1. A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs.
- Author
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Decaro N, Elia G, Martella V, Desario C, Campolo M, Trani LD, Tarsitano E, Tempesta M, and Buonavoglia C
- Subjects
- Animals, DNA, Viral chemistry, DNA, Viral genetics, Diarrhea diagnosis, Diarrhea virology, Dog Diseases diagnosis, Dogs, Feces virology, Hemagglutination Inhibition Tests veterinary, Parvoviridae Infections diagnosis, Parvoviridae Infections virology, Parvovirus, Canine isolation & purification, Polymerase Chain Reaction methods, Sensitivity and Specificity, Taq Polymerase metabolism, Diarrhea veterinary, Dog Diseases virology, Parvoviridae Infections veterinary, Parvovirus, Canine genetics, Polymerase Chain Reaction veterinary
- Abstract
We describe a rapid, sensitive and reproducible real-time PCR assay for detecting and quantifying canine parvovirus type 2 (CPV-2) DNA in the feces of dogs with diarrhea. An exogenous internal control was added to control the assay performance from extraction to amplification. The method was demonstrated to be highly specific and sensitive, allowing a precise CPV-2 DNA quantitation over a range of eight orders of magnitude (from 10(2) to 10(9) copies of standard DNA). The reproducibility of the CPV-2 real-time PCR assay was assessed by calculating the coefficients of variation (CV) intra-assay and inter-assay for samples containing amounts of CPV-2 DNA spanning the whole range of the real-time PCR standard curve. Then, fecal specimens from diarrheic dogs were analyzed by hemagglutination (HA), conventional PCR and real-time amplification. Comparison between these different techniques revealed that real-time PCR is more sensitive than HA and conventional gel-based PCR, allowing to detect low viral titers of CPV-2 in infected dogs.
- Published
- 2005
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