Search

The enhanced catalytic properties of bimetallic nanoparticles have been extensively investigated. In this study, bimetallic Ag-M (M = Au, Pt, or Pd) cotton fabrics were fabricated using a combination of electroless deposition and galvanic replacement reactions, and improvement in their peroxidase-mimicking catalytic activity compared to that of the parent Ag fabric was studied. The Ag-Pt bimetallic nanozyme fabric, which showed the highest catalytic activity and ability to simultaneously generate hydroxyl (•OH) and superoxide (O 2 •- ) radicals, was assessed as a urine glucose sensor. This nanozyme fabric sensor could directly detect urinary glucose in the pathophysiologically relevant high millimolar range without requiring sample predilution. The sensor could achieve performance on par with that of the current clinical gold standard assay. These features of the Ag-Pt nanozyme sensor, particularly its ability to avoid interference effects from complex urinary matrices, position it as a viable candidate for point-of-care urinary glucose monitoring., (© 2024. The Author(s).)

This paper, offers a new method for simulating variable-order fractional differential operators with numerous types of fractional derivatives, such as the Caputo derivative, the Caputo-Fabrizio derivative, the Atangana-Baleanu fractal and fractional derivative, and the Atangana-Baleanu Caputo derivative via power-law kernels. Modeling chaotical systems and nonlinear fractional differential equations can be accomplished with the utilization of variable-order differential operators. The computational structures are based on the fractional calculus and Newton's polynomial interpolation. These methods are applied to different variable-order fractional derivatives for Wang-Sun, Rucklidge, and Rikitake systems. We illustrate this novel approach's significance and effectiveness through numerical examples., (© 2024. The Author(s).)

As the amount and complexity of biomedical data continue to increase, machine learning methods are becoming a popular tool in creating prediction models for the underlying biomedical processes. Although all machine learning methods aim to fit models to data, the methodologies used can vary greatly and may seem daunting at first. A comprehensive review of various machine learning algorithms per biomedical applications is presented. The key concepts of machine learning are supervised and unsupervised learning, feature selection, and evaluation metrics. Technical insights on the major machine learning methods such as decision trees, random forests, support vector machines, and k-nearest neighbors are analyzed. Next, the dimensionality reduction methods like principal component analysis and t-distributed stochastic neighbor embedding methods, and their applications in biomedical data analysis were reviewed. Moreover, in biomedical applications predominantly feedforward neural networks, convolutional neural networks, and recurrent neural networks are utilized. In addition, the identification of emerging directions in machine learning methodology will serve as a useful reference for individuals involved in biomedical research, clinical practice, and related professions who are interested in understanding and applying machine learning algorithms in their research or practice., (© 2024. The Author(s) under exclusive licence to Biomedical Engineering Society.)

Faults in photovoltaic (PV) modules may occur due to various environmental and physical factors. To prevent faults and minimize investment losses, fault diagnosis is crucial to ensure uninterrupted power production, extended operational lifespan, and a high level of safety in PV modules. Recent advancements in inspection techniques and instrumentation have significantly reduced the cost and time required for inspections. A novel stacking-based ensemble approach was performed in the present study for the accurate classification of PV module visible faults. The present study utilizes AlexNet (a pre-trained network) to extract image features from the aerial images of PV modules with the aid of MATLAB software. Furthermore, J48 algorithm was applied to perform the feature selection task to determine the most relevant features. The features derived as output from the J48 algorithm were passed onto train eight base classifiers namely, Naïve Bayes, logistic regression (LR), J48, random forest (RF), multilayer perceptron (MLP), logistic model tree (LMT), support vector machines (SVM) and k-nearest neighbors (kNN). The best performing five classifiers on the front run with higher classification accuracies were selected to formulate three categories of stacking ensemble groups as follows: (i) three-class ensemble (SVM, kNN, and LMT), (ii) four-class ensemble (SVM, kNN, LMT, and RF), and (iii) five-class ensemble (SVM, kNN, LMT, RF, and MLP). A comparison in the performance of the aforementioned stacked ensembles was evaluated with different meta classifiers. The obtained results infer that the four-class stacking ensemble model (SVM, kNN, LMT, and RF) with RF as the predictor achieved the highest possible classification accuracy of 99.04%., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Platelets are known for their indispensable role in hemostasis and thrombosis. However, alteration in platelet function due to oxidative stress is known to mediate various health complications, including cardiovascular diseases and other health complications. To date, several synthetic molecules have displayed antiplatelet activity; however, their uses are associated with bleeding and other adverse effects. The commercially available curcumin is generally a mixture of three curcuminoids: curcumin, demethoxycurcumin, and bisdemethoxycurcumin. Although crude curcumin is known to inhibit platelet aggregation, the effect of purified curcumin on platelet apoptosis, activation, and aggregation remains unclear. Therefore, in this study, curcumin was purified from a crude curcumin mixture and the effects of this preparation on the oxidative stress-induced platelet apoptosis and activation was evaluated. 2,2'-Azobis(2-methylpropionamidine) dihydrochloride (AAPH) compound was used as an inducer of oxidative stress. Purified curcumin restored AAPH-induced platelet apoptotic markers like reactive oxygen species, intracellular calcium level, mitochondrial membrane potential, cardiolipin peroxidation, cytochrome c release from mitochondria to the cytosol, and phosphatidyl serine externalization. Further, it inhibited the agonist-induced platelet activation and aggregation, demonstrating its antiplatelet activity. Western blot analysis confirms protective effect of the purified curcumin against oxidative stress-induced platelet apoptosis and activation via downregulation of MAPKs protein activation, including ASK1, JNK, and p-38. Together, these results suggest that the purified curcumin could be a potential therapeutic bioactive molecule to treat the oxidative stress-induced platelet activation, apoptosis, and associated complications.

In the present work, acidic (direct) and basic precipitation (indirect) methods were used to demonstrate the influence of the mode of precipitation on the structural properties of ZnO nanoparticles (NPs). Four samples of ZnO nanoparticles were prepared, two samples via each mode of precipitation. DZOa and IZOa were the aged samples prepared via acidic and basic precipitation methods, and DZOwa and IZOwa were processed without aging. Both precipitation processes were carried out without using any surfactant reagents. Zinc hydroxide precipitate, which was formed during the basic precipitation method, could be critical in deciding the properties of ZnO NPs, unlike zinc hydroxide formed during acidic precipitation. Aging of zinc hydroxide, synthesised by basic precipitation method for 48 hours was found to be an added advantage in controlling the properties of ZnO NPs. The influence of the mode of precipitation on the structural properties and antibacterial activity of ZnO NPs against Gram-positive and Gram-negative bacterial strains was tested. The antibacterial activity of all four ZnO NPs was analysed via zone of inhibition measurements at a concentration dose of 200 μg ml -1 . IZOa nanoparticles prepared using the basic precipitation method showed a higher antibacterial activity against three Gram-negative and one Gram-positive strains, namely, Klebsiella pneumoniae , Pseudomonas aeruginosa , Staphylococcus aureus , and Escherichia coli . DZOa nanoparticles synthesized through acidic precipitation showed relatively high antibacterial activity against Salmonella typhimurium , a Gram-negative strain. ZnO NPs prepared without aging, IZOwa and DZOwa, showed a higher antibacterial activity against E. coli and Bacillus sp. strains, respectively. All ZnO NPs were characterized via UV-visible, FTIR, XRD, and HRSEM techniques.

