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In aquatic ecosystems, light penetrating the sediment surface in shallow lakes may regulate the internal phosphorus (P) release through benthic primary production, which subsequently affects oxidation, pH levels, and alkaline phosphatase activity in the upper sediment. To study the effects of light exposure on the P dynamics at the sediment-water interface under eutrophic conditions, a two-month mesocosm experiment was conducted in twelve cement tanks (1000 L each). The tanks were equipped with Light-Emitting Diode (LED) lights, and surface sediments collected from eutrophic Lake Nanhu (China) were exposed to four different light intensities (0, 50, 100, 200 μmol m -2 s -1 ). The results revealed that: 1) Both the total phosphorus concentration and the phosphorus release flux from the sediment were lower in the light treatments (mean value, 0.59-0.71 mg L -1 and 0.00-0.01 mg m -2 d -1 , respectively) than in the control treatment (0.77 mg L -1 and 0.01 mg m -2 d -1 , respectively), indicating that light supplement could decrease the internal P release. 2) Benthic primary production promoted by light directly absorbed soluble reactive phosphorus and decreased the internal P release. The resulting improved production could also increase dissolved oxygen concentrations at the sediment-water interface, thus indirectly inhibiting internal P release. 3) The relative contributions of direct absorption and indirect inhibition on the internal P release ranged between 23% to 69% and 31% to 77% depending on the light intensity., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

The unprecedented global increase in the anthropogenic-derived nitrogen (N) input may have profound effects on phosphorus (P) dynamics and may potentially lead to enhanced eutrophication as demonstrated in short-term mesocosm experiments. However, the role of N-influenced P release is less well studied in large-scale ecosystems. To gain more insight into ecosystem effects, we conducted a five-year large-scale experiment in ten ponds (700-1000 m 2 each) with two types of sediments and five targeted total N concentrations (TN) by adding NH 4 Cl fertilizer (0.5, 1, 5, 10, and 25 mg N L -1 ). The results showed that: (ⅰ) The sediment P release increased significantly when TN exceeded 10-25 mg N L -1 . (ⅱ) The most pronounced sediment P release increase occurred in summer and from sediments rich in organic matter (OM Sed ). (ⅲ) TN, algal biomass, fish biomass, non-algal turbidity, sediment pH, and OM Sed were the dominant factors explaining the sediment P release, as suggested by piecewise structural equation modeling. We propose several mechanisms that may have stimulated P release, i.e. high ammonium input causes a stoichiometric N:P imbalance and induce alkaline phosphatase production and dissolved P uptake by phytoplankton, leading to enhanced inorganic P diffusion gradient between sediment and water; higher pelagic fish production induced by the higher phytoplankton production may have led increased sediment P resuspension through disturbance; low oxygen level in the upper sediment caused by nitrification and organic decomposition of the settled phytoplankton and, finally, long-term N application-induced sediment acidification as a net effect of ammonium hydrolysis, nitrification, denitrification; The mechanisms revealed by this study shed new light on the complex processes underlying the N-stimulated sediment P release, with implications also for the strategies used for restoring eutrophicated lakes., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

