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Among the plethora of affinity biosensor systems based on biomolecular recognition and labeling assays, magnetic labeling and detection is emerging as a promising new approach. Magnetic labels can be non-invasively detected by a wide range of methods, are physically and chemically stable, relatively inexpensive to produce, and can be easily made biocompatible. Here we provide an overview of the various approaches developed for magnetic labeling and detection as applied to biosensing. We illustrate the challenges to integrating one such approach into a complete sensing system with a more detailed discussion of the compact Bead Array Sensor System developed at the U.S. Naval Research Laboratory, the first system to use magnetic labels and microchip-based detection.

A significant challenge for all biosensor systems is to achieve high assay sensitivity and specificity while minimizing sample preparation requirements, operational complexity, and sample-to-answer time. We have achieved multiplexed, unamplified, femtomolar detection of both DNA and proteins in complex matrices (including whole blood, serum, plasma, and milk) in minutes using as few as two reagents by labeling conventional assay schemes with micrometer-scale magnetic beads, and applying fluidic force discrimination (FFD). In FFD assays, analytes captured onto a microarray surface are labeled with microbeads, and a controlled laminar flow is then used to apply microfluidic forces sufficient to preferentially remove only nonspecifically bound bead labels. The density of beads that remain bound is proportional to the analyte concentration and can be determined with either optical counting or magnetoelectronic detection of the magnetic labels. Combining FFD assays with chip-based magnetoelectronic detection enables a simple, potentially handheld, platform capable of both nucleic acid hybridization assays and immunoassays, including orthogonal detection and identification of bacterial and viral pathogens, and therefore suitable for a wide range of biosensing applications.

We have measured the efficiency over the range 125-225 A of a bare ion-etched plane laminar holographic grating made of fused silica and with 1000 grooves/mm. The measured efficiency of each order oscillates with wavelength because of constructive and destructive interference between radiation diffracted from the lands and the grooves. We measured the grating groove profile with an atomic force microscope, and the resulting groove depth of 434 ? 6 A agrees well with the values determined independently from the oscillatory behavior of the efficiency measurements. Grating efficiency in the +1 order peaked at values of 0.027%, 0.011%, and 0.005% at wavelengths of 191, 157, and 132 A, respectively; and the derived groove efficiencies are 27%, 25%, and 27%. The irregular shape at the land-groove edges dominates the large grating roughness of 23-45-A rms, but even regions far from the edges have a roughness of 10-18-A rms. The average groove profile was used to model the grating efficiency, and the resulting wavelengths predicted for different order maxima and minima agree well with measured wavelengths, although the calculated efficiencies are greater than the measured results by 10-20%.

We have fabricated the four flight gratings for a sounding rocket high-resolution spectrometer using a holographic ion-etching technique. The gratings are spherical (4000-mm radius of curvature), large (160 mm x 90 mm), and have a laminar groove profile of high density (3600 grooves/mm). They have been coated with a high-reflectance multilayer of Mo/Si. Using an atomic force microscope, we examined the surface characteristics of the first grating before and after multilayer coating. The average roughness is approximately 3 A rms after coating. Using synchrotron radiation, we completed an efficiency calibration map over the wavelength range 225-245 A. At an angle of incidence of 5 degrees and a wavelength of 234 A, the average efficiency in the first inside order is 10.4 +/- 0.5%, and the derived groove efficiency is 34.8 +/- 1.6%. These values exceed all previously published results for a high-density grating.

