22 results on '"REYNALDI, Francisco"'
Search Results
2. First large-scale study reveals important losses of managed honey bee and stingless bee colonies in Latin America.
- Author
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Requier F, Leyton MS, Morales CL, Garibaldi LA, Giacobino A, Porrini MP, Rosso-Londoño JM, Velarde RA, Aignasse A, Aldea-Sánchez P, Allasino ML, Arredondo D, Audisio C, Cagnolo NB, Basualdo M, Branchiccela B, Calderón RA, Castelli L, Castilhos D, Escareño FC, Correa-Benítez A, da Silva FO, Garnica DS, de Groot G, Delgado-Cañedo A, Fernández-Marín H, Freitas BM, Galindo-Cardona A, Garcia N, Garrido PM, Giray T, Gonçalves LS, Landi L, Malusá Gonçalves D, Martinez SI, Moja PJ, Molineri A, Müller PF, Nogueira E, Pacini A, Palacio MA, Parra GN, Parra-H A, Peres Gramacho K, Castro EP, Pires CSS, Reynaldi FJ, Luis AR, Rossini C, Sánchez Armijos M, Santos E, Scannapieco A, Spina YM, Tapia González JM, Vargas Fernández AM, Viana BF, Vieli L, Yadró García CA, and Antúnez K
- Subjects
- Animals, Bees physiology, Latin America, Beekeeping, Seasons
- Abstract
Over the last quarter century, increasing honey bee colony losses motivated standardized large-scale surveys of managed honey bees (Apis mellifera), particularly in Europe and the United States. Here we present the first large-scale standardized survey of colony losses of managed honey bees and stingless bees across Latin America. Overall, 1736 beekeepers and 165 meliponiculturists participated in the 2-year survey (2016-2017 and 2017-2018). On average, 30.4% of honey bee colonies and 39.6% of stingless bee colonies were lost per year across the region. Summer losses were higher than winter losses in stingless bees (30.9% and 22.2%, respectively) but not in honey bees (18.8% and 20.6%, respectively). Colony loss increased with operation size during the summer in both honey bees and stingless bees and decreased with operation size during the winter in stingless bees. Furthermore, losses differed significantly between countries and across years for both beekeepers and meliponiculturists. Overall, winter losses of honey bee colonies in Latin America (20.6%) position this region between Europe (12.5%) and the United States (40.4%). These results highlight the magnitude of bee colony losses occurring in the region and suggest difficulties in maintaining overall colony health and economic survival for beekeepers and meliponiculturists., (© 2024. The Author(s).)
- Published
- 2024
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3. Bacterial diversity using metagenomics of 16s rDNA in water kefir, an innovative source of probiotics for bee nutrition.
- Author
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Rodríguez MA, Fernández LA, Díaz ML, Gallo CA, Corona M, Evans JD, and Reynaldi FJ
- Subjects
- Animals, Bees microbiology, DNA, Bacterial analysis, Biodiversity, DNA, Ribosomal genetics, Animal Feed microbiology, Probiotics, Kefir microbiology, RNA, Ribosomal, 16S genetics, Metagenomics methods, Bacteria isolation & purification, Bacteria genetics, Bacteria classification
- Abstract
Water kefir is a sparkling, slightly acidic fermented beverage made from sugar, water, and water kefir grains, which are a mixture of yeast and bacteria. These grains produce a variety of fermentation compounds such as lactic acid, acetaldehyde, acetoin, ethanol and carbon dioxide. In this study, a high-throughput sequencing technique was used to characterize the bacterial composition of the original water kefir from which potential probiotics were obtained. We studied the bacterial diversity of both water kefir grains and beverages. DNA was extracted from three replicate samples of both grains and beverages using the Powerlyzer Microbial Kit. The hypervariable V1-V2 region of the bacterial 16S ribosomal RNA gene was amplified to prepare six DNA libraries. Between 1.4M and 2.4M base-pairs were sequenced for the library. In total, 28721971 raw reads were obtained from all the samples. Estimated species richness was higher in kefir beverage samples compared to grain samples. Moreover, a higher level of microbial alpha diversity was observed in the beverage samples. Particularly, the predominant bacteria in beverages were Anaerocolumna and Ralstonia, while in grains Liquorilactobacillus dominated, with lower levels of Leuconostoc and Oenococcus. Although the bacterial diversity in kefir grains was low because only three genera were the most represented, all of them are LAB bacteria with the potential to serve as probiotics in the artificial feeding of bees., (Copyright © 2024 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
- Published
- 2024
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4. Probiotics and in-hive fermentation as a source of beneficial microbes to support the gut microbial health of honey bees.
