113 results on '"Wu, Yichen"'
Search Results
2. Tudor-SN exacerbates pathological vascular remodeling by promoting the polyubiquitination of PTEN via NEDD4-1.
- Author
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Wu Y, Chen Z, Zheng Z, Li X, Shu J, Mao R, An J, Fan S, Luo R, Guo Y, Xu W, Liang M, Huang K, and Wang C
- Subjects
- Animals, Mice, Muscle, Smooth, Vascular metabolism, Male, Myocytes, Smooth Muscle metabolism, Mice, Inbred C57BL, Nedd4 Ubiquitin Protein Ligases metabolism, Nedd4 Ubiquitin Protein Ligases genetics, Vascular Remodeling, PTEN Phosphohydrolase metabolism, PTEN Phosphohydrolase genetics, Ubiquitination
- Abstract
Background: Dysregulation of vascular homeostasis can induce cardiovascular diseases and increase global mortality rates. Although lineage tracing studies have confirmed the pivotal role of modulated vascular smooth muscle cells (VSMCs) in the progression of pathological vascular remodeling, the underlying mechanisms are still unclear., Methods: The expression of Tudor-SN was determined in VSMCs of artery stenosis, PDGF-BB-treated VSMCs and atherosclerotic plaque. Loss- and gain-of-function approaches were used to explore the role of Tudor-SN in the modulation of VSMCs phenotype both in vivo and in vitro., Results: In this study, we demonstrate that Tudor-SN expression is significantly elevated in injury-induced arteries, atherosclerotic plaques, and PDGF-BB-stimulated VSMCs. Tudor-SN deficiency attenuates, but overexpression aggravates the synthetic phenotypic switching of VSMCs and pathological vascular remodeling. Loss of Tudor-SN also reduces atherosclerotic plaque formation and increases plaque stability. Mechanistically, PTEN, the major regulator of the MAPK and PI3K-AKT signaling pathways, plays a vital role in Tudor-SN-mediated regulation on proliferation and migration of VSMCs. Tudor-SN facilitates the polyubiquitination and degradation of PTEN via NEDD4-1, thus exacerbating vascular remodeling under pathological conditions. BpV (HOpic), a specific inhibitor of PTEN, not only counteracts the protective effect of Tudor-SN deficiency on proliferation and migration of VSMCs, but also abrogates the negative effect of carotid artery injury-induced vascular remodeling in mice., Conclusions: Our findings reveal that Tudor-SN deficiency significantly ameliorated pathological vascular remodeling by reducing NEDD4-1-dependent PTEN polyubiquitination, suggesting that Tudor-SN may be a novel target for preventing vascular diseases., (© 2024. The Author(s).)
- Published
- 2024
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3. Stabilizing the Ferroelectric Phase of Hf_{0.5}Zr_{0.5}O_{2} Thin Films by Charge Transfer.
- Author
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Shi S, Cao T, Xi H, Niu J, Jing X, Su H, Yu X, Yang P, Wu Y, Yan X, Tian H, Tsymbal EY, and Chen J
- Abstract
Ferroelectric hafnia-based thin films have attracted significant interest due to their compatibility with complementary metal-oxide-semiconductor technology (CMOS). Achieving and stabilizing the metastable ferroelectric phase in these films is crucial for their application in ferroelectric devices. Recent research efforts have concentrated on the stabilization of the ferroelectric phase in hafnia-based films and delving into the mechanisms responsible for this stability. In this study, we experimentally demonstrate that stabilization of the ferroelectric phase in Hf_{0.5}Zr_{0.5}O_{2} (HZO) can be controlled by the interfacial charge transfer and the associated hole doping of HZO. Using the meticulously engineered charge transfer between an La_{1-x}Sr_{x}MnO_{3} buffer layer with variable Sr concentration x and an HZO film, we find the optimal x=0.33 that provides the required hole doping of HZO to most efficiently stabilize its ferroelectric phase. Our theoretical modeling reveals that the competition of the hole distribution between the threefold and fourfold coordinated oxygen sites in HZO controls the enhancement or reduction of the ferroelectric phase. Our findings offer a novel strategy to stabilize the ferroelectric phase of hafnia-based films and provide new insights into the development of ferroelectric devices compatible with CMOS.
- Published
- 2024
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4. Catalytic Kinetic Resolution of Monohydrosilanes via Rhodium-Catalyzed Enantioselective Intramolecular Hydrosilylation.
- Author
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Gou FH, Ren F, Wu Y, and Wang P
- Abstract
The catalytic access of silicon-stereogenic organosilanes remains a big challenge, and largely depends on the desymmetrization of the symmetric precursors with two identical substitutes attached to silicon atom. Here we report the construction of silicon-stereogenic organosilanes via catalytic kinetic resolution of racemic monohydrosilanes with good to excellent selectivity factors. Both Si-stereogenic dihydrobenzosiloles and Si-stereogenic monohydrosilanes could be efficiently accessed in one single operation via Rh-catalyzed enantioselective intramolecular hydrosilylation, employing (R,R)-Et-DuPhos as the optimal ligand. This catalytic protocol features mild conditions, a low catalyst loading (0.1 mol % [Rh(cod)Cl]
2 ), high stereoinduction (S factor up to 152), and excellent scalability. Moreover, further derivatizations led to the efficient synthesis of uncommon middle-size (7- and 8-membered) Si-stereogenic silacycles. Preliminary mechanistic study indicates this reaction might undergo a modified Chalk-Harrod mechanism., (© 2024 Wiley-VCH GmbH.)- Published
- 2024
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5. A Case of Palmoplantar Porokeratosis With Hypokeratosis: A New Subtype of Porokeratosis?
- Author
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Wu Y, Gao X, Yu Q, Shi J, Xu Y, and Chen J
- Abstract
Competing Interests: The authors declare no conflicts of interest.
- Published
- 2024
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6. Construction of Si-Stereogenic Silanols by Palladium-Catalyzed Enantioselective C-H Alkenylation.
- Author
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Zhao JH, Zheng L, Zou JY, Zhang SY, Shen HC, Wu Y, and Wang P
- Abstract
The construction of silicon-stereogenic silanols via Pd-catalyzed intermolecular C-H alkenylation with the assistance of a commercially available L-pyroglutamic acid has been realized for the first time. Employing oxime ether as the directing group, silicon-stereogenic silanol derivatives could be readily prepared with excellent enantioselectivities, featuring a broad substrate scope and good functional group tolerance. Moreover, parallel kinetic resolution with unsymmetric substrates further highlighted the generality of this protocol. Mechanistic studies indicate that L-pyroglutamic acid could stabilize the Pd catalyst and provide excellent chiral induction. Preliminary computational studies unveil the origin of the enantioselectivity in the C-H bond activation step., (© 2024 Wiley-VCH GmbH.)
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- 2024
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7. Pd(II)-Catalyzed 1,2-Oxyarylation of Alkenes with O -Acylhydroxylamines as the Oxygen Source.
- Author
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Wang DM, Yang L, Chen DP, Wu Y, Tang Y, and Wang P
- Abstract
O -Acylhydroxylamine has been widely employed as an electrophilic amination reagent in transition-metal-catalyzed C-N coupling reactions, but its use as an electrophilic oxygen source has not been disclosed. Here, we report a Pd-catalyzed 1,2-oxyarylation of alkenes with O -acylhydroxylamines as an oxidant and an oxygen source for the first time. With simple amide as the monodentate directing group, this method features a broad substrate scope, good functional group tolerance, and mild conditions.
- Published
- 2024
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8. Topology Prediction of Gas-Separating Metal-Organic Frameworks with Low Symmetry Vertices.
- Author
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Wu Y, Xu H, Li X, Rao Y, Yuan S, Yan Y, Zhang YB, and Li Q
- Abstract
Topology serves as a blueprint for the construction of reticular structures such as metal-organic frameworks, especially for those based on building blocks with highly symmetrical shapes. However, it remains a challenge to predict the topology of the frameworks from less symmetrical units, because their corresponding vertex figures are largely deformed from the perfect geometries with no "default" net embedding. Furthermore, vertices involving flexible units may have multiple shape choices, and the competition among their designated topologies makes the structure prediction in large uncertainty. Herein, the deformation index is proposed to characterize the symmetry loss of the vertex figure by comparing it with its ideal geometry. The mathematical index is employed to predict the shapes of two in situ formed Co-based metalloligands (pseudo-tetrahedron and pseudo-square), which further dictate the framework topology (flu and scu) when they are joined with the [Zr
6 O8 ]-based cuboid units. The two frameworks with very similar constituents provide an ideal platform to investigate how the pore shapes and interconnectivity influence the gas separation. The net with cylindrical channels outperforms the other with discreate cages in C3 H8 /C2 H6 /CH4 separation, benefiting from the facile accessibility of its interaction sites to the guests imposed by the specific framework topology., (© 2024 Wiley‐VCH GmbH.)- Published
- 2024
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9. Recovery mechanism of bio-promoters on Cr(VI) suppressed denitrification: Toxicity remediation and enhanced electron transmission.