The normal-incidence efficiencies of two 4800-grooves/mm ruled replica gratings, one with a dual-bandpass molybdenum/silicon multilayer coating and the other with a gold coating, were measured by use of synchrotron radiation in the 125-325-A wavelength region. The peak reflectance of the multilayer coating was 22% in the first Bragg order near 235 A and 28% in the second Bragg order near 126 A. The peak efficiency of the multilayer grating was 2.6% in the first diffraction order near 225 A and 0.3% in the second diffraction order near 125 A. The efficiencies of the multilayer grating were much higher than the corresponding efficiencies of the gold grating. The characterization of the surfaces of the gratings by atomic force microscopy indicated rms microroughness values in the 5-18-microm(-1) frequency range of 12-20 A for the multilayer grating and 22-32 A for the gold grating. Both gratings had bumpy surface features larger than the nominal groove height. The rather large surface roughness and groove irregularities had a detrimental effect on the grating efficiencies.

Thin-film interference effects were observed in the normal-incidence efficiency of a 2400-groove/mm replica grating. The efficiency was measured in the 100-350-A wavelength range and had an oscillatory behavior that resulted from the presence of a thin SiO(2) coating. The thicknesses of the SiO(2) and the underlying oxidized aluminum layers were inferred from computer modeling of the zero-order efficiency. The efficiencies in the diffracted orders were calculated with the modified integral approach and accounting for the multilayer coating and the groove profile derived from atomic force microscopy. The calculated and measured efficiencies were in good agreement.

The efficiency of a diffraction grating was measured near normal incidence in the 125-225-A wavelength range with synchrotron radiation. The grating pattern had 2400 grooves/mm and was recorded on a concave fused-silica blank by a holographic technique. The grooves were shaped by ion-beam etching to provide a facet with a blaze angle of 2.5 degrees as determined by atomic force microscopy. Because of the characteristics of the etching process the groove profile was approximately triangular, with the other facet inclined at an angle of 5.5 degrees to the surface. The measured efficiency was compared with the efficiency calculated by a computer program, small enough to run on a personal computer, that solved the periodic boundary-value problem corresponding to electromagnetic radiation incident on a diffraction grating with finite conductivity. The calculation was based on the nominal groove profile that was determined by atomic force microscopy. The measured and the calculated efficiencies were in good agreement. This investigation indicates that the diffraction efficiency of a normal-incidence grating can be calculated in the soft-x-ray region with a personal computer.

We characterized a laminar grating with a Mo/Si multilayer coating by using synchrotron radiation and atomic force microscopy. The grating substrate had 2400 grooves/mm, 40-A groove depth, and 2080-A groove width. The microroughness of the grating substrate was 5 A rms. The multilayer coating was optimized to have peak normal-incidence reflectance at a wavelength near 150 A. For an angle of incidence of 10 degrees the peak grating efficiency was 16.3% in the +1 order and 15.0% in the -1 order. The efficiency in the zero order was lower by a factor of 40 owing to the excellent matching of the groove depth and groove width to the wavelength of the incident radiation. By dividing the grating efficiencies by the measured reflectance of the multilayer coating, we obtained inferred groove efficiencies of 34% and 32% in the +1 and -1 orders, respectively.

The efficiencies of replicas of the Skylab 3600-line/mm concave grating with multilayer and gold coatings were measured by using synchrotron radiation at an angle of incidence of 79 degrees and in the 28-42-A wavelength range. The blaze angle of the grating facets that faced the incident radiation was 3.1 degrees , and the average angle of the opposite facets was 6 degrees . For the gold grating, the -1 outside order had the highest efficiency of any diffracted order (excluding the zero order) over the entire wavelength range. Calculations of the grating efficiency indicated that the high efficiency in the -1 order resulted from the rather small angle (6 degrees ) of the facets opposite the incident radiation. For the multilayer grating, the efficiency in the on-blaze +2 inside order was enhanced in the 30-34-A wavelength region as a result of the high reflectance of the multilayer coating. The maximum efficiency in the +2 order occurred at the wavelength (32 A) corresponding to the peak of the reflectance of the multilayer coating on the facets facing the incident radiation. These results further demonstrate that a multilayer coating can be used to enhance the efficiency, in a selected wavelength range and in the on-blaze order, of a grating operating at a small grazing angle (11 degrees ).