While conducting field trial of 82 genotypes of bottle gourd at Delhi during 2020-2021, a particular genotype, IC-0262269 was found to be affected by chlorotic curly stunt disease (CCSD). The affected plants were severely stunted and bearing very small chlorotic and crinkle leaves. The disease incidence in the said genotype was as high as 80% among different replicated trial blocks. The application of PCR using a generic primers specific to begomoviruses, as well as species-specific PCR diagnostics to six tomato-infecting begomoviruses: tomato leaf curl New Delhi virus (ToLCNDV), tomato leaf curl Palampur virus (ToLCPalV), tomato leaf curl Joydebpur virus (ToLCJoV), tomato leaf curl Gujrat virus (ToLCGuV), tomato leaf curl Bangalore virus (ToLCBV), and chilli leaf curl virus (ChiLCV) showed that, only ToLCPalV could be detected in the genotype IC-0262269. Following, rolling circle amplification, cloning and sequencing of full-length DNA-A and DNA-B genome of an isolate BoG1-ND from the genotype IC-0262269 revealed association of ToLCPalV with the disease. The successful agro-infection of the cloned genome of BoG1-ND (DNA-A and DNA-B) in the plants of Nicotiana benthamiana and bottle gourd demonstrated that ToLCPalV is the causal begomovirus of CCSD. The study provides the first evidence of the natural occurrence of ToLCPalV in bottle gourd crop and also showed that the bottle gourd genotype IC-0262269 is super-susceptible to ToLCPalV., Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03838-y., Competing Interests: Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. We are declaring that to the best of our knowledge, there are no conflict of interest existing in the content of the manuscript., (© King Abdulaziz City for Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

Malaria, a highly perilous infectious disease, impacted approximately 230 million individuals globally in 2019. Mosquitoes, vectors of over 10% of worldwide diseases, pose a significant public health menace. The pressing need for novel antimalarial drugs arises due to the imminent threat faced by nearly 40% of the global population and the escalating resistance of parasites to current treatments. This study comprehensively addresses prevalent parasitic and viral illnesses transmitted by mosquitoes, leading to the annual symptomatic infections of 400 million individuals, placing 100 million at constant risk of contracting these diseases. Extensive investigations underscore the pivotal role of traditional plants as rich sources for pioneering pharmaceuticals. The latter half of this century witnessed the ascent of bioactive compounds within traditional medicine, laying the foundation for modern therapeutic breakthroughs. Herbal medicine, notably influential in underdeveloped or developing nations, remains an essential healthcare resource. Traditional Indian medical systems such as Ayurveda, Siddha, and Unani, with a history of successful outcomes, highlight the potential of these methodologies. Current scrutiny of Indian medicinal herbs reveals their promise as cutting-edge drug reservoirs. The propensity of plant-derived compounds to interact with biological receptors positions them as prime candidates for drug development. Yet, a comprehensive perspective is crucial. While this study underscores the promise of plant-based compounds as therapeutic agents against malaria and dengue fever, acknowledging the intricate complexities of drug development and the challenges therein are imperative. The journey from traditional remedies to contemporary medical applications is multifaceted and warrants prudent consideration. This research aspires to offer invaluable insights into the management of malaria and dengue fever. By unveiling plant-based compounds with potential antimalarial and antiviral properties, this study aims to contribute to disease control. In pursuit of this goal, a thorough understanding of the mechanistic foundations of traditional antimalarial and antidengue plants opens doors to novel therapeutic avenues., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2023 Chinnaperumal Kamaraj et al.)

Cucurbits are an essential summer-season vegetable crops, but they are highly vulnerable from a range of abiotic and biotic factors. One of the significant biotic factors posing a growing menace to the production of major cucurbits in India is the emergence of tomato-infecting begomoviruses. In this study, we utilized PCR-based species-specific primers, developed earlier in our laboratory for the detection of begomoviruses infecting tomato and chilli plants, to identify begomoviruses in cucurbits across various regions of India. Leaf samples from major cucurbits were collected from different regions of Haryana, Delhi, Uttar Pradesh, Chhattisgarh, Maharashtra, Telangana and Karnataka, during the year 2020-2021. Total nucleic acid (TNA) was extracted from the samples and subjected to PCR using a generic primer specific to begomoviruses. The samples that exhibited positive amplification were further tested using six different species-specific primers targeting specific begomovirus species, namely Tomato leaf curl New Delhi virus (ToLCNDV), Tomato leaf curl Palampur virus (ToLCPalV), Tomato leaf curl Bangalore virus (ToLCBV), Tomato leaf curl Joydebpur virus (ToLCJoV), Tomato leaf curl Gujarat virus (ToLCGuV), and Chilli leaf curl virus (ChiLCV). The PCR analysis revealed that among the 551 plant samples tested, a total of 124 samples exhibited positive amplification using the universal begomovirus PCR. Specifically, 47 samples tested positive for ToLCNDV, 73 samples were positive for ToLCPalV and only one sample showed positive amplification for ChiLCV. However, none of the samples tested positive for ToLCJoV, ToLCGuV and ToLCBV. These findings from our study indicate the prevalence of ToLCNDV and ToLCPalV in major cucurbits across India. Furthermore, the study highlights the varied distribution of begomoviruses in major cucurbits between northern and southern regions of India., Competing Interests: Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. We are declaring that to the best of our knowledge, there are no conflict of interest exists in the content of the manuscript., (© The Author(s), under exclusive licence to Indian Virological Society 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

Introduction: Empowering women in a tribal context through entrepreneurship is an approach for enabling and making them economically and socially viable. This paper aims to highlight entrepreneurship in a specific tribal context and provide insight on some instances or cases relating to women's empowerment. Although there are many initiatives from international organizations and governmental institutions to support women entrepreneurs, especially tribal women, they suffer from isolation as a result of their language and lifestyle differ from the rest of society, which made the growth of their business and ability to compete arduous, and thus affected their ability to make various decisions in their lives. This study examines the pathway to a better understanding of increasing access to entrepreneurship for tribal women in Mayurbhanj district, Odisha., Methods: The sample size was 111 Santhal tribal women entrepreneurs, and all of them were interviewed using an interview schedule and Focus Group Discussions (FGDs). Two analytical tools were used (a linear regression model to find which dependent variables influence entrepreneurs and the Women's Empowerment Index (WEI) to measure the progress in social and economic opportunities). The respondents was interviewed and asked on the various WEI indicators before and after an entrepreneurship intervention., Results and Discussion: It was observed through the results that the empowerment of women has changed in a positive direction after establishing their work; according to the indicators of the study, the Women's Empowerment Index has changed from 0.61 to 1.26. It was also found that entrepreneurship has a positive and significant impact on women's decision-making within the family, and therefore, it was suggested through research to increase the intervention from the government and related organizations with more initiatives that contribute to the possibility of increasing women's education and their financial ability to open new enterprises., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Naveen, Parida and Panda.)