- Author
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Wang Q, Zhao Y, Liu Y, Zhang C, Bamanu B, Wu Y, Chao C, Liu Y, Tong Y, and Nuramkhaan M
- Subjects
- Electrons, Chromium toxicity, Chromium chemistry, Denitrification, Metals, Heavy
- Abstract
Although the biotoxicity of heavy metals has been widely studied, there are few reports on the recovery strategy of the inhibited bio-system. This study proposed a combined promoter-I (Primary promoter: l-cysteine, biotin, and cytokinin + Electron-shuttle: PMo
12 ) to recover the denitrification suppressed by Cr(VI). Compared with self-recovery, combined promoter-I shortened the recovery time of 28 cycles, and the recovered reactor possessed more stable long-term operation performance with >95 % nitrogen removal. The biomass increased by 7.07 mg VSS/(cm3 carrier) than self-recovery due to the promoted bacterial reproduction, thereby reducing the toxicity load of chromium per unit biomass. The combined promoter-I strengthened the toxicity remediation by promoting 92.84 % of the intracellular chromium release and rapidly activating anti-oxidative stress response. During toxicity remediation, ROS content quickly decreased, and the PN/PS value was 2.27 times that of self-recovery. PMo12 relieved Cr(VI) inhibition on NO3 - -N reduction by increasing NAR activity. The enhanced intracellular and intercellular electron transmission benefited from the stimulated NADH, FMN, and Cyt.c secretion by the primary promoter and the improved transmembrane electron transmission by Mo. PMo12 and the primary promoter synergized in regulating community structure and improving microbial richness. This study provided practical approaches for microbial toxicity remediation and maintaining high-efficiency denitrification., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)- Published
- 2024
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10. Versatile photonic molecule switch in multimode microresonators.
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Tao Z, Shen B, Li W, Xing L, Wang H, Wu Y, Tao Y, Zhou Y, He Y, Peng C, Shu H, and Wang X
- Abstract
Harnessing optical supermode interaction to construct artificial photonic molecules has uncovered a series of fundamental optical phenomena analogous to atomic physics. Previously, the distinct energy levels and interactions in such two-level systems were provided by coupled microresonators. The reconfigurability is limited, as they often require delicate external field stimuli or mechanically altering the geometric factors. These highly specific approaches also limit potential applications. Here, we propose a versatile on-chip photonic molecule in a multimode microring, utilizing a flexible regulation methodology to dynamically control the existence and interaction strength of spatial modes. The transition between single/multi-mode states enables the "switched-off/on" functionality of the photonic molecule, supporting wider generalized applications scenarios. In particular, "switched-on" state shows flexible and multidimensional mode splitting control in aspects of both coupling strength and phase difference, equivalent to the a.c. and d.c. Stark effect. "Switched-off" state allows for perfect low-loss single-mode transition (Q
i ~ 10 million) under an ultra-compact bend size (FSR ~ 115 GHz) in a foundry-based silicon microring. It breaks the stereotyped image of the FSR-Q factor trade-off, enabling ultra-wideband and high-resolution millimeter-wave photonic operations. Our demonstration provides a flexible and portable solution for the integrated photonic molecule system, extending its research scope from fundamental physics to real-world applications such as nonlinear optical signal processing and sixth-generation wireless communication., (© 2024. The Author(s).)- Published
- 2024
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11. Stepwise construction of coordinative linkages and dynamic covalent linkages for a porous metal-organic framework.
- Author
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Peng S, Sun Y, Li Q, Jiang Z, Rao Y, Wu Y, and Li Q
- Abstract
A cyclic trinuclear complex is synthesized from Ag
I and 1 H -pyrazole-4-carbaldehyde. Reticulation of the complex with 1,3,5-tris(4-aminophenyl)benzene through Schiff-base reaction affords a porous FDM-72 framework. Amine choice is systematically investigated as it may initiate metal reduction. This study proposes a new route and its amine selection criterion to synthesize Ag-based frameworks.- Published
- 2024
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12. Catch and release: Gold adsorption and sorbent electrochemical regeneration.
- Author
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Ganzoury MA, Hanna CM, Zhang N, Wu Y, and de Lannoy CF
- Abstract
Wastewater generated from e-waste leaching is rich in precious metals including gold, silver and platinum. Conventional precipitation and solvent extraction are chemically intensive separations with concerning environmental externalities. Sorbents, in particular carbon nanotubes, have low chemical consumption, and have shown promise for gold adsorption due to their high specific surface area and chemical functionalization potential. However, regenerating sorbents used to adsorb Au is hazardous requiring strong acids. Herein, we delineate the effect of various functional groups on the sidewalls of multiwall carbon nanotubes (MWCNTs) on gold adsorption, and we introduced an acid-free electrochemical technique for Au elution from MWCNTs. Pristine MWCNTs (P-MWCNTs), carboxylic functionalised MWCNTs (COOH-MWCNTs) and amide functionalised MWCNTs (NH
2 -MWCNTs) were compared for their affinity for Au adsorption from acidic AuCl4 - solutions mimicking acidic e-waste leachate. Au adsorption affinity onto MWCNTs followed the order of P-MWCNT > NH2 -MWCNT > COOH-MWCNTs. Au elution from Au-saturated MWCNTs was subsequently achieved up to 65%, using acid-free electrochemical desorption in neutral aqueous brine. The Au electro-desorption was shown to have a direct relationship with both the applied current and the mass of the Au adsorbed on the MWCNTs. This study demonstrates enhanced adsorption-based preconcentration of gold and acid-free regeneration of sorbents., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2024
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13. Diphosphine Ligand-Enabled Nickel-Catalyzed Chelate-Assisted Inner-Selective Migratory Hydroarylation of Alkenes.
- Author
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He HD, Chitrakar R, Cao ZW, Wang DM, She LQ, Zhao PG, Wu Y, Xu YQ, Cao ZY, and Wang P
- Abstract
The precise control of the regioselectivity in the transition metal-catalyzed migratory hydrofunctionalization of alkenes remains a big challenge. With a transient ketimine directing group, the nickel-catalyzed migratory β-selective hydroarylation and hydroalkenylation of alkenyl ketones has been realized with aryl boronic acids using alkyl halide as the mild hydride source for the first time. The key to this success is the use of a diphosphine ligand, which is capable of the generation of a Ni(II)-H species in the presence of alkyl bromide, and enabling the efficient migratory insertion of alkene into Ni(II)-H species and the sequent rapid chain walking process. The present approach diminishes organosilanes reductant, tolerates a wide array of complex functionalities with excellent regioselective control. Moreover, this catalytic system could also be applied to the migratory hydroarylation of alkenyl azahetereoarenes, thus providing a general approach for the preparation of 1,2-aryl heteroaryl motifs with wide potential applications in pharmaceutical discovery., (© 2023 Wiley-VCH GmbH.)
- Published
- 2024
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14. Phosphomolybdic acid enhancing hexavalent chromium bio-reduction in long-term operation: Optimal dosage and mechanism analysis.
- Author
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Wu Y, Zhao Y, Jia X, Liu Y, and Niu J
- Subjects
- Electron Transport, Chromium metabolism, Oxidative Stress
- Abstract
The bio-reduction of Cr(VI) is regarded as a feasible and safe strategy to treat Cr pollution. The optimal concentration of phosphomolybdic acid (PMo
12 ) for Cr(VI) reduction and the catalytic mechanism of electron behavior (electron production, electron transport and electron consumption) were revealed in denitrifying biofilm systems. The results showed that 0.1 mM PMo12 could achieve 92.5 % removal efficiency of 90 mg/L Cr(VI), which was 47.7 % higher than that of PMo12 -free system, and improve the extracellular fixation capacity of Cr(III). The activity of peroxidase (POD) was significantly promoted by PMo12 to repair oxidative stress damage caused by Cr(VI) reduction. Additionally, analysis of electron behavior demonstrated that PMo12 could enhance key indicators of electron production, transport and consumption. This led to rapid activation of the electron pathway inhibited by Cr(VI), enabling simultaneous efficient nitrogen removal and Cr(VI) reduction in the biofilm system. This discovery will provide an efficient technique for Cr-containing wastewater treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)- Published
- 2024
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15. Polydopamine and calcium functionalized fiber carrier for enhancing microbial attachment and Cr(VI) resistance.
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Wu Y, Niu J, Yuan X, Liu Y, Zhai S, and Zhao Y
- Abstract
The formation of biofilm determines the performance and stability of biofilm system. Increasing the hydrophilicity of the carrier surface could efficiently accelerate the attachment and growth of microorganisms. Here, the surface of polypropylene (PP) fiber carrier was modified with polydopamine (PDA) and calcium (Ca(II)) to enhance microbial attachment and toxicity resistance. The results of surface characteristic confirmed the self-polymerization of PDA and the chelation mechanism of Ca(II). Subsequently, the biofilm formation experiments were conducted in sequencing batch biofilm reactors using both normal and chromium-containing wastewater. The biofilm on the surface of the modified carrier exhibited better nitrogen removal and Cr(VI) reduction ability. The biomass of the modified carrier was significantly increased, and the maximum microbial attachment amounts in normal wastewater and chrome-containing wastewater were 1153.34 and 511.78 mg/g carrier, respectively. Furthermore, the confocal laser scanning microscope (CLSM) indicated that the modified carrier coated with PDA and Ca(II) were both biocompatible, and the cell activity was significantly increased. 16S rRNA sequencing results showed that the modified carrier efficiently enriched both denitrification bacteria (Thauera and Flavobacterium) and chrome-reducing bacteria (Simplicispira and Arenimonas) to improve system stability and Cr(VI) resistance. Microbial phenotype prediction based on BugBase analysis further verified the enrichment effect of modified carriers on microorganisms responsible for biofilm formation and oxidative stress resistance. Overall, this work proposed a novel functional carrier that could provide references for advancing the application of biofilm systems in wastewater treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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16. Target Design of Multinary Metal-Organic Frameworks for Near-Infrared Imaging and Chemodynamic Therapy.
