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Sepsis caused by infection could induce systemic inflammation and various organ damages. Bioactivities of peptides derived from eggs were proved by lots of researchers; hence they might be further developed for therapeutic use. In this study, the hydrolysate named "PEP" was obtained by enzymatic hydrolysis and purification. Rats were divided into control, LPS, PEP1 and PEP2 groups. The rat model was established using lipopolysaccharide (LPS) at 10 mg/kg by intraperitoneal injection. The results of blood counts, pro-inflammatory cytokines (TNF-α and IL-6), BUN and Cr levels and pathological damage in tissue or serum suggested that PEP alleviated LPS-induced injuries. Plasma metabolites of PEP were analyzed and identified using UPLC-MS/MS, which revealed that seven pathways might play critical roles in the protection process, involved in oxidative stress, energy and amino acid metabolism. Our data suggest that PEP ameliorated LPS-induced acute liver and kidney injuries, which is helpful for further development and research., Competing Interests: Declaration of competing interest The authors report no conflict of interest., (Copyright © 2022 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)

Objective: To evaluate the role of lncRNA uc003fir in the progression and development of preeclampsia (PE)., Design: Paired test using placental tissue collected from PE women and normally pregnant women., Setting: First Hospital of Soochow University, Suzhou, China., Population: 38 women with PE versus 42 normally pregnant women matched maternal and gestational age with the same-size ratio., Methods: Placental samples were analyzed using a human lncRNA microarray, and then the lncRNA uc003fir was validated by q-PCR. MTT, cell scratch, and transwell invasion assays were performed regarding biological functions., Main Outcome Measures: LncRNA uc003fir promoted CCL5 expression and negatively affected the proliferation and migration of HTR-8/SVneo cells., Results: LncRNA uc003fir was significantly over-expressed in PE compared to the control. The chemokine CCL5 was directly regulated by lncRNA uc003fir through transfection of over-expression plasmid of lncRNA uc003fir in HTR-8/SVneo cells, accompanied by up-regulation of lncRNA uc003fir in preeclamptic placental vessels and vessel-free tissue. In detecting the biological function of lncRNA uc003fir on trophoblast cells, over-expression of lncRNA uc003fir increased proliferation, migration, and invasion of HTR-8/SVneo cells, whereas the knockdown inhibited., Conclusion: LncRNA uc003fir was dysregulated in placental tissue in PE. LncRNA uc003fir regulated CCL5 directly, which may mediate the recruitment of pro-inflammatory cytokines released from monocytes and other leukocytes. Additionally, lncRNA uc003fir may interact with microRNAs to affect the invasion of trophoblast cells into myometrium, indicating that lncRNA uc003fir could be considered as a target in PE., (Copyright © 2018 International Society for the Study of Hypertension in Pregnancy. Published by Elsevier B.V. All rights reserved.)

Objective: To study the effectiveness of dexmedetomidine used for general anesthesia maintenance in children undergoing odontotherapy in day-surgery operating room in reducing the incidence of emergence agitation (EA)., Methods: Eighty children undergoing odontotherapy and under general anesthesia in day-surgery operating room were randomized into two groups, group A (n=40) and group B (n=40). Each patient in group A was administered with a bolus dose of dexmedetomidine (1.0 μg·kg⁻¹, saline diluted to 10 mL) pump-infused after intubation and a maintenance dose of 0.1-0.4 mL·(kg·h)⁻¹ followed-up until 45 min before the end of operation. Each patient in group B was administered with a bolus dose of normal saline 10 mL pump-infused after intubation and maintenance dose of 0.1-0.4 mL·(kg·h)⁻¹ followed-up until 45 min before the end of operation. Gender, age, weight, physical status according to the American Society of Anesthesiologists, perioperative heart rate (HR), mean arterial pressure (MAP), pulse oxygen saturation (SpO₂), sufentanil dosage, duration of surgery, time of extubation, time of regaining consciousness, and time to reach modified Aldrete's score≥12 were recorded. Behavior in postanesthesia care unit was rated on the four-point agitation scale., Results: Compared with group B, decreases were observed in HR and MAP at the beginning of operation, in 10 and 30 min, 1 and 2 h after the beginning of operation, and after extubation of group A (P<0.05). Sufentanil dosage and incidence of EA during recovery of group A were also lower than those of group B (P<0.05). Time to regain consciousness and time to reach modified Aldrete's score≥12 of group A were longer than those of group B (P<0.05). No statistical difference was observed between other indexes of the two groups., Conclusions: As an anesthetic used for general anesthesia maintenance in children undergoing odontotherapy in day-surgery operating room, dexmedetomidine results in low incidence of EA during recovery and more stable vital signs.