Introduction: Recent developments characterizing the pathophysiological basis of infection in the Coronavirus disease - 19 (COVID-19) have stirred great interest in studying this disease outside the purview of respiratory involvement and especially focusing on the gastrointestinal (GI) system. The present study involving a large cohort of COVID-19-infected patients reports on the characteristics of GI manifestations in patients infected with COVID-19 as well as the predictive role in their association with disease severity and adverse outcomes., Methods: A retrospective cohort study was carried out in a tertiary care hospital in northern India. Descriptive analysis of GI symptoms was carried out followed by predictive analysis assessing COVID-19 severity and with the primary endpoint of 28-day in-hospital all-cause mortality., Results: Of 3842 hospitalized COVID-19 patients, 2113 (55%) were symptomatic. GI symptoms were present in 163 (7.1%) patients. Common GI symptoms were diarrhea 65 (3.1%), anorexia 61 (2.9%) and vomiting 37 (1.8%). Mild and moderate-to-severe disease was seen in 1725 (81.6%) and 388 (18.4%) patients, respectively. Logistic regression showed greater odds of moderate-to-severe disease in patients with any GI symptoms (odds ratio [OR] 1.849, 95% CI 1.289-2.651 [p = 0.001]) and anorexia in particular (OR 2.797, 95% CI 1.647-4.753 [p = 0.001]); however, on multivariable-analysis, this association lost its significance. A total of 172 patients succumbed to illness. In the Cox proportional hazards model for mortality, patients with any GI symptom (HR 2.184, 95 CI 1.439-3.317 [p < 0.001]) and anorexia (HR 3.556, 95% CI 2.155-5.870 [p < 0.001]) had higher risk. In multi-variable analysis after adjustment to age, sex, oxygen saturation and comorbidities, the presence of any GI symptom was a significant predictor of mortality (hazard ratio adjusted [HR adj ] 1.758, 95% CI 1.147-2.694 [p = 0.010])., Conclusion: GI symptoms were common among patients infected with COVID-19. The presence of any GI symptom was a significant predictor of the risk of mortality after adjustment to respiratory failure, age, sex and pre-existing comorbidities. The clinical and pathophysiological basis of these associations has been explored., (© 2023. Indian Society of Gastroenterology.)

Cucumber green mottle mosaic virus (CGMMV, genus Tobamovirus ) is a widely occurring tobamovirus in cucurbits. The genome of CGMMV has been used previously for the expression of foreign genes in the plant. High throughput delivery and high viral titer are important requirements of foreign protein expression in plant through virus genome-based vector, in this study, Agrobacterium containing infectious construct of CGMMV was infiltrated through syringe, vacuum and high-speed spray to N. benthamiana, cucumber and bottle gourd leaves. The success rate of systemic infection of CGMMV agro-construct through all three methods was higher (80-100%) in N. benthamiana compared to the cucurbits (40-73.3%). To determine the high-throughput delivery of CGMMV in the plant system, four delivery methods viz. rubbing, syringe infiltration, vacuum infiltration and high-speed spray using the progeny virus derived through CGMMV agro-construct were compared in the three different plant species. Based on the rate of systemic infection and time required to perform delivery by different methods, vacuum infiltration was found most efficient for the high-throughput delivery of CGMMV. The quantification of CGMMV through qPCR revealed that CGMMV load varied considerably in leaf and fruit tissues depending with the time of infection. Immediately after expression of symptoms, a high load of CGMMV (~ 1 µg/100 mg of tissues) was noticed in young leaves of N. benthamiana and cucumber. In bottle gourd leaves, the CGMMV load was far low compared to N. benthamiana and cucumber plants. In the fruit tissues of cucumber and bottle gourd higher virus load was observed in mature fruit but not in immature fruit. The findings of the present study will serve as an important base line information to produce foreign protein through CGMMV genome-vector., Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03630-y., Competing Interests: Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. We are declaring that to the best of our knowledge, there are no conflict of interest exists in the content of the manuscript., (© King Abdulaziz City for Science and Technology 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

The propensity of manufacturers to produce goods at affordable cost, with more accuracy, and at a faster rate force them to search for novel solutions, such as deploying robots in place of people in a sector that can accommodate their needs. Welding is one of the most crucial processes in the automotive industry. This process is time-consuming, subject to error, and demands skilled professionals. The robotic application can improve this area of production and quality. Other industries, such as painting and material handling, can also profit from the use of robots. This work describes the fuzzy DC linear servo controller, which functions as a robotic arm actuator. Robots have been widely employed in most productive sectors in recent years, including assembly plates, welding, tasks at higher temperatures, etc. Controlling a robot accurately is a difficult undertaking as a robot is very nonlinear with many joints that are often organized and unstructured. To carry out the effective task, an effective PID control based on fuzzy logic has been employed together with the method of Particle Swarm Optimization (PSO) approach for the estimate of the parameter. This offline technique determines the lowest number of optimal robotic arm control parameters. To verify the controller design with computer simulation, a comparative assessment of controllers is given by means of a fuzzy surveillance controller with PSO which improves the parameter gain to provide a rapid climb, a smaller overflow, no steady condition error signal, and effective torque control of the robot arm., (© 2023. The Author(s).)

Background: The inflammatory and immune factors play a key role in the pathogenesis of vitiligo, and there are very few studies that have investigated the levels of major cytokines produced by T helper (Th) 1, Th2, and Th17 cells. This can enable better understanding of the, pathogenesis, and severity of vitiligo., Objectives: To evaluate the serum levels of interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-1β, interferon (IFN)-γ, and IL-10 in patients with vitiligo and to correlate them with the disease severity and activity and to compare them with normal healthy controls., Materials and Methods: A case-control study was conducted with 100 study participants: 50 cases clinically diagnosed as vitiligo and 50 controls. All patients underwent complete evaluation with detailed demographic parameters, history, and physical examination. The severity of the disease was assessed clinically by Vitiligo Area Scoring Index (VASI) and Vitiligo Disease Activity Score (VIDA). Blood investigations performed were IL-6, TNF-α, IL-1β, IFN-γ, and IL-10., Results: We observed significantly higher levels of serum IFN-γ levels in the patient group when compared with those of the normal controls ( P = 0.002) and showed a positive correlation with the activity and severity of the disease with a significant VASI ( P = 0.05) and VIDA score ( P = < 0.001). The mean serum IL-10 (p < 0.001) in patients with vitiligo was significantly lower than that in the control group. There was no significant difference in the serum level of TNF-α level ( P = 0.347), IL-6 ( P = 0.365), and IL-1β ( P = 0.362) between vitiligo and healthy controls., Conclusion: This study proved that high serum level of IFN-γ may be a risk factor for vitiligo progression and significantly low levels of IL-10, which has an anti-inflammatory role, suggesting that they could be used as a marker for assessing vitiligo activity and may open the way for further therapeutic approaches for vitiligo., Competing Interests: There are no conflicts of interest., (Copyright: © 2023 Indian Dermatology Online Journal.)

Background: Trophic ulcers remain the most common reason for hospitalization in patients with Hansen's disease. With the introduction of new therapeutic regimens, leprosy can now be cured. However, complications of the disease, such as sensory loss, muscle palsy, absorption of extremities, and recurrent ulcers, still lead to substantial morbidity. The management of patients with trophic ulcers and their consequences is difficult, because it is a recurrent and recalcitrant problem., Aims: To evaluate the efficacy of autologous smashed follicular dermal graft and epidermal cell suspension (ECS) in the treatment of chronic nonhealing trophic ulcers in patients with Hansen's disease and to compare its efficacy with normal saline dressing., Materials and Methods: A total of 46 chronic nonhealing trophic ulcers were randomized into two groups (23 ulcers in each): Ulcers in Group A were treated with autologous smashed follicular dermal graft and ECS; ulcers in Group B were treated with normal saline dressings. Ulcers were assessed based on the rate of ulcer size reduction at every week till 12 weeks and then once a month till the sixth month., Results: All 23 (100%) ulcers in Group A had healed within the study period of six months, whereas only 14 (60.9%) ulcers had healed in Group B. Nine (39.1%) ulcers in Group B had not healed even at the end of six months. All 23 (100%) ulcers in Group A had healed within eight weeks, which was statistically significant, P value <0.05., Conclusion: Trophic ulcers heal faster by autologous smashed follicular dermal graft and ECS, with good results of re-epithelialization of the ulcer bed than by normal saline dressing., Competing Interests: There are no conflicts of interest., (Copyright: © 2023 Journal of Cutaneous and Aesthetic Surgery.)

