1. Comparative Morphological and Molecular Genetic Characteristics of Cell and Tissue Strains of Experimental Rat Glioma 10-17-2 (Astrid-17).
- Author
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Alekseeva AI, Kudelkina VV, Khalansky AS, Sentyabreva AV, Miroshnichenko EA, Gulyaev MV, Rakitina KA, and Kosyreva AM
- Subjects
- Animals, Rats, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Ki-67 Antigen metabolism, Ki-67 Antigen genetics, Male, Astrocytoma genetics, Astrocytoma pathology, Astrocytoma metabolism, Gene Expression Regulation, Neoplastic, Cell Line, Tumor, Brain pathology, Brain metabolism, Brain Neoplasms genetics, Brain Neoplasms pathology, Brain Neoplasms metabolism, Glioma genetics, Glioma pathology, Glioma metabolism, Glial Fibrillary Acidic Protein genetics, Glial Fibrillary Acidic Protein metabolism, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor A metabolism
- Abstract
In order to obtain models of gliomas of varying degrees of malignancy, we performed morphological and molecular genetic study of a tissue strain of glioma 10-17-2 (Astrid-17) obtained by intracranial passaging of tumor fragments of chemically induced rat brain tumor, and a cell strain isolated from it. More or less pronounced changes in the expression levels of Mki67, Trp53, Vegfa, and Gfap genes in the tissue and cell strain of glioma 10-17-2 (Astrid-17) compared with intact brain tissue were shown. The tissue model of glioma 10-17-2 (Astrid-17) according to the studied characteristics shows features of grade 3-4 astrocytoma and the cellular model - grade 2-3 astrocytoma., (© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2024
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