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Objective: The aim of this study is to evaluate the effects of a seat-integrated mobilization system on motion activity, muscle stiffness and discomfort during prolonged driving., Method: During a 4.5-h driving task, motion activity, muscle stiffness and discomfort (measured subjectively and objectively) of long-haul truck drivers were determined and compared intra-individually in a test condition with mobilization (seat-integrated stimulation) and a control condition under standardized conditions in a simulated driving study (N = 16)., Results: In the experimental condition, participants showed a significantly increased motion activity and a reduced muscle stiffness compared to the control condition. Furthermore, discomfort occurred significantly more frequently in the control condition., Conclusion: The mobilization system increased motion activity and reduced discomfort as well as muscle stiffness. Therefore, it provides considerable potential to counteract negative effects of prolonged sitting and to promote truck driver's health., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: The study was supported by the Mercedes-Benz AG., (Copyright © 2022 Elsevier Ltd. All rights reserved.)