12 results on '"Wurzer, Hannah"'
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2. How natural killer cells avoid self-destruction when killing their targets.
- Author
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Wurzer H, Filali L, and Thomas C
- Subjects
- Cytotoxicity, Immunologic, Killer Cells, Natural
- Abstract
How cytotoxic lymphocytes are protected against their own weapons during close combat with diseased target cells is an important and long-standing question in immunology. A study in this issue provides new insights into the mechanisms by which natural killer (NK) cells avoid self-destruction., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2021
- Full Text
- View/download PDF
3. Intrinsic Resistance of Chronic Lymphocytic Leukemia Cells to NK Cell-Mediated Lysis Can Be Overcome In Vitro by Pharmacological Inhibition of Cdc42-Induced Actin Cytoskeleton Remodeling.
- Author
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Wurzer H, Filali L, Hoffmann C, Krecke M, Biolato AM, Mastio J, De Wilde S, François JH, Largeot A, Berchem G, Paggetti J, Moussay E, and Thomas C
- Subjects
- Actin Cytoskeleton drug effects, Biomarkers, Cell Line, Tumor, Fluorescent Antibody Technique, HLA-G Antigens immunology, Humans, Immunological Synapses immunology, Immunological Synapses metabolism, Immunophenotyping, Killer Cells, Natural drug effects, Leukemia, Lymphocytic, Chronic, B-Cell drug therapy, Leukemia, Lymphocytic, Chronic, B-Cell pathology, Tumor Microenvironment genetics, Tumor Microenvironment immunology, Actin Cytoskeleton metabolism, Cytotoxicity, Immunologic drug effects, Killer Cells, Natural immunology, Killer Cells, Natural metabolism, Leukemia, Lymphocytic, Chronic, B-Cell immunology, Leukemia, Lymphocytic, Chronic, B-Cell metabolism, cdc42 GTP-Binding Protein antagonists & inhibitors
- Abstract
Natural killer (NK) cells are innate effector lymphocytes with strong antitumor effects against hematologic malignancies such as chronic lymphocytic leukemia (CLL). However, NK cells fail to control CLL progression on the long term. For effective lysis of their targets, NK cells use a specific cell-cell interface, known as the immunological synapse (IS), whose assembly and effector function critically rely on dynamic cytoskeletal changes in NK cells. Here we explored the role of CLL cell actin cytoskeleton during NK cell attack. We found that CLL cells can undergo fast actin cytoskeleton remodeling which is characterized by a NK cell contact-induced accumulation of actin filaments at the IS. Such polarization of the actin cytoskeleton was strongly associated with resistance against NK cell-mediated cytotoxicity and reduced amounts of the cell-death inducing molecule granzyme B in target CLL cells. Selective pharmacological targeting of the key actin regulator Cdc42 abrogated the capacity of CLL cells to reorganize their actin cytoskeleton during NK cell attack, increased levels of transferred granzyme B and restored CLL cell susceptibility to NK cell cytotoxicity. This resistance mechanism was confirmed in primary CLL cells from patients. In addition, pharmacological inhibition of actin dynamics in combination with blocking antibodies increased conjugation frequency and improved CLL cell elimination by NK cells. Together our results highlight the critical role of CLL cell actin cytoskeleton in driving resistance against NK cell cytotoxicity and provide new potential therapeutic point of intervention to target CLL immune escape., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Wurzer, Filali, Hoffmann, Krecke, Biolato, Mastio, De Wilde, François, Largeot, Berchem, Paggetti, Moussay and Thomas.)
- Published
- 2021
- Full Text
- View/download PDF
4. Higher Incidence of B Cell Malignancies in Primary Immunodeficiencies: A Combination of Intrinsic Genomic Instability and Exocytosis Defects at the Immunological Synapse.