- Author
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Rodríguez MA, Fernández LA, Daisley BA, Reynaldi FJ, Allen-Vercoe E, and Thompson GJ
- Subjects
- Animals, Anti-Bacterial Agents immunology, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Beekeeping methods, Bees drug effects, Bees immunology, Bees microbiology, Fermentation immunology, Gastrointestinal Microbiome immunology, Probiotics pharmacology, Probiotics therapeutic use
- Abstract
Managed populations of honey bees (Apis mellifera Linnaeus; Hymenoptera: Apidae) are regularly exposed to infectious diseases. Good hive management including the occasional application of antibiotics can help mitigate infectious outbreaks, but new beekeeping tools and techniques that bolster immunity and help control disease transmission are welcome. In this review, we focus on the applications of beneficial microbes for disease management as well as to support hive health and sustainability within the apicultural industry. We draw attention to the latest advances in probiotic approaches as well as the integration of fermented foods (such as water kefir) with disease-fighting properties that might ultimately be delivered to hives as an alternative or partial antidote to antibiotics. There is substantial evidence from in vitro laboratory studies that suggest beneficial microbes could be an effective method for improving disease resistance in honey bees. However, colony level evidence is lacking and there is urgent need for further validation via controlled field trials experimentally designed to test defined microbial compositions against specific diseases of interest., (© The Author(s) 2023. Published by Oxford University Press on behalf of Entomological Society of America.)
- Published
- 2023
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5. Microbiological and chemical characterization of water kefir: An innovative source of potential probiotics for bee nutrition.
- Author
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Rodríguez MA, Fernández LA, Díaz ML, Pérez M, Corona M, and Reynaldi FJ
- Subjects
- Bees, Animals, Water, Beverages microbiology, Bacteria, Saccharomyces cerevisiae, Fermentation, Kefir microbiology, Probiotics
- Abstract
We evaluated the microbial composition of water kefir grains and beverage over the course of one year to determine whether the number and type of microorganisms changed over the time. Bacteria and yeast colonies with different morphologies were isolated from water kefir and their antimicrobial activity was evaluated against Paenibacillus larvae and Ascosphaera apis. A chemical characterization of kefir was also carried out. Our results confirmed that bacteria and yeasts were more numerous in kefir grains compared with those in the beverage. The counts of microorganisms declined, although an important microbial community was still present in kefir after the long storage period. Eleven strains which inhibited bee pathogens were isolated from kefir. Genotypic results demonstrated that these isolates included Lentilactobacillus hilgardii, Lentilactobacillus buchneri and Saccharomyces cerevisiae. Thus, water kefir may be an innovative source of potential probiotic strains for bee nutrition in order to control honeybee diseases., (Copyright © 2022 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
- Published
- 2023
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6. Genetic diversification of an invasive honey bee ectoparasite across sympatric and allopatric host populations.