The ability to detect glucose concentrations in human urine offers a non-invasive approach to monitor changes in blood glucose, kidney health and vascular complications associated with diabetes. We show the potential of employing catalytically active nanoparticles directly grown on textiles to produce a dose-dependent colorimetric sensor for glucose. We use a galvanic replacement (GR) reaction for the synthesis of bimetallic nanoparticles. Here, Cu nanoparticles act as a sacrificial template that undergoes a spontaneous electroless GR reaction when exposed to metal ions of gold, silver, platinum, and palladium to form bimetallic Cu-M nanoparticles (M = Au, Ag, Pt, or Pd). The evaluation of their intrinsic peroxidase-mimicking catalytic activity ("nanozyme") in comparison to that of the Cu nanozyme revealed that the bimetallic systems show a higher catalytic rate with the Cu-Pt nanozyme showing the highest catalytic efficiency. This property of the Cu-Pt nanozyme was then utilized to detect glucose in human urine using the glucose oxidase enzyme as a molecular recognition element. A key outcome of our study is the ability to detect urine glucose without requiring sample dilution which is an advantage over the gold standard GOx-POx method and significantly more reliable performance over commercial urine glucose dipsticks. The difference in the intensity of the colorimetric response between different glucose concentrations further allowed this sensor system to be combined with digital imaging tools for multivariate analysis., (Copyright © 2022 Elsevier B.V. All rights reserved.)

The hallmark of the Alzheimer's disease (AD) is the accumulation of aggregated, misfolded proteins. The cause for this accumulation is increased production of misfolded proteins and impaired clearance of them. Amyloid aggregation and tau hyperphosphorylation are the two proteinopathies which accomplish deprivation of cell and tissue hemostasis during neuropathological process of the AD, as a result of which progressive neuronal degeneration and the loss of cognitive functions. p38 mitogen-activated protein kinase (p38 MAPK) has been implicated in both the events associated with AD: tau protein phosphorylation and inflammation. p38α MAPK pathway is activated by a dual phosphorylation at Thr180 and Tyr182 residues. Clinical and preclinical evidence implicates the stress related kinase p38α MAPK as a potential neurotherapeutic target. Drug design of p38α MAPK inhibitors is mainly focused on small molecules that compete for Adenosine triphosphate in the catalytic site. Here we have carried out the synthesis of phenyl sulfonamide derivatives Sulfo (I) and Sulfo (II). Crystal structures of Sulfo (I) and Sulfo (II) were solved by direct methods using SHELXS-97 . Sulfo (I) and Sulfo (II) have R int values of 0.0283 and 0.0660, respectively, indicating good quality of crystals and investigated their ability against p38α MAPK. Docking studies revealed that the Sulfo (I) had better binding affinity (-62.24 kcal/mol) as compared to Sulfo (II) and cocrystal having binding affinity of -54.61 kcal/mol and -59.84 kcal/mol, respectively. Molecular dynamics simulation studies of Sulfo (I) and cocrystal of p38α MAPK suggest that during the course of 30 ns simulation run, compound Sulfo (I) attained stability, substantiating the consistency of its binding to p38α MAPK compared to cocrystal. Binding free energy analysis suggests that the compound Sulfo (I) is better than the cocrystal. Thus, this study corroborates the therapeutic potential of synthesized Sulfo (I) in combatting AD.Communicated by Ramaswamy H. Sarma.

Machining activities in recent times have shifted their focus towards tool life and tool wear. Cutting tools have been utilized on a daily basis and play a vital role in manufacturing industries. Prolonged and incessant operation of the cutting tool can lead to wear and tear of the component, thereby compromising the dimensional accuracy. The condition of a tool is estimated based upon the surface quality of the machined component, condition of the machine, and the rate of production. Maintaining the tool health plays a vital role in enhancing the productivity of manufacturing industries. Numerous efforts were experimented by the researchers to maintain the tool health condition. The drawbacks of conventional diagnostic techniques include requirement of high level of human intelligence and professional expertise on the field, which led the researchers to develop intelligent and automatic diagnostic tools. There are many techniques suggested by researchers to detect the condition of single point cutting tool. This article proposes the use of transfer learning technology to detect the condition of single point cutting tool. First, the vibration signals were collected from the cutting tool and plots were made which will work as input to the deep learning algorithms. The deep learning algorithms have the capability to learn from the plots of vibration signals and classify the state of the single point cutting tool. In this work, the pretrained networks such as VGG-16, AlexNet, ResNet-50, and GoogLeNet were employed to identify the state of the cutting tool. In the pretrained networks, the effect of hyperparameters such as batch size, solver, learning rate, and train-test split ratio was studied, and the best performing network was suggested for tool condition monitoring., Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this article., (Copyright © 2022 S. Naveen Venkatesh et al.)

Misfire detection in an internal combustion engine is an important activity. Any undetected misfire can lead to loss of fuel and power in the automobile. As the fuel cost is more, one cannot afford to waste money because of the misfire. Even if one is ready to spend more money on fuel, the power of the engine comes down; thereby, the vehicle performance falls drastically because of the misfire in IC engines. Hence, researchers paid a lot of attention to detect the misfire in IC engines and rectify it. Drawbacks of conventional diagnostic techniques include the requirement of high level of human intelligence and professional expertise in the field, which made the researchers look for intelligent and automatic diagnostic tools. There are many techniques suggested by researchers to detect the misfire in IC engines. This paper proposes the use of transfer learning technology to detect the misfire in the IC engine. First, the vibration signals were collected from the engine head and plots are made which will work as input to the deep learning algorithms. The deep learning algorithms have the capability to learn from the plots of vibration signals and classify the state of the misfire in the IC engines. In the present work, the pretrained networks such as AlexNet, VGG-16, GoogLeNet, and ResNet-50 are employed to identify the misfire state of the engine. In the pretrained networks, the effect of hyperparameters such as back size, solver, learning rate, and train-test split ratio was studied and the best performing network was suggested for misfire detection., Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this paper., (Copyright © 2022 S. Naveen Venkatesh et al.)

A local pedicled vascularized bone flap can prevent the morbidity and cost of free bone flap surgery in small segmental bone defects or long cartilaginous defects of the head and neck. Such flaps can also be useful in patients who are high risk for surgery. The periosteal vascularity of the mandible can be used to design islanded facial artery-based bone flaps, which can be utilized to that extent. Two patients with a small segmental mandibulectomy defect and one patient with a long cricotracheal resection defect underwent reconstruction using three different designs of islanded facial artery osteomyomucosal/osseous flap (iFOMM). The patients had a minimum follow-up period of 18 months. All flaps were successful, with satisfactory healing and without any functional deficit or disease at last follow-up., (Copyright © 2020 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.)