- Author
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Mastio J, Saeed MB, Wurzer H, Krecke M, Westerberg LS, and Thomas C
- Subjects
- Actin Cytoskeleton genetics, Actin Cytoskeleton immunology, B-Lymphocytes immunology, B-Lymphocytes pathology, Cell Proliferation, Cell Transformation, Neoplastic genetics, Cell Transformation, Neoplastic immunology, DNA Repair genetics, DNA Repair immunology, Exocytosis genetics, Exocytosis immunology, Genomic Instability, Humans, Immunological Synapses genetics, Leukemia, B-Cell genetics, Leukemia, B-Cell immunology, Lymphoma, B-Cell genetics, Lymphoma, B-Cell immunology, Models, Immunological, Primary Immunodeficiency Diseases genetics, Primary Immunodeficiency Diseases immunology, Risk Factors, Tumor Escape genetics, Leukemia, B-Cell etiology, Lymphoma, B-Cell etiology, Primary Immunodeficiency Diseases complications
- Abstract
Congenital defects of the immune system called primary immunodeficiency disorders (PID) describe a group of diseases characterized by a decrease, an absence, or a malfunction of at least one part of the immune system. As a result, PID patients are more prone to develop life-threatening complications, including cancer. PID currently include over 400 different disorders, however, the variety of PID-related cancers is narrow. We discuss here reasons for this clinical phenotype. Namely, PID can lead to cell intrinsic failure to control cell transformation, failure to activate tumor surveillance by cytotoxic cells or both. As the most frequent tumors seen among PID patients stem from faulty lymphocyte development leading to leukemia and lymphoma, we focus on the extensive genomic alterations needed to create the vast diversity of B and T lymphocytes with potential to recognize any pathogen and why defects in these processes lead to malignancies in the immunodeficient environment of PID patients. In the second part of the review, we discuss PID affecting tumor surveillance and especially membrane trafficking defects caused by altered exocytosis and regulation of the actin cytoskeleton. As an impairment of these membrane trafficking pathways often results in dysfunctional effector immune cells, tumor cell immune evasion is elevated in PID. By considering new anti-cancer treatment concepts, such as transfer of genetically engineered immune cells, restoration of anti-tumor immunity in PID patients could be an approach to complement standard therapies., (Copyright © 2020 Mastio, Saeed, Wurzer, Krecke, Westerberg and Thomas.)
- Published
- 2020
- Full Text
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5. Escape of tumor cells from the NK cell cytotoxic activity.
- Author
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Cantoni C, Wurzer H, Thomas C, and Vitale M
- Subjects
- Animals, Humans, Killer Cells, Natural pathology, Neoplasms pathology, Cytotoxicity, Immunologic, Killer Cells, Natural immunology, Neoplasms immunology, Tumor Escape, Tumor Microenvironment immunology
- Abstract
In recent years, NK cells, initially identified as potent cytotoxic effector cells, have revealed an unexpected complexity, both at phenotypic and functional levels. The discovery of different NK cell subsets, characterized by distinct gene expression and phenotypes, was combined with the characterization of the diverse functions NK cells can exert, not only as circulating cells, but also as cells localized or recruited in lymphoid organs and in multiple tissues. Besides the elimination of tumor and virus-infected cells, these functions include the production of cytokines and chemokines, the regulation of innate and adaptive immune cells, the influence on tissue homeostasis. In addition, NK cells display a remarkable functional plasticity, being able to adapt to the environment and to develop a kind of memory. Nevertheless, the powerful cytotoxic activity of NK cells remains one of their most relevant properties, particularly in the antitumor response. In this review, the process of tumor cell recognition and killing mediated by NK cells, starting from the generation of cytolytic granules and recognition of target cell, to the establishment of the NK cell immunological synapse, the release of cytotoxic molecules, and consequent tumor cell death is described. Next, the review focuses on the heterogeneous mechanisms, either intrinsic to tumors or induced by the tumor microenvironment, by which cancer cells can escape the NK cell-mediated attack., (©2020 Society for Leukocyte Biology.)
