Search

Competing Interests: Competing interests: None declared.

Development, rapid detection and quantification of anticancer drugs in biological samples are crucial for effective drug monitoring. The present work describes the design of a Hf(IV)-based metal-organic framework (MOF) (1) by the reaction between Hf(IV) ion and 2-(thiophene-2-carboxamido)terephthalic acid linker with the surface area of 571 m 2  g -1 . Desolvated MOF (1') displayed highly discriminative fluorescence sensing properties for the antineoplastic drug flutamide and biomolecule hemin in an aqueous medium in the presence of co-existing biomolecules and ions. The MOF's response time for sensing flutamide and hemin was less than 5 s with low detection limits of 1.5 and 0.08 nM, respectively. Additionally, 1' also demonstrated recyclability up to five cycles and maintained its sensing ability across different pH media, various water samples, and biological fluids. Experimental and theoretical analyses suggested photoinduced electron transfer and inner-filter effect in the presence of flutamide and Förster resonance energy transfer in the presence of hemin are most likely reasons behind the fluorescence quenching of MOF. Furthermore, the MOF demonstrated catalytic activity in Friedel-Crafts alkylation reactions, providing a 96 % yield with slight decay in its activity over four uses. The enhanced activity of 1' compared to Hf-BDC and Hf-BDC-NH 2 (BDC: 1,4-benzenedicarboxylic acid) is due to the functionalized thiophene moieties through hydrogen bond donating sites, confirmed by a series of control experiments., (© 2024 Wiley-VCH GmbH.)

Subacute sclerosing panencephalitis (SSPE) is a fatal disorder that occurs as a rare complication of childhood measles. Symptoms typically manifest between the ages of 5 and 15. While the incidence of SSPE is declining globally, it is still prevalent in regions where measles remains common and vaccination rates are low due to poverty and lack of health education. Diagnosing SSPE can be challenging, particularly when patients exhibit unusual symptoms. A thorough clinical evaluation, including vaccination history, physical examination, electroencephalogram (EEG) and Cerebrospinal fluid (CSF) analysis, can help in making a diagnosis. We present the case of a young woman in her early 20s who initially experienced depressive symptoms, followed by myoclonus, dementia and visual impairment. The patient was ultimately diagnosed with SSPE based on characteristic EEG findings, neuroimaging results, CSF analysis and elevated serum measles antibody levels., Competing Interests: Competing interests: None declared., (© BMJ Publishing Group Limited 2024. No commercial re-use. See rights and permissions. Published by BMJ.)

Dental implant restorations attached to cement can potentially result in peri-implant mucositis and peri-implantitis if cement residues are present. Effectively predicting and eliminating such dental cement residues is crucial for preventing complications. This study focuses on creating a regression model using the pixel values to predict the Excess Cement Residues (ECR) by employing an octagonal surface imaging approach. A model featuring gingival imitation, ten abutments, and ten crowns was created, and the cemented implants underwent thorough photographic and analytical assessment. The ECR was determined through two distinct approaches: the Computerized Planimetric Method (CPM) and the weighing method. Across ten implants in this in vitro study, ECR varied from 0.3 to 21 mg, with an average of 5.69 mg. The findings reveal a higher amount of ECR on the distal, mesiobuccal, and mesial sides. Utilizing Pearson's correlation, a coefficient value of r  = 0.786 signifies a strong correlation between CPM and the weighing method. The regression model further aids in predicting ECR based on pixel values. The octagonal surface imaging approach not only vividly captures information about ECR in the implant cementation region but also emphasizes the feasibility of ImageJ as an effective tool for detecting ECR. The congruence between CPM and the weighing method results supports the application of the regression model for precise ECR prediction., Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Background: There are no reliable methods in clinical practice to diagnose adrenal insufficiency (AI) in patients with cirrhosis owing to variable cortisol-binding protein levels. This leads to unreliable results in ACTH stimulated serum cortisol test. We aimed to estimate the long-acting porcine (LA)ACTH-stimulated serum and salivary cortisol levels of patients at different stages of cirrhosis using second generation electrochemiluminescence and to determine the prevalence of true adrenal insufficiency in these patients., Design, Patients and Measurements: We included 135 noncritical patients with cirrhosis (45 each from CHILD A, B and C) and 45 healthy controls. Serum and salivary samples were collected at baseline in the morning and at 1 and 2 h after LA-ACTH injection., Results: In healthy subjects, the 2.5th centile of 2 h ACTH stimulated serum and salivary cortisol were 19.8 and 0.97 μg/dL, which were used as cut-offs for defining AI based on serum and saliva respectively. The median (interquartile-range) 2-h stimulated salivary cortisol in Child A, B, C categories and controls were 1.36(1.23-2.38), 1.46(1.18-2.22), 1.72(1.2-2.2) and 2.12(1.42-2.72) μg/dL respectively. Six subjects (4.4%) were diagnosed to have AI based on stimulated salivary cortisol cut-off, whereas 39 (28.9%) cirrhosis subjects had inadequately stimulated serum cortisol. Three patients (symptomatic) required steroid replacement therapy. Hypoalbuminemia was identified as a major risk factor for the misdiagnosis of adrenal insufficiency by serum cortisol-based testing., Conclusions: Long-acting porcine ACTH stimulated salivary cortisol reduces the overdiagnosis of adrenal insufficiency compared to serum cortisol in cirrhosis liver. Stimulated salivary cortisol is a promising investigation for evaluation of adrenal function in cirrhosis and more studies are required for its further validation before clinical use., (© 2024 John Wiley & Sons Ltd.)

Dental cement residues exacerbate peri-implant tissue irritation and peri-implantitis. The present study aims to evaluate the cytotoxicity, physiochemical, optical, and rheological properties of carbon quantum dots (CQDs) impregnated glass ionomer cement (GIC). Surface passivated fluorescent CQDs were synthesized using citric acid via thermal decomposition and blended with GIC. Characterization studies and rheological measurements were made to evaluate their performance. 3D-printed dental implant models cemented with GIC and GIC-CQD were compared to analyze excess cement residues. MTT assay was performed with human dental pulp stem cells (hDPSCs) and statistically analyzed using ANOVA and Tukey's test. CQDs with a particle dimension of ∼2 nm were synthesized. The amorphous property of GIC-CQD was confirmed through XRD. The fluorescence properties of GIC-CQD showed three times higher emission intensity than conventional GIC. GIC-CQD attained maturation with a setting time extended by 64 s than GIC. Cement residue of size 2 mm was detected with a UV light excitation at a distance between 5 to 10 cm. Biocompatibility at 0.125 mg ml -1 dilution concentrations of GIC-CQD showed viability greater than 80% to hDPSCs. For the first time, we report that CQDs-impregnated GIC is a unique and cost-effective strategy for in-situ detection of excess cement rapidly using a hand-held device. A novel in-situ rapid detection method enables the dentist to identify residual cement of size less than 2 mm during the implantation. Therefore, GIC-CQD would replace conventional GIC and help in the prevention of peri-implant diseases., (© 2024 IOP Publishing Ltd.)