Renal complications are long-term effect of diabetes mellitus where glucose is excreted in urine. Therefore, reliable glucose detection in urine is critical. While commercial urine strips offer a simple way to detect urine sugar, poor sensitivity and low reliability limit their use. A hybrid glucose oxidase (GOx)/horseradish peroxidase (HRP) assay remains the gold standard for pathological detection of glucose. A key restriction is poor stability of HRP and its suicidal inactivation by hydrogen peroxide, a key intermediate of the GOx-driven reaction. An alternative is to replace HRP with a robust inorganic enzyme-mimic or NanoZyme. While colloidal NanoZymes show promise in glucose sensing, they detect low concentrations of glucose, while urine has high (mM) glucose concentration. In this study, a free-standing copper NanoZyme is used for the colorimetric detection of glucose in human urine. The sensor could operate in a biologically relevant dynamic linear range of 0.5-15 mM, while showing minimal sample matrix effect such that glucose could be detected in urine without significant sample processing or dilution. This ability could be attributed to the Cu NanoZyme that for the first time showed an ability to promote the oxidation of a TMB substrate to its double oxidation diimine product rather than the charge-transfer complex product commonly observed. Additionally, the sensor could operate at a single pH without the need to use different pH conditions as used during the gold standard assay. These outcomes outline the high robustness of the NanoZyme sensing system for direct detection of glucose in human urine. Graphical abstract.

Background: The process of odontogenesis is complex involving epithelial-mesenchymal interactions, along with the molecular signaling pathways triggering the initiating process. The triggering factors and cells precisely involved in the pathogenesis of odontogenic cysts and tumors are unknown. There is a vast array of biomarkers used to stain different sites, thereby helpful in diagnosing and evaluating the prognosis of these cysts and tumors. Cytokeratins are the intermediate filament proteins which maintain cell integrity and alter their properties in cysts and tumors. In the following study, cytokeratin 19 expression patterns are assessed quantitatively in reduced enamel epithelium, dentigerous cyst and unicystic ameloblastoma., Aim: The aim of present study is to assess expression of CK 19, a stem cell marker in reduced enamel epithelium, dentigerous cyst and unicystic ameloblastoma, quantitatively., Materials and Methods: The present study is carried out with 15 samples in each group. Reduced enamel epithelium is derived from the patients undergoing treatment for impacted teeth. Histopathologically diagnosed cases of dentigerous cyst and unicystic ameloblastoma were considered for the study. With the help of Olympus BX 43 microscope, with ProgRes microscope camera, the 45 slides obtained were examined. The region of interest was selected in each slide and number of cells positively stained was counted. Data were analyzed using SPSS software version 23. Descriptive for scale data, One way anova with post hoc Tukey's test for intergroup comparison., Results: The results showed significant P value <0.05. Expression of CK 19 was highest in reduced enamel epithelium, followed by dentigerous cyst and unicystic ameloblastoma., Conclusion: CK 19 can be used as diagnostic marker to differentiate between odontogenic cyst and tumor., Competing Interests: There are no conflicts of interest., (Copyright: © 2021 Journal of Oral and Maxillofacial Pathology.)

White tail disease (WTD) of cultured Macrobrachium rosenbergii  is caused by Macrobrachium rosenbergii nodavirus ( Mr NV) and extra small virus (XSV). Since both the viruses have small single strand RNA as genetic material with short generation time, they are more prone to mutations. Hence detection methods developed for one strain may be suboptimal for the detection of isolates from the different geographical locations. In the present study two new genomic based methods (RT-PCR and dot-blot hybridization) along with one immunological method (polyclonal antibodies based detection) were developed for the detection of Indian isolates of Mr NV and XSV. Among genomic based methods, RT-PCR assay developed was most sensitive. Sensitivity of detection of RT-PCR was 1 fg (both Mr NV and XSV) of total RNA extracted from purified viral inoculum preparation. In case of WTD positive whole tissue total RNA, the limit of detection was 10 fg for both Mr NV and XSV. Dot-blot hybridization had a detection limit of 10 pg and 0.1 ng for Mr NV and XSV respectively when RNA extracted from viral inoculum preparation was used; 0.1 ng and 1 ng when WTD positive whole tissue total RNA was used. Polyclonal antibodies against recombinant proteins ( Mr NV and XSV capsid) were synthesised. Western blotting and indirect ELISA revealed that the antibodies produced to be specific and highly sensitive. Recombinant protein (antigen) of Mr NV and XSV capsid were detected at the dilution of 1:8000. However in case of infected prawn tissue sample, Mr NV and XSV were detected at the dilution of 1:32,000 and 1:64,000 respectively. All methods developed are field applicable., Competing Interests: Conflict of interestThere is no conflict of interest., (© Indian Virological Society 2020.)

Background: Simultaneous saccharification and fermentation (SSF) of pre-treated lignocellulosics to biofuels and other platform chemicals has long been a promising alternative to separate hydrolysis and fermentation processes. However, the disparity between the optimum conditions (temperature, pH) for fermentation and enzyme hydrolysis leads to execution of the SSF process at sub-optimal conditions, which can affect the rate of hydrolysis and cellulose conversion. The fermentation conditions could be synchronized with hydrolysis optima by carrying out the SSF at a higher temperature, but this would require a thermo-tolerant organism. Economically viable production of platform chemicals from lignocellulosic biomass (LCB) has long been stymied because of the significantly higher cost of hydrolytic enzymes. The major objective of this work is to develop an SSF strategy for D-lactic acid (D-LA) production by a thermo-tolerant organism, in which the enzyme loading could significantly be reduced without compromising on the overall conversion., Results: A thermo-tolerant strain of Lactobacillus bulgaricus was developed by adaptive laboratory evolution (ALE) which enabled the SSF to be performed at 45 °C with reduced enzyme usage. Despite the reduction of enzyme loading from 15 Filter Paper Unit/g LCB (FPU/g LCB ) to 5 FPU/g LCB , we could still achieve ~ 8% higher cellulose to D-LA conversion in batch SSF, in comparison to the conversion by separate enzymatic hydrolysis and fermentation processes at 45 °C and pH 5.5. Extending the batch SSF to SSF with pulse-feeding of 5% pre-treated biomass and 5 FPU/g LCB , at 12-h intervals (36th-96th h), resulted in a titer of 108 g/L D-LA and 60% conversion of cellulose to D-LA. This is one among the highest reported D-LA titers achieved from LCB., Conclusions: We have demonstrated that the SSF strategy, in conjunction with evolutionary engineering, could drastically reduce enzyme requirement and be the way forward for economical production of platform chemicals from lignocellulosics. We have shown that fed-batch SSF processes, designed with multiple pulse-feedings of the pre-treated biomass and enzyme, can be an effective way of enhancing the product concentrations., Competing Interests: Competing interestsThe authors declare that they have no competing interests., (© The Author(s) 2020.)

Competing Interests: There are no conflicts of interest.

The aim of this study was to determine whether the islanded facial artery myomucosal flap (iFAMM) is a good alternative to fasciocutaneous free flaps (FCFF) in the reconstruction of lateral oral tongue defects. This was a retrospective study of 40 patients with oral tongue cancers (lateral lesions not >4 cm) operated on between August 2014 and March 2017, who underwent primary reconstruction with either an iFAMM or FCFF. The two groups were compared with respect to intraoperative time, total intensive care unit (ICU) and hospital stay, complications, speech, swallowing, aesthetics, donor site morbidity, and economic feasibility. Patients who had an iFAMM had a reduced operating time, duration of ICU stay, and length of hospitalization; this flap was also more economically feasible. Better aesthetics and less donor site morbidity were also seen. The iFAMM is an alternative to FCFF in the reconstruction of lateral oral tongue defects, as it is less technically demanding, has good aesthetic outcomes, and is more economical, with acceptable donor site morbidity., (Copyright © 2020 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.)

Competing Interests: Competing interests: None declared.