- Published
- 2020
- Full Text
- View/download PDF
6. Actin remodeling and vesicular trafficking at the tumor cell side of the immunological synapse direct evasion from cytotoxic lymphocytes.
- Author
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Biolato AM, Filali L, Wurzer H, Hoffmann C, Gargiulo E, Valitutti S, and Thomas C
- Subjects
- Actin Cytoskeleton pathology, Animals, Humans, Immunological Synapses pathology, T-Lymphocytes, Cytotoxic pathology, Actin Cytoskeleton immunology, Actins immunology, Immunological Synapses immunology, Neoplasm Proteins immunology, T-Lymphocytes, Cytotoxic immunology, Tumor Escape
- Published
- 2020
- Full Text
- View/download PDF
7. Actin Cytoskeleton Straddling the Immunological Synapse between Cytotoxic Lymphocytes and Cancer Cells.
- Author
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Wurzer H, Hoffmann C, Al Absi A, and Thomas C
- Subjects
- Animals, Cell Line, Humans, Immune Evasion, Killer Cells, Natural cytology, Mice, T-Lymphocytes, Cytotoxic cytology, Actin Cytoskeleton metabolism, Actin Cytoskeleton physiology, Immunological Synapses immunology, Killer Cells, Natural immunology, Neoplasms immunology, T-Lymphocytes, Cytotoxic immunology, Tumor Microenvironment immunology
- Abstract
The immune system is a fundamental part of the tumor microenvironment. In particular, cytotoxic lymphocytes, such as cytolytic T cells and natural killer cells, control tumor growth and disease progression by interacting and eliminating tumor cells. The actin cytoskeleton of cytotoxic lymphocytes engaged in an immunological synapse has received considerable research attention. It has been recognized as a central mediator of the formation and maturation of the immunological synapse, and its signaling and cytolytic activities. In comparison, fewer studies have explored the organization and function of actin filaments on the target cancer cell side of the immunological synapse. However, there is growing evidence that the actin cytoskeleton of cancer cells also undergoes extensive remodeling upon cytotoxic lymphocyte attack, and that such remodeling can alter physical and functional interactions at the immunological synapse. In this article, we review the current knowledge of actin organization and functions at both sides of the immunological synapse between cytotoxic lymphocytes and cancer cells, with particular focus on synapse formation, signaling and cytolytic activity, and immune evasion.
- Published
- 2019
- Full Text
- View/download PDF
8. Do tumor cells escape from natural killer cell cytotoxicity by mimicking dendritic cells?
- Author
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Wurzer H, Al Absi A, Hoffmann C, and Thomas C