- Author
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Yang L, Lu M, Wu Y, Jiang Z, Chen ZH, Tang Y, and Li Q
- Subjects
- Humans, HeLa Cells, Reactive Oxygen Species, Hydrogen Peroxide, Phototherapy, Cell Line, Tumor, Metal-Organic Frameworks pharmacology, Metal-Organic Frameworks chemistry, Nanoparticles chemistry, Neoplasms diagnostic imaging, Neoplasms drug therapy
- Abstract
Imaging-guided chemodynamic therapy is widely considered a promising modality for personalized and precision cancer treatment. Combining both imaging and chemodynamic functions in one system conventionally relies on the hybrid materials approach. However, the heterogeneous, ill-defined, and dissociative/disintegrative nature of the composites tends to complicate their action proceedings in biological environments and thus makes the treatment imprecise and ineffective. Herein, a strategy to employ two kinds of inorganic units with different functions─reactive oxygen species generation and characteristic emission─has achieved two single-crystalline metal-organic frameworks (MOFs), demonstrating the competency of reticular chemistry in creating multifunctional materials with atomic precision. The multinary MOFs could not only catalyze the transformation from H
2 O2 to hydroxyl radicals by utilizing the redox-active Cu-based units but also emit characteristic tissue-penetrating near-infrared luminescence brought by the Yb4 clusters in the scaffolds. Dual functions of MOF nanoparticles are further evidenced by pronounced cell imaging signals, elevated intracellular reactive oxygen species levels, significant cell apoptosis, and reduced cell viabilities when they are taken up by the HeLa cells. In vivo NIR imaging is demonstrated after the MOF nanoparticles are further functionalized. The independent yet interconnected modules in the intact MOFs could operate concurrently at the same cellular site, achieving a high spatiotemporal consistency. Overall, our work suggests a new method to effectively accommodate both imaging and therapy functions in one well-defined material for precise treatment.- Published
- 2023
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17. Controllable and biocompatible 3D bioprinting technology for microorganisms: Fundamental, environmental applications and challenges.
- Author
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Zhao T, Liu Y, Wu Y, Zhao M, and Zhao Y
- Subjects
- Humans, Tissue Engineering, Printing, Three-Dimensional, Biofilms, Catalysis, Biocompatible Materials, Tissue Scaffolds, Bioprinting methods
- Abstract
3D bioprinting is a new 3D manufacturing technology, that can be used to accurately distribute and load microorganisms to form microbial active materials with multiple complex functions. Based on the 3D printing of human cells in tissue engineering, 3D bioprinting technology has been developed. Although 3D bioprinting technology is still immature, it shows great potential in the environmental field. Due to the precise programming control and multi-printing pathway, 3D bioprinting technology provides a high-throughput method based on micron-level patterning for a wide range of environmental microbiological engineering applications, which makes it an on-demand, multi-functional manufacturing technology. To date, 3D bioprinting technology has been employed in microbial fuel cells, biofilm material preparation, microbial catalysts and 4D bioprinting with time dimension functions. Nevertheless, current 3D bioprinting technology faces technical challenges in improving the mechanical properties of materials, developing specific bioinks to adapt to different strains, and exploring 4D bioprinting for intelligent applications. Hence, this review systematically analyzes the basic technical principles of 3D bioprinting, bioinks materials and their applications in the environmental field, and proposes the challenges and future prospects of 3D bioprinting in the environmental field. Combined with the current development of microbial enhancement technology in the environmental field, 3D bioprinting will be developed into an enabling platform for multifunctional microorganisms and facilitate greater control of in situ directional reactions., Competing Interests: Declaration of Competing Interest No potential conflict of interest was reported by the authors., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
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18. Adult tiger beetles Cicindela gemmata modify their foraging strategy in different hunting contexts.
- Author
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Wang S, Fei P, Wu Z, Luo Z, Wu Y, Sun N, Wang Y, Zi S, Gao L, and Du B
- Subjects
- Animals, Behavior, Animal, Coleoptera, Predatory Behavior physiology
- Abstract
Predator-prey interaction has long been an interesting item in the research of animal behaviors. Given that live prey can damage their predators, predators must trade foraging efficiency for safety while hunting, but the extent of this trade-off is not yet clear. Tiger beetles display diversity in their diets and hunting strategies, and hence, they become an ideal system to address how self-security affects foraging efficiency. We addressed this question in captive adult tiger beetles Cicindela gemmata. By offering several types of arthropod and plant foods, we confirmed that C. gemmata is carnivorous. We found that C. gemmata hunt by either ambushing or chasing their prey, and that they switch between strategies based on differences in the number of prey, the prey status and encounter rate, and the number of predators. Ambushing success increased with the number of prey but decreased with prey encounter rate. Chasing success decreased as prey body size and encounter rate increased. Foraging Cicindela gemmata often gave up an attack when it was nonfatal. This active giving up of hunting may be a consequence of a trade-off between foraging efficiency and self-security. Therefore, it is an adaptive response to the risk of injury when hunting for larger live prey., (© 2023 Institute of Zoology, Chinese Academy of Sciences.)
- Published
- 2023
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19. Comparison of efficacy of non-pharmacological intervention for post-stroke dysphagia: a systematic review and Bayesian network meta-analysis.
- Author
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Zhu H, Deng X, Luan G, Zhang Y, and Wu Y
- Subjects
- Humans, Quality of Life, Prospective Studies, Bayes Theorem, Network Meta-Analysis, Treatment Outcome, Randomized Controlled Trials as Topic, Deglutition Disorders etiology, Deglutition Disorders therapy, Stroke complications, Stroke therapy, Acupuncture Therapy methods
- Abstract
Increasingly, non-pharmacological interventions are being identified and applied to post-stroke dysphagia. Nevertheless, there is insufficient evidence to assess which type of interventions are more effective. In this study, the randomized controlled trials of non-pharmacological interventions on post-stroke dysphagia were retrieved from the relevant databases. Including 96 studies and 12 non-drug treatments. Then, and the network meta-analysis is carried out by statistical software. The results show: In the aspects of videofluoroscopic swallowing study (VFSS), Standardized Swallowing Assessment (SSA), swallowing-quality of life (SWAL-QOL), Water swallow test (WST); Acupuncture + electrotherapy + rehabilitation training, acupuncture + rehabilitation training + massage, electrotherapy + rehabilitation training, acupuncture + electrotherapy + rehabilitation training, electrotherapy, acupuncture + rehabilitation training + acupoints sticking application have significant effects in post-stroke dysphagia. Compared with other interventions, they have more advantages in improving the above indicators. A substantial number of high-quality randomized clinical trials are still necessary in the prospective to validate the therapeutic effectiveness of non-pharmacological interventions in post-stroke dysphagia and the results of this Bayesian network meta-analysis., (© 2023. BioMed Central Ltd., part of Springer Nature.)
- Published
- 2023
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20. Demographic and clinical feature disparity between progress and non-progress patients with vitiligo after COVID-19 vaccination: A cross-sectional study.
- Author
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Hou X, Wu N, Xu M, Kharel P, Wu F, Wu Y, Wang R, and Chen J
- Subjects
- Adult, Aged, Female, Humans, Male, COVID-19 Vaccines adverse effects, Cross-Sectional Studies, Demography, Vaccination adverse effects, Middle Aged, COVID-19 complications, COVID-19 epidemiology, COVID-19 prevention & control, Vitiligo epidemiology, Vitiligo etiology
- Abstract
There have been several case reports regarding newly developed vitiligo following the coronavirus disease 19 (COVID-19) vaccination. However, the relationship between COVID-19 vaccine and vitiligo progression remains unclear. To explore the relationship between COVID-19 vaccine and vitiligo progression and its potential influencing factors, A cross-sectional study was conducted on 90 patients with vitiligo who received inactivated COVID-19 vaccination. Detailed information covering demographic characteristics (age and sex), vitiligo clinical features (disease subtypes, duration, stage and comorbidities) and disease activity was collected through an electronic questionnaire. Ninety patients with vitiligo included 44.4% males, with an average age of 38.1 years (standard deviation, SD = 15.0). Patients were divided into progress group (29, 32.2%) and normal group (61, 67.8%) based on whether they experienced vitiligo progression after inactivated COVID-19 vaccination. 41.3% of patients in the progress group experienced vitiligo progression within 1 week after vaccination, and disease progression mainly occurred after the first dose inoculation (20, 69.0%). Logistic regression revealed that patients aged <45 years (odds ratio (OR) was 0.87, 95% confidence interval (CI): 0.34-2.22) and male patients (OR = 0.84, 95% CI: 0.34-2.05) had lower risk for vitiligo progression, while patients with segmental vitiligo (SV) subtype (OR = 1.68, 95% CI: 0.53-5.33), with <5 years disease duration (OR = 1.32, 95% CI: 0.51-3.47) had higher risk for vitiligo progression after COVID-19 vaccination, but without statistical significance. Over 30% patients experienced vitiligo progression after inactivated COVID-19 vaccination, and female patients, elder age, shorter disease duration and SV subtype are potential risk factors for vitiligo progression., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2023
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21. Ligand-Enabled Ni II -Catalyzed Hydroxylarylation of Alkenes with Molecular Oxygen.