This study investigates the enhancement of hydrogen gas-sensing performance by introducing silver (Ag) nanoparticles onto tungsten trioxide (WO 3 ) thin films. Herein, the WO 3 thin films are deposited onto SiO 2 /Si substrates using a sputtering technique and Ag nanoparticles are loaded onto the WO 3 surface through a spin coating technique. To evaluate the sensing performance of a hydrogen gas, interdigitated titanium (Ti) electrodes are deposited onto the Ag:WO 3 layer. Structural, chemical, and morphological analyses are conducted for both pristine WO 3 and Ag:WO 3 thin films, followed by the investigation of gas-sensing performance by varying hydrogen gas concentrations from 100 ppm to 300 ppm and operating temperatures between 30 °C and 300 °C. The obtained results demonstrate that Ag:WO 3 thin films exhibit a notably enhanced response of 5.08% when exposed to a concentration of 100 ppm of hydrogen gas at room temperature, compared to the pristine WO 3 of 3.40%. The fabricated Ag:WO 3 sensor exhibits a response time of 3.0 s, a recovery time of 4.5 s, and also demonstrates excellent stability over 45 days period. Finally, with the superior sensitivity and fast response time, the fabricated Ti/Ag:WO 3 /Ti hydrogen gas sensor test-device can be a potential for improvement of safety from both industrial and environmental perspectives., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

This study proposes a mecanum-built perturbation-based balance training device aimed at improving motor adaptive skills for fall prevention in individuals with neurological disorders or the elderly. Incorporating multidirectional fall simulations in line with modified constraint-induced movement therapy, the device's efficacy was evaluated by measuring the distance traveled and peak acceleration under different static loads (20, 30, and 40 kg) and input accelerations (1, 2, and 3 m/s 2 ). A pilot study with 10 subjects was conducted to assess device performance, utilizing repeated measures analysis of variance and Bonferroni's post hoc analysis. Results indicated a load-dependent reduction in distance traveled, with an average mean difference of 0.74-1.23 cm between the 20 and 40 kg loads for trials of 9 and 18 cm, respectively. Despite varying loads, the device consistently achieved near-anticipated peak accelerations, suggesting its capability to induce effective perturbations. The study also observed a significant lateral movement preference, suggesting adjustments to pulse width modulation and time period may optimize lateral movement performance., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2024 Jaison Jacob Mathunny et al.)

Competing Interests: Competing interests: None declared.

Background Glass ionomer cement (GIC) demonstrates biocompatibility and fluoride ion release, indicating their potential to inhibit a wide range of bacteria, although this remains uncertain. Lemongrass and neem are recognized for their potent antimicrobial activity against numerous pathogenic microorganisms. The objective of the study is to evaluate the antimicrobial effectiveness and compressive strength of GIC modified with neem and lemongrass. Methodology Lemongrass and neem were incorporated into conventional GIC at varying concentrations. Group I - neem-modified GIC (0.5%, 1%, 2%), group II - lemongrass-modified GIC (0.5%, 1%, 2%), and group III (non-modified GIC as a control group). The disk-shaped specimens were then compared to unmodified GIC (control). Antimicrobial effectiveness was assessed using the minimal inhibitory concentration (MIC) assay against Streptococcus mutans and Lactobacillus . Compressive strength was assessed using a Universal Testing Machine, with a crosshead speed set to 0.5 mm per minute. Statistical analysis was conducted with a significance level set at p < 0.05. Results Neem modification displayed superior antimicrobial effectiveness against both Streptococcus mutans and Lactobacillus at all concentrations when compared to the control, with 2% showing the least mean value of 0.262. In contrast, lemongrass modification exhibited a significant difference in effectiveness against Streptococcus mutans but no difference against Lactobacillus . Neem modification demonstrated superior performance compared to lemongrass (p < 0.05). Both modified groups showed no significant impact on compressive strength. Conclusions Neem-modified GIC demonstrated the highest antimicrobial efficacy against Streptococcus mutans and Lactobacillus without altering its compressive strength. This suggests its potential as a promising alternative material in restorative dentistry. Additional in vivo investigations are needed to assess the extended-term effectiveness of the material., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2024, Jaikumar Ram et al.)

A stroke is a neurological disease that primarily causes paralysis. Besides paraplegia, all other types of paralysis affect the upper extremity. Advanced technologies, such as wearable devices and rehabilitation regimens, are also being developed to enhance the functional ability of a stroke person to grasp and release daily living objects. In this research, we developed a rehabilitation functional assist device combining a flexion and extension mechanism with suction cup technology (hybrid technology) to help post-stroke patients improve their hand grip strength in day-to-day grasping activities. Ten poststroke hemiplegia patients were studied to test the functional ability of the impaired hand by wearing and not wearing the device. The outcomes were validated by three standard clinical tests, such as the Toronto Rehabilitation Institute - Hand Functional Test (TRI-HFT), the Chedoke Arm Hand Activity Inventory (CAHAI-9), and the Fugl-Meyer Assessment (FMA) with overall score improvements of 14.5 ± 3.8-25 ± 2.2 ( p  = 0.005), 5.4 ± 2.8-10 ± 1.6 ( p  = 0.008), and 9.6 ± 2.6-17 ± 2.4 ( p  = 0.005) respectively. The p -value for each of the three evaluations was less than 0.05, indicating significantly improved results and the average feedback score of the participants was 3.8 out of 5. The proposed device significantly increased impaired hand functionality in post-stroke patients. The subjects could complete some of the grasping tasks that they could not grasp without the device. Clinical trial registration The Clinical Trial Registry of India approved the work CTRI/2022/02/040495 described in this manuscript., Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Modern cancer therapy now routinely employs the blocking of many oncoproteins or pathways. With two or more medicines, multiple inhibitions are often accomplished via DML techniques. In this study, we developed 30 quinazolinone derivatives as PARP1 and STAT3 dual inhibitors using DML methods and these compounds were tested for their dual inhibitory effect on PARP1 and STAT3 using docking, MM-GBSA, and molecular dynamics simulation investigations. The docking studies of ligands against PARP1 and STAT3 were performed using the Glide module, in silico ADMET screening was performed using the quickprob module, binding energy calculation was performed using the Prime MM-GBSA module, MD simulation was performed using the Desmond module, and atomic charges were calculated using the Jaguar module of Schrodinger suite 2019-4. Of the substances studied, the derivative 1f has a considerable gliding score. The in silico ADMET characteristics are within the approved range. PRIME MM-GB/SA was used to compute the binding free energy, and the results are substantial. To investigate the dynamic behavior of the protein-ligand complex, compound 1f was subjected to MD simulation at 100 ns. The tested chemical 1f produced the greatest results in MD simulations and MM-GBSA calculations, indicating that this ligand can inhibit more effectively.Communicated by Ramaswamy H. Sarma.

Diabetic striatopathy is a clinicoradiological syndrome characterised by acute hyperkinetic movement disorder in the form of hemichorea-hemiballism with basal ganglia abnormalities in neuroimaging. The hallmark basal ganglia abnormalities appear as hyperdensities in CT brain and hyperintensities in MRI brain, which could mislead the clinician towards an erroneous diagnosis of cerebral haemorrhage. It is classically described in elderly patients with type 2 diabetes mellitus, and its occurrence in type 1 diabetes is extremely rare. This case report entails the clinical details of a young man in his 20s with type 1 diabetes mellitus who had uncontrolled blood glucose levels and presented with a recent onset of abnormal movements in his left upper and lower limbs. The semiology, biochemistry and radiological investigation findings and treatment are detailed. A clear understanding of the condition could lead to an early diagnosis, spare the patient unnecessary investigations and improve treatment outcomes., Competing Interests: Competing interests: None declared., (© BMJ Publishing Group Limited 2023. No commercial re-use. See rights and permissions. Published by BMJ.)