- Author
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Beaurepaire A, Arredondo D, Genchi-García ML, Castelli L, Reynaldi FJ, Antunez K, Invernizzi C, Mondet F, Le Conte Y, and Dalmon A
- Subjects
- Africa, Animals, Bees, Biodiversity, Sympatry, Acaricides, Varroidae genetics
- Abstract
Invasive parasites are major threats to biodiversity. The honey bee ectoparasite, Varroa destructor, has shifted host and spread almost globally several decades ago. This pest is generally considered to be the main global threat to Western honey bees, Apis mellifera, although the damages it causes are not equivalent in all its new host's populations. Due to the high virulence of this parasite and the viruses it vectors, beekeepers generally rely on acaricide treatments to keep their colonies alive. However, some populations of A. mellifera can survive without anthropogenic mite control, through the expression of diverse resistance and tolerance traits. Such surviving colonies are currently found throughout the globe, with the biggest populations being found in Sub-Saharan Africa and Latin America. Recently, genetic differences between mite populations infesting surviving and treated A. mellifera colonies in Europe were found, suggesting that adaptations of honey bees drive mite evolution. Yet, the prevalence of such co-evolutionary adaptations in other invasive populations of V. destructor remain unknown. Using the previous data from Europe and novel genetic data from V. destructor populations in South America and Africa, we here investigated whether mites display signs of adaptations to different host populations of diverse origins and undergoing differing management. Our results show that, contrary to the differences previously documented in Europe, mites infesting treated and untreated honey bee populations in Africa and South America are genetically similar. However, strong levels of genetic differentiation were found when comparing mites across continents, suggesting ongoing allopatric speciation despite a recent spread from genetically homogenous lineages. This study provides novel insights into the co-evolution of V. destructor and A. mellifera, and confirms that these species are ideal to investigate coevolution in newly established host-parasite systems., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2022
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7. Equine rhinosporidiosis in Buenos Aires, Argentina.
- Author
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Tizzano MA, Della Vedova R, Lopez RA, Amor V, Zubía C, Córdoba SB, and Reynaldi FJ
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- Animals, Argentina epidemiology, Cities, Horses, Rhinosporidium, South America, Rhinosporidiosis diagnosis, Rhinosporidiosis epidemiology, Rhinosporidiosis veterinary
- Abstract
Rhinosporidiosis is caused by Rhinosporidium seeberi, a parasitic organism of the family Rhinosporideacea family, class Micomycetozoa. The disease is endemic in India; however, some cases were reported in Europe, Africa, North America, and South America. The aim of the present study is to report three cases of rhinosporidiosis in wild horses in different cities of Buenos Aires province, Argentina. We confirm the presence of R. seeberi in the analyzed samples using histopathological and PCR sequencing techniques., (Copyright © 2021 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
- Published
- 2022
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8. Current status of resistance to ivermectin in Rhipicephalus sanguineus sensu stricto infesting dogs in three provinces in Argentina.
- Author
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Daniele MR, Dadé MM, Álvarez JD, Reynaldi FJ, Errecalde JO, and Rodríguez-Vivas RI
- Subjects
- Animals, Argentina, Dogs, Ivermectin pharmacology, Larva, Dog Diseases parasitology, Rhipicephalus sanguineus, Tick Infestations drug therapy, Tick Infestations epidemiology, Tick Infestations veterinary
- Abstract
Intensive use of macrocyclic lactones for parasite control exerts strong selective pressure for arthropods such as ticks to become resistant to them. Rhipicephalus sanguineus sensu stricto is a tick and disease vector of significant public health and veterinary importance worldwide. We assessed the toxicological response to the macrocyclic lactone ivermectin (IVM) in R. sanguineus s.s. infesting dogs in Argentina. Samples of nine tick populations were obtained by inspecting dogs at veterinary clinics, hospitals, or rural areas in the provinces of San Luis, Rio Negro, and Buenos Aires. Pet owners were interviewed to gather data on the history of dog treatment with ectoparasiticides. The larval immersion test was used to assess the toxicological response of R. sanguineus s.s. to IVM. Dose-response mortality regressions, lethal concentrations (LC), and slope were calculated by probit analysis. The lowest LC concentrations were used to designate the reference susceptible population because a laboratory reference strain of R. sanguineus s.s. does not exist in Argentina. Compared with the most susceptible tick population in this study, six populations (66.66%) were classified as resistant to IVM. A clear interpopulation variation in the level of IVM resistance was present (resistance ratios at LC
50% ranged from 1.0 to 18.33 and at LC99% ranged from 1.0 to 8.96). In San Luis Province, all tick populations were classified as resistant. The highest level of IVM resistance (resistance ratio at LC50% :18.83 and LC99% :8.96) was found in a population of R. sanguineus s.s. from a rural area in the province of Buenos Aires. It is concluded that populations of R. sanguineus s.s. from dogs in three provinces of Argentina were resistant to IVM. Clear interpopulation variation in the level of IVM resistance was present., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2021
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9. Viruses that affect Argentinian honey bees (Apis mellifera).