- Author
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Wang DM, She LQ, Yuan H, Wu Y, Tang Y, and Wang P
- Abstract
The use of molecular oxygen as the terminal oxidant in transition metal catalyzed oxidative process is an appealing and challenging task in organic synthetic chemistry. Here, we report a Ni-catalyzed hydroxylarylation of unactivated alkenes enabled by a β-diketone ligand with high efficiency and excellent regioselectivity employing molecular oxygen as the oxidant and hydroxyl source. This reaction features mild conditions, broad substrate scope and incredible heterocycle compatibility, providing a variety of β-hydroxylamides, γ-hydroxylamides, β-aminoalcohols, γ-aminoalcohols, and 1,3-diols in high yields. The synthetic value of this methodology was demonstrated by the efficient synthesis of two bioactive compounds, (±)-3'-methoxyl citreochlorol and tea catechin metabolites M4., (© 2023 Wiley-VCH GmbH.)
- Published
- 2023
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22. Generation of Site-Selective Structural Vacancies in a Multinary Metal-Organic Framework for Enhanced Catalysis.
- Author
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Wang J, Rao Y, Wu Y, Yang L, and Li Q
- Abstract
Generating structural vacancies in metal-organic frameworks (MOFs) by partially removing the inorganic and organic units from the scaffolds is an effective strategy to modulate the pore parameters of the extended structures. However, pore enlargement is accomplished at the cost of loss in the number of active sites in typical MOFs, since dissociations of coordination linkages to create vacancies are not site-selective. Here, we performed site-specific vacancy generation in a multinary MOF (FDM-6) by selectively hydrolyzing the weak Zn─carboxylate linkages and keeping the strong Cu─pyrazolate linkages untouched. Surface area and pore size range of the materials could be systematically tuned by adjusting the water content and hydrolysis time. More than 56% of the Zn(II) sites in FDM-6 could be vacant, as evidenced by the atom occupancy analysis using powder X-ray diffraction, while most of the redox-active Cu sites are held in the backbone. The vacancies create highly connected mesopores, thus guaranteeing facile transportation of the guest molecules toward the active sites. Compared with the pristine MOF, FDM-6 with site-selective vacancies shows enhanced catalytic activity in bulky aromatic alcohol oxidation. Overall, the multinary MOF provides a platform in which both pore size enhancement and full retainment of active sites could be delivered in one framework by simple vacancy engineering.
- Published
- 2023
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23. Insights into heavy metals shock on anammox systems: Cell structure-based mechanisms and new challenges.
- Author
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Wu Y, Zhao Y, Liu Y, Niu J, Zhao T, Bai X, Hussain A, and Li YY
- Subjects
- Wastewater, Oxidation-Reduction, Anaerobic Ammonia Oxidation, Anaerobiosis, Nitrogen metabolism, Bioreactors, Denitrification, Sewage chemistry, Metals, Heavy, Ammonium Compounds metabolism
- Abstract
Anaerobic ammonium oxidation (anammox) as a low-carbon and energy-saving technology, has shown unique advantages in the treatment of high ammonia wastewater. However, wastewater usually contains complex heavy metals (HMs), which pose a potential risk to the stable operation of the anammox system. This review systematically re-evaluates the HMs toxicity level from the inhibition effects and the inhibition recovery process, which can provide a new reference for engineering. From the perspective of anammox cell structure (extracellular, anammoxosome membrane, anammoxosome), the mechanism of HMs effects on cellular substances and metabolism is expounded. Furthermore, the challenges and research gaps for HMs inhibition in anammox research are also discussed. The clarification of material flow, energy flow and community succession under HMs shock will help further reveal the inhibition mechanism. The development of new recovery strategies such as bio-accelerators and bio-augmentation is conductive to breaking through the engineered limitations of HMs on anammox. This review provides a new perspective on the recognition of toxicity and mechanism of HMs in the anammox process, as well as the promotion of engineering applicability., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)
- Published
- 2023
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24. SPSiPs, a Class of Diphosphine Ligands Based on SPSiOL with a Large Dihedral Angle.
- Author
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Li H, Zhao PG, Wang CY, Zhang RY, Li JJ, Wu Y, and Wang P
- Abstract
The development and the synthetic applications of a novel class of diphosphine ligands (SPSiPs) based on chiral spirosilabiindane diol (SPSiOL) are presented. Starting from SPSiOL, the diphosphine ligands could be readily prepared in three steps with high efficiency. This novel class of diphosphine ligands features rigid configuration, a large dihedral angle, a large P-M-P angle, and a long P-P distance. The potentials of SPSiPs in asymmetric catalysis have also been preliminarily disclosed.
- Published
- 2023
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25. Cu-Mediated Thianthrenation and Phenoxathiination of Arylborons.
- Author
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Chen XY, Li YN, Wu Y, Bai J, Guo Y, and Wang P
- Abstract
Great success in synthetic chemistry is motivated by the development of novel and reactive linchpins for carbon-carbon and carbon-heteroatom bond formation reactions, which has dramatically altered chemists' approach to building molecules. Herein, we report the ready synthesis of aryl sulfonium salts, a versatile electrophilic linchpin, via a novel Cu-mediated thianthrenation and phenoxathiination of commercially available arylborons with thianthrene and phenoxathiine, providing a series of aryl sulfonium salts in high efficiency. More importantly, by leveraging the sequential Ir-catalyzed C-H borylation and Cu-mediated thianthrenation of arylborons, the formal thianthrenation of arenes is also achieved. The Ir-catalyzed C-H borylation with undirected arenes normally occurred at the less steric hindrance position, thus providing a complementary method for thianthrenation of arenes in comparison with electrophilic thianthrenation. This process is capable of late-stage functionalization of a series of pharmaceuticals, which might find wide synthetic applications in both industry and academic sectors.
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- 2023
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26. Description of a Novel Web-Based Liposuction System to Estimate Fat Volume and Distribution.
- Author
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Harutyunyan R, Gilardino MS, Wu Y, Jeffries SD, and Hemmerling TM
- Subjects
- Humans, Subcutaneous Fat diagnostic imaging, Subcutaneous Fat surgery, Body Fat Distribution, Software, Internet, Lipectomy methods
- Abstract
Background: Preoperative planning for liposuction is vital to ensure safe practice and patient satisfaction. However, current standards of fat assessment before surgery are guided by subjective methods such as visual inspection, skin-pinch tests, and waist circumference measurements., Objectives: This study aimed to develop an inexpensive software-based tool that utilizes ultrasound (US) imaging and an online platform to accurately simulate regional subcutaneous adipose tissue (SAT) distribution and safe volume estimation for liposuction procedures., Methods: The authors present a web-based platform with integrated 2-dimensional (2D) and 3-dimensional (3D) simulations of SAT to support liposuction planning and execution. SAT-Map was constructed using multiple sub-applications linked with the python framework programming language (Wilmington, DE)., Results: The SAT-Map interface provides an intuitive and fluid means of generating patient-specific models and volumetric data. To further accommodate this, an operational manual was prepared to achieve consistent visualization and examination of estimated SAT content. The system currently supports static 2D heatmap simulation and 3D interactive virtual modelling of the SAT distribution. Supplementary clinical studies are needed to evaluate SAT-Map's clinical performance and practicality., Conclusions: SAT-Map revolutionizes the concept of preoperative planning for liposuction by developing the first combined web-based software that objectively simulates fat distribution and measures safe liposuction volume. Our software approach presents a cost-efficient, accessible, and user-friendly system offering multiple advantages over current SAT assessment modalities. The immediacy of clinically accurate 3D virtual simulation provides objective support to surgeons towards improving patient conversation, outcomes, and satisfaction in liposuction procedures., (© The Author(s) 2022. Published by Oxford University Press on behalf of The Aesthetic Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2023
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27. Modeling of the Rating of Perceived Exertion Based on Heart Rate Using Machine Learning Methods.