India is one among the major leather-producing countries in the world which shares close to one-fourth of the world's leather solid wastes and most of these wastes are not effectively utilized. These wastes are rich in protein and lipids that could be a potential feedstock for biofuel production, i.e., biogas, biodiesel, etc. Among the 150,000 tons of daily leather solid wastes in India, approximately 87,150 tons are shared by pre-tanning operations (i.e., raw trimmings, fleshing, and hair wastes) while the rest of the 62,850 tons are shared by tanning, post-tanning, and finishing operations (i.e., wet blue trimmings, chrome splits, shavings, buffing dust, crust trimming wastes). This review article shows that there is considerable bioenergy potential for the use of leather solid wastes as a green fuel. The biogas potential of leather solid wastes is estimated to be 40,532.9 m 3 /day whereas the biodiesel potential is estimated as 15,452.6 L/day. The bio-oil and bio-char potential of leather solid wastes is estimated to be 80,513.0 L/day and 45.8 tons/day, respectively. Several factors influence the biofuel process efficacy, which needs to be taken into consideration while setting up a biofuel recovery plant. The overall biofuel potential of leather solid wastes shows that this feedstock is an untapped resource for energy recovery to add commercial benefits to India's energy supply. Furthermore, in addition to the economic benefits for investors, the use of leather solid wastes for biofuel production will yield a positive environmental impact., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Perturbation-based balance training (PBT) improves reactive stepping in older adults and people with neurological disorders. Slip-induced falls are a threat to older adults, leading to hip fractures. Fall-prone individuals must be trained to regain balance during a fall in the posterolateral direction. This study aims to analyze the characteristics of the reactive step induced by a laterally inclined platform. This cross-sectional study included 46 healthy participants who performed a "lean and release" backward fall using a platform with two inclined angles on each side. Kinovea software was used to analyze the step width. Reactive steps, characterized by crossover or medial foot placement, are preventive measures against posterolateral falls. The first objective was on the narrowed step width that was subjected to analysis using analysis of variance (ANOVA) and Tukey's post hoc assessment, indicating a tendency toward posterolateral falls. As part of our second objective, the inclined platform resulted in uneven loading between the legs, with a preference for the unloaded leg as the reactive leg ( p < 0.001), as determined by Fisher's exact test and Cramer's V. These characteristics align closely with those observed in modified constraint-induced movement therapy (mCIMT). The angled platform had a significant effect on selecting the reactive leg, particularly at higher angles ( p < 0.001). Thus, the study suggested that the device is capable of inducing posterolateral falls and exhibited mCIMT characteristics., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2023 Jaison Jacob Mathunny et al.)

Large-scale representative source apportionment studies are uncommon, undermining source contribution studies in India, particularly in high-altitude locations. Kodaikanal is a high-altitude region in India's Western Ghats, with spatial heterogeneity of sources altering chemical complexity; thus, the associated implications are unknown. We conducted the campaign study REBER (Research on Equivalent Black Carbon Monitoring in an Eco-sensitive Region) at three Kodaikanal sites to understand local point sources, characteristics, and distribution of eBC during the winter-to-summer monsoon transition. For two main reasons: to understand the seasonal change of BC since the transition period has the lowest wind speeds and the highest particulate concentrations and is prone to high pollution events most often during seasonal transition months, and to study local pollution since the meridional monsoon and zonal winds in study region weaken whereby the transport of pollutants from ocean to land and vice versa is minimal. The results showed that the eBC mass concentration was 85% higher than in the previous study conducted by Bhaskar et al. (2018) during the monsoon transition period. To determine the ratio of fossil fuel and wood-burning sources, a real-time apportionment model of atmospheric eBC is used. The percentage of wood burning in the background location ranges from 21.12 to 88.98%. Wood burning leads in residential sites with 57.5 ± 7.3%, whereas fossil fuel contribution dominates traffic sites with 69.84 ± 10.2%. Fossil fuel contributions are significant in different characteristics of environments, ranging from 42.5 to 69.84%. The results of the conditional bivariate probability function (CBPF) analysis pointed out a competition between anthropogenic and natural sources to contribute as local sources to the monitoring stations. A scanning electron microscope (SEM) paired with an energy dispersive X-ray (EDX) analysis found that the particle size was 93% relatively large compared to other hill stations in India. The variation in the chemical constituents indicates that the particles originated from various sources., (© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

The case report describes the details of a man in his 40s admitted for evaluation of renal failure. Biochemical testing revealed parathyroid hormone (PTH) -independent hypercalcaemia. The evaluations for the usual causes such as malignancies, granulomatous diseases, multiple myeloma and vitamin D toxicity were negative. Fluorodeoxyglucose Positron Emission Tomography (FDG-PET) scan identified diffuse uptake in the muscles, and the subsequent muscle biopsy showed non-caseating granulomas suggestive of granulomatous myositis, possibly sarcoidosis, in view of raised ACE levels and the absence of other causes. The patient showed a dramatic response to glucocorticoids, with prompt relief of symptoms and normalisation of serum calcium and creatinine. The case highlights the importance of considering sarcoid myositis in the evaluation of hypercalcaemia and the need for prompt initiation of glucocorticoid therapy to achieve favourable outcomes. The successful use of FDG-PET in diagnosing PTH-independent hypercalcaemia suggests its potential as a valuable tool in the diagnostic algorithm for this condition., Competing Interests: Competing interests: None declared., (© BMJ Publishing Group Limited 2023. No commercial re-use. See rights and permissions. Published by BMJ.)

Competing Interests: Competing interests: None declared.

Purpose: To evaluate the clinical effectiveness and health economic benefits of a novel indwelling lattice-based device for fecal management in bedridden patients., Materials and Methods: This nonrandomized, two-arm study included 70 bedridden patients (≥18 years exhibiting liquid stool) referred from the ICU of surgery and medicine units of a 2000-bed tertiary care referral hospital, assigned to the intervention and control groups. About 35 patients were eligible to be included in the intervention group while 35 patients with contraindications to the intervention device were included in the usual care control group. Assessments were made before and every 24 hours during the study, and all patients were closely monitored for development of incontinence-associated dermatitis (IAD) and hospital-acquired pressure injury., Results: The test device was successfully deployed on the first attempt and effectively diverted fecal matter in all 35 patients, with no adverse events. In the control group, 83% of the patients developed IAD, which resulted in prolonged hospitalization and increased expenses. Overall, the control group (with adult diapers) required greater time, resources, and efforts for fecal management and resulted in increased patient morbidity., Conclusion: The patient management time, resource consumption, overall cost of hospital admission, and the complication rates are significantly lower with the use of the novel lattice-based device than with the use of adult diapers for fecal management., How to Cite This Article: Sheth H, Rao S, Karthik V. Clinical and Health Economic Evaluation of a Novel Device for Fecal Management in Bedridden Patients. Indian J Crit Care Med 2023;27(10):759-765., Competing Interests: Source of support: The test devices used in the study are provided by Consure Medical, New Delhi, India Conflict of interest: None, (Copyright © 2023; The Author(s).)