6-Thioguanine encapsulated chitosan nanoparticles (6-TG-CNPs) has formulated by the ionic-gelation method. Morphologically, the 6-TG-CNPs were spherical and showed mean size, PDI, zeta potential, and entrapment efficiency of 261.63 ± 6.01 nm, 0.34 ± 0.10, +15.97 ± 0.46 mV and 44.27%, respectively. The IR spectra confirmed the 6-TG complex with chitosan. The in vitro drug release profile of 6-TG-CNPs revealed an increase in sustained-release (91.40 ± 1.08% at 48 h) at pH 4.8 compared to less sustained-release (73.96 ± 1.12% at 48 h) at pH 7.4. The MTT assay was conducted on MCF-7 and PA-1 cell lines at 48 h incubation to determine % cell viability. The IC 50 values of 6-TG, 6-TG-CNPs, and curcumin for MCF-7 were 23.09, 17.82, and 15.73 μM, respectively. Likewise, IC 50 values of 6-TG, 6-TG-CNPs, and curcumin for PA-1 were 5.81, 3.92, and 12.89 μM, respectively. A combination of 6-TG-CNPs (IC 25 ) with curcumin (IC 25 ) on PA-1 and MCF-7 showed % cell viability of 43.67 ± 0.02 and 49.77 ± 0.05, respectively. The in vitro cytotoxicity potential in terms of % cell viability, early apoptosis, G2/M phase arrest, and DNA demethylating activity of 6-TG-CNPs alone and combination with curcumin proved to be more effective than that of 6-TG on PA-1 cells., Competing Interests: Declaration of competing interest The authors declare that they have no competing interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Curcumin, a major bioactive component of turmeric (Curcuma longa), is known for its multiple health benefits. Curcumin as such is a mixture of its analogs: bisdemethoxycurcumin (BDMC)-3%, and demethoxycurcumin (DMC)-17%. Although the effect of curcumin on platelets is documented, the effect of BDMC and DMC on platelets is less studied. Considering the indispensable role played by platelets in hemostasis, thrombosis, inflammation, and immunity, the present study evaluates the effect of curcumin, DMC and BDMC on platelet apoptosis. The components of curcumin were purified by silica-gel column chromatography. The purity and mass analysis of the purified curcuminoids was determined by RP-HPLC and LC-MS respectively. When analyzed for platelet apoptotic markers, only BDMC demonstrated increased incidence of platelet apoptotic markers including increase in intracellular Ca 2+ , decrease in ∆ψm, alteration in BCl-2 family proteins, the release of cytochrome c, caspase activation, and PS externalization via activation of ERK activation. ERK inhibitor PD98059 significantly alleviated BDMC induced decrease in ∆ψm, alteration in BCl-2, caspase-8 activation and PS externalization. Our results demonstrate that curcumin, DMC and BDMC differentially act on platelet in inducing apoptosis and the study highlights that the toxicity associated with curcumin therapy might be attributed to BDMC in the mammalian system., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The title compound, C 15 H 10 ClNO 5 , is relatively planar with the two aromatic rings being inclined to each other by 3.56 (11)°. The central -C(=O)-C-O-C(=O)- bridge is slightly twisted, with a C-C-O-C torsion angle of 164.95 (16)°. In the crystal, mol-ecules are linked by C-H⋯O and C-H⋯Cl hydrogen bonds, forming layers parallel to the (101) plane. The layers are linked by a further C-H⋯O hydrogen bond, forming a three-dimensional supra-molecular structure. There are a number of offset π-π inter-actions present between the layers [inter-centroid distances vary from 3.8264 (15) to 3.9775 (14) Å]. Hirshfeld surface analyses, the d norm surfaces, electrostatic potential and two-dimensional fingerprint plots were examined to verify the contributions of the different inter-molecular contacts within the supra-molecular structure. The shape-index surface shows that two sides of the mol-ecule are involved in the same contacts with neighbouring mol-ecules, and the curvedness plot shows flat surface patches that are characteristic of planar stacking., (© Sheshadri et al. 2019.)

2-(4-Nitro-phen-yl)-2-oxoethyl picolinate, C 14 H 10 N 2 O 5 , was synthesized under mild conditions. The chemical and mol-ecular structures were confirmed by single-crystal X-ray diffraction analysis. The mol-ecules are linked by inversion into centrosymmetric dimers via weak inter-molecular C-H⋯O inter-actions, forming R 2 2 (10) ring motifs, and further strengthened by weak π-π inter-actions. Hirshfeld surface analyses, the d norm surfaces, electrostatic potential and two-dimensional fingerprint (FP) plots were used to verify the contributions of the different inter-molecular inter-actions within the supra-molecular structure. The shape-index surface shows that two sides of the mol-ecules are involved with the same contacts in neighbouring mol-ecules and curvedness plots show flat surface patches that are characteristic of planar stacking., (© Sanjeeva Murthy et al. 2019.)

The title com-pound, C 15 H 11 NO 5 , is relatively planar, with the planes of the two aromatic rings being inclined to each other by 3.09 (5)°. In the crystal, mol-ecules are linked by a pair of C-H⋯O hydrogen bonds, forming inversion dimers, which enclose an R 2 2 (16) ring motif. The dimers are linked by a further pair of C-H⋯O hydrogen-bonds forming ribbons enclosing R 4 4 (26) ring motifs. The ribbons are linked by offset π-π inter-actions [centroid-centroid distances = 3.6754 (6)-3.7519 (6) Å] to form layers parallel to the ac plane. Through Hirshfeld surface analyses, the d norm surfaces, electrostatic potential and two-dimensional fingerprint (FP) plots were examined to verify the contributions of the different inter-molecular contacts within the supra-molecular structure. The shape-index surface shows that two sides of the mol-ecule are involved with the same contacts in neighbouring mol-ecules, and the curvedness plot shows flat surface patches that are characteristic of planar stacking., (© Sheshadri et al. 2019.)

Alcohols are the essential chemicals used in a variety of pharmaceutical and chemical industries. The extreme purity of alcohols in many of such industrial applications is essential. Though distillation is one of the methods used conventionally to purify alcohols, the method consumes more energy and requires carcinogenic entertainers, making the process environmentally toxic. Alternatively, efforts have been made to focus research efforts on alcohol dehydration by the pervaporation (PV) separation technique using polymeric membranes. The present review is focused on alcohol dehydration using PV separation technique, which is the most efficient and benign method of purifying alcohols that are required in fine chemicals synthesis and developing pharmaceutical formulations. This review will discuss about the latest developments in the area of PV technique used in alcohol dehydration using a variety of novel membranes., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Temporomandibular joint ankylosis is one of the most challenging airway disorders associated with varying anatomical abnormalities like adenotonsillar hypertrophy, craniofacial malformations, macroglossia, etc. This case highlights the intubation difficulties confronted during the airway management of a 10-year-old girl presenting lately with bilateral temporomandibular joint ankylosis, hypoplastic mandible, and adenoid hypertrophy. This patient was intubated successfully by using a suction catheter assembly to negotiate the endotracheal tube across the adenoid, and an unmatched-size flexible intubation fiberscope through a "separate insertion" technique and external laryngeal manipulation. This case emphasises the significance of a comprehensive preoperative evaluation in preparing the anaesthetic plan of an anticipated difficult airway in a paediatric population, having diverse anatomical hurdles presenting concurrently.