- Author
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Zhao H, Xu Y, Wu Y, Ma Z, Ding Z, and Sun Y
- Subjects
- Humans, Heart Rate physiology, Linear Models, Machine Learning, Physical Exertion physiology, Exercise physiology
- Abstract
Rating of perceived exertion (RPE) can serve as a more convenient and economical alternative to heart rate (HR) for exercise intensity control. This study aims to explore the influence of factors, such as indicators of demographic, anthropometric, body composition, cardiovascular function and basic exercise ability on the relationship between HR and RPE, and to develop the model predicting RPE from HR. 48 healthy participants were recruited to perform an incrementally 6-stage pedaling test. HR and RPE were collected during each stage. The influencing factors were identified with the forward selection method to train Gaussian Process regression (GPR), support vector machine (SVM) and linear regression models. Metrics of R2, adjusted R2 and RMSE were calculated to evaluate the performance of the models. The GPR model outperformed the SVM and linear regression models, and achieved an R2 of 0.95, adjusted R2 of 0.89 and RMSE of 0.52. Indicators of age, resting heart rate (RHR), Central arterial pressure (CAP), body fat rate (BFR) and body mass index (BMI) were identified as factors that best predicted the relationship between RPE and HR. It is possible to use GPR model to estimate RPE from HR accurately, after adjusting for age, RHR, CAP, BFR and BMI.
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- 2023
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28. Diverse Synthesis of Alkenylsilanes via Pd-Catalyzed Alkenyl C-H Silylation.
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Ye ZH, Gou FH, Wu Y, Li CY, and Wang P
- Abstract
Here, we disclose a general approach for the diverse synthesis of alkenylsilanes in a highly efficient, stereoselective, and atom-economic manner by leveraging the palladium-catalyzed disilylation reaction of 2-bromostyrene derivatives with hexamethyldisilane, which is suitable for the preparation of a series of disubstituted, trisubstituted, and tetrasubstituted alkenylsilanes. Furthermore, the resulting tetrasubstituted alkenylsilanes could be readily transformed into the corresponding diarylated benzosiloles, which have been proven to be a potential AIE material and a fluorene material.
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- 2023
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29. PRAME Promotes Cervical Cancer Proliferation and Migration via Wnt/β-Catenin Pathway Regulation.
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Chen X, Jiang M, Zhou S, Chen H, Song G, Wu Y, and Zhu X
- Abstract
A significant burden is placed on the lives of females due to cervical cancer, which is currently the leading cause of cancer death among women. Preferentially expressed antigen in melanoma (PRAME) belongs to the CTA gene family and was found to be abnormally expressed among different types of cancers. Our previous research also indicated that PRAME was highly expressed in cervical cancer compared with normal tissues. However, the roles and detailed mechanisms of PRAME have not been explored in cervical cancer. In the present study, the expression of PRAME in cervical tissues and cells was detected by immunohistochemistry (IHC), qRT-PCR, and Western blotting. Additionally, CCK-8, BrdU, scratch, transwell, and flow cytometry assays were conducted to explore the function of PRAME in regulating the malignant biological behaviors of cervical cancer cells. Nude mice were used to confirm the role of PRAME in tumor growth in vivo. Furthermore, the Wnt inhibitor MSAB was used to verify the role of PRAME in regulating the Wnt/β-catenin pathway both in vitro and in vivo. The results of IHC, qRT-PCR, and Western blotting showed that PRAME was highly expressed in cervical cancer tissues and cells. PRAME knockdown attenuated cell growth, migration, and invasion; induced G0/G1 arrest; and increased cell apoptosis in C33A and SiHa cells through Wnt/β-catenin signaling regulation. However, the upregulation of PRAME exhibited the opposite effects accordingly, which could be partly reversed via MSAB treatment. The growth rate of xenograft tumors was enhanced when PRAME was overexpressed via Wnt/β-catenin signaling activation. Taken together, PRAME is associated with cervical cancer occurrence and progression mediated by Wnt/β-catenin signaling, suggesting that PRAME might be a factor in manipulating cervical carcinogenesis and a potential therapeutic target.
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- 2023
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30. Enantioselective Nickel-Catalyzed Hydrosilylation of 1,1-Disubstituted Allenes.
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Liu T, Mao XR, Song S, Chen ZY, Wu Y, Xu LP, and Wang P
- Abstract
Here, we report the first example of Ni-catalyzed asymmetric hydrosilylation of 1,1-disubstituted allenes with high level of regioselectivities and enantioselectivities. The key to achieve this stereoselective hydrosilylation reaction was the development of the SPSiOL-derived bisphosphite ligands (SPSiPO). This protocol features broad substrate scope, excellent functional group, and heterocycle tolerance, thus provides a versatile method for the construction of enantioenriched tertiary allylsilanes in a straightforward and atom-economic manner. DFT calculations were performed to reveal the reaction mechanism and the origins of the enantioselectivity., (© 2023 Wiley-VCH GmbH.)
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- 2023
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31. Differentiated embryonic chondrocyte expressed gene-1 is a central signaling component in the development of collagen-induced rheumatoid arthritis.
- Author
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Wu Y, Wang H, Huo Y, Yan B, Honda H, Liu W, and Yang J
- Subjects
- Animals, Humans, Mice, Collagen, Cytokines metabolism, Fibroblasts metabolism, Hyperplasia pathology, Inflammation pathology, Synovial Membrane pathology, Arthritis, Experimental chemically induced, Arthritis, Experimental genetics, Arthritis, Rheumatoid genetics, Basic Helix-Loop-Helix Transcription Factors metabolism, Homeodomain Proteins metabolism
- Abstract
Rheumatoid arthritis (RA) is one of the most common autoimmune diseases and affects almost 1% of the population. Differentiated embryo-chondrocyte expressed gene-1 (DEC1) has been associated with both osteogenesis and osteoclastogenesis. RA condition is marked by inflammatory hyperplasia, and DEC1 is known to support inflammatory reactions and implicated in antiapoptosis and cell invasion. Here, our goal was to test the hypothesis that DEC1 enhances RA development induced by collagen-induced arthritis (CIA), a well-recognized protocol for developing RA animal models. DEC1
+/+ and DEC1-/- mice were subjected to CIA protocol, and the development of RA condition was monitored. We found that CIA robustly induced RA phenotypes (e.g., synovial hyperplasia) and greatly increased the expression of proinflammatory cytokines such as TNF-α. However, these changes were detected in DEC1+/+ but not DEC1-/- mice. Interestingly, these very cytokines strongly induced DEC1, and such a dual role of DEC1, as an inducer for and being induced by proinflammatory cytokines, constitutes a DEC1-amplifying circuit for inflammation. Knockdown of DEC1 in human MH7A cells strongly decreased cell migration and invasion as well as the expression of genes related to RA phenotypes. The combination of DEC1-directed migration and invasion in vitro with synovial hyperplasia in vivo mechanistically establishes cellular bases on how DEC1 is involved in the development of RA phenotypes. In addition to inflammatory signaling, DEC1 functionally interacted with PI3KCA(p110α)/Akt/GSK3β, Wnt/β-catenin, and NFATc1. Such engagement in multiple signaling pathways suggests that DEC1 plays coordinated and integral roles in developing RA, one of the most common autoimmune diseases., Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2023
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32. PMo 12 as a redox mediator for bio-reduction of Cr(VI): Promotor or inhibitor?
- Author
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Zhao Y, Jia X, Wang Q, Wu Y, Jia Z, Zhou X, and Ji M
- Subjects
- Oxidation-Reduction, Electron Transport, Chromium toxicity
- Abstract
Slow reduction rate and low reduction ability were the main limitations of bio-reduction of Cr(VI). As an efficient redox mediator, how phosphomolybdic acid (PMo
12 ) affected bio-reduction of Cr(VI) was worthy of exploration. In this study, short-term and long-term effects of PMo12 on Cr(VI) reduction were investigated to reveal the relevant mechanism. After evaluating the short-term effect of PMo12 concentration from 0.05 to 1.00 mM on Cr(VI) bio-reduction, 0.50 mM was found to be optimum by improving Cr(VI) reduction rate by 16.3 % and microbial electron transport system activity (ETSA) by 43.0 % with Cr(VI) reduction efficiency of 100 % in short-term (22 h) batch experiments. By contrast, in long-term (28 days) continuous flow experiments, 0.50 mM PMo12 exhibited serious inhibition on Cr(VI) bio-reduction. The cumulative toxicity of Mo, strong oxidative stress (reactive oxygen species increased by 16.5 %), the inhibition of extracellular polymeric substances production and the reduction of microbial activity were proved to be the main inhibition mechanism. In terms of microbial electron transport system, the main electron carriers including flavin mononucleotide (FMN), nitrate reductase (NAR), nitrite reductase (NIR) were seriously inhibited. BugBase analysis confirmed that the relative abundance of biofilm forming bacteria decreased after PMo12 addition, and the relative abundance of oxidative stress tolerance bacteria continued to increase., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)- Published
- 2023
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33. Water-Harvesting Metal-Organic Frameworks with Gigantic Al 24 Units and their Deconstruction into Molecular Clusters.
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Xu H, Wu Y, Yang L, Rao Y, Wang J, Peng S, and Li Q
- Abstract
In contrast to the vast Al-oxo molecular cluster chemistry, Al-based building units for metal-organic framework (MOF) construction are limited in structural diversity and complexity. Synthesis of single crystalline MOFs based on this "hard" metal is further complicated by the poor reversibility of the Al-organic coordination linkages. Here, a strategy to employ two kinds of linkages with distinct strength-strong Al-carboxylate linkage and weak Cu-pyrazol N linkage-gives FDM-91 (FDM=Fudan Materials) with gigantic Al
24 -based units. After replacing the weak moieties with organic linkers post-synthetically, two new stable MOFs with exceptional water harvesting capacity (up to 0.53 g g-1 ) and outstanding cycling performance are developed. Linkage-selective dissociation of FDM-91 further leads to the isolation of the Al24 molecular clusters. The versatile chemistry performed here to reinforce or deconstruct MOFs provides a new way to make important extended and discrete structures., (© 2022 Wiley-VCH GmbH.)- Published
- 2023
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34. Cu/SaBox-Catalyzed Photoinduced Coupling of Acylsilanes with Alkynes.