Parathyroid hormone acts through its receptor, PTHR1, expressed on osteoblasts, to control bone remodeling. Metabolic flexibility for energy generation has been demonstrated in several cell types dependent on substrate availability. Recent studies have identified a critical role for PTH in regulating glucose, fatty acid and amino acid metabolism thus stimulating both glycolysis and oxidative phosphorylation. Therefore, we postulated that PTH stimulates increased energetic output by osteoblasts either by increasing glycolysis or oxidative phosphorylation depending on substrate availability. To test this hypothesis, undifferentiated and differentiated MC3T3E1C4 calvarial pre-osteoblasts were treated with PTH to study osteoblast bioenergetics in the presence of exogenous glucose. Significant increases in glycolysis with acute ∼1 h PTH treatment with minimal effects on oxidative phosphorylation in undifferentiated MC3T3E1C4 in the presence of exogenous glucose were observed. In differentiated cells, the increased glycolysis observed with acute PTH was completely blocked by pretreatment with a Glut1 inhibitor (BAY-876) resulting in a compensatory increase in oxidative phosphorylation. We then tested the effect of PTH on the function of complexes I and II of the mitochondrial electron transport chain in the absence of glycolysis. Utilizing a novel cell plasma membrane permeability mitochondrial (PMP) assay, in combination with complex I and II specific substrates, slight but significant increases in basal and maximal oxygen consumption rates with 24 h PTH treatment in undifferentiated MC3T3E1C4 cells were noted. Taken together, our data demonstrate for the first time that PTH stimulates both increases in glycolysis and the function of the electron transport chain, particularly complexes I and II, during high energy demands in osteoblasts., Competing Interests: The authors declare that there is no conflict of interest., (© 2023 The Authors.)

Rapid and effective methods for the detection of analytes such as water contaminants, food adulterants and biomolecules are essential for the protection of public health and environmental protection. Most of the currently established analytical techniques need sophisticated equipment, centralized testing facilities, costly operations, and trained personnel. Such limitations make them inaccessible to the general populace, particularly in regions with limited resources. The emergence of microfluidic devices offers a promising alternative to overcome several such constraints. This work describes a protocol for fabricating a low-cost, open-source paper-based microfluidic device using easily available tools and materials for colorimetric detection of analytes. The ease and simplicity of fabrication allow users to design customized devices. The device is coupled with an imaging box assembled from 3D printed parts to maintain uniform lighting conditions during analytical testing. The platform allows digital imaging using smartphones or cameras to instantaneously capture images of reaction zones on the device for quantitative analysis. The system is demonstrated for detecting hexavalent chromium, a toxic water contaminant. The image analysis is performed using open-source ImageJ for quantification of results. The approach demonstrated in this work can be readily adopted for a wide range of sensing applications., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Author(s).)

A zirconium(IV)-based metal-organic framework (MOF) fluorophore containing the 2,5-diaminoterephthalic acid (H 2 BDC-(NH) 2 ) linker was synthesized and characterized. The physicochemically stable, porous ( S BET = 504 m 2 g -1 ) MOF ( 1' ) exhibited selective and sensitive fluorescence turn-on behavior toward the sodium dodecyl sulfate (SDS) surfactant and turn-off response toward vitamin B 12 . This is the first ever reported MOF-based dual optical sensor of SDS and vitamin B 12 . Other competitive analytes did not interfere in the detection of both the analytes. Along with the lowest ever reported limit of detection (LOD) values (LOD for SDS = 108 nM and LOD for vitamin B 12 = 45.3 nM), 1' displayed short response time for SDS (50 s) and vitamin B 12 (5 s) detection. The MOF was able to detect SDS in various real water samples and vitamin B 12 in various bio-fluids (urine and serum) and pH media. A MOF-coated cotton composite was fabricated, which displayed a visible color change under UV light even after treating it with a nanomolar concentration of both the analytes. The sensor displayed excellent reusability up to five cycles of sensing. Various experimental outcomes evidenced that the electrostatic interaction between the -NH 2 groups of the linker and the -SO 3 - group of SDS is the possible reason for the selective SDS sensing. For vitamin B 12 , the energy transfer from the probe to vitamin B 12 resulted in fluorescence quenching. In addition, the catalytic performance of 1' was investigated in the condensation reaction between benzaldehyde derivatives with cyanoacetamide with high yields in ethanol at 70 °C. The solid was used for three cycles with no decrease in its activity and selectivity. PXRD and FESEM analysis before and after the reaction suggested the retention of the crystallinity of 1' , thus indicating catalyst stability.

Background: According to Indian TB report 2020, 66,225 MDR/RR-TB cases were detected in India, 56,569 (85%) were put on treatment, and 40,397 (75%) were initiated on shorter drug regimens at the time of diagnosis. In the absence of an effective vaccine, there is an urgent need for new treatment regimens, drugs, and diagnostics to slow the evolution of drug resistance and limit transmission of resistant variants, as well as to ameliorate the treatment outcome of patients infected with MDR/XDR M. tuberculosis strains., Aim: To evaluate the efficacy of a shorter drug regimen in MDR-PTB and estimate the adverse effects of drugs used in the regimen., Methods: This is an institution-based prospective study which included 135 confirmed MDR-PTB patients. Patients with extra-pulmonary MDR-TB and use of SLI for more than one month were excluded., Results: The success rate using a shorter regimen was 65.2% which is respectable, given the COVID-19 pandemic considered during the study period. Minor adverse events such as nausea (39.3%) and vomiting (34.8%) were reported. Rare adverse effects such as hearing loss (8.9%) and hypothyroidism (0.2%) were also seen in the study population., Conclusion: Overall treatment success was similar when compared to other studies done previously. A shorter drug regimen was associated with minor adverse effects such as gastrointestinal adverse effects such as vomiting and hearing loss observed in elderly patients. Baseline unknown drug resistance and lower BMI were associated with unsuccessful outcomes. Measures should be taken to improve nutrition. Our results argue the need for improving baseline DST at peripheral areas in order to effectively evaluate resistance to other drugs, especially in settings with high levels of first and second-line drug resistance., Competing Interests: There are no conflicts of interest., (Copyright: © 2023 Journal of Family Medicine and Primary Care.)

The purpose of the review is to evaluate wearable sensor placement, their impact and validation of wearable sensors on analyzing gait, primarily the postural instability in people with stroke. Databases, namely PubMed, Cochrane, SpringerLink, and IEEE Xplore were searched to identify related articles published since January 2005. The authors have selected the articles by considering patient characteristics, intervention details, and outcome measurements by following the priorly set inclusion and exclusion criteria. From a total of 1077 articles, 142 were included in this study and classified into functional fields, namely postural stability (PS) assessments, physical activity monitoring (PA), gait pattern classification (GPC), and foot drop correction (FDC). The review covers the types of wearable sensors, their placement, and their performance in terms of reliability and validity. When employing a single wearable sensor, the pelvis and foot were the most used locations for detecting gait asymmetry and kinetic parameters, respectively. Multiple Inertial Measurement Units placed at different body parts were effectively used to estimate postural stability and gait pattern. This review article has compared results of placement of sensors at different locations helping researchers and clinicians to identify the best possible placement for sensors to measure specific kinematic and kinetic parameters in persons with stroke.

Topical minoxidil is used for treating different hair disorders. Even though it is an effective therapy, many patients show poor compliance due to the cost, side effects, and duration of treatment. Topical minoxidil is the mainstay treatment for androgenetic alopecia (AGA). Recently, low alcohol or alcohol-free topical minoxidil formulation has proven to be an alternative for patients suffering from AGA, including those with poor compliance with other therapies. Thus, the current article provides the positioning of low alcohol or alcohol-free topical minoxidil to manage AGA in Indian clinical practice., Competing Interests: Satish Udare, Anita Barua, Anurag Mathur, Dayanand TR, Kapil Jain, Manas Puhan, Pawan Bajaj, Richa Sharma, Sanjay Gamit, V Ramesh, V Venugopal and Vignesh Karthik are members of the advisory board of Dr. Reddy's Laboratories. Monil Yogesh Neena Gala, Snehal Muchhala and Amey Mane are employees of Dr. Reddy's Laboratories., (Copyright: © 2023 International Journal of Trichology.)