- Author
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Salina MD, Garcia MLG, Bais B, Bravi ME, Brasesco C, Maggi M, Pecoraro M, Larsen A, Sguazza HG, and Reynaldi FJ
- Subjects
- Animals, Argentina, DNA Viruses genetics, DNA Viruses isolation & purification, Host-Pathogen Interactions, RNA Viruses genetics, RNA Viruses isolation & purification, Bees virology
- Abstract
Beekeeping is a widespread activity in Argentina, mainly producing honey that has gained both national and international recognition. There are more than 3,000,000 hives in the country, mainly concentrated in Buenos Aires Province (approximately 1,000,000 hives). In recent decades, worrying rates of hive loss have been observed in many countries around the world. In Latin America, the estimated loss of hives is between 13% (Peru and Ecuador) and 53% (Chile). Argentina had annual losses of 34% for the period of October 1, 2016 to October 1, 2017. The causes of these losses are not clear but probably involve multiple stressors that can act simultaneously. One of the main causes of loss of bee colonies worldwide is infestation by the ectoparasitic mite Varroa destructor in combination with viral infections. To date, 10 viruses have been detected that affect honey bees (Apis mellifera) in Argentina. Of these, deformed wing virus, sacbrood virus, acute bee paralysis virus, chronic bee paralysis virus, and Israeli acute bee paralysis can be transmitted by mites. Deformed wing virus and the AIK complex are the viruses most often associated with loss of hives worldwide. Considering that bee viruses have been detected in Argentina in several hymenopteran and non-hymenopteran insects, these hosts could act as important natural reservoirs for viruses and play an important role in their dispersal in the environment. Further studies to investigate the different mechanisms by which viruses spread in the environment will enable us to develop various strategies for the control of infected colonies and the spread of viruses in the habitat where they are found.
- Published
- 2021
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10. Intra-Colonial Viral Infections in Western Honey Bees ( Apis Mellifera ).
- Author
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Castelli L, Genchi García ML, Dalmon A, Arredondo D, Antúnez K, Invernizzi C, Reynaldi FJ, Le Conte Y, and Beaurepaire A
- Abstract
RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee ( Apis mellifera ) viruses at the colony level, the dynamics of virus infection within colonies remains poorly explored. In this study, the two main variants of the ubiquitous honey bee virus DWV as well as three major honey bee viruses (SBV, ABPV and BQCV) were analyzed from Varroa-destructor -parasitized pupae. More precisely, RT-qPCR was used to quantify and compare virus genome copies across honey bee pupae at the individual and subfamily levels (i.e., patrilines, sharing the same mother queen but with different drones as fathers). Additionally, virus genome copies were compared in cells parasitized by reproducing and non-reproducing mite foundresses to assess the role of this vector. Only DWV was detected in the samples, and the two variants of this virus significantly differed when comparing the sampling period, colonies and patrilines. Moreover, DWV-A and DWV-B exhibited different infection patterns, reflecting contrasting dynamics. Altogether, these results provide new insight into honey bee diseases and stress the need for more studies about the mechanisms of intra-colonial disease variation in social insects.
- Published
- 2021
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11. Lotmaria passim (Kinetoplastea: Trypanosomatidae) in honey bees from Argentina.