- Author
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Zheng L, Guo X, Li YC, Wu Y, Xue XS, and Wang P
- Abstract
The transition metal-catalyzed cross-coupling reaction with Fischer metal carbene intermediates bearing an electron-rich alkoxyl or siloxyl group remains a big challenge due to the lack of readily available corresponding carbene precursors. Herein, we report the coupling of alkynes with the Fischer-type copper carbene species bearing a α-siloxyl group, which could be in situ generated from acylsilanes catalytically under photoirradiation and redox-neutral conditions. The side-arm modified bisoxazoline (SaBox) ligands prove to be crucial for this coupling reaction, which provides the corresponding alkynyl alcohol in high yields with remarkable heterocycle tolerance and broad substrate scope., (© 2022 Wiley-VCH GmbH.)
- Published
- 2023
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35. Effect of Noninvasive Static Human Data on Maximum Data in Exercise.
- Author
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Wu Y and Sun Y
- Subjects
- Male, Female, Humans, Heart Rate physiology, Exercise Test methods, Body Composition, Oxygen Consumption physiology, Exercise physiology
- Abstract
Maximum data in exercise (Max-Ex), including maximum heart rate (HRmax), peak oxygen uptake (VO2pk), maximum power (MaxP), etc., are frequently used, whether it is for the determination of exercise intensity, the measurement of an athlete’s performance, assessment of recovery from disease, and so on. However, very often this choice does not take into account the targeted individual. We recruited 32 males and 29 females to undergo an incremental graded exercise test (GXT). Therefore, our study seeks to determine variations in Max-Ex, according to the noninvasive static human data (Non-In data). Data showed a significant relationship (p < 0.001) between body composition and Max-Ex. Of the 41 types of Non-In data we collected in communities, the body composition generally showed high correlation (maximum r = 0.839). 57.5% of the data, of which r > 0.6 were about body composition. The muscle-related body composition data had a greater effect on power, and the fat-related ones had a greater effect on HRmax and VO2pk. For some types of Max-Ex, the older and younger ones showed specific differences. Therefore, these results can be employed to adequately prescribe personalized health promotion programs according to diversity and availability, and have some reference value for other studies using Max-Ex.
- Published
- 2023
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36. Virtual Impactor-Based Label-Free Pollen Detection using Holography and Deep Learning.
- Author
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Luo Y, Zhang Y, Liu T, Yu A, Wu Y, and Ozcan A
- Subjects
- Pollen, Particulate Matter, Aerosols, Holography, Deep Learning
- Abstract
Exposure to bio-aerosols such as pollen can lead to adverse health effects. There is a need for a portable and cost-effective device for long-term monitoring and quantification of various types of pollen. To address this need, we present a mobile and cost-effective label-free sensor that takes holographic images of flowing particulate matter (PM) concentrated by a virtual impactor, which selectively slows down and guides particles larger than 6 μm to fly through an imaging window. The flowing particles are illuminated by a pulsed laser diode, casting their inline holograms on a complementary metal-oxide semiconductor image sensor in a lens-free mobile imaging device. The illumination contains three short pulses with a negligible shift of the flowing particle within one pulse, and triplicate holograms of the same particle are recorded at a single frame before it exits the imaging field-of-view, revealing different perspectives of each particle. The particles within the virtual impactor are localized through a differential detection scheme, and a deep neural network classifies the pollen type in a label-free manner based on the acquired holographic images. We demonstrated the success of this mobile pollen detector with a virtual impactor using different types of pollen (i.e., bermuda, elm, oak, pine, sycamore, and wheat) and achieved a blind classification accuracy of 92.91%. This mobile and cost-effective device weighs ∼700 g and can be used for label-free sensing and quantification of various bio-aerosols over extended periods since it is based on a cartridge-free virtual impactor that does not capture or immobilize PM.
- Published
- 2022
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- View/download PDF
37. Ligand-enabled Ni-catalyzed hydroarylation and hydroalkenylation of internal alkenes with organoborons.
- Author
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Wang DM, She LQ, Wu Y, Zhu C, and Wang P
- Abstract
The transition metal-catalyzed hydrofunctionalization of alkenes offers an efficient solution for the rapid construction of complex functional molecules, and significant progress has been made during last decades. However, the hydrofunctionalization of internal alkenes remains a significant challenge due to low reactivity and the difficulties of controlling the regioselectivity. Here, we report the hydroarylation and hydroalkenylation of internal alkenes lacking a directing group with aryl and alkenyl boronic acids in the presence of a nickel catalyst, featuring a broad substrate scope and wide functional group tolerance under redox-neutral conditions. The key to achieving this reaction is the identification of a bulky 1-adamantyl β-diketone ligand, which is capable of overcoming the low reactivity of internal 1,2-disubstituted alkenes. Preliminary mechanistic studies unveiled that this reaction undergoes an Ar-Ni(II)-H initiated hydroarylation process, which is generated by the oxidative addition of alcoholic solvent with Ni(0) species and sequential transmetalation. In addition, the oxidative addition of the alcoholic solvent proves to be the turnover-limiting step., (© 2022. The Author(s).)
- Published
- 2022
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38. The Validation and Application of a Novel CYP2C19 Genotyping Approach Based on Capillary Electrophoresis in Chinese Han population.
- Author
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Chen D, Wu Y, Wang Y, Yu H, Lai L, and Huang Y
- Subjects
- Humans, Cytochrome P-450 CYP2C19 genetics, Genotype, Reproducibility of Results, Gene Frequency, Alleles, China, Electrophoresis, Capillary, Polymorphism, Single Nucleotide
- Abstract
Background: CYP2C19 gene polymorphisms have been described to have an important influence on the drug metabolism observed in human populations. A series of PCR-based molecule detection methods are applied to identify CYP2C19 genotype. The aim of the study is to validate the novel CYP2C19 genotyping approach with other methods and reveal the allele frequency distribution of CYP2C19 in Chinese Han population., Methods: We applied a novel genotyping approach for CYP2C19 gene which was combining direct PCR and capillary electrophoresis (CE) technique. A series of fluorescent labeled primers were designed to amplify the particular DNA fragments which indicated the wild type of CYP2C19 genotype. The variants consist of CYP2C19*2, CYP2C19*3 and CYP2C19*17 alleles. Both the novel PCR-based CE method and real-time quantitative PCR (RT-qPCR) method were used to identify the CYP2C19 genotypes in 324 whole blood samples originated from Chinese Han population. According to the different criterions for judgement of two methods, we can obtain the CYP2C19 alleles and genotypes of the same participants. Kappa statistics was used to evaluate the consistency of the two results and the frequencies of CYP2C19 alleles. The genotypes in Chinese Han population were calculated using EXCEL. Furthermore, to ensure the accuracy and reliability of the CYP2C19 genotypes obtained by using the novel approach, Sanger sequencing was conducted to validate the CYP2C19 genotypes *1/*17 and *2/*3., Results: Among the 324 specimens, 111 were *1/*1, 141 were *1/*2, 10 were *1/*3, 4 were *1/*17, 46 were *2/*2, 10 were *2*/3, 1 was *2/*17, and 1 was *3/*17. Allele distributions for CYP2C19 were *1, *2, *3, and *17 at 58.18%, 37.65%, 3.24%, and 0.93%, respectively. Both PCR-based CE method and RT-qPCR methods had good consistency in the genotypes of CYP2C19 polymorphism (Kappa value = 1.000, p < 0.05). The DNA sequences of CYP2C19 genotype *1/*17 were composed of c.681 G/G, c.636 G/G, and c.-806 C>T. In the same way, the DNA sequences of CYP2C19 genotype *2/*3 were composed of c.681 G>A, c.636 G>A, and c.-806 C/C., Conclusions: The variants including the CYP2C19*2 allele were the most common mutations in Chinese Han unrelated individuals. Both PCR-based CE method and RT-qPCR method had good consistency in the genotypes of CYP2C19 polymorphism. Nevertheless, because of more convenience and higher throughput, the novel PCR-based capillary electrophoresis approach showed to be more suitable for clinical gene screening.
- Published
- 2022
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39. Nanocomposite Polymeric Membranes for Organic Micropollutant Removal: A Critical Review.