The efficiency of an ion-etched laminar holographic grating was measured at near-normal incidence in the 14.5-16.0-nm wavelength range. The grating had an electron-beam-evaporated Mo/Si multilayer coating matched to the grating groove depth. The efficiency peaked at 16.3% in the first inside order at 15.12 nm and 15.0% in the first outside order at 14.94 nm. These are believed to be the highest efficiencies obtained to date from a multilayer-coated laminar grating at near-normal incidence in the EUV (lambda<30.0nm) . Zero and even orders were almost completely suppressed. The grating groove efficiency in the first order approached the theoretical limit of 40.5%.

Access to primary health care for indigent citizens presents a dilemma for many communities in the United States. In response, communities have developed a variety of strategies to effectively deal with the problem. This article describes the evolution of a small free clinic into a comprehensive primary care clinic developed through the actions of a community-based coalition. The clinic originated within an umbrella organization for indigent residents as free medical service provided at a night shelter by a local physician once a week. Through a coalition of business, religious, medical, hospital, foundation, lay volunteer, county health department, and chamber of commerce representatives, the service was enlarged into a formal clinic operation with a small staff and volunteers providing services for about 3,500 patient visits each year. As the demand for services increased beyond resources, an expanded coalition created HealthServe Medical Center, a comprehensive primary care clinic operating 40 hours per week. The HealthServe Board is currently active in supporting service delivery at the clinic, with plans to serve 24,000 medical and dental visits annually by mid-1995. The evolution process was based upon the characteristics of effective community coalitions and the commitment of individuals from diverse community sectors.

The efficiency of an ion-etched blazed holographic grating was measured by the use of synchrotron radiation in the 125-133-Å wavelength range and at near-normal incidence. The grating had a Mo-Si multilayer interference coating that resulted in a peak normal-incidence efficiency of 13% in the second grating order and at a wavelength of 128 Å. This is the highest efficiency obtained to date from a multilayer-coated grating in this wavelength region and at normal incidence. These measurements are compared with similar measurements performed on the same grating 4.5 years later. Over this time the peak grating efficiency decreased from 13% to 8%, and this result is attributed to the decrease in the reflectance of the multilayer coating from 55% to 42%. Oxidation and contamination of the multilayer with carbon appear to be the causes of these losses. The groove efficiency of the grating substrate in the second order is 23%.

A molybdenum/silicon multilayer coating was applied to a holographic ion-etched blazed grating substrate that had 2400 grooves/mm and a radius of curvature of 2.2 m. Scanning probe microscopy yielded the same surface microroughness (5 Å rms) before and after deposition of the multilayer. The efficiency and polarization performance of the grating was measured by synchrotron radiation in the 135-250-Å wavelength region. In the second grating order and the second Bragg order of the multilayer coating, the peak normal-incidence efficiency was 7.5% at a wavelength of 147 Å, representing a groove efficiency of 27%. At an angle of incidence of 35°, the polarization performance of the grating was 95%-100% in the 210-250-Å wavelength region. In a Seya-Namioka spectrometer mount at an angle of incidence of 30°-40°, the grating is a nearly perfect polarizing optical element in the wavelength bands between 125 and 300 Å, which are covered by the multilayer coating.

Multilayer and gold coatings were applied to replicas of the 3600-line/mm ruled grating that was developed for the Naval Research Laboratory S082A spectroheliograph that was flown on the Skylab spacecraft. The Mo-Si multilayer coating had a peak normal-incidence reflectance of 50% at a wavelength of 136 Å. The normal-incidence efficiency of the multilayer-coated grating was measured by the use of synchrotron radiation and was compared with the efficiency of the gold-coated replica grating in the 115-340-Å wavelength region. The peak efficiency of the multilayer grating was 1.3% in the 133-137-Å region and was a factor of 65 higher than the efficiency of the gold grating. The multilayer and gold coated gratings, as well as an uncoated replica grating substrate, were characterized by the use of a scanning probe microscope. The rms microroughness of the uncoated and multilayer-coated gratings was 10 Å, and the microroughness of the gold grating was 16 Å.