- Published
- 2019
- Full Text
- View/download PDF
9. Actin Cytoskeleton Remodeling Drives Breast Cancer Cell Escape from Natural Killer-Mediated Cytotoxicity.
- Author
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Al Absi A, Wurzer H, Guerin C, Hoffmann C, Moreau F, Mao X, Brown-Clay J, Petrolli R, Casellas CP, Dieterle M, Thiery JP, Chouaib S, Berchem G, Janji B, and Thomas C
- Subjects
- Apoptosis, Breast Neoplasms pathology, Cell Line, Tumor, Cytotoxicity, Immunologic, Drug Resistance, Neoplasm, Female, Flow Cytometry, Humans, Leukocytes, Mononuclear cytology, MCF-7 Cells, Peptide Hydrolases metabolism, Signal Transduction, Wiskott-Aldrich Syndrome Protein, Neuronal metabolism, cdc42 GTP-Binding Protein metabolism, Actin Cytoskeleton metabolism, Breast Neoplasms metabolism, Granzymes metabolism, Killer Cells, Natural metabolism
- Abstract
Elucidation of the underlying molecular mechanisms of immune evasion in cancer is critical for the development of immunotherapies aimed to restore and stimulate effective antitumor immunity. Here, we evaluate the role of the actin cytoskeleton in breast cancer cell resistance to cytotoxic natural killer (NK) cells. A significant fraction of breast cancer cells responded to NK-cell attack via a surprisingly rapid and massive accumulation of F-actin near the immunologic synapse, a process we termed "actin response." Live-cell imaging provided direct evidence that the actin response is associated with tumor cell resistance to NK-cell-mediated cell death. High-throughput imaging flow cytometry analyses showed that breast cancer cell lines highly resistant to NK cells were significantly enriched in actin response-competent cells as compared with susceptible cell lines. The actin response was not associated with a defect in NK-cell activation but correlated with reduced intracellular levels of the cytotoxic protease granzyme B and a lower rate of apoptosis in target cells. Inhibition of the actin response by knocking down CDC42 or N-WASP led to a significant increase in granzyme B levels in target cells and was sufficient to convert resistant breast cancer cell lines into a highly susceptible phenotype. The actin response and its protective effects were fully recapitulated using donor-derived primary NK cells as effector cells. Together, these findings establish the pivotal role of actin remodeling in breast cancer cell resistance to NK-cell-mediated killing. Significance: These findings establish the pivotal role of the actin cytoskeleton in driving breast cancer cell resistance to natural killer cells, a subset of cytotoxic lymphocytes with important roles in innate antitumor immunity. Cancer Res; 78(19); 5631-43. ©2018 AACR ., (©2018 American Association for Cancer Research.)
- Published
- 2018
- Full Text
- View/download PDF
10. Constitutive activation of WASp in X-linked neutropenia renders neutrophils hyperactive.
- Author
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Keszei M, Record J, Kritikou JS, Wurzer H, Geyer C, Thiemann M, Drescher P, Brauner H, Köcher L, James J, He M, Baptista MA, Dahlberg CI, Biswas A, Lane DP, Song W, Pütsep K, Vandenberghe P, Snapper SB, and Westerberg LS
- Subjects
- Animals, Congenital Bone Marrow Failure Syndromes, Female, Gain of Function Mutation, Gene Knock-In Techniques, Humans, Male, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Transgenic, Neutropenia congenital, Neutrophils ultrastructure, Phagocytosis, Phosphorylation, Protein Conformation, Wiskott-Aldrich Syndrome Protein chemistry, Genetic Diseases, X-Linked genetics, Genetic Diseases, X-Linked metabolism, Neutropenia genetics, Neutropenia metabolism, Neutrophils metabolism, Wiskott-Aldrich Syndrome Protein genetics, Wiskott-Aldrich Syndrome Protein metabolism
- Abstract
Congenital neutropenia is characterized by low absolute neutrophil numbers in blood, leading to recurrent bacterial infections, and patients often require life-long granulocyte CSF (G-CSF) support. X-linked neutropenia (XLN) is caused by gain-of-function mutations in the actin regulator Wiskott-Aldrich syndrome protein (WASp). To understand the pathophysiology in XLN and the role of WASp in neutrophils, we here examined XLN patients and 2 XLN mouse models. XLN patients had reduced myelopoiesis and extremely low blood neutrophil number. However, their neutrophils had a hyperactive phenotype and were present in normal numbers in XLN patient saliva. Murine XLN neutrophils were hyperactivated, with increased actin dynamics and migration into tissues. We provide molecular evidence that the hyperactivity of XLN neutrophils is caused by WASp in a constitutively open conformation due to contingent phosphorylation of the critical tyrosine-293 and plasma membrane localization. This renders WASp activity less dependent on regulation by PI3K. Our data show that the amplitude of WASp activity inside a cell could be enhanced by cell-surface receptor signaling even in the context in which WASp is already in an active conformation. Moreover, these data categorize XLN as an atypical congenital neutropenia in which constitutive activation of WASp in tissue neutrophils compensates for reduced myelopoiesis.