- Author
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Wu Y, Chen M, Lee HJ, A Ganzoury M, Zhang N, and de Lannoy CF
- Abstract
The prevalence of organic micropollutants (OMPs) and their persistence in water supplies have raised serious concerns for drinking water safety and public health. Conventional water treatment technologies, including adsorption and biological treatment, are known to be insufficient in treating OMPs and have demonstrated poor selectivity toward a wide range of OMPs. Pressure-driven membrane filtration has the potential to remove many OMPs detected in water with high selectivity as a membrane's molecular weight cutoff (MWCO), surface charge, and hydrophilicity can be easily tailored to a targeted OMP's size, charge and octanol-water partition coefficient (K
ow ). Over the past 10 years, polymeric (nano)composite microfiltration (MF), ultrafiltration (UF), and nanofiltration (NF) membranes have been extensively synthesized and studied for their ability to remove OMPs. This review discusses the fate and transport of emerging OMPs in water, an assessment of conventional membrane-based technologies (NF, reverse osmosis (RO), forward osmosis (FO), membrane distillation (MD) and UF membrane-based hybrid processes) for their removal, and a comparison to the state-of-the-art nanoenabled membranes with enhanced selectivity toward specific OMPs in water. Nanoenabled membranes for OMP treatment are further discussed with respect to their permeabilities, enhanced properties, limitations, and future improvements., Competing Interests: The authors declare no competing financial interest., (© 2022 The Authors. Published by American Chemical Society.)- Published
- 2022
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40. Silicon-Stereogenic Monohydrosilane: Synthesis and Applications.
- Author
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Wu Y and Wang P
- Abstract
Optically active organosilanes have been demonstrated to be versatile chiral reagents in synthetic chemistry since the early seminal contributions by Sommer and Corriu. Among these silicon-containing chiral architectures, monohydrosilanes, which bear a Si-H bond, hold a unique position because of their facile transformations through stereospecific Si-carbon or Si-heteroatom bond-formation reactions. In addition, those compounds have also been leveraged as chiral reagents for alcohol resolution, chiral auxiliaries, mechanistic probes, as well as potential optoelectronic materials. This Minireview comprehensively summarizes the synthesis and synthetic applications of silicon-stereogenic monohydrosilanes, particularly the advances in the transition-metal-catalyzed asymmetric synthesis of this class of functional molecules., (© 2022 Wiley-VCH GmbH.)
- Published
- 2022
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41. Construction of azaheterocycles via Pd-catalyzed migratory cycloannulation reaction of unactivated alkenes.
- Author
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Wang JP, Song S, Wu Y, and Wang P
- Subjects
- Catalysis, Molecular Structure, Alkenes, Palladium
- Abstract
Azahetereocycles constitute important structural components in many biologically active natural compounds and marketed drugs, and represent the most promising scaffolds in drug discovery. Accordingly, the development of efficient and general synthetic methods for the construction of diverse azaheterocycles is the major goal in synthetic chemistry. Herein, we report the efficient construction of a wide range of azaheterocycles via a Pd-catalyzed migratory cycloannulation strategy with unactivated alkenes. This strategy enables the rapid synthesis of a series of 6-, 7- and 8-membered azaheterocycles in high efficiency, and features a broad substrate scope, excellent functional group tolerance under redox-neutral conditions. The significance of this finding is demonstrated by the efficient synthesis of drug-like molecules with high step-economy. Preliminary mechanistic investigations reveal that this reaction underwent a sequentially migratory insertion to alkenes, metal migration process, and the aza-Michael addition to a quinone methide intermediate., (© 2022. The Author(s).)
- Published
- 2022
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42. Theaflavin-3,3'-Digallate from Black Tea Inhibits Neointima Formation Through Suppression of the PDGFRβ Pathway in Vascular Smooth Muscle Cells.
- Author
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Wu Y, Chen M, Chen Z, Shu J, Zhang L, Hu J, Yu H, Huang K, and Liang M
- Abstract
The abnormal neointima formation caused by the phenotypic switching of vascular smooth cells (VSMCs) into a synthetic state plays a key role in the pathogenesis of various vascular diseases, including atherosclerosis and postangioplasty restenosis. Theaflavin-3,3'-digallate (TF3) in black tea has been reported to exert antiinflammatory and anticancer effects, but its role in neointima formation remains unclear. Here, we delineated a remarkable effect of TF3 in suppressing neointima formation of VSMCs in vivo as well as the ability of primary rat aortic smooth cells (RASMCs) to proliferate and migrate in vitro . Further study confirmed that the effects of TF3 on PDGF-BB-induced RASMCs were due to reduced phosphorylation of PDGFRβ, which led to the repression of downstream pathways. We concluded that TF3 may act as a repressor in the progression of neointima formation and serve as a potential therapeutic candidate for excessive phenotypic switching of VSMCs., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Wu, Chen, Chen, Shu, Zhang, Hu, Yu, Huang and Liang.)
- Published
- 2022
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43. Assessing leached TOC, nutrients and phenols from peatland soils after lab-simulated wildfires: Implications to source water protection.
- Author
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Wu Y, Xu X, McCarter CPR, Zhang N, Ganzoury MA, Waddington JM, and de Lannoy CF
- Subjects
- Canada, Ecosystem, Nutrients, Phenols, Soil, Wildfires
- Abstract
Pollutant leaching from wildfire-impacted peatland soils (peat) is well-known, but often underestimated when considering boreal ecosystem source water protection and when treating source waters to provide clean drinking water. Burning peat impacts its physical properties and chemical composition, yet the consequences of these transformations to source water quality through pollutant leaching has not been studied in detail. We combusted near-surface boreal peat under simulated peat smoldering conditions at two temperatures (250 °C and 300 °C) and quantified the concentrations of the leached carbon, nutrients and phenols from 5 g peat L
-1 reverse osmosis (RO) water suspensions over a 2-day leaching period. For the conditions studied, measured water quality parameters exceeded US surface water guidelines and even exceeded EU and Canadian wastewater/sewer discharge limits including chemical oxygen demand (COD) (125 mg/L), total nitrogen (TN) (15 mg/L), and total phosphorus (TP) (2 mg/L). Phenols were close to or higher than the suggested water supply standard established by US EPA (1 mg/L). Leached carbon, nitrogen and phosphorus mainly came from the organic fraction of peats. Heating peats to 250 °C promoted the leaching of carbon-related pollutants, whereas heating to 300 °C enhanced the leaching of nutrients. Post-heated peats leached higher loads of pollutants in water than pre-heated peats, suggesting that fire-damaged boreal peats may be a critical but underappreciated source of water pollution. A simplified Partial Least Squares (PLS) model based on other easily measured parameters provided a simple method for determining the extent of COD and phenolic pollution in bulk water, relevant for water and wastewater treatment plants. Conclusions from this lab study indicate the need for field measurements of aquatic pollutants downstream of peatland watersheds post-fire as well as increased monitoring and treatment of potable water sources for leachable micropollutants in fire-dominated forested peatlands., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)- Published
- 2022
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44. Daidzein suppresses TGF-β1-induced cardiac fibroblast activation via the TGF-β1/SMAD2/3 signaling pathway.
- Author
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Shu J, Hu L, Wu Y, Chen L, Huang K, Wang Z, and Liang M
- Subjects
- Animals, Cardiovascular Diseases physiopathology, Disease Models, Animal, Dose-Response Relationship, Drug, Fibrosis, Growth Inhibitors administration & dosage, Growth Inhibitors therapeutic use, Humans, Isoflavones administration & dosage, Isoflavones therapeutic use, Male, Mice, Inbred C57BL, Rats, Rats, Sprague-Dawley, Signal Transduction drug effects, Smad2 Protein metabolism, Transforming Growth Factor beta1 metabolism, Mice, Cardiovascular Diseases drug therapy, Fibroblasts drug effects, Growth Inhibitors pharmacology, Isoflavones pharmacology, Myocardium pathology
- Abstract
Myocardial fibrosis is a concomitant bioprocess associated with many cardiovascular diseases (CVDs). Daidzein is an isoflavone that has been used for the treatment of CVDs. This study aimed to reveal its role in myocardial fibrosis. Our results indicate that daidzein had a nontoxic effect on cardiac fibroblasts and that TGF-β1 and TGFβRI levels were gradually decreased by daidzein in a dose-dependent manner. In the current study, we show that daidzein significantly inhibited TGF-β1-induced mRNA and protein expression of α-SMA, collagen I, and collagen III. Accordingly, immunofluorescence staining of α-SMA was performed. Daidzein also inhibited TGF-β1-induced cardiac fibroblast proliferation and migration. Mechanistically, daidzein inhibited the TGF-β/SMAD signaling pathway induced by TGF-β1 in cardiac fibroblasts. Additionally, daidzein ameliorated MI-induced cardiac dysfunction and cardiac fibrosis in vivo. Based on these findings, we conclude that daidzein reduces TGF-β1-induced cardiac fibroblast activation by partially regulating the TGF-β1/SMAD2/3 signaling pathway., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