Evolving Anthropocene epoch wields significant influence in altering atmospheric carbon, which affects the carbon cycle, leading to climate change. Understanding the carbon stock, fate, and transport across ecosystems are essential in determining India's carbon budget, hitherto, unavailable. In this study, we have analysed the stock, source, distribution, flux, and the relationship between terrestrial and aquatic black carbon over a high-altitude mountainous area in the Western Ghats region using the data collected from September 2019 to February 2021. Soil Organic Carbon (SOC) and Black Carbon (BC) are the highest in the forest region (SOC:23 ± 3 g of C/kg (dry weight (dw)), BC:6 ± 3 g/kg) and are the lowest in the urban region (SOC: 13 ± 2 g of C/kg (dw), BC:2 ± 1 g/kg). SOC is labile, whereas BC is non-labile. The BC/SOC ratio represents soil carbon lability. Topsoil BC/SOC ratios vary by land use and land cover, with urban areas having greater labile carbon pools than the forests. Dissolved BC (DBC) concentrations were most strongly correlated with bulk Dissolved Organic Carbon (DOC) concentrations in midstream (R = 0.6, p < 0.05), headwater streams (R = 0.3, p < 0.05) and to the soil bulk DBC (R = 0.3, p < 0.05), indicating the presence of transfer mechanism of soil to streams. The molecular associations revealed the presence of biolabile autochthonous compounds suggesting the crucial role land use and land cover play on watersheds. A positive relationship between DOC with seasonal hydrology and gradient significantly influences the DBC flux across regional streams. Intercomparison of observed terrestrial and aquatic carbon stocks with globally modelled data indicates an overestimation of regional-scale stock. These new findings have repercussions to policy framework on regional climate change. Further, the results suggest that a consistent quantification of BC and integration of regional, and global source-to-sink process are needed in order to understand and better quantify biogeochemical process cycles and associated climatic impacts., (Copyright © 2022 Elsevier B.V. All rights reserved.)

In the latest times, considerable studies have been performed closer to detecting emerging pollutant such as paracetamol in wastewater. Electrochemical sensor developments have recently started to determine in fewer concentrations effectively. The detection of paracetamol using standard protocols corresponding to electroanalytical techniques has a greater impact noticed in directing the detecting process toward biosensors. Non-enzymatic sensors are the peak of all electro analysis approaches. Functionalized materials, such as metal oxide nanoparticles, conducting polymers, and carbon-based materials for electrode surface functionalization have been used to create a fortification for distributing passive enzyme-free biosensors. Synergic effects are possible by enhancing loading capacity and mass transfer of reactants for attaining high analytical sensitivity using a variety of nanomaterials with large surface areas. The main focus of this study is to address the prevailing issues in the identification of paracetamol with the tasks in the non-enzymatic sensors field, followed by the useful methods of electro analysis studies., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Black Carbon (BC) and Organic Carbon (OC) are the principal chemical aerosol components generated during combustion, both of which play a key role in air pollution, human health and climate change. Several studies of OC and BC have been conducted over India to assess the contribution from household and fossil fuel-based sources; however, studies on their emissions and their contribution from forest and cropland fires are quite limited. To address this issue, as part of this research, we derived a vegetation burning-based inventory of BC and OC aerosols over India at a resolution of 250 m × 250 m. Using a consumed biomass technique, we estimated emissions based on updated emission factor estimates. During the fire season in India (March-June), the mean OC and BC emissions were 2.1 ± 5.2 × 10 13  kg per year and 1.8 ± 4.4 × 10 12  kg per year, respectively. Andhra Pradesh had the highest total carbonaceous aerosol emissions during the study period. Forest fires were prevalent in the northeastern states, while agricultural fires were prevalent in Gujarat, Chhattisgarh, Odisha, Madhya Pradesh, Bihar, Tripura, Uttar Pradesh, and Andhra Pradesh. The previous inventory, conducted at a coarser resolution (25 km × 25 km), overestimated open burning by 5 Mt. Our results were highly correlated with global bottom-up model values, especially the Fire Inventory (FINN). Our analysis showed that vegetative burning contributed 80.32% of the total carbon stock, with agricultural burning being the largest source of vegetative burning. Based on these findings, measures and strategies to control agricultural burning which would reduce significantly the total emissions of BC and OC with implications to improvement in air quality, human health and climate should be planned., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Retinitis pigmentosa (RP) is the most common cause of hereditary blindness, and may occur in isolation as a non-syndromic condition or alongside other features in a syndromic presentation. Biallelic or monoallelic mutations in one of eight genes encoding pre-mRNA splicing factors are associated with non-syndromic RP. The molecular mechanism of disease remains incompletely understood, limiting opportunities for targeted treatment. Here we use CRISPR and base edited PRPF6 and PRPF31 mutant cell lines, and publicly-available data from human PRPF31 +/- patient derived retinal organoids and PRPF31 siRNA-treated organotypic retinal cultures to confirm an enrichment of differential splicing of microtubule, centrosomal, cilium and DNA damage response pathway genes in these cells. We show that genes with microtubule/centrosome/centriole/cilium gene ontology terms are enriched for weak 3' and 5' splice sites, and that subtle defects in spliceosome activity predominantly affect efficiency of splicing of these exons. We suggest that the primary defect in PRPF6 or PRPF31 mutant cells is microtubule and centrosomal defects, leading to defects in cilium and mitotic spindle stability, with the latter leading to DNA damage, triggering differential splicing of DNA damage response genes to activate this pathway. Finally, we expand understanding of "splicing factor RP" by investigating the function of TTLL3 , one of the most statistically differentially expressed genes in PRPF6 and PRPF31 mutant cells. We identify that TTLL3 is the only tubulin glycylase expressed in the human retina, essential for monoglycylation of microtubules of the cilium, including the retinal photoreceptor cilium, to prevent cilium degeneration and retinal degeneration. Our preliminary data suggest that rescue of tubulin glycylation through overexpression of TTLL3 is sufficient to rescue cilium number in PRPF6 and PRPF31 mutant cells, suggesting that this defect underlies the cellular defect and may represent a potential target for therapeutic intervention in this group of disorders., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Nazlamova, Villa Vasquez, Lord, Karthik, Cheung, Lakowski and Wheway.)

The increasing trend of industrialization leads to tremendous release of industrial effluents. Waste water treatment is one of the important sectors to focus in order to overcome the most threatening issue of waste disposal and to ensure sustainability. Sustainable and energy efficient treatment methods are the attractive technologies for their current implementation of waste management. Even though the existing technologies are effective, unsustainability makes them unfit for their extended applications. Conventional and advanced technologies have been extensively implemented for the treatment of wide spectrum of effluents. Hybrid technologies including chemical and biological methods also emerging as promising technologies but secondary sludge generation is still unaddressed. Even though effectiveness of biochar varies over type of contaminants, cost-effectiveness and eco-friendly nature extended their applications in waste management. Nanotechnology and membrane technology are the promising and emerging areas of interest due to their widespread applications in waste water treatment. Carbon nano structures, nano filters, graphene, nano magnets modified with activated carbon are the potential candidates for the treatment. The present review demonstrates the emerging treatment technologies with special focus to nano based waste water treatment methods., (Copyright © 2022. Published by Elsevier Ltd.)