- Published
- 2018
- Full Text
- View/download PDF
11. Wiskott-Aldrich syndrome gene mutations modulate cancer susceptibility in the p53 ± murine model.
- Author
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Keszei M, Kritikou JS, Sandfort D, He M, Oliveira MMS, Wurzer H, Kuiper RV, and Westerberg LS
- Abstract
The Wiskott-Aldrich syndrome protein (WASp) is a key regulator of the actin cytoskeleton in hematopoietic cells and mutated in two severe immunodeficiency diseases with high incidence of cancer. Wiskott-Aldrich syndrome (WAS) is caused by loss-of-function mutations in WASp and most frequently associated with lymphoreticular tumors of poor prognosis. X-linked neuropenia (XLN) is caused by gain-of-function mutations in WASp and associated with acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). To understand the role of WASp in tumorigenesis, we bred WASp
+ , WASp- , and WASp-XLN mice onto tumor susceptible p53+/- background and sub-lethally irradiated them to enhance tumor development. We followed the cohorts for 24 weeks and tumors were characterized by histology and flow cytometry to define the tumor incidence, onset, and cell origin. We found that p53+/- WASp+ mice developed malignancies, including solid tumors and T cell lymphomas with 71.4% of survival 24 weeks after irradiation. p53+/- WASp- mice showed lower survival rate and developed various early onset malignancies. Surprisingly, the p53+/- WASp-XLN mice developed malignancy mostly with late onset, which caused delayed mortality in this colony. This study provides evidence for that loss-of-function and gain-of-function mutations in WASp influence tumor incidence and onset.- Published
- 2018
- Full Text
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12. Hypoxia promotes breast cancer cell invasion through HIF-1α-mediated up-regulation of the invadopodial actin bundling protein CSRP2.
- Author
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Hoffmann C, Mao X, Brown-Clay J, Moreau F, Al Absi A, Wurzer H, Sousa B, Schmitt F, Berchem G, Janji B, and Thomas C
- Subjects
- Adult, Aged, Animals, Breast pathology, Breast Neoplasms mortality, Breast Neoplasms pathology, Cell Hypoxia, Cell Line, Tumor, Cell Movement, Female, Gene Knockdown Techniques, Humans, Hypoxia-Inducible Factor 1, alpha Subunit genetics, Kaplan-Meier Estimate, LIM Domain Proteins metabolism, Mice, Middle Aged, Muscle Proteins metabolism, Neoplasm Invasiveness pathology, Nuclear Proteins metabolism, Promoter Regions, Genetic, RNA, Small Interfering metabolism, Up-Regulation, Xenograft Model Antitumor Assays, Breast Neoplasms genetics, Extracellular Matrix pathology, Gene Expression Regulation, Neoplastic, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, LIM Domain Proteins genetics, Muscle Proteins genetics, Nuclear Proteins genetics
- Abstract
Hypoxia is a common feature of solid tumours that promotes invasion and metastatic dissemination. Invadopodia are actin-rich membrane protrusions that direct extracellular matrix proteolysis and facilitate tumour cell invasion. Here, we show that CSRP2, an invadopodial actin bundling protein, is upregulated by hypoxia in various breast cancer cell lines, as well as in pre-clinical and clinical breast tumour specimens. We functionally characterized two hypoxia responsive elements within the proximal promoter of CSRP2 gene which are targeted by hypoxia-inducible factor-1 (HIF-1) and required for promoter transactivation in response to hypoxia. Remarkably, CSRP2 knockdown significantly inhibits hypoxia-stimulated invadopodium formation, ECM degradation and invasion in MDA-MB-231 cells, while CSRP2 forced expression was sufficient to enhance the invasive capacity of HIF-1α-depleted cells under hypoxia. In MCF-7 cells, CSRP2 upregulation was required for hypoxia-induced formation of invadopodium precursors that were unable to promote ECM degradation. Collectively, our data support that CSRP2 is a novel and direct cytoskeletal target of HIF-1 which facilitates hypoxia-induced breast cancer cell invasion by promoting invadopodia formation.
- Published
- 2018
- Full Text
- View/download PDF
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