The toxic effect of unionized ammonia (NH 3 ) on aquatic organisms is receiving increasing attention due to the excessive nitrogen discharge to various surface waters. Researches have suggested the scale-dependence of NH 3 toxicity, being lower in field than under lab conditions. Such scale-dependence of toxicity is a big challenge to water quality criteria setting as the results solely from lab tests might not apply to natural ecosystems. Therefore, it is necessary to explore the underlying mechanism to understand the difference of toxicity across various spatial scales. In this study, we used the widely distributed gastropod Bellamya aeruginosa as the test animal and performed two 192-h microcosm experiments. Each experiment included a control and an ammonia addition treatment: N0(LC 50 ) & N+(LC 50 ), N0(LC 100 ) & N+(LC 100 ) (96-h LC 50  = 0.8 mg NH 3 N/L, 96-h LC 100  = 18.1 mg NH 3 N/L). Besides water-only, three potential key components (food, sediment, and submersed macrophytes) were included in the various treatments to mimic different complexity levels of aquatic ecosystems (Water, Water + Food, Water + Sediment, Water + Sediment + Macrophytes). The results showed that: 1) food directly improved the survival and growth of gastropods under expected lethal concentration of ammonia (96-h concentration of NH 3 N = LC 20 of the 96-h acute test); 2) sediment and macrophyte quickly decreased the ammonia concentration, mainly by sediment adsorption and macrophyte uptake, to alleviate the ammonia stress to gastropods and permitted them to survive and grow under expected lethal concentration of ammonia (96-h concentration of NH 3 N = LC 10 ∼LC 20 of the 96-h acute test); 3) sediment and macrophyte also provided additional food for gastropods; 4) under extremely high ammonia stress (i.e., 96-h LC 100 , food was left uneaten and macrophyte died, and gastropods could, therefore, not be released from ammonia stress. Our results demonstrate that under moderate ammonia stress, the introduction of extra ecosystem elements (food, sediment, and macrophytes) significantly improved the survival and growth of gastropods, mainly by enhancing their tolerance and by quickly decreasing the NH 3 concentration and thus toxicity. However, these introduced elements had little effect at very high concentration of ammonia (i.e., 96-h LC 100 ). Our findings add to the understanding of the reasons behind the previous observed scale-dependent toxicity of NH 3 on aquatic organisms and contribute to better decisions on the role of NH 3 in relation to water quality management., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Along with a steady increasing use of artificial nitrogen fertilizer, concerns have been raised about the effects that high nitrogen loading may have on ecosystems. Due to the toxicity of unionized ammonia (NH 3 ), tolerance criteria have been proposed for ambient water management in many countries; however, these are mainly based on acute or chronic tests carried out under lab conditions run with purified water. Aiming at understanding the responses of organisms to natural exposure to high ammonia concentrations, a Viviparidae gastropod, Bellamya aeruginosa, was tested at three experimental scales: standard 96-h lab test, one-month cage test in 6 experimental ponds with continuous nitrogen inputs, and intensive investigation of the B. aeruginosa from these ponds in spring and winter. The results were: 1) 96-h LC 50 in the standard lab test was 0.56 mg NH 3 -N/L and 343.3 mg TAN/L (total ammonia expressed as N, standardized at pH 7 and 20 ℃). 2) In the one-month cage test, the survival rate was 97% when NH 3 -N was 0.61 mg/L (i.e., a higher concentration than the lab 96-h LC 50 ) and the body size of the gastropods actually increased with increasing NH 3 -N concentrations. 3) In the winter-spring investigation, little effect of ammonia on the standing crops of gastropods was found, and the body size of the gastropods tended to increase with increasing ammonia concentrations (NH 3 -N concentration range of 0.05 ~ 2.06 mg/L). Thus, B. aeruginosa showed higher tolerance to ammonia exposure (NH 3 -N concentration < 0.81 mg/L) in the field than under laboratory conditions. Our study points to the necessity of considering the relevant scale when determining criteria for ammonia toxicity in water management., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)

Urban lakes are important natural assets but are exposed to multiple stressors from human activities. Submersed macrophytes, a key plant group that helps to maintain clear-water conditions in lakes, tend to be scarce in urban lakes, particularly when they are eutrophic or hypertrophic, and their loss is linked, in part, to impaired underwater light climate. We tested if enhancing the underwater light conditions using light-emitting diodes (LEDs) could restore submersed macrophytes in urban lakes. Twelve mesocosms (1000 L each) were each planted with tape grass (Vallisneria natans) and monitored over three months (22 August-7 November), using a control and three artificial light intensity treatments (10, 50, and 100 µmol m -2 s -1 ). Compared with the control, the high light treatment (100 µmol m -2 s -1 ) had higher leaf number, maximum leaf length, and average leaf length (3.9, 5.8, and 2.8 times, respectively). Shoot number, leaf number, leaf dry mass, root dry mass, and photosynthetic photon flux density in the high-light treatment were significantly greater than the control, but root length and phytoplankton chlorophyll a were not related to plant growth variables and were low in all treatments. Periphyton chlorophyll a increased significantly with the plant growth variables (i.e., shoot number, leaf number, and maximum leaf length) and was high in the light treatments but did not hamper the growth of the macrophytes. These results indicate that LED light supplementation enables the growth of V. natans under eutrophic conditions, at least in the absence of fish as in our experiment, and that the method may have potential as a restoration method in urban lakes. Lake-scale studies are needed, however, to fully evaluate LED light supplementation under natural conditions where other stressors (e.g., fish grazing) may need to be controlled for successful restoration of urban lakes., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)