This study describes the muscle transcriptome profile of Bandur breed, a consumer favoured, meat type sheep of India. The transcriptome was compared to the less desirable, unregistered local sheep population, in order to understand the molecular factors related to muscle traits in Indian sheep breeds. Bandur sheep have tender muscles and higher backfat thickness than local sheep. The longissimus thoracis transcriptome profiles of Bandur and local sheep were obtained using RNA sequencing (RNA Seq). The animals were male, non-castrated, with uniform age and reared under similar environment, as well as management conditions. We could identify 568 significantly up-regulated and 538 significantly down-regulated genes in Bandur sheep (p≤0.05). Among these, 181 up-regulated and 142 down-regulated genes in Bandur sheep, with a fold change ≥1.5, were considered for further analysis. Significant Gene Ontology terms for the up-regulated dataset in Bandur sheep included transporter activity, substrate specific transmembrane, lipid and fatty acid binding. The down-regulated activities in Bandur sheep were mainly related to RNA degradation, regulation of ERK1 and ERK2 cascades and innate immune response. The MAPK signaling pathway, Adipocytokine signaling pathway and PPAR signaling pathway were enriched for Bandur sheep. The highly connected genes identified by network analysis were CNOT2, CNOT6, HSPB1, HSPA6, MAP3K14 and PPARD, which may be important regulators of energy metabolism, cellular stress and fatty acid metabolism in the skeletal muscles. These key genes affect the CCR4-NOT complex, PPAR and MAPK signaling pathways. The highly connected genes identified in this study, form interesting candidates for further research on muscle traits in Bandur sheep., Competing Interests: The authors have declared that no competing interests exist.

The title mol-ecule, C 11 H 8 F 3 NO 3 , adopts a cis configuration across the -C=C- double bond in the side chain and the dihedral angle between the phenyl ring and side chain is 47.35 (1)°. The -COOH group adopts a syn conformation (O=C-O-H = 0°), unlike the anti conformation observed in related maleamic acids. In the crystal, inversion dimers linked by pairs of O-H⋯O hydrogen bonds are connected via N-H⋯O hydrogen bonds and C-H⋯O inter-actions into (100) sheets, which are cross-linked by another C-H⋯O inter-action to result in a three-dimensional network. The Hirshfeld surface fingerprint plots show that the highest contribution to surface contacts arises from O⋯H/H⋯O contacts (26.5%) followed by H⋯F/F⋯H (23.4%) and H⋯H (17.3%).

Background: Similar to several other occupations, dentists are also stricken by bounteous occupational health hazards (OHHs) such as musculoskeletal disorders (MSDs) and ocular health-related issues., Aims: The aim of this study was to evaluate the diffusion of MSDs and ocular health-related issues among dental surgeons in Salem city, Tamil Nadu, India, according to age, sex, and number of practicing hours per week., Objectives: A survey was conducted to examine the rate of prevalence of these OHHs amidst the dental surgeons in Salem city., Materials and Methods: The study was performed through a questionnaire amidst 150 dentists practicing in Salem City, Tamil Nadu, India. Dental surgeons were questioned about any occupational disorder that they had experienced related to a musculoskeletal pain and its location whether in the back, upper limbs, or lower limbs. In extension to this, dental surgeons were asked about any ocular injury that they had., Results: MSD showed higher rate of occurrence correlated to ocular health-related issues., Conclusion: The practice of protective measures is crucial, in perspective of the high rate of these disorders in the community of dental surgeons., Competing Interests: There are no conflicts of interest.

Aims and Objectives: The aim of the study is to assess the effectiveness of lag screw fixation in mandibular fractures with respect to parameters such as stability of the occlusion, functional stability, infection rates, neurosensory deficit, ease of technique, and maximum interincisal opening., Materials and Methods: Our study was carried out on 13 patients with mandibular fractures. Selected cases were treated with lag screw technique and 2.7 mm titanium screws were used. Postoperative complications such as wound dehiscence, infection, neurosensory disturbance, nonunion, malocclusion, postoperative mouth opening, and occlusal discrepancies were assessed., Results: All the patients were male, aged between 17 and 50 years. The cause of the injury was road traffic accidents in all, except for two assaults. Our study of 13 cases of fractured mandible included five symphyses, five parasymphyses, two angle, and one body fracture cases. Our observation with open reduction and rigid internal fixation with 1-2 lag screws revealed close approximation of fractured fragments in all the cases, except in three patients who had mild discrepancy of occlusion., Conclusion: Lag screw technique provides good interfragmentary compression and restoration of premorbid anatomic alignment of fracture fragments. In displaced mandibular fractures, especially in oblique fractures, the use of lag screw has proven to be ideal. The complications were found to be low. It was concluded that the lag screw provided excellent stability and occlusion., Competing Interests: There are no conflicts of interest.

The paper and pulp industry (PPI) produces high quantities of solid and liquid discharge and is regarded as the most polluting industry in the world causing adverse effects to environments and human beings. Hence changes in the way PPI sludge and waste materials are treated is urgently required. Nearly, 10 million tons of waste is generated per year, however PPI waste is enriched with many organic chemicalscontaining a high percentage of lignin, cellulose, and hemicellulose which can be used as valuable raw materials for the production of bioenergy and value-added chemicals. Pretreatment of complex lignocellulosic materials of PPI waste is difficult because of the cellulose crystallinity and lignin barrier. At present most of this waste is recycled in a conventional treatment approach through biological and chemical processes, incurring high cost and low returns. Henceefficient pretreatment techniques are required by which complete conversion of PPI waste is possible. Therefore, the present chapter provides the scope of integration of pretreatment methods through which bioenergy recovery is possible during the PPI waste treatment. Detailed information is presented on the various pre-treatment techniques (chemical, mechanical, enzymatic and biological) in order to increase the efficiency of PPI waste treatment and energy recovery from PPI waste. Along with acid and alkali based efficient chemical treatment process, physical methods (i.e. shearing, high-pressure homogenization, etc.), biochemical techniques (whole cell-based and enzyme-based) and finally biological techniques (e.g. aerobic and anaerobic treatment) are discussed. During each of the treatment processes, scope of energy recovery and bottlenecks of the processes were elaborated. The review thus provides systemic insight into developing efficient pretreatment processes which could increase carbon recovery and treatment efficiency of PPI waste., (Copyright © 2019. Published by Elsevier B.V.)

Prebiotics plays an important role in improving the growth of gut bacteria and it majorly found in various natural food sources such as fruits and vegetables. Nowadays, the prebiotic sources are added as a supplement in various food products such as dairy products, beverages, health drinks, infant formulae, and meat products. The presence of prebiotics provides various health benefits such as improveing calcium and magnesium absorption, increases bone density, reduces cancer risk, decreases cardiovascular diseases and also improves the immune system., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The present work explored biosorption of Zn(II) ions from aqueous and zinc-bearing factory effluent using marine seaweed Ulva lactuca. The batch pH edge experiments using aqueous zinc solution indicated that Zn(II) uptake by U. lactuca was found to be maximum at pH 4.5 and the batch isotherm trials performed at pH 4.5 resulted in maximum uptake capacity of 128.0 mg Zn(II)/g. With 0.1 M CaCl 2 (pH 3.5, HCl) as elutant, the elution of Zn(II) ions from Zn(II)-laden U. lactuca biosorbent was effective with possible regeneration and reuse for three cycles. The zinc industrial effluent was found to comprise of 87.8 mg/L of zinc ions along with excess co-ions and high total dissolved solids (838.1 mg/L). Owing to this, Zn(II) uptake from electroplating effluent by U. lactuca was suppressed due to competition from other ions. Continuous-flow sorption trials were conducted at flow rate of 5 mL/min in an up-flow fixed column. The existence of surplus competing ions in zinc wastewater influenced the Zn(II) biosorption by U. lactuca. U. lactuca-loaded packed column exhibited uptakes of 78.3 and 70.8 mg Zn(II)/g for aqueous solution and effluent, respectively. The results of three continuous sorption-desorption cycles demonstrated that reuse of U. lactuca biosorbent in remediation of zinc-containing wastewaters was practical and economical.

