1. Detection of Salmonella in finishing pigs on farm and at slaughter in Piedmont, Italy.
- Author
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Lomonaco S, Decastelli L, Bianchi DM, Nucera D, Grassi MA, Sperone V, and Civera T
- Subjects
- Animals, Bacteriophage Typing, Cross-Sectional Studies, Drug Resistance, Bacterial, Feces microbiology, Humans, Italy epidemiology, Meat microbiology, Microbial Sensitivity Tests, Palatine Tonsil microbiology, Polymerase Chain Reaction, Salmonella classification, Salmonella drug effects, Salmonella Food Poisoning epidemiology, Salmonella Food Poisoning prevention & control, Salmonella Infections, Animal microbiology, Salmonella enterica classification, Salmonella enterica drug effects, Salmonella enterica isolation & purification, Serotyping, Swine, Swine Diseases microbiology, Abattoirs, Animal Husbandry methods, Salmonella isolation & purification, Salmonella Infections, Animal epidemiology, Swine Diseases epidemiology
- Abstract
Salmonella is one of the most common causes of human gastroenteritis often associated with pork consumption. The aims of this cross-sectional study were to collect preliminary data on the presence of Salmonella enterica in pigs in Piedmont (Italy), through sampling on farm and at slaughter and to gather pilot data on serotypes and phagetypes present in the sampled area and distribution of anti-microbial resistance among isolated strains. Salmonella was detected through culture and identified with Salmonella spp. and Salmonella Typhimurium PCR; positive samples were serotyped, phagetyped and tested for antibiotic susceptibility. Positive samples (from 9% of faeces up to 29% of tonsils) were found in 64% of the herds. Salmonella spp. was retrieved also from scalding water. Most of the isolates were Salmonella Derby, Salmonella Typhimurium and Salmonella 4,5,12:i:-. The results of Salmonella Typhimurium specific PCR suggested that Salmonella 4,5,12:i:- might be unrecognized by serotyping. Anti-microbial resistance was recorded in 75-100% of the isolates. Phagetyping allowed the identification of DT104B and DT46A strains. These results set the bases for further research studies that would aim to estimate the real herd prevalence in Piedmont and the diffusion of serotypes and anti-microbial resistant strains within the same region.
- Published
- 2009
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