Phosphorus (P) release from sediment is a key process affecting the effectiveness of eutrophication mitigation. We hypothesized that high nitrate (NO 3 - ) input may have dual effect on sediment P release: reduce the sediment P release by improving the oxidation of sediment or promote P release by stimulating the growth of phytoplankton and increase the decomposition rates and oxygen consumption at the sediment water interface. To test the effect of different NO 3 - concentrations, we conducted a three-month experiment in 15 cement tanks (1 m 3 ), with five targeted concentrations of NO 3 - : control, 2 mg L -1 , 5 mg L -1 , 10 mg L -1 , and 15 mg L -1 . The results showed that: i) when NO 3 - was maintained at high levels: NO 3 - ≥5-7 mg L -1 (range of median values), there was no effect of NO 3 - on net P release from the sediment, likely because the positive effects of NO 3 - (increasing oxidation) was counteracted by a promotion of phytoplankton growth. ii) after NO 3 - addition was terminated NO 3 - dropped sharply to a low level (NO 3 - ≤0.4 mg L -1 ), followed by a minor P release in the low N treatments but a significant P release in the high N treatments, which likely reflect that the inhibition effect of NO 3 - on P release decreased, while the promotion effects at high NO 3 - concentrations continued. The results thus supported our hypotheses of a dual effect on sediment P release and suggest dose-dependent effect of NO 3 - loading on stimulating P release from the sediment, being clear at high NO 3 - exceeding 5-7 mg L -1 ., Competing Interests: Declaration of Competing Interest No conflict of interests needs to be declared for the manuscript entitled., (Copyright © 2021. Published by Elsevier Ltd.)

Due to excess nutrient loading, loss of submersed macrophytes is a worldwide phenomenon in shallow lakes. Phosphorus is known to contribute significantly to macrophyte recession, but the role of nitrogen has received increasing attention. Our understanding of how high nitrogen concentrations affect the growth of submersed macrophytes, particularly under natural conditions, is still limited. In this study, we conducted experiments with canopy-forming Potamogeton crispus in 10 ponds subjected to substantial differences in nitrogen loading (five targeted total nitrogen concentrations: control, 2, 10, 20, and 100 mg L -1 ) and compared the results with those of our earlier published experiments with rosette-forming Vallisneria natans performed 1 year before. Canopy-forming P. crispus was more tolerant than rosette-forming V. natans to exposure to high NH 4 concentrations. This is probably because canopy-forming species reach the water surface where there is sufficient light for production of carbohydrates, thereby allowing the plants to partly overcome high NH 4 stress. Both the canopy-forming P. crispus and the rosette-forming V. natans showed clear declining trends with increasing chlorophyll a in the water. Accordingly, shading by phytoplankton might be of key importance for the decline in submersed macrophytes in this experiment. Both experiments revealed free amino acids (FAA) to be a useful indicator of physiological stress by high ammonium but is not a reliable indicator of macrophyte growth.

