. Latin terms are often incorrectly conjugated (e.g., "os coxae"); therefore, the English equivalent should be used when possible. Regardless, standardization of anatomical terminology should be adopted through consensus by practitioners, researchers, and stakeholders., (© 2023. The Author(s), under exclusive licence to Japanese Association of Anatomists.)

The postmortem microbiome has recently moved to the forefront of forensic research, and many studies have focused on the idea that predictable fluctuations in decomposer communities could be used as a "microbial clock" to determine time of death. Commonly, the oral microbiome has been evaluated using 16S rRNA gene sequencing to assess the changes in community composition throughout decomposition. We sampled the hard palates of three human donors over time to identify the prominent members of the microbiome. This study combined 16S rRNA sequencing with whole metagenomic (MetaG) and metatranscriptomic (MetaT) sequencing and culturing methodologies in an attempt to broaden current knowledge about how these postmortem microbiota change and might function throughout decomposition. In all four methods, Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes were the dominant phyla, but their distributions were insufficient in separating samples based on decomposition stage or time or by donor. Better resolution was observed at the level of genus, with fresher samples from decomposition clustering away from others via principal components analysis (PCA) of the sequencing data. Key genera in driving these trends included Rothia ; Lysinibacillus , Lactobacillus , Staphylococcus , and other Firmicutes; and yeasts including Candida and Yarrowia . The majority of cultures (89%) matched to sequences obtained from at least one of the sequencing methods, while 11 cultures were found in the same samples using all three methods. These included Acinetobacter gerneri , Comamonas terrigena , Morganella morganii , Proteus vulgaris , Pseudomonas koreensis , Pseudomonas moraviensis , Raoutella terrigena , Stenotrophomonas maltophilia , Bacillus cereus , Kurthia zopfii , and Lactobacillus paracasei . MetaG and MetaT data also revealed many novel insects as likely visitors to the donors in this study, opening the door to investigating them as potential vectors of microorganisms during decomposition. The presence of cultures at specific time points in decomposition, including samples for which we have MetaT data, will yield future studies tying specific taxa to metabolic pathways involved in decomposition. Overall, we have shown that our 16S rRNA sequencing results from the human hard palate are consistent with other studies and have expanded on the range of taxa shown to be associated with human decomposition, including eukaryotes, based on additional sequencing technologies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Ashe, Comeau, Zejdlik and O’Connell.)