Although homelessness in urban areas has been examined extensively, little attention has been given to the problem of homelessness in rural areas. This article reports the findings from a 1990 statewide study of rural homelessness funded by the National Institute of Mental Health. During the six-month data collection period, 919 homeless adults were interviewed; 247 were heading family units, and 480 children were in these families. More than two-thirds of these families were headed by single parents. Implications of the findings for providing short-term emergency assistance and longer-term policy-making are presented.

The efficiency and resolving power of a concave, 2400-groove/mm, blazed diffraction grating that had a Mo/Si multilayer coating were determined. The multilayer coating had a peak reflectance of 55% at 140-A near normal incidence. The efficiency of the multilayer grating for wavelengths in the 136-139-A range was 2% near normal incidence. This efficiency was a factor of 150 greater than the efficiency of a sister replica Au-coated grating in the same wavelength region. The resolving power of the multilayercoated grating in the third order of a V viii transition with a first-order wavelength of 140.451 A was 9100. Comparisons with the Au-coated grating indicated that the application of the multilayer coating did not affect the resolving power or the blaze angle.

The normal-incidence reflectance of a Mo/Si multilayer mirror, with peak reflectance near 130 A, was measured over a period of 20 months by using synchrotron radiation. The measured reflectances were unchanged over this period of time, and this indicates that the material layers and interfaces were stable.

The measured normal-incidence reflectances of W/B(4)C multilayers mirrors are 2% in the 34-50-A wavelength region and are consistent with interfacial roughness in the range of 3-4 A.

A concave diffraction grating (2400 grooves/mm) coated with a Si/Mo multilayer has an efficiency of 2.5% at 290 A and a resolving power of 14,000 in third order of 156 A.

Using synchrotron radiation, we determined the optical constants of thin silicon films in the wavelength region near the silicon L(2,3) absorption edge. The extinction coefficient was determined from the transmittance of a thin, unbacked silicon film. The refractive index was determined from the reflectance of a sample consisting of an evaporated silicon film on gold. The thickness of the evaporated silicon film was chosen so that an interference feature appeared in the wavelength region above the edge where the silicon is transmissive. The shape of the interference feature is sensitive to the optical constants of silicon near the edge.

This article reports the findings of a National Institute of Mental Health (NIMH) services demonstration project that used a mobile case management team to serve homeless mentally ill clients. The project examined three issues: (1) factors associated with client engagement in case management, (2) clients' perceptions of how case management affected their quality of life, and (3) significant differences between clients who remained engaged in case management services and those who discontinued involvement. The results indicated that clients who received more frequent case management contact, had higher assessed independent living skills, were older, were less likely to be substance abusers, and had experienced fewer periods of homelessness and fewer prior psychiatric hospitalizations were more likely to remain engaged in case management services. After six months of case management, clients perceived significant improvement in their global well-being, living situation, use of leisure time, finances, and physical health. Implications for providing case management services to homeless mentally ill people are presented.

The research literature has documented the social and psychological consequences of unemployment for individuals in our country. Unfortunately, few studies have examined the impact of unemployment on the older worker. This exploratory study examined the characteristics and experiences of seventy-three older unemployed workers, age fifty and above, who were discouraged and had stopped searching for a full-time job. The findings portray the discouraged older worker as potentially at risk of both economic and psychological difficulties. Participants reported feeling mildly depressed about their current unemployment situation, socially isolated and embarrassed, and were experiencing low life satisfaction generally. They also tended to use a low number of social and employment-related community services. Younger participants in the sample under age sixty, those with higher levels of education, and those suffering longer periods of unemployment tended to report having the greatest adjustment difficulties. These results reflect the challenges faced by the discouraged older worker and the need for targeted employment and supportive services to aid this population group.