In aquatic ecosystems, ammonium is one of the dominant substances in the effluent discharge from wastewater treatment plants and its impact has been widely explored as it is thought, in its toxic form (NH 3 ), to cause stress on organisms. Little is, however, known about its potential effect on the release of phosphorus (P) from the sediment. In a two-month mesocosm (150 L) experiment, we tested if high loading of ammonium promotes sediment P release and investigated the dominant underlying mechanisms. A gradient of five target ammonium loading levels was used by adding NH 4 Cl fertilizer: no addition/control (N0), 3 (N1), 5 (N2), 10 (N3), and 21 (N4) mg NH 4 Cl L -1 (NH 4 Cl expressed as nitrogen). We found that: 1) significant sediment P release for N3 and N4 but minor release or retention for N0, N1, and N2 were detected both by the total phosphorus concentration (TP) in the overlying water and in situ measurements of diffusive gradients in thin-films (DGT) at the sediment-water interface; 2) overall, TP correlated significantly and positively with total nitrogen (TN) concentrations in the water. Further correlation and path analyses suggested that stimulated alkaline phosphatase activity (APA) was likely the dominant mechanisms behind the ammonium-induced sediment P release and decreased dissolved oxygen (DO) levels (an approximate reduction from 9.2 to 6.6 mg O2 L -1 ) was likely a contributing factor, particularly in the beginning of the experiment., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Loss of submersed macrophytes is a world-wide phenomenon occurring when shallow lakes become eutrophic due to excess nutrient loading. In addition to the well-known effect of phosphorus, nitrogen as a trigger of macrophyte decline has received increasing attention. The precise impact of high nitrogen concentrations is debated, and the role of different candidate factors may well change over the season. In this study, we conducted experiments with Vallisneria natans during the growing season (June-September) in 10 ponds subjected to substantial differences in nitrogen loading (five targeted total nitrogen concentrations: control, 2, 10, 20, and 100mgL -1 ) and compared the results with those obtained in our earlier published study from the low-growth season (December-April). Like in the low-growth season, growth of V. natans in summer declined with increasing ammonium (NH 4 ) concentrations and particularly with increasing phytoplankton chlorophyll a (Chla Phyt ). Accordingly, we propose that shading by phytoplankton might be of key importance for macrophyte decline, affecting also periphyton growth as periphyton chlorophyll a (Chla Peri ) decreased with increasing Chla Phyt . Free amino acid contents (FAA) of plants tended to increase with increasing NH 4 concentrations, while the relationships between FAA with growth indices were all weak, suggesting that FAA might be a useful indicator of the physiological stress of the plants but not of macrophyte growth. Taken together, the results from the two seasons indicate that although a combination of high nitrogen concentrations (ammonium) and shading by phytoplankton may cause severe stress on macrophytes, active growth in the growing season enabled them to partly overcome the stress., (Copyright © 2017 Elsevier B.V. All rights reserved.)

A number of studies have revealed ammonia to be toxic to aquatic organisms; however, little is known about its effects under natural conditions. To elucidate the role of ammonia, we conducted 96-h acute toxicity tests as well as a whole-ecosystem chronic toxicity test for one year in ten 600-m 2 ponds. Three common cyprinids, silver carp Hypophthalmichthys molitrix Val. (H.m.), bighead carp Aristichthys nobilis Richardson (A.n.), and gibel carp Carassius auratus gibelio Bloch (C.g.), were used as test organisms. The 96-h LC 50 values of un-ionized ammonia (NH 3 ) for H.m., A.n., and C.g. were 0.35, 0.33, and 0.73mgL -1 , respectively. In the ponds, annual mean NH 3 ranged between 0.01 and 0.54mgL -1 , with 4 ponds having a NH 3 higher than the LC 50 of A.n. (lowest LC 50 in this study). No fish were found dead in the high-nitrogen ponds, but marked histological changes were found in livers and gills. Despite these changes, the specific growth rate of H.m. and A.n. increased significantly with NH 3 . Our pond results suggest that fish might be more tolerant to high ammonia concentrations in natural aquatic ecosystems than under laboratory conditions. Our finding from field experiments thus suggests that the existing regulatory limits for reactive nitrogen (NH 3 ) established from lab toxicity tests might be somewhat too high at the ecosystem conditions. Field-scale chronic toxicity tests covering full life histories of fish and other aquatic organisms are therefore encouraged in order to optimize determination of the effects of ammonia in natural environments., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Objective: To investigate the expression of 17 beta-hydroxysteroid dehydrogenase type 1 (17β-HSD1) in the kidney of rats and explore the capacity of the kidney for synthesizing sex hormones., Methods: The expressions of 17-HSD1 and sex hormones were detected by Western blotting and radioimmunoassay in rat renal cells in primary cultured for 24 and 48 h in the presence or absence of follicle-stimulating hormone (FSH) and luteinizing hormone (LH)., Results: After cell culture for 24 h, the primary rat renal cells expressed a low level of 17β-HSD1 (0.1843±0.076), which increased to 1.6651±0.044 (P<0.01) in response to co-stimulation by FSH and LH. Low levels of estradiol, progesterone and testosterone were also detected in rat renal cells (3.30±3.78, 62.60±12.33, and 22.12±3.36, respectively), and co-stimulation of FSH and LH significantly increased their levels to 8.50±2.64, 117.80±9.79, and 45.04±4.39, respectively (P<0.05). The levels of these hormones showed no significant differences between cells cultured for 24 h and 48 h (P>0.05)., Conclusion: The rat renal cells express 17β-HSD1 and are capable of stably secreting sex hormones in response to co-stimulation with FSH and LH, suggesting the capacity of the rat kidneys for synthesizing sex hormones. These findings enrich the understanding of the endocrine function of the kidney.