45. A Capillary Electrophoresis Method for Analyzing the Diversity of Vaginal Microbiome.
- Author
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Lai L, Wu Y, Li Y, and Huang Y
- Subjects
- Adult, Electrophoresis, Capillary, Female, Gardnerella vaginalis genetics, Humans, Middle Aged, RNA, Ribosomal, 16S genetics, Vagina chemistry, Vagina microbiology, Microbiota genetics, Vaginosis, Bacterial diagnosis, Vaginosis, Bacterial microbiology
- Abstract
Background: 16S ribosomal RNA (rRNA) gene sequence analysis is the common method to identify the bacteria in human vaginal flora. While specific DNA primers were designed to target 16S rRNA gene sequences, DNA fragment analysis using capillary electrophoresis can obtain more accurate qualitative and quantitative information on the microbiome. This study aimed to assess the ability of capillary electrophoresis method to analyze the diversity of vaginal microbiome and provide a theoretical basis for the accurate gene detection of vaginal flora., Methods: We collected 75 vaginal secretion samples from female outpatients aged 25 - 50, who had undergone rou-tine gynecologic examinations in Fujian provincial hospital from March 2021 to April 2021. Clinical diagnosis was based on the results of microscopic examination of Gram-stained specimens and biochemical tests of bacteria (pH value, catalase, leukocyte esterase, sialidases, β-glucuronidase, and acetylglucosaminidase). Vaginal secretion samples were collected and then total bacterial DNA was extracted. We used six pairs of fluorescent dye tagged specific primers that were designed based on the 16S rRNA genes of four Lactobacillus species (L. iners, L. crispatus, L. jensenii, L. gasseri), Gardnerella vaginalis, and Atopobium vaginae. PCR products of six species of bacteria were detected and analyzed by a 3130 Genetic Analyzer., Results: Seventy-five samples were divided into two groups according to the vaginal microbiome evaluation, including 50 cases which had vaginal bacteria balance and 25 cases which had vaginal bacteria disorder. PCR amplification of 16S ribosomal RNA genes of L. iners, L. crispatus, L. jensenii, L. gasseri, Gardnerella vaginalis, and Atopobium vaginae was successfully performed on the DNA extracted from vaginal secretion samples. Four Lac-tobacillus species were detected in 4 - 33 cases of "Balance" group, and Gardnerella vaginalis was detected in 23 cases of "Disorder" group and, simultaneously, Atopobium vaginae was detected in 20 cases., Conclusions: Based on the technique of DNA fragment analysis using capillary electrophoresis method, the most common vaginal bacteria in Chinese healthy women are L. iners and L. crispatus. Gardnerella vaginalis and Atopobium vaginae are the most common pathogenic bacteria detected in the patients who had vaginal bacteria disorder. Using capillary electrophoresis method to detect the vaginal bacteria will be useful for accurate identifica-tion of vaginal microbiome. There will be an application value to find out the composition of the vaginal microbiome rapidly and detect specific gene markers to identify potential pathogenic bacteria when women are at risk of serious illness before they develop obvious symptoms.
- Published
- 2022
- Full Text
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46. Enantioselective Synthesis of Silicon-Stereogenic Monohydrosilanes by Rhodium-Catalyzed Intramolecular Hydrosilylation.
- Author
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Huang YH, Wu Y, Zhu Z, Zheng S, Ye Z, Peng Q, and Wang P
- Abstract
Enantiopure monohydrosilanes are versatile chiral reagents for alcohol resolution and mechanistic investigation. Herein, we have demonstrated the asymmetric synthesis of monohydrosilanes via an intramolecular hydrosilylation strategy. This protocol is suitable for the synthesis of five- and six-membered cyclic monohydrosilanes, including a class of chiral oxasilacycles, with excellent diastereo-, regio-, and enantioselectivities. Notably, the catalyst loading could be reduced to 0.1 mol % which makes this one of the most efficient methods to access chiral monohydrosilanes. Mechanistic studies and DFT calculations indicate this Rh-catalyzed intramolecular asymmetric hydrosilylation reaction might proceed via a Chalk-Harrod mechanism, and the enantio-determining step was predicted to be oxidative addition of Si-H bond., (© 2021 Wiley-VCH GmbH.)
- Published
- 2022
- Full Text
- View/download PDF
47. Concise synthesis and applications of enantiopure spirobiphenoxasilin-diol and its related chiral ligands.
- Author
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Yang L, Xu WQ, Liu T, Wu Y, Wang B, and Wang P
- Abstract
The development of chiral architectures for chiral ligand and catalyst discovery is essential for asymmetric catalysis. Herein, we report the concise synthesis of a Si-centered spirocyclic skeleton, spirobiphenoxasilin-diol (SPOSiOL), and its derived chiral ligands. Using the chemical resolution method, the optical SPOSiOL could be obtained in high yield on a gram scale. Preliminary studies indicated that this ligand scaffold has great potential in transition metal-catalyzed asymmetric reactions. This finding further highlights that the Si-centered spirocyclic scaffolds are of great value in asymmetric catalysis.
- Published
- 2021
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48. Construction of α-Amino Azines via Thianthrenation-Enabled Photocatalyzed Hydroarylation of Azine-Substituted Enamides with Arenes.
- Author
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Zhang YL, Wang GH, Wu Y, Zhu CY, and Wang P
- Abstract
α-Amino azines are widely found in pharmaceuticals and ligands. Herein, we report a practical method for accessing this class of compounds via photocatalyzed hydroarylation of azine-substituted enamides with the in situ-generated aryl thianthrenium salts as the radical precursor. This reaction features a broad substrate scope, good functional group tolerance, and mild conditions and is suitable for the late-stage installation of α-amino azines in complex structures.
- Published
- 2021
- Full Text
- View/download PDF
49. The GABA B receptor mediates neuroprotection by coupling to G 13 .
- Author
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Wang Y, Gai S, Zhang W, Huang X, Ma S, Huo Y, Wu Y, Tu H, Pin JP, Rondard P, Xu C, and Liu J
- Subjects
- gamma-Aminobutyric Acid, Neuroprotection, Receptors, GABA-B
- Abstract
G protein–coupled receptors (GPCRs) activate various mitogen-activated protein kinase (MAPK) pathways to regulate critical cell functions. β-Arrestins mediate this mechanism for most GPCRs but not the GABA
B receptor (GABAB R). When coupled to the G protein Gi/o , GABAB R phosphorylates the kinases ERK1 and ERK2. Here, we uncovered a distinct β-arrestin–independent mechanism of MAPK pathway activation by GABAB R. We found that GABAB R also phosphorylated the kinase JNK downstream of activation of the small guanosine triphosphatases (GTPases) RhoA and Rac1 in primary mouse neurons. However, instead of Gi/o proteins, activation of this RhoA/Rac1-JNK pathway was mediated by G13 . This pathway promoted the phosphorylation and accumulation of the postsynaptic scaffolding protein PSD95 and GABAB R-mediated neuroprotection in granule neurons. In addition, this pathway synergized with a previously reported GABAB R-mediated neuroprotection mediated by a Gi/o -dependent mechanism. GABAB R agonists activated G13 with slower kinetics and lower potency than with which they activated Gi/o . Our findings reveal distinct, β-arrestin–independent, context-specific synergistic mechanisms of MAPK activation by G protein–mediated GPCR signaling.- Published
- 2021
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50. Prolonged pituitary downregulation with gonadotropin-releasing hormone agonist improves the live-birth rate: a retrospective cohort study comparing 3 different protocols.
- Author
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Ruan M, Fang L, Yang S, Chen Z, Wu Y, Qu X, Zhao J, and Cheng J
- Subjects
- Down-Regulation, Female, Gonadotropin-Releasing Hormone, Humans, Pregnancy, Retrospective Studies, Birth Rate, Ovulation Induction
- Abstract
Background: The long protocol has been recognized as the gold standard in controlled ovarian hyperstimulation (COH). However, the full dose of gonadotropin-releasing hormone agonist (GnRH-a) under the prolonged protocol has become increasingly popular in China. This study sought to compare pregnancy outcomes among the following 3 groups: a long protocol group, and 2 types of improved prolonged protocol groups., Methods: A retrospective cohort study was conducted of 550 patients undergoing fresh embryo transfer (ET). Patients were treated either with the improved prolonged protocol in the follicular phase (Group 1; n=288) or the mid-luteal phase (Group 2; n=143), or the long protocol (Group 3; n=119). The clinical and laboratory outcomes of the 3 groups were compared., Results: The general characteristics of the women in the 3 groups were comparable. On the day on which gonadotropin (Gn) was first administered and on the day on which human chorionic gonadotropin (hCG) was administered, the luteinizing hormone (LH) levels of patients in both Groups 1 and 2 were lower than those of patients in Group 3. The number of oocytes retrieved, fertilized, and cleaved, and the number of high-quality embryos in the 3 procedures were similar. However, the number of transferred embryos, the rate of blastocyst progression, and the rate of implantation differed. The clinical pregnancy rates (CPRs)were significantly higher in the prolonged protocol groups (62.5% and 61.5%) than the long protocol group (48.7%). Further, statistically significant differences in the live-birth rates (LBRs) (56.9% vs. 57.3% vs. 42.9%) were observed. However, no differences in early abortion rates were found., Conclusions: As a result of pituitary downregulation with GnRH-a, the prolonged groups had better CPRs and LBRs than the long protocol group. The prolonged protocol in the mid-luteal phase was equally effective as that in the early follicular phase in fresh in-vitro fertilization (IVF)/intracytoplasmic sperm injection-embryo transfer (ICSI-ET) cycles. High LH levels on the day of hCG may be a predictor of adverse clinical outcomes.
- Published
- 2021
- Full Text
- View/download PDF
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