1. Glucose-1-phosphate thymidylyltransferase promotes the production of 3-O-α-mycarosylerythronolide B in Streptomyces coelicolor.
- Author
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Gao H, Langer S, Larson T, Gregory MA, and Smith MCM
- Subjects
- Nucleotidyltransferases genetics, Nucleotidyltransferases metabolism, Bacterial Proteins genetics, Bacterial Proteins metabolism, Saccharopolyspora genetics, Saccharopolyspora metabolism, Saccharopolyspora enzymology, Anti-Bacterial Agents biosynthesis, Streptomyces coelicolor genetics, Streptomyces coelicolor enzymology, Streptomyces coelicolor metabolism, Erythromycin analogs & derivatives
- Abstract
Aims: The main objective of this study was to produce erythronolide B (EB) and 3-O-α-mycarosylerythronolide B (MEB) in Streptomyces coelicolor and enhance the MEB production by expressing the glucose-1-phosphate thymidylyltransferase (RfbA)., Methods and Results: We expressed eryF and eryB genes (eryBII, eryBIII, eryBIV, eryBV, eryBVI, and eryBVII) to produce EB and MEB. The expression was confirmed by quantitative real-time polymerase chain reaction. Furthermore, the MEB's production was improved by more than 100-fold by expressing an enzyme, RfbA, which is absent from the erythromycin gene cluster, to promote the biosynthesis of TDP-L-mycarose. We discuss the feasibility of alternative Streptomyces species for erythromycin production based on the presence or absence of RfbA., Conclusions: The RbfA enzyme from Saccharopolyspora erythraea was expressed in S. coelicolor M1152 along with the MEB biosynthesis pathway, resulting in a large increase in MEB production (>100-fold)., (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
- Published
- 2024
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