Recently, a decrease in water availability and quality has been raised due to rapid industrialization, unsustainable agricultural activities and anthropogenic activities. Heavy metals are considered significant pollutants in the water environment, cause environmental hazards and health effects to humans. For monitoring water contaminants utilized different conventional techniques. Still, they have some drawbacks, such as cost expensive, ecological issues, and processing time, requiring technicians and researchers to operate them effectively. Biosensors have become reasonable devices for screening and identifying environmental contaminants because of their different benefits contrasted with other detecting techniques. This review summarizes the toxic effect of heavy metal and their source, occurrence. A detailed discussion is provided on the heavy metal recognition materials for detecting heavy metals in wastewater. Lab on chip (LOC) is an emerging micro-electrical mechanical system (MEMS) device that intakes liquid and makes it move through the micro-channels, to accomplish fast, cost-effective and profoundly sensitive analysis with significant yield. LOC also provided a discussion on numerous laboratory functions on a single platform. This article attempts to discuss the detection of heavy metals using lab on a chip by suitable recognition materials. Further, the design and fabrication mechanism and their recognition abilities of LOC were also reviewed. The review mainly focuses on the application of LOC biosensors, pros, and cons, and suggests a roadmap towards future development to enhance the practical use in pollutant monitoring., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

It has been estimated that more than 390 million people are infected with Dengue virus every year; around 96 millions of these infections result in clinical pathologies. To date, there is only one licensed viral vector-based Dengue virus vaccine CYD-TDV approved for use in dengue endemic areas. While initially approved for administration independent of serostatus, the current guidance only recommends the use of this vaccine for seropositive individuals. Therefore, there is a critical need for investigating the influence of Dengue virus serostatus and immunological mechanisms that influence vaccine outcome. Here, we provide comprehensive evaluation of sero-status and host immune factors that correlate with robust immune responses to a Dengue virus vector based tetravalent vaccine (TV003) in a Phase II clinical cohort of human participants. We observed that sero-positive individuals demonstrate a much stronger immune response to the TV003 vaccine. Our multi-layered immune profiling revealed that sero-positive subjects have increased baseline/pre-vaccination frequencies of circulating T follicular helper (cTfh) cells and the Tfh related chemokine CXCL13/BLC. Importantly, this baseline/pre-vaccination cTfh profile correlated with the vaccinees' ability to launch neutralizing antibody response against all four sero-types of Dengue virus, an important endpoint for Dengue vaccine clinical trials. Overall, we provide novel insights into the favorable cTfh related immune status that persists in Dengue virus sero-positive individuals that correlate with their ability to mount robust vaccine specific immune responses. Such detailed interrogation of cTfh cell biology in the context of clinical vaccinology will help uncover mechanisms and targets for favorable immuno-modulatory agents., Competing Interests: The authors have declared that no competing interests exist.

In this present study, a novel and low cost surface improved material was prepared from the farm waste material (Borassus flabellifer male inflorescence) and its surface was enhanced by the sulphuric acid treatment to intensify the Ni(II) ions adsorption. The adsorption individualities such as availability of functional groups, essential elements and the exterior side and structural properties of the material were assessed by the FT-IR, EDX, SEM and XRD investigation. The impact of varied adsorption influencing parameters on Ni(II) ions adsorption was studied and optimized as pH - 6.0, biosorbent dosage - 1.5 g/L, contact time - 60 min and temperature - 303 K via batch adsorption examination. Modeling examinations were carried with varied adsorption isotherm (Langmuir, Freundlich, Fritz-Schlunder and Temkin) and kinetic models (Pseudo-first order, Pseudo-second order and Elovich kinetics). Thermodynamic studies were carried out at varied Ni(II) ions concentrations (25 mg/L - 150 mg/L) and temperatures (303 K-333 K) to explain the nature of Ni(II) ions adsorption on Borassus flabellifer male inflorescence. The prepared material has shown the most suitable Ni(II) ions adsorption results for the Langmuir isotherm (R 2  = 0.9808) and Pseudo-first order kinetic models (R 2  = 0.9735 for 25 mg/L). Thereby, the modeling study revealed that the prepared material has received the Ni(II) ions adsorption capacity (q m ) value of 20.31 mg/g and the Ni(II) ions adsorption was physisorption. Thermodynamic results demonstrated that the Ni(II) ions adsorption was immediate, exothermic and favorable at low temperature., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Herein, we aim to evaluate the photodetector performance of various nanostructured materials (thin films, 2-D nanolayers, 1-D nanowires, and 0-D quantum dots) in ultraviolet (UV), visible, and infrared (IR) regions. Specifically, semiconductor-based metal oxides such as ZnO, Ga 2 O 3 , SnO 2 , TiO 2 , and WO 3 are the majority preferred materials for UV photodetection due to their broad band gap, stability, and relatively simple fabrication processes. Whereas, the graphene-based hetero- and nano-structured composites are considered as prominent visible light active photodetectors. Interestingly, graphene exhibits broad band spectral absorption and ultra-high mobility, which derives graphene as a suitable candidate for visible detector. Further, due to the very low absorption rate of graphene (2%), various materials have been integrated with graphene (rGO-CZS, PQD-rGO, N-SLG, and GO doped PbI 2 ). In the case of IR photodetectors, quantum dot IR detectors prevails significant advantage over the quantum well IR detectors due to the 0-D quantum confinement and ability to absorb the light with any polarization. In such a way, we discussed the most recent developments on IR detectors using InAs and PbS quantum dot nanostructures. Overall, this review gives clear view on the development of suitable device architecture under prominent nanostructures to tune the photodetector performance from UV to IR spectral regions for wide-band photodetectors., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Purpose of Review: In this review, we provide a recent update on bioenergetic pathways in osteocytes and identify potential future areas of research interest. Studies have identified a role for regulation of bone formation and bone resorption through osteocyte mechanosensing and osteocyte secreted factors. Nevertheless, there is a paucity of studies on the bioenergetics and energy metabolism of osteocytes, which are required for the regulation of bone remodeling., Recent Findings: Osteocytes are cells of the osteoblast lineage embedded in bone. The osteocyte lacunocanalicular network within the skeletal matrix is exposed to a unique hypoxic environment. Therefore, the bioenergetic requirements of these cells could differ from other bone cells due to its location in the ossified matrix and its role in bone regulation transduced by mechanical signals. Recent findings highlighted in this review provide some evidence that metabolism of these cells is dependent on their location due to the substrates present in the microenvironment and metabolic cues from stress pathways. Both glycolysis (glucose metabolism) and oxidative phosphorylation (mitochondrial dynamics, ROS generation) affect osteocyte function and viability. In this review, we provide evidence that is currently available about information regarding bioenergetics pathways in osteocytes. We discuss published studies showing a role for hypoxia-driven glucose metabolism in regulating osteocyte bioenergetics. We also provide information on various substrates that osteocytes could utilize to fuel energetic needs, namely pyruvate, amino acids, and fatty acids. This is based on some preliminary experimental evidence that is available in literature. The role of parathyroid hormone PTH and parathryoid hormone-related peptide PTHrP in bone anabolism and resorption, along with regulation of metabolic pathways in the cells of the skeletal niche, needs to be explored further. Mitochondrial metabolism has a role in osteocyte bioenergetics through substrate utilization, location of the osteocyte in the bone cortex, and mitochondrial biogenesis. While there are limitations in studying metabolic flux in traditional cell lines, there are now novel cell lines and sophisticated tools available to study osteocyte bioenergetics to help harness its potential in vivo in the future., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

ORF7a is an accessory protein common to SARS-CoV1 and the recently discovered SARS-CoV2, which is causing the COVID-19 pandemic. The ORF7a protein has a structural homology with ICAM-1 which binds to the T lymphocyte integrin receptor LFA-1. As COVID-19 has a strong immune component as part of the disease, we sought to determine whether SARS-CoV2 would have a similar structural interaction with LFA-1. Using molecular docking simulations, we found that SARS-CoV2 ORF7a has the key structural determinants required to bind LFA-1 but also the related leukocyte integrin Mac-1, which is also known to be expressed by macrophages. Our study shows that SARS-CoV2 ORF7a protein has a conserved Ig immunoglobulin-like fold containing an integrin binding site that provides a mechanistic hypothesis for SARS-CoV2's interaction with the human immune system. This suggests that experimental investigation of ORF7a-mediated effects on immune cells such as T lymphocytes and macrophages (leukocytes) could help understand the disease further and develop effective treatments., (© 2021 The Author(s).)

