1. Transcription Factor VlbZIP14 Inhibits Postharvest Grape Berry Abscission by Directly Activating VlCOMT and Promoting Lignin Biosynthesis.
- Author
-
Yu P, Li S, Sun Y, Meng X, Shi Q, Zhao X, and Yu Y
- Subjects
- Chitosan pharmacology, Sulfur Dioxide pharmacology, Cell Wall metabolism, Cell Wall drug effects, Promoter Regions, Genetic, Vitis drug effects, Vitis genetics, Vitis growth & development, Vitis metabolism, Lignin biosynthesis, Fruit drug effects, Fruit growth & development, Fruit metabolism, Fruit genetics, Plant Proteins genetics, Plant Proteins metabolism, Gene Expression Regulation, Plant drug effects, Transcription Factors metabolism, Transcription Factors genetics
- Abstract
Sulfur dioxide (SO
2 ) is the most effective preservative for table grapes as it reduces the respiratory intensity of berries and inhibits mold growth. However, excessive SO2 causes berry abscission during storage, resulting in an economic loss postharvest. In this study, grapes were exogenously treated with SO2 , SO2 + 1.5% chitosan, SO2 + 1.5% eugenol, and SO2 + eugenol-loaded chitosan nanoparticles (SN). In comparison to SO2 treatment, SN treatment reduced the berries' abscission rate by 74% while maintaining the quality of the berries. Among the treatments, SN treatment most effectively inhibited berry abscission and maintained berry quality. RNA-sequencing (RNA-seq) revealed that SN treatment promoted the expression of genes related to cell wall metabolism. Among these genes, VlCOMT was detected as the central gene, playing a key role in mediating the effects of SN. Dual luciferase and yeast one-hybrid (Y1H) assays demonstrated that VlbZIP14 directly activated VlCOMT by binding to the G-box motif in the latter's promoter, which then participated in lignin synthesis. Our results provide key insights into the molecular mechanisms underlying the SN-mediated inhibition of berry abscission and could be used to improve the commercial value of SO2 -treated postharvest table grapes.- Published
- 2024
- Full Text
- View/download PDF