We have determined the protective effects of Thymus serpyllum (TS) extract and nanoparticle-loaded TS on hydrogen peroxide-induced cell death of mesenchymal stromal cells (MSCs) in vitro . Gas chromatography-mass spectroscopy confirmed the spectrum of active components in the extract. Out of the three different extracts, the hexane extract showed significant free radical scavenging activity. Treatment of MSCs with H 2 O 2 (hydrogen peroxide) significantly increased intracellular cell death; however, pretreatment with TS extract and nanoparticle-loaded TS (200  μ g/ml) suppressed H 2 O 2 -induced elevation of Cyt-c and MMP13 and increased the survival rates of MSCs. H 2 O 2 -induced (0.1 mM) changes in cytokines were attenuated in the extract and nanoparticles by pretreatment and cotreatment at two time points ( p < 0.05). H 2 O 2 increased cell apoptosis. In contrast, treatment with nanoparticle-loaded TS suppressed the percentage of apoptosis considerably ( p < 0.05). Therefore, TS may be considered as a potential candidate for enhancing the effectiveness of MSC transplantation in cell therapy.

Synthesis and isolation of highly unstable azirinobenzoxazole and benzoxazines in a chemodivergent fashion from aryl azido vinylogous carbonates by simple change in transition metal acetate is described. Thermal or rhodium(II) acetate-mediated decomposition of these azides gave dihydroazirino benzoxazole. Their nickel(II) acetate-promoted reaction gave 4-dihydro-2H-benzoxazines, whereas copper(II) acetate led to the corresponding oxidized imine derivatives. Benzaoxazine derivative could be kinetically resolved using a proline-catalyzed Mannich reaction. The benzoxazines were rapidly elaborated to angularly fused tetracyclic systems and coumarin-fused derivatives in a "one pot" fashion., (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

In the title chalcone derivative, C 15 H 9 BrCl 2 O, the aryl rings are inclined to each by 14.49 (17)°, and the configuration about the C=C bond is E . There is a short intra-molecular C-H⋯Cl contact present resulting in the formation of an S (6) ring motif. In the crystal, the shortest inter-molecular contacts are Cl⋯O contacts [3.173 (3) Å] that link the mol-ecules to form a 2 1 helix propagating along the b -axis direction. The helices stack up the short crystallographic a axis, and are linked by offset π-π inter-actions [inter-centroid distance = 3.983 (1) Å], forming layers lying parallel to the ab plane. A qu-anti-fication of the inter-molecular contacts in the crystal were estimated using Hirshfeld surface analysis and two-dimensional fingerprint plots.

In this article, a new reversed-phase high-performance liquid chromatography (RP-HPLC) method for the rapid, selective and sequential separation of toxic heavy metal ions namely, Pb 2+ , Hg 2+, and Cd 2+ is reported. For this RP-HPLC method, a C 18 silica monolith column (Chromolith RP-18e, 100 × 4.6 mm) has been surface modified using a laboratory-synthesized amphiphilic organic ligand namely, 1,5-dioctanoyl-1,5-diphenylcarbazide (DODPC), which acts an ion-selective receptor, for the separation of the target analytes. The eluted metal ions were subjected to post-column derivatization reaction with 4-(2-pyridylazo) resorcinol (PAR) prior to their detection using a UV-vis detector (at 520 nm). The post-column reagent (PAR; 4.7 × 10 -4 M; pH 9.0; 1.5 mL/min) was mixed with the column eluate through an ingeniously designed T-connector. An ultra-fast separation of Pb 2+ , Cd 2+, and Hg 2+ ions with a retention time of 1.67, 1.88 and 3.62 min, respectively was achieved, using 0.0526 mmol of DODPC coated C 18 monolithic column along with tartaric acid solution (1.0 × 10 -2 M; pH 4.0; 1.0 mL/min), as the isocratic eluent (mobile phase). The chromatographic parameters such as linearity, accuracy, recovery, limits of detection and quantification were validated to achieve superior analytical results. The influence of various analytical parameters such as nature of mobile phase and its concentration, solution pH, flow rate, post-column reagent and its concentration were studied and optimized. The studies revealed a lower detection limits of 0.075, 0.090 and 0.120 μg/L, and a quantification limits of 0.225, 0.270 and 0.450 μg/L, for Pb 2+ , Cd 2+ and Hg 2+ ions, respectively. A linear signal response in the concentration range of 0.05-50.0 μg/mL, was observed for the target metal ions, with an average r 2 value of 0.9994. The method was selective for the target metal ions, with excellent data reliability and reproducibility that accounts for an average recovery value of 99.76%, with a relative standard deviation of ≤1.83%., (Copyright © 2018 Elsevier B.V. All rights reserved.)

In the title chalcone-thio-phene derivative, C 13 H 6 Cl 3 FOS, the aromatic rings are inclined to one another by 12.9 (2)°, and the thio-phene ring is affected by π-conjugation. In the crystal, mol-ecules are linked by C-H⋯F hydrogen bonds, forming an R 2 2 (8) ring motif. A Hirshfeld surface analysis was conducted to verify the contribution of the different inter-molecular inter-actions. The shape-index surface clearly shows that the two sides of the mol-ecules are involved in the same contacts with neighbouring mol-ecules and the curvedness plots show flat surface patches characteristic of planar stacking.

2-Amino-pyridine and citric acid mixed in 1:1 and 3:1 ratios in ethanol yielded crystals of two 2-amino-pyridinium citrate salts, viz . C 5 H 7 N 2 + ·C 6 H 7 O 7 - ( I ) (systematic name: 2-amino-pyridin-1-ium 3-carb-oxy-2-carb-oxy-methyl-2-hy-droxy-propano-ate), and 3C 5 H 7 N 2 + ·C 6 H 5 O 7 3- ( II ) [systematic name: tris-(2-amino-pyridin-1-ium) 2-hy-droxy-propane-1,2,3-tri-carboxyl-ate]. The supra-molecular synthons present are analysed and their effect upon the crystal packing is presented in the context of crystal engineering. Salt I is formed by the protonation of the pyridine N atom and deprotonation of the central carb-oxy-lic group of citric acid, while in II all three carb-oxy-lic groups of the acid are deprotonated and the charges are compensated for by three 2-amino-pyridinium cations. In both structures, a complex supra-molecular three-dimensional architecture is formed. In I , the supra-molecular aggregation results from N amino -H⋯O acid , O acid ⋯H-O acid , O alcohol -H⋯O acid , N amino -H⋯O alcohol , N py -H⋯O alcohol and C ar -H⋯O acid inter-actions. The mol-ecular conformation of the citrate ion (CA 3- ) in II is stabilized by an intra-molecular O alcohol -H⋯O acid hydrogen bond that encloses an S (6) ring motif. The complex three-dimensional structure of II features N amino -H⋯O acid , N py -H⋯O acid and several C ar -H⋯O acid hydrogen bonds. In the crystal of I , the common charge-assisted 2-amino-pyridinium-carboxyl-ate heterosynthon exhibited in many 2-amino-pyridinium carboxyl-ates is not observed, instead chains of N-H⋯O hydrogen bonds and hetero O-H⋯O dimers are formed. In the crystal of II , the 2-amino-pyridinium-carboxyl-ate heterosynthon is sustained, while hetero O-H⋯O dimers are not observed. The crystal structures of both salts display a variety of hydrogen bonds as almost all of the hydrogen-bond donors and acceptors present are involved in hydrogen bonding.