Pomegranate peel, a major waste from the food processing industries containing biologically active compounds, could be converted into value-added products having medicinal properties. Present study deals with the ultrasound-assisted surfactant, namely dimethyl sulfoxide (DMSO) aided polyphenolics extraction from pomegranate peel waste using double distilled water (DDW) as a solvent. Maximum total yield of extraction and total polyphenolic content (TPC) were found respectively to be 43.58 ± 1.0 and 49.55 ± 0.8%, at optimized sonication parameters viz. temperature 50 °C, power density 1.2 W/mL and time 40 min followed by surfactant aided extraction under optimum conditions 0.6% DMSO, 50 °C and 150 rpm for 90 min. Kinetic models were developed to determine the polyphenolics concentration and validated. GC-MS analysis of the extract revealed 22 phenolic compounds. Thus, the acquired results have ensured the significance of ultrasound pre-treated surfactant aided extraction of polyphenolic compounds and this process can be developed for commercial production., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Microbial production of xylanase is gaining the commercial importance, due to its wide range of applications from paper and pulp to food and feed industries. Streptomyces geysiriensis was used for the production of extracellular xylanase from lignocellulosic substrates such as rice bran and saw dust, under solid-state fermentation. The influence of pH, temperature and incubation period for the maximum production of xylanase was investigated with 1:2 (w/v) of substrate to moisture ratio at 100 rpm shaking conditions. The maximum production was recorded after 5 days of fermentation with pH 8.0 at 40 °C. The scale-up was done based on the results of optimized parameters using 3 L Applikon autoclavable bioreactor with maximum yield of 186 U/ml after 4 days of fermentation. Extracellular xylanase was separated by partitioning in aqueous two-phase system consisting of 20% polyethylene glycol 6000 and 12% K 2 HPO 4 with maximum yield of 93.97%. The investigation of the effect of pH and temperature and its incubation time showed that xylanase was retained its activity in a pH range of 6.5-8.5, with thermal stability from 20 °C to 60 °C up to 180 min. The presence of metal ions was found to inhibit the activity of xylanase especially Cu 2+ and Zn 2+ . Xylanase was stable both at 4 °C and room temperature (35 °C) for 30 and 9 days respectively. The kinetic parameters K m (0.48 mg/ml) and V max (8.33 U/mg) were determined using birchwood xylan as substrate., (Copyright © 2020 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)

With the increasing use of 18 F-fluorodeoxyglucose (FDG) positron emission tomography-computed tomography (PET-CT) scans in oncology, the finding of thyroid incidentalomas, also popularly described as PET-associated incidental neoplasms (PAINs) of the thyroid gland is not unusual. The 18 F-FDG PET-CT scans of all patients who underwent imaging for indications other than thyroid malignancy at our tertiary care center between January 1 and December 31, 2017, were retrospectively reviewed for PAINs of the thyroid. A total of 1737 18 F-FDG PET-CT scans were done at our center in the year 2017. 288 thyroid incidentalomas were detected in the said period; the rate of PET-CT-detected thyroid incidentalomas being 16.58%, focal incidentalomas among them being 11.7%. Only 29 out of 204 patients (14.21%) with focal thyroid incidentalomas in our cohort underwent an aspiration cytology and/or ultrasound. The rate of malignancy among the PET detected focal thyroid incidentalomas in the cohort of patients with a proven diagnosis was 10.34%. Our study highlights the challenges in the evaluation and management of PAIN in a tertiary care oncology setting. None of the factors studied including nodule size and standardized uptake value predicted the risk of malignancy. Clinicians specializing in the management of thyroid nodules need to understand the clinical significance of the PAIN, and we hope that our unique experience adds to the limited clinical information available in this regard., Competing Interests: There are no conflicts of interest., (Copyright: © 2020 World Journal of Nuclear Medicine.)

Polymer based solid electrolytes (SEs) are envisaged as futuristic components of safer solid state energy devices. But the semi-crystalline nature and slow dynamics of the host polymer matrix are found to hamper the ion transport through the solid polymer network and hence solid state devices are still far beyond the scope of practical application. In this study, we unravel the synergistic roles of Li salt (LiClO 4 ) and two different polymers - polyethylene oxide (PEO) and polydimethyl siloxane (PDMS), in the Li ion transport through their solid blend based electrolyte. A detailed study using dielectric spectroscopy and thermo-mechanical analysis is conducted to understand the tunability of the PEO chain dynamics with LiClO 4 and the mechanism of hopping of Li ions by forming ion pairs with oxygen dipoles on the PEO backbone is established. Despite the lack of PDMS's capability to solvate ions and promote ion transport directly, its proper mixing within the PEO host matrix is demonstrated to enhance ion transport due to the influence of PDMS on the segmental dynamics of PEO. A detailed molecular dynamics study supported by experimental validation suggests that even inert polymers can affect the dynamics of the active host matrix and increase ion transport, leading to next generation high ionic conductivity solid matrices, and opens new avenues in designing polymer based transparent electrolytes., Competing Interests: There is no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Wearable electronics have become the norm over the last few years. Extensive use of wearable wireless devices (WWD) in greater proximity to the body has increased concern about potential biological effects due to the interaction of human tissues with electromagnetic (EM) fields. Human tissues absorb radiofrequency (RF) waves that are capable of affecting energy states at a molecular level, leading to unsafe effects. Remarkably, thermal effect due to the absorption of RF waves is a better biological manifestation than traditional specific absorption rate (SAR) values. In this study, we investigated the application of infrared thermography (IRT) to obtain temperature dynamics and reconstructed average SAR to evaluate the exposure compliance of WWDs. A microstrip-based monopole antenna was used to determine the biological effects of the interaction of EM waves with the body. SAR was obtained using EM field simulations and through reconstruction from thermal measurements with the use of bio-heat equations. The maximum average SAR on the skin was 50 mWkg -1 for the simulations and 54 mWkg -1 from reconstruction after IRT experiments. The maximum temperature change in both cases was observed to be less than 1 °C. The difference between the average SAR obtained through IRT and simulation tools was 8.9% on average. SAR determination from the information acquired using IR temperature dynamics can assess bio-compliance of modern wearable devices for various wireless applications. Bioelectromagnetics. 39:451-459, 2018. © 2018 Wiley Periodicals, Inc., (© 2018 Wiley Periodicals, Inc.)