- Author
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Quintana S, Plischuk S, Brasesco C, Revainera P, Genchi García ML, Bravi ME, Reynaldi F, Eguaras M, and Maggi M
- Subjects
- Animals, Argentina, Bees parasitology, Trypanosomatina isolation & purification
- Abstract
Lotmaria passim (Kinetoplastea) is considered the most prevalent as well as the most virulent trypanosomatid associated to the European honey bee Apis mellifera. We used qPCR to screen for the presence of this parasite in 57 samples from ten Argentinian provinces, and were able to detect its presence throughout most of the country with 41% of the samples testing positive. In a retrospective analysis, we detected L. passim in 73% of honey bee samples from 2006 showing that this flagellate has been widely present in Argentina for at least ~15 years. Additionally, three primer sets for L. passim detection were compared, with the pair that produced smallest PCR product having the best detection capability. Finally, we also found L. passim DNA in 100% (n = 6) of samples of the mite Varroa destructor. The role of this ectoparasite in the lifecycle of Lotmaria, if any, remains unrevealed., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2021
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12. Host range of Triatoma virus does not extend to Aedes aegypti and Apis mellifera.
- Author
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Marti GA, Bonica MB, Susevich ML, Reynaldi F, Micieli MV, and Echeverría MG
- Subjects
- Aedes growth & development, Animals, Female, Larva growth & development, Larva virology, Pest Control, Biological, Aedes virology, Bees virology, Dicistroviridae physiology, Host Specificity
- Abstract
Vector control is the most effective method to prevent transmission of Chagas disease. Control is mostly made through chemical insecticides although they have negative impact on wild pollinators, such as bees. Reducing pesticide use through biological alternatives could minimize the damage to these beneficial insects. Triatoma virus (TrV) is a pathogen able to kill triatomines and thus a valid candidate to be used as biological control agent. In this study we evaluate the capacity of TrV to infect an important beneficial insect (Apis mellifera) as well as a plague insect (Aedes aegypti). Results indicate that TrV does not infect the bees or mosquitoes tested in this study. The possible specificity of TrV for kissing bugs reinforces the possible use of TrV as a biological control agent for triatomines., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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13. kMucormicosys due to Saksenaea vasiformis in a dog.
- Author
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Reynaldi FJ, Giacoboni G, Córdoba SB, Romero J, Reinoso EH, and Abrantes R
- Abstract
A 2-year-old female Border collie was examined for dermatitis with a partial alopecic zone around her left front member. Six months later the lesion became swollen, alopecic with ulcerated areas. Microscopy analysis of samples showed numerous non-septate, branching, thin-walled and irregular shaped hyphal elements. Fungal cultures and molecular studies identified Saksenaea vasiformis. Treatments with griseofulvin, itraconazole and surgical debridement were used, however, fourteen months later the dog was euthanatized because of the unfavorable clinical outcome.
- Published
- 2017
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14. Ascosphaera apis, the entomopathogenic fungus affecting larvae of native bees (Xylocopa augusti): First report in South America.
- Author
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Reynaldi FJ, Lucia M, and Genchi Garcia ML
- Subjects
- Animals, Argentina, Ascomycota ultrastructure, Bees growth & development, Hyphae ultrastructure, Larva microbiology, Species Specificity, Spores, Fungal, Ascomycota isolation & purification, Bees microbiology
- Abstract
Background: Nowadays several invertebrate pollinators of crops and wild plants are in decline as result of multiple and, sometimes, unknown factors; among them, the modern agricultural practices, pests and diseases are postulated as the most important factors. Bees of the genus Xylocopa are considered effective pollinators of passion fruit crops in tropical regions, as well as important pollinators in wild plants, but these bees are attacked by several pathogens that affect different stages in their life cycle. The fungal species of the genus Ascosphaera are commonly associated with social and solitary bee larvae causing chalkbrood disease., Aims: The aim of the present study was to demonstrate the presence of Ascosphaera apis affecting larvae of Xylocopa augusti in South America., Methods: For this purpose, A. apis was isolated from affected larvae in YGPSA medium. Final identification was run out by three techniques: (1) Microscopic examination of the hyphae and sizes of the fruiting bodies; (2) Mating test, and specific sexual compatibility test, and (3) PCR detection, using specific primers., Results: This study demonstrates for the first time the presence of A. apis affecting larvae of X. augusti in South America., Conclusions: The evidence of A. apis affecting the larvae of X. augusti, and the fact that the sharing of pathogens between different bee species has been underestimated, suggests the need for further epidemiological studies in order to determine not only the prevalence of this pathogen among wild pollinators, but also its relationship to the sudden collapse of honey bee colonies in this region., (Copyright © 2014 Revista Iberoamericana de Micología. Published by Elsevier Espana. All rights reserved.)
