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This article presents the results of research that focused on the nutrition and related health issues of medieval and early modern dogs found in the territory of present-day Lithuania. In this study, we present bone collagen carbon (δ 13 C) and nitrogen (δ 15 N) isotope ratios for seventy-five dogs recovered from seven sites which were dated back to the between the 12th and 18th C AD. In addition, by studying the remains of almost 200 dogs, we were able to estimate changes in the sizes and morphotypes of canines across over 600 years. On the basis of stable isotope and historical data, as well as the osteometric analysis, we discuss the dietary patterns of different sizes and types of dogs from the sites related to different social strata and time periods. The results of our study demonstrate that the size, type, diet, and health of canines from different time periods and sociocultural environments varied. Overall, carbon isotopic signals indicate that dogs' diets were based on C 3 plant environment foods (cereals and animals), while freshwater fish was more important for some individuals in coastal Klaipėda/Memelburg Castle. The stable isotope analysis supported the historical records, indicating that cereals were highly important in the diet of elite dogs. Meanwhile, urban dogs had a different nutrition. In the Middle Ages, the consumption of plant-based foods was likely higher compared to the early modern period. Our study also revealed that the diets of dogs did not correlate with individual size. Compared to pigs, dogs had a higher intake of animal foods in their diet. In general, the nutrition of the studied canines was similar to that of the rural human population of the same period.

In agricultural habitats, diets and trophic positions of syntopic granivorous small mammals are not known sufficiently. Agroecosystems may be quite complex isotopically and the most complex situation concerns the nitrogen-15 isotope as δ 15 N values are influenced by many internal and external fluxes. We analysed the isotopic niches of striped field ( Apodemus agrarius ), yellow-necked ( Apodemus flavicollis ), and harvest ( Micromys minutus ) mice living sympatrically and syntopically in apple and plum orchards, raspberry and currant plantations, and nearby meadows that were used as control habitats. Carbon ( δ 13 C) and nitrogen ( δ 15 N) stable isotope ratios from hair samples were used as a proxy for their diet. As the main hypothesis, we expected differences in the isotopic niches of these three species, minimising interspecific competition. All species were fully separated according to δ 15 N values, presuming different amounts of food of animal origin in their diet. The separation of species according to δ 13 C was not expressed in all habitats. The core dietary niches of these species were fully separated in the apple orchards and raspberry plantations. Intraspecific differences of the isotopic niche were not present in any of the three species: that is, resources were equally used by males and females, adults, subadults, and juveniles.

Diets and trophic positions of co-occurring animals are fundamental issues in their ecology, and these issues in syntopic rodents have been studied insufficiently. Using carbon ( δ 13 C) and nitrogen ( δ 15 N) stable isotope ratios from hair samples, we analysed the trophic niches of common ( Microtus arvalis ), field ( M. agrestis ), and root ( M. oeconomus ) voles co-occurring in orchards, berry plantations, and nearby meadows (as control habitat to orchards and plantations). We tested if the niche of the dominant common vole was the widest, whether its width depended on the presence of other vole species, and whether there were intraspecific differences. Results suggest stability in the trophic niches of all three Microtus species, as season explained only 2% of the variance. The widest trophic niche was a characteristic of the dominant common vole, the range of δ 13 C values exceeding the other two species by 1.6, the range of δ 15 N values exceeding the other two species by 1.9, and the total area of niche exceeding that of the other voles by 2.3-3 times. In the meadows and apple orchards, co-occurring vole species were separated according to δ 13 C (highest values in the dominant common vole), but they maintained similar δ 15 N values. Results give new insights into the trophic ecology small herbivores, showing the impact of species co-occurrence.

Coastal residents are quite often expected to consume a significant amount of aquatic resources, though historical evidence often reveals a rather complex diet. To better understand the actual consumption and the distribution of various foods, stable isotope (δ 13 C and δ 15 N) analyses were employed to skeletal remains from three coastal communities, Palanga, Kretinga and Smeltė, ranging in date from the medieval period to the early modern ages (14 th -early 20 th c.) near the Curonian Lagoon and the Baltic Sea in Lithuania. Animal bones from the region, covering the same time periods, were also analysed. Stable isotope results were compared with historical records. According to historical sources different types of diet were prevalent during that period of time: Medieval Prussian-Lithuanian peasant, Lithuanian fisherman, German urban, and religious-based. Elevated δ 15 N values for Smeltė samples suggest a diet consisting of considerable amounts of freshwater fish protein, which is in contrast to historical sources. There were no significant differences in stable isotope values between males and females, while subadult δ 15 N values were significantly higher than adult ones, indicating that those children were breastfed for an extended period. Meanwhile, Palanga and Kretinga samples had isotope values suggesting a high reliance on terrestrial resources and a peasant type of diet.

Meadows in river deltas are characterized by a high diversity and abundance of small mammals. However, neither their spatial arrangement nor differences in their use of microhabitat can necessarily explain the dense co-occurrence of sympatric species. We investigated how several small mammal species share a seasonally flooded meadow of limited size, testing predictions (P1) that herbivore, granivore, insectivore, and omnivore species are separated in time (dominant in different years), (P2) that sympatric species undergo isotopic partitioning, and (P3) that there are intraspecific differences in diet. Stable carbon and nitrogen isotope signatures in the hair of seven synantropic shrew, vole, and mice species were used as a proxy for their diet. We found that the three most abundant species in eight of the nine years were from different diet groups. However, based on the number of species in the functional groups, the state of small mammal community was considered unfavored in five out of the nine investigation years. In years with the greatest dominance of Apodemus agrarius , the small mammal community was characterized by decreased diversity and Micromys minutus was either in low abundance or absent. In 2014 and 2016, years of low abundance or absence of M. oeconomus , M. agrestis, and M. glareolus were both recorded in high numbers. Differences in the isotopic signatures of the three most abundant small mammal species in the community were clearly expressed and core areas in the isotopic space were separated, showing their dependence on different dietary resources. Intraspecific dietary separation between young and adult animals was observed only in M. oeconomus . Thus, the high species diversity of small mammals and the formation of their community in this investigated flooded meadow are maintained by isotopic partitioning (segregation in dietary space) and by changes in their number over time (shifting dominance)., Competing Interests: None declared.

Various studies have shown that stable isotope analysis has the potential to verify the geographic origin of foods and drinks. However, stable isotope composition is not always constant in the environment and can even change in the same area. Dairy products are of particular interest as a group of foods that play an important role in feeding the population. The composition of milk is fundamentally dependent on the feeding of the cows, and thereby on a particular environment. To better understand the amount of variation in δ 18 O, δ 13 C, and δ 15 N values in the milk from the same area, we measured stable isotope ratios in cow milk water, artesian water, and precipitation (δ 18 O) as well as in bulk milk samples (δ 13 C and δ 15 N) collected in 2014⁻2016. Different water and food sources were available during the winter (artesian water only and dry grass) and summer (artesian water and fresh grass), and spring and autumn seasons reflected transitional periods. Oxygen stable isotope ratios in milk water were relatively lower in winter and transitional seasons and higher in summer, showing the dependence on the main water source. δ 13 C values reflected particular food sources. This study shows the applicability of the stable isotope ratio method in linking cow milk to specific environments and reveals the amount of variation in stable isotope ratios in the same area. These results could be valuable for other studies on geographical origin determination of dairy products.

Objectives: The Baltic region, particularly Lithuania, was a politically vibrant area of Eastern Europe during the medieval and early modern period. To better understand the diet of Lithuanians during the late 14th to early 18th century, we examine stable carbon and nitrogen isotope ratios from bone and dentin samples from the site of Alytus. We investigate possible dietary differences based on sex, age, and religious practice, as well as dietary changes throughout an individual's lifetime, within the broader European milieu., Materials and Methods: Stable carbon and nitrogen isotope analysis was conducted on bone (n = 35) and dentin (n = 38) collagen samples from a total of 39 individuals buried in the cemetery at Alytus (late 14th to early 18th centuries)., Results: Results indicate individuals at Alytus consumed a C 3 terrestrial based diet. The δ 13 C and δ 15 N values are not significantly different between bone and dentin, and did not vary by sex., Discussion: The diet at Alytus was homogeneous between males and females and between tissue types. The lack of evidence indicating substantial consumption of fish is unexpected given widespread Catholic fasting practices and marine resource trade throughout Europe. Comparisons with other populations indicate that individuals from Alytus differ in diet from contemporaneous Polish-Lithuanian Commonwealth elites. Comparison of the diets of non-elite individuals in the Eastern Baltic region also reveals dietary variability., (© 2018 Wiley Periodicals, Inc.)

With the arrival of the Early Neolithic Globular Amphora and Corded Ware cultures into the southeastern Baltic, ca. 2900/2800-2400 cal BC, a new type of economy was introduced, animal husbandry. However, the degree to which this transformed the subsistence economy is unknown. Here, we conducted organic residue analyses of 64 ceramic vessels to identify their contents. The vessels were sampled from 10 Lithuanian archaeological sites dating across the Subneolithic-Neolithic transition to the Early Bronze Age (ca. 2900/2800-1300 cal BC). Our results demonstrate that regardless of location or vessel type, many ceramics were used to process aquatic resources. Against our expectations, this association continued even after marked economic change concurrent with the migration of pastoralists from central and southeastern Europe, as evidenced by recent ancient DNA analysis of human remains. Moreover, we observed dairy fats in pottery from all cultures of the Early Neolithic (i.e. Rzucewo, Globular Amphora and Corded Ware) but unlike other regions of Europe, it seems that these were incorporated into indigenous culinary practices. Furthermore, some vessels were used to process plant foods, and others may have been used for the production and/or storage of birch bark tar. However, evidence for domesticated plant processing, for example millet, was absent. We show that organic residue analysis of pottery provides a different picture of past consumption patterns compared to the stable isotope analysis of human remains from isolated burials where a clear dietary shift is evident., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest., (© The Author(s) 2019.)

In the present study, a combination of the stable carbon isotope ratio ( 13 C/ 12 C) with radiocarbon data ( 14 C) allowed us to perform the aerosol source apportionment. Filter samples of PM 1 were collected during the warm and cold periods in rural and urban sites in Lithuania. The 14 C/ 12 C ratio of total carbon (TC) was measured using the single stage accelerator mass spectrometer quantifying of fossil and non-fossil derived aerosol emissions. The δ 13 C value was measured using an elemental analyser interfaced with an isotope ratio mass spectrometer. We have found that the highest fraction of contemporary carbon (f c  = 0.82) was measured during a warm period in a rural location. A higher fraction of fossil fuel-derived carbon was observed for air masses transported from highly industrialized Western European regions during both seasons. Isotope mass balance calculations revealed that the traffic emissions composed 15 and 25 % in rural and urban sites, respectively, and did not change during either season. Input from coal-derived aerosol particles was estimated to be 15 % at an urban site during the cold period. The combination of the stable carbon isotope ratio with the radiocarbon data allowed us to distinguish coal, liquid fossil fuel combustion, and non-fossil derived aerosol particle emissions.

Stable isotope analysis was applied to describe the poultry house environment. The poultry house indoor environment was selected for this study due to the relevant health problems in animals and their caretakers. Air quality parameters including temperature, relative humidity, airflow rate, NH 3 , CO 2 and total suspended particles, as well as mean levels of total airborne bacteria and fungi count, were measured. Carbon isotope ratios ( 13 C/ 12 C) were obtained in size-segregated aerosol particles. The carbon ( 13 C/ 12 C) and nitrogen ( 15 N/ 14 N) isotope ratios were measured in feed, litter, scrapings from the ventilation system, feathers and eggs. Additionally, the distribution of δ 13 C and δ 15 N values in different tissues of the chicken was examined. The airborne bacteria and fungi extracted from the air filters collected from poultry farms were grown in the laboratory in media with known isotope values and measured for stable isotope ratios. Analysis of isotope fractionation between microorganisms and their media indicated the applicability of stable isotope analysis in bulk samples for the identification of source material. The analysed examples imply that stable isotope analysis can be used to examine the indoor environment along with its biology and ecology, and serve as an informative bioanalytical tool.

Studying the isotopic composition of the hair of two rodent species trapped in the territories of Great Cormorant colonies, we aimed to show that Great Cormorants transfer biogens from aquatic ecosystems to terrestrial ecosystems, and that these substances reach small mammals through the trophic cascade, thus influencing the nutrient balance in the terrestrial ecosystem. Analysis of δ(13)C and δ(15)N was performed on two dominant species of small mammals, Apodemus flavicollis and Myodes glareolus, inhabiting the territories of the colonies. For both species, the values of δ(13)C and δ(15)N were higher in the animals trapped in the territories of the colonies than those in control territories. In the hair of A. flavicollis and M. glareolus, the highest values of δ(15)N (16.31±3.01‰ and 17.86±2.76‰, respectively) were determined in those animals trapped in the biggest Great Cormorant colony. δ(15)N values were age dependent, highest in adult A. flavicollis and M. glareolus and lowest in juvenile animals. For δ(13)C values, age-dependent differences were not registered. δ(15)N values in both small mammal species from the biggest Great Cormorant colony show direct dependence on the intensity of influence. Biogenic pollution is at its strongest in the territories of the colonies with nests, significantly diminishing in the ecotones of the colonies and further in the control zones, where the influence of birds is negligible. Thus, Great Cormorant colonies alter ecosystem functioning by enrichment with biogens, with stable isotope values in small mammals significantly higher in the affected territories., (Copyright © 2016 Elsevier B.V. All rights reserved.)