1. Beneficial effect of an omega-6 PUFA-rich diet in non-steroidal anti-inflammatory drug-induced mucosal damage in the murine small intestine.
- Author
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Ueda T, Hokari R, Higashiyama M, Yasutake Y, Maruta K, Kurihara C, Tomita K, Komoto S, Okada Y, Watanabe C, Usui S, Nagao S, and Miura S
- Subjects
- Animals, Blood Platelets drug effects, Blood Platelets immunology, Blood Platelets metabolism, Cell Adhesion drug effects, Cell Adhesion Molecules genetics, Cell Adhesion Molecules metabolism, Chemotaxis, Leukocyte drug effects, Fish Oils administration & dosage, Fish Oils toxicity, Intestinal Mucosa immunology, Intestinal Mucosa metabolism, Intestinal Mucosa pathology, Intestine, Small immunology, Intestine, Small metabolism, Intestine, Small pathology, Leukocytes drug effects, Leukocytes immunology, Leukocytes metabolism, Male, Meat Products toxicity, Mice, Inbred C57BL, Microvessels drug effects, Microvessels immunology, Microvessels metabolism, RNA, Messenger metabolism, Time Factors, Anti-Inflammatory Agents, Non-Steroidal toxicity, Diet, Fatty Acids, Omega-6 administration & dosage, Indomethacin toxicity, Intestinal Mucosa blood supply, Intestinal Mucosa drug effects, Intestine, Small blood supply, Intestine, Small drug effects, Safflower Oil administration & dosage
- Abstract
Aim: To investigate the effect of a fat rich diet on non-steroidal anti-inflammatory drug (NSAID)-induced mucosal damage in the murine small intestine., Methods: C57BL6 mice were fed 4 types of diets with or without indomethacin. One group was fed standard laboratory chow. The other groups were fed a fat diet consisting of 8% w/w fat, beef tallow (rich in SFA), fish oil, (rich in omega-3 PUFA), or safflower oil (rich in omega-6 PUFA). Indomethacin (3 mg/kg) was injected intraperitoneally from day 8 to day 10. On day 11, intestines and adhesions to submucosal microvessels were examined., Results: In the indomethacin-treated groups, mucosal damage was exacerbated by diets containing beef tallow and fish oil, and was accompanied by leukocyte infiltration (P < 0.05). The mucosal damage induced by indomethacin was significantly lower in mice fed the safflower oil diet than in mice fed the beef tallow or fish oil diet (P < 0.05). Indomethacin increased monocyte and platelet migration to the intestinal mucosa, whereas safflower oil significantly decreased monocyte and platelet recruitment (P < 0.05)., Conclusion: A diet rich in SFA and omega-3 PUFA exacerbated NSAID-induced small intestinal damage via increased leukocyte infiltration. Importantly, a diet rich in omega-6-PUFA did not aggravate inflammation as monocyte migration was blocked.
- Published
- 2015
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