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Considerable efforts that isolate and characterize degrading bacteria for polycyclic aromatic hydrocarbons (PAHs) have focused on contaminated environments so far. Here we isolated three distinctive pyrene (PYR)-degrading bacteria from a paddy soil that was not contaminated with PAHs. These included a novel Bacillus sp. PyB-9 and efficient degraders, Shigella sp. PyB-6 and Agromyces sp. PyB-10. All three strains could utilize naphthalene, phenanthrene, anthracene, fluoranthene and PYR as sole carbon sources, and degraded PYR in a range of temperatures (27-37 °C) and pH (5-8). Strains PyB-6 and PyB-10 almost completely degraded 50 mg L -1 PYR within 15 days, and 75.5% and 98.9% of 100 mg L -1 PYR in 27 days, respectively. The kinetics of PYR biodegradation was well represented by the Gompertz model. Ten and twelve PYR metabolites were identified in PYR degradation process by strains PyB-6 and PyB-10, respectively. Chemical analyses demonstrated that the degradation mechanisms of PYR were the same for strains PyB-6 and PyB-10 with initial dioxygenation mainly on C-4,5 positions of PYR. The degradation of 4,5-phenanthrenedicarboxylic acid was branched to 4-phenanthrenecarboxylic acid pathway and 5-hydroxy-4-phenanthrenecarboxylic acid pathway, both of which played important roles in PYR degradation by strains PyB-6 and PyB-10. To our knowledge, Shigella sp. and Agromyces sp. were found for the first time to possess the capability for PAHs degradation. These findings contributed to upgrading the bank of microbial resource and knowledge on PAH biodegradation., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)

In recent years, the survival and development of traditional villages in China have been serious challenges. Rural tourism is regarded as an important way to solve rural problems, and the combination of rural culture and tourism has become a new power point for rural development. Therefore, it is necessary to explore the spatial distribution structure between traditional villages and rural tourism. In this paper, rural tourism was represented by the rural tourism characteristic village (RTCV), and Henan Province, China, was taken as a study area to analyze the distribution pattern and spatial correlation of rural tourism and traditional village (TV) and discuss the relationship between the spatial correlation and regional natural environment and socioeconomic factors. The results show that the coupling of the spatial correlation between RTCVs and TVs in Henan was clear. They could be divided into 5 regions based on geographical factors. In addition, the research summarized 4 typical spatial structures between TVs and RTCVs in Henan based on the regional symbiosis theory, and the spatial pattern formation mechanism of TVs and RTCVs was discussed based on three driving mechanisms. The spatial structure of the two can provide reference value for other developing countries and regions to achieve sustainable rural development., (© 2023. The Author(s).)

Background: The recombination-activating gene 1 (RAG1) protein is essential for the V (variable)-D (diversity)-J (joining) recombination process. Mutations in RAG1 have been reported to be associated with several types of immune disorders. Typical clinical features driven by RAG1 variants include persistent infections, severe lymphopenia, and decreased immunoglobulin levels ., Case Presentation: In this study, a 2-month-24-days-old infant with recurrent fever was admitted to our hospital with multiple infections and absence of T and B lymphocytes. The infant was diagnosed with severe combined immunodeficiency (SCID). A homozygous variation c.2147G>A (NM_000448.2: exonme2: c.2147G>A (p.Arg716Gln)) was identified in the RAG1 gene using whole-exome sequencing and Sanger sequencing. The predicted 3D structure of variant RAG1 indicated altered protein stability. Additionally, decreased expression of variant RAG1 gene was detected at both the mRNA and protein levels., Conclusions: Our study identified a novel homozygous variant in RAG1 gene that causes SCID. This finding expands the variant spectrum of RAG1 in SCID and provides further evidence for the clinical diagnosis of SCID., (© 2023. The Author(s).)

The objective of this study was to determine the fermentable fiber (FF) content of several common fibrous ingredients fed to nursery pigs, and then evaluate the effect of dietary FF level on growth performance and fecal microbial composition. In experiment 1, 54 nursery pigs were randomly allotted to be fed nine diets with six replicate pigs per diet. Dietary treatments included a corn-soybean meal basal diet and eight test diets based on a mixture of the corn-soybean meal diet and corn distillers dried grains with solubles, sunflower meal, oat bran, wheat bran, corn bran, sugar beet pulp (SBP), apple pomace (AP) or soybean hulls (SH). In experiment 2, 180 nursery pigs were housed in 30 pens (six pigs per pen) and randomly allotted to be fed five diets with different FF to total dietary fiber (TDF) ratios, which were 0.52, 0.55, 0.58, 0.61, and 0.64, respectively. Results showed that the FF content in SBP, AP, and SH was greater (P < 0.01) than that in other ingredients. Water binding capacity of fibrous ingredients was positively correlated (P < 0.05) to the digestibility of TDF, acid detergent fiber, and non-starch polysaccharides in test ingredients. Pigs fed the SBP, AP and SH diets had greater (P < 0.05) fecal acetic acid and total short-chain fatty acids (SCFAs) concentrations compared with pigs fed other diets. Fecal acetic acid and total SCFAs concentrations were positively correlated (P < 0.05) with FF content in experimental diets. Average daily weight gain and average daily feed intake of pigs quadratically increased (P < 0.01) as the ratios of FF to TDF increased. Pigs in FF64% group showed higher (P < 0.05) ACE index and fecal acetic acid concentration compared with pigs fed the dietary FF/TDF ratio of 0.52 to 0.61. Compared with the classification system of soluble dietary fiber and insoluble dietary fiber, FF could better describe the mechanism by which dietary fiber has beneficial effects on pig gut health., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Objective: Our goal is to determine the prognostic value of serum N-terminal prohormone of brain natriuretic peptide (NT-proBNP), leukocytosis, and hyperglycemia in patients with severe hand, foot, and mouth disease (HFMD)., Design: This is a prospective cohort study conducted from March 2011 through October 2012 at Hunan Children's Hospital., Setting: Hunan Children's Hospital, a large children's teaching hospital with 1,500-beds located in the Changsha region of Hunan Province in China., Patients: 295 children who were presented with clinical manifestation of severe HFMD, and required hospitalization., Interventions: Standard supportive treatment for HFMD as recommended by the national guidelines., Measurements: Admission blood samples were analyzed for NT-proBNP, leukocyte count, and serum glucose. Independent prognostic value of NT-proBNP for predicting mortality was evaluated using the Cox proportional hazard model adjusting for various covariates., Main Results: Area under the curve of receiver operating characteristic (AUROC) analysis suggested that a serum concentration of NT-proBNP concentration more than 1,500 pg/mL is an optimal cutoff point. Twenty-four patients (8.1%) had an NT-proBNP more than 1,500 pg/mL, and a 3-day mortality of 46% (11/24). Adjusted for tachycardia, tachypnea, hypertension, hyperglycemia, leukocytosis, and conscious disturbance on presentation, elevated NT-proBNP was associated with a 22.5-fold (95% confidence interval, 3.56-142.66) increased risk of 3-day mortality. We have further improved the specificity and AUROC values by the HFMD laboratory score, which combines NT-proBNP, leukocytosis, and hyperglycemia., Conclusions: Routine admission surveillance for NT-proBNP is useful for identifying patients with HFMD at risk for mortality. Further studies are needed to determine whether early intervention in patients with highly elevated NT-proBNP can improve outcome.