Eutrophication of lakes leading to loss of submersed macrophytes and higher turbidity is a worldwide phenomenon, attributed to excessive loading of phosphorus (P). However, recently, the role of nitrogen (N) for macrophyte recession has received increasing attention. Due to the close relationship between N and P loading, disentanglement of the specific effects of these two nutrients is often difficult, and some controversy still exists as to the effects of N. We studied the effects of N on submersed macrophytes represented by Vallisneria natans (Lour.) Hara in pots positioned at three depths (0.4 m, 0.8 m, and 1.2 m to form a gradient of underwater light conditions) in 10 large ponds having moderate concentrations of P (TP 0.03 ± 0.04 mg L(-1)) and five targeted concentrations of total nitrogen (TN) (0.5, 2, 10, 20, and 100 mg L(-1)), there were two ponds for each treatment. To study the potential shading effects of other primary producers, we also measured the biomass of phytoplankton (ChlaPhyt) and periphyton (ChlaPeri) expressed as chlorophyll a. We found that leaf length, leaf mass, and root length of macrophytes declined with increasing concentrations of TN and ammonium, while shoot number and root mass did not. All the measured growth indices of macrophytes declined significantly with ChlaPhyt, while none were significantly related to ChlaPeri. Neither ChlaPhyt nor ChlaPeri were, however, significantly negatively related to the various N concentrations. Our results indicate that shading by phytoplankton unrelated to the variation in N loading and perhaps toxic stress exerted by high nitrogen were responsible for the decline in macrophyte growth., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

Mollusk biomass is usually expressed as wet mass with shell, but this expression fails to represent real biomass due to the high calcium carbonate content in shells. Tissue dry mass and ash free dry mass are relatively close to real biomass. However, the determination process of these two parameters is very complicated, and thus, it is necessary to establish simple and practical conversion methods for these two parameters. A total of six taxa of freshwater snails (Bellamya sp., Alocinma longicornis, Parafossarulus striatulus, Parafossarulus eximius, Semisulcospira cancellata, and Radix sp.) common in the Yangtze Basin were selected to explore the relations of their five shell dimension parameters, dry and wet mass with shells with their tissue dry mass and ash free dry mass. The regressions of the tissue dry mass and ash free dry mass with the five shell dimension parameters were all exponential (y = ax(b)). Among them, shell width and shell length were more precise (the average percentage error between observed and predicted value being 22.0% and 22.5%, respectively) than the other three parameters in the conversion of dry mass. Wet mass with shell could be directly converted to tissue dry mass and ash free dry mass, with an average percentage error of 21.7%. According to the essence of definition and the errors of conversion, ash free dry mass would be the optimum parameter to express snail biomass.

Potamothrix scleropenis sp. nov. (Tubificidae: Tubificinae) is described from the profundal zone (74 m) of Fuxian Lake, the deepest lake (up to 155 m) on the Yunnan-Guizhou Plateau in China. The new species is assigned to Potamothrix because of its short vasa deferentia and its tubular atria without ejaculatory ducts and prostate glands. It differs from congeners by its cuticularized penis sheaths; bifurcated, strongly curved spermathecal chaetae; bifurcated lower prongs of bifids; and feathered hairs. P. scleropenis appears closely related to P. cekanovskajae Finogenova, 1972 and P. tudoranceai Sporka, 1994, since all the three species have homogeneous atria without prostate glands.