1. L-Asparaginase from Streptomyces griseus NIOT-VKMA29: optimization of process variables using factorial designs and molecular characterization of L-asparaginase gene.
- Author
-
Meena B, Anburajan L, Sathish T, Vijaya Raghavan R, Dharani G, Vinithkumar NV, and Kirubagaran R
- Subjects
- Escherichia coli genetics, Escherichia coli metabolism, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Streptomyces griseus genetics, Asparaginase biosynthesis, Asparaginase chemistry, Asparaginase genetics, Asparaginase isolation & purification, Bacterial Proteins biosynthesis, Bacterial Proteins chemistry, Bacterial Proteins genetics, Bacterial Proteins isolation & purification, Streptomyces griseus enzymology
- Abstract
Marine actinobacteria are known to be a rich source for novel metabolites with diverse biological activities. In this study, a potential extracellular L-asparaginase was characterised from the Streptomyces griseus NIOT-VKMA29. Box-Behnken based optimization was used to determine the culture medium components to enhance the L-asparaginase production. pH, starch, yeast extract and L-asparagine has a direct correlation for enzyme production with a maximum yield of 56.78 IU mL(-1). A verification experiment was performed to validate the experiment and more than 99% validity was established. L-Asparaginase biosynthesis gene (ansA) from Streptomyces griseus NIOT-VKMA29 was heterologously expressed in Escherichia coli M15 and the enzyme production was increased threefold (123 IU mL(-1)) over the native strain. The ansA gene sequences reported in this study encloses several base substitutions with that of reported sequences in GenBank, resulting in altered amino acid sequences of the translated protein.
- Published
- 2015
- Full Text
- View/download PDF