Atomically thin materials such as graphene are uniquely responsive to charge transfer from adjacent materials, making them ideal charge-transport layers in phototransistor devices. Effective implementation of organic semiconductors as a photoactive layer would open up a multitude of applications in biomimetic circuitry and ultra-broadband imaging but polycrystalline and amorphous thin films have shown inferior performance compared to inorganic semiconductors. Here, the long-range order in rubrene single crystals is utilized to engineer organic-semiconductor-graphene phototransistors surpassing previously reported photogating efficiencies by one order of magnitude. Phototransistors based upon these interfaces are spectrally selective to visible wavelengths and, through photoconductive gain mechanisms, achieve responsivity as large as 10 7 A W -1 and a detectivity of 9 × 10 11 Jones at room temperature. These findings point toward implementing low-cost, flexible materials for amplified imaging at ultralow light levels., (© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Background and Aims: Evidence-based medicine (EBM) is defined as the use of scientifically proven evidence for delivering best possible health care to patients. Despite growing emphasis on the need for EBM-based practice, acceptability, and perceptions toward EBM might differ among health professionals. The objective of this study was to assess the attitude, knowledge, and current practices of EBM among perioperative care health professionals in India., Material and Methods: This was a single point paper-based questionnaire survey carried out in February 2014 among delegates registered for an EBM conference on "perioperative care" held at a Tertiary referral Cancer Centre in India. Participation was voluntary and respondents were given the option of remaining anonymous., Results: Out of 190 questionnaires, 123 (65%) were returned. Most respondents (98%) agreed that practicing EBM improved patient care. The need to follow departmental protocols (22%) worries about the cost of implementing new treatments (20%) and inadequate skills to critically appraise articles (16%) accounted for major barriers in implementing EBM in clinical practice, with only 15% of respondents stating reluctance to change set practice. "Randomized controlled trial" and "number needed to treat" were the best and least understood EBM terms. Regarding awareness of 10 commonly used EBM-based guidelines in perioperative medicine, the percentage of correct responses ranged from 20% to 88%., Conclusion: Although most respondents agreed that practicing EBM improved patient care, many of them showed a low level of awareness regarding fundamental aspects of EBM. In addition to encouraging implementation of EBM, there should be increased focus on training in EBM methods., Competing Interests: There are no conflicts of interest.

Background: A departmental audit in March 2015 revealed significant mortality rate of 40% in blunt chest trauma patients (much greater than the global 25%). A study was thus planned to study morbidity and predictors of mortality in blunt chest trauma patients admitted to our hospital., Methods: This study was a prospective observational study of 139 patients with a history of blunt chest trauma between June 2015 and November 2015 after the Institutional Ethics Committee approval in April 2015. The sample size was calculated from the prevalence rate in our institute from the past medical records., Results: The morbidity factors following blunt chest injuries apart from pain were need for Intensive Care Unit stay, mechanical ventilation, and pneumonia/acute respiratory distress syndrome. Significant predictors of mortality in our study were SpO 2 <80 at the time of presentation, Glasgow coma scale ≤ 8, patients with four or more rib fractures, presence of associated head injury, Injury Severity Score >16, and need for mechanical ventilation. By calculating the likelihood ratios of each respiratory sign, a clinical score was devised., Conclusion: The modifiable factors affecting morbidity and mortality were identified. Mild to moderate chest injury due to blunt trauma is difficult to diagnose. The restoration of respiratory physiology has not only significant implications on recovery from chest injury but also all other injuries. It is our sincere hope that the score we have formulated will help reduce mortality and morbidity after further trials., Competing Interests: There are no conflicts of interest.

Graphene oxide (GO) resistive memories offer the promise of low-cost environmentally sustainable fabrication, high mechanical flexibility and high optical transparency, making them ideally suited to future flexible and transparent electronics applications. However, the dimensional and temporal scalability of GO memories, i.e., how small they can be made and how fast they can be switched, is an area that has received scant attention. Moreover, a plethora of GO resistive switching characteristics and mechanisms has been reported in the literature, sometimes leading to a confusing and conflicting picture. Consequently, the potential for graphene oxide to deliver high-performance memories operating on nanometer length and nanosecond time scales is currently unknown. Here we address such shortcomings, presenting not only the smallest (50 nm), fastest (sub-5 ns), thinnest (8 nm) GO-based memory devices produced to date, but also demonstrate that our approach provides easily accessible multilevel (4-level, 2-bit per cell) storage capabilities along with excellent endurance and retention performance-all on both rigid and flexible substrates. Via comprehensive experimental characterizations backed-up by detailed atomistic simulations, we also show that the resistive switching mechanism in our Pt/GO/Ti/Pt devices is driven by redox reactions in the interfacial region between the top (Ti) electrode and the GO layer.

The studies on the biocontrol potential of pink pigmented facultative methylotrophic (PPFM) bacteria other than the genus Methylobacterium are scarce. In the present study, we report three facultative methylotrophic isolates; PPO-1, PPT-1, and PPB-1, respectively, identified as Delftia lacustris, Bacillus subtilis , and Bacillus cereus by 16S rRNA gene sequence analysis. Hemolytic activity was tested to investigate the potential pathogenicity of isolates to plants and humans, the results indicates that the isolates PPO-1, PPT-1, and PPB-1 are not pathogenic strains. Under in vitro conditions, D. lacustris PPO-1, B . subtilis PPT-1, and B . cereus PPB-1 showed direct antagonistic effect by inhibiting the mycelial growth of fungal pathogens; Fusarium oxysporum f. sp. lycopersici (2.15, 2.05, and 1.95 cm), Sclerotium rolfsii (2.14, 2.04, and 1.94 cm), Pythium ultimum (2.12, 2.02, and 1.92 cm), and Rhizoctonia solani (2.18, 2.08, and 1.98 cm) and also produced volatile inhibitory compounds. Under plant growth chamber condition methylotrophic bacterial isolates; D . lacustris PPO-1, B . subtilis PPT-1, and B. cereus PPB-1 significantly reduced the disease incidence of tomato. Under greenhouse condition, D . lacustris PPO-1, B . subtilis PPT-1, and B . cereus PPB-1 inoculated tomato plants, when challenged with F . oxysporum f. sp. lycopersici, S . rolfsii, P . ultimum , and R . solani , increased the pathogenesis related proteins (β-1,3-glucanase and chitinase) and defense enzymes (phenylalanine ammonia lyase, peroxidase, polyphenol oxidase, and catalase) on day 5 after inoculation. In the current study, we first report the facultative methylotrophy in pink pigmented D. lacustris, B . subtilis , and B . cereus and their antagonistic potential against fungal pathogens. Direct antagonistic and ISR effects of these isolates against fungal pathogens of tomato evidenced their possible use as a biocontrol agent.

Preservation of native knee anatomy may confer improved patient satisfaction, as suggested by patient satisfaction scores in unicondylar versus total knee replacement. Bicompartmental knee replacement (BKR) implants similarly promote native tissue preservation. We retrospectively reviewed 42 consecutive patients who underwent BKR from 2006 to 2007. Outcome measures were evaluated. At an average follow-up of 103 months (range 87-110), 34/42 (81%) of implants survived. Among the retained implants, the Knee Society Score (KSS) grade was excellent in 26/34 (76.5%), good in 5/34 (14.7%), fair in 3/34 (8.8%), and poor in 0/34 (0%) of cases. Midterm results of BKR demonstrated 81% survival and 76% with excellent KSS grading. Despite a 20% revision rate at the short-term follow-up, the retained implants functioned well at the midterm follow-up.