- Published
- 2015
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15. Simultaneous detection of bee viruses by multiplex PCR.
- Author
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Sguazza GH, Reynaldi FJ, Galosi CM, and Pecoraro MR
- Subjects
- Animals, Argentina, Bees virology, Multiplex Polymerase Chain Reaction methods, Veterinary Medicine methods, Virology methods, Viruses isolation & purification
- Abstract
Honey bee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. It is known that the consequence of the complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are detected frequently concomitantly in bee colonies. The aim of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of the most prevalent bee viruses. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of a particular virus as well as multiple virus infections in a single reaction tube. This method could be a helpful tool in the surveillance of the most frequently found bee viruses and to study the dynamics and the interactions of the virus populations within colonies., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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16. [Evaluation of the Epsilometer (Etest) method for the detection of tetracycline susceptibility in Paenibacillus larvae, the causal agent of American foulbrood disease of honeybees].
- Author
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Alippi AM, Reynaldi FJ, and López AC
- Subjects
- Animals, Bees microbiology, Gram-Positive Bacterial Infections, Microbial Sensitivity Tests methods, Anti-Bacterial Agents pharmacology, Paenibacillus drug effects, Tetracycline pharmacology
- Abstract
American foulbrood (AFB) is a bacterial disease caused by the spore-forming, grampositive bacterium Paenibacillus larvae, which affects honeybee broods worldwide. The aim of this work was to compare the Epsilometer test (Etest) to the agar dilution method for testing a collection of 22 P. larvae strains to tetracycline by using MYPGP and Iso- Sensitest agars. Results showed that a categorical agreement of 100% was found when using Iso-Sensitest, while a categorical agreement of 86.36% was found (with 3 minor errors) when MYPGP was tested. In conclusion, the Etest could be a rapid and reliable method for testing MIC values of tetracycline in P. larvae only when used in combination with Iso-Sensitest agar. Nevertheless, these results should be confirmed with future studies involving a larger number of isolates., (Copyright © 2013 Asociación Argentina de Microbiología. Publicado por Elsevier España. All rights reserved.)
- Published
- 2013
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17. First report of Israeli acute paralysis virus in asymptomatic hives of Argentina.
- Author
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Reynaldi FJ, Sguazza GH, Tizzano MA, Fuentealba N, Galosi CM, and Pecoraro MR
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- Animals, Argentina epidemiology, Colony Collapse epidemiology, Female, Reverse Transcriptase Polymerase Chain Reaction, Sampling Studies, Bees virology, Colony Collapse virology, Dicistroviridae isolation & purification
- Abstract
Honey bee mortality has recently been associated with Israeli acute paralysis virus (IAPV), a proposed etiological agent for a new syndrome known as Colony Collapse Disorder. Bees infected with this virus show shivering wings, progress into paralysis, and finally die outside the hive. During the last years, honey bee mortality became a serious problem for Argentinean beekeepers. We herein report the preliminary results of a survey carried out to detect IAPV in samples taken from several Argentine provinces, by using a reverse transcription Polymerase Chain Reaction assay. Our data indicate the existence of high frequency of IAPV in asymptomatic hives of Argentina.
- Published
- 2011
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18. First report of viral infections that affect argentine honeybees.
- Author
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Reynaldi FJ, Sguazza GH, Pecoraro MR, Tizzano MA, and Galosi CM
- Abstract
Honey is one of the most important agricultural products for export in Argentina. In fact, more than 3.5 million beehives and 50 000 beekeepers are related with this production, mainly located in Buenos Aires province. Honeybee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. It is known that the consequence of the complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are frequently detected concomitantly in bee colonies. We describe here the preliminary results of a survey of three honeybee-pathogenic viruses, acute bee paralysis viruses (ABPV), chronic bee paralysis viruses (CBPV) and Sacbrood viruses (SBV) detected during a screening of 61 apiaries located in the main honey producer province using a RT-PCR assay. This is the first molecular report of the presence of these viruses in Argentine apiaries., (© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.)
- Published
- 2010
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19. Effectiveness of tilmicosin against Paenibacillus larvae, the causal agent of American Foulbrood disease of honeybees.
- Author
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Reynaldi FJ, Albo GN, and Alippi AM
- Subjects
- Animals, Anti-Bacterial Agents adverse effects, Bees drug effects, Drug Resistance, Bacterial, Larva microbiology, Microbial Sensitivity Tests, Pupa microbiology, Tylosin adverse effects, Tylosin therapeutic use, Anti-Bacterial Agents therapeutic use, Bees microbiology, Gram-Positive Bacteria drug effects, Tylosin analogs & derivatives
- Abstract
American Foulbrood (AFB) of honeybees (Apis mellifera L.), caused by the Gram-positive bacterium Paenibacillus larvae is one of the most serious diseases affecting the larval and pupal stages of honeybees (A. mellifera L.). The aim of the present work was to asses the response of 23 strains of P. larvae from diverse geographical origins to tilmicosin, a macrolide antibiotic developed for exclusive use in veterinary medicine, by means of the minimal inhibitory concentration (MIC) and the agar diffusion test (ADT). All the strains tested were highly susceptible to tilmicosin with MIC values ranging between 0.0625 and 0.5 microg ml(-1), and with MIC(50) and MIC(90) values of 0.250 microg ml(-1). The ADT tests results for 23 P. larvae strains tested showed that all were susceptible to tilmicosin with inhibition zones around 15 microg tilmicosin disks ranging between 21 and 50mm in diameter. Oral acute toxicity of tilmicosin was evaluated and the LD(50) values obtained demonstrated that it was virtually non-toxic for adult bees and also resulted non-toxic for larvae when compared with the normal brood mortality. Dosage of 1000 mg a.i. of tilmicosin applied in a 55 g candy resulted in a total suppression of AFB clinical signs in honeybee colonies 60 days after initial treatment. To our knowledge, this is the first report of the effectiveness of tilmicosin against P. larvae both in vitro and in vivo.
- Published
- 2008
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20. Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees.
- Author
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Alippi AM, López AC, Reynaldi FJ, Grasso DH, and Aguilar OM
- Subjects
- Animals, Anti-Bacterial Agents therapeutic use, Bacillus drug effects, Bacillus genetics, Conjugation, Genetic drug effects, Conjugation, Genetic genetics, DNA, Bacterial chemistry, DNA, Bacterial genetics, Gram-Positive Bacterial Infections drug therapy, Microbial Sensitivity Tests veterinary, Oxytetracycline therapeutic use, Plasmids genetics, Polymerase Chain Reaction veterinary, Anti-Bacterial Agents pharmacology, Bacillus growth & development, Bees microbiology, Gram-Positive Bacterial Infections microbiology, Oxytetracycline pharmacology, Tetracycline Resistance genetics
- Abstract
Paenibacillus larvae is the causal agent of American Foulbrood (AFB) disease, the most virulent bacterial disease of honeybee (Apis mellifera L.) brood. Oxytetracycline is the main antibiotic used for prevention and control of AFB. Using the polymerase chain reaction, isolates were screened for the presence of the tetracycline resistance tet(K) and tet(L) determinants. Four isolates (5%), which correlated with the Tc-resistant phenotypes, were found to carry the tet(K) determinant, whereas none carried the tet(L) determinant. P. larvae cells were also screened for the presence of extrachromosomal DNA and evidence obtained that tetracycline resistance is plasmid-encoded. A few P. larvae isolates were found to be able to transfer the tet(K) determinant to Bacillus subtilis, suggesting that a conjugation mechanism may be involved in the transfer of the tetracycline-resistant phenotype. Minimum inhibitory concentrations to tetracycline were determined for 75 isolates of P. larvae from different geographical origins and found to range between 0.062 and 128 microg tetracyclineml(-1), with MIC(50) and MIC(90) values of 1 and 4, respectively. According to results from P. larvae populations, isolates could be considered as susceptible when their MICs were <4, intermediate for MICs values 4-8 and resistant for MICs > or = 16. To our knowledge, this is the first report of Tc(r)Paenibacillus species carrying a tet(K) gene, and also the first record of P. larvae strains carrying tet(K) determinants and its correlation with the presence of extrachromosomal DNA.
- Published
- 2007
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21. Inhibition of the growth of Paenibacillus larvae, the causal agent of American foulbrood of honeybees, by selected strains of aerobic spore-forming bacteria isolated from apiarian sources.
- Author
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Alippi AM and Reynaldi FJ
- Subjects
- Animals, Bacillus cereus growth & development, Bacterial Typing Techniques, Bacteriological Techniques, Honey microbiology, Larva growth & development, Larva microbiology, Bacillus classification, Bacillus growth & development, Bacteria, Aerobic physiology, Bees microbiology, Pest Control, Biological, Spores, Bacterial physiology
- Abstract
The bacterium Paenibacillus larvae, the causative agent of American foulbrood disease of honeybee larvae, occurs throughout the world and is found in many beekeeping areas of Argentina. The potential as biocontrol agents of antagonic aerobic spore-forming bacteria isolated from honey samples and other apiarian sources were evaluated. Each isolate was screened against one strain of Paenibacillus larvae (ATCC 9545) by using a perpendicular streak technique. Ten randomly selected bacterial strains from the group that showed the best antagonistic effect to P. larvae ATCC 9545 were selected for further study. These were identified as Bacillus subtilis (m351), B. pumilus (m350), B. licheniformis (m347), B. cereus (mv33), B. cereus (m387), B. cereus (m6c), B. megaterium (m404), Brevibacillus laterosporus (BLAT169), B. laterosporus (BLAT170), and B. laterosporus (BLAT171). The antagonistic strains were tested against 17 P. larvae strains from different geographical origins by means of a spot test in wells. The analysis of variance and posterior comparison of means by Tukey method (P < 0.01) showed that the best antagonists were B. megaterium (m404), B. licheniformis (m347), B. cereus (m6c), B. cereus (mv33), and B. cereus (m387).
- Published
- 2006
- Full Text
- View/download PDF
22. In vitro and in vivo susceptibility of the honeybee bacterial pathogen Paenibacillus larvae subsp. larvae to the antibiotic tylosin.
- Author
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Alippi AM, Albo GN, Reynaldi FJ, and De Giusti MR
- Subjects
- Animals, Anti-Bacterial Agents therapeutic use, Argentina, Bacillus growth & development, Bacillus isolation & purification, Bacterial Infections drug therapy, Bacterial Infections microbiology, Dose-Response Relationship, Drug, Female, Microbial Sensitivity Tests, Statistics, Nonparametric, Tylosin therapeutic use, Anti-Bacterial Agents pharmacology, Bacillus drug effects, Bacterial Infections veterinary, Bees microbiology, Tylosin pharmacology
- Abstract
The minimal inhibitory concentrations (MICs) of tylosin were determined to 67 strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood (AFB) disease, from different geographical origins. MIC values obtained ranged from 0.0078 to 0.5 microg/ml. These very low values imply that no resistance to tