1. Predictive potential of various plasma inflammation-, angiogenesis-, and extracellular matrix remodeling-associated mediators for intra-amniotic inflammation and/or microbial invasion of the amniotic cavity in preterm labor.
- Author
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Joo E, Hong S, Park KH, Kim HJ, Lee MJ, and Shin S
- Subjects
- Humans, Female, Pregnancy, Retrospective Studies, Adult, Calgranulin A blood, Endostatins blood, Acute-Phase Proteins analysis, Interleukin-6 blood, Amniocentesis, Serum Amyloid P-Component analysis, Serum Amyloid P-Component metabolism, Haptoglobins analysis, Haptoglobins metabolism, Vascular Endothelial Growth Factor Receptor-1 blood, Predictive Value of Tests, Extracellular Matrix metabolism, Angiogenesis, Calgranulin B, Obstetric Labor, Premature microbiology, Obstetric Labor, Premature blood, Amniotic Fluid microbiology, Amniotic Fluid metabolism, Matrix Metalloproteinase 8 blood, C-Reactive Protein analysis, C-Reactive Protein metabolism, Biomarkers blood, Chorioamnionitis microbiology, Chorioamnionitis blood, Insulin-Like Growth Factor Binding Protein 2 blood, Matrix Metalloproteinase 2 blood
- Abstract
Purpose: To determine whether various inflammatory-, angiogenic/anti-angiogenic-, and extracellular matrix remodeling-associated proteins in plasma, alone or in combination with conventional blood-based markers, can predict intra-amniotic inflammation and/or microbial invasion of the amniotic cavity (IAI/MIAC) in women with spontaneous preterm labor (PTL)., Methods: A total of 193 singleton pregnant women with PTL (23-33 weeks) were included in this retrospective cohort study. Plasma samples were obtained at the time of amniocentesis. Amniotic fluid (AF) was cultured for microorganism detection and consequent MIAC diagnosis. IL-6 levels were determined in AF and used to identify IAI (AF IL-6 ≥ 2.6 ng/mL). Endostatin, haptoglobin, IGFBP-2/3, LBP, M-CSF, MMP-2/8, pentraxin 3, PlGF, S100A8/A9, and VEGFR-1 levels were assayed in plasma samples by ELISA. CRP levels and neutrophil-to-lymphocyte ratio (NLR) were measured., Results: Plasma LBP, MMP-8, and S100A8/A9 levels, CRP levels, and NLR were significantly higher, and plasma IGFBP-2 and MMP-2 levels were significantly lower in women with IAI/MIAC than in those without this condition, whereas no baseline variables differed significantly between the two groups. Using a stepwise regression analysis, a noninvasive prediction model for IAI/MIAC was developed, which included plasma LBP, MMP-2, and MMP-8 levels (area under the curve [AUC], 0.785). The AUC for this prediction model was significantly or borderline greater than that of any single factor included in the model., Conclusions: IGFBP-2, LBP, MMP-2, MMP-8, and S100A8/A9 may represent valuable plasma biomarkers for predicting IAI/MIAC in women with PTL. Combination of LBP, MMP-2, and MMP-8 expression data can significantly improve the predictive potential for IAI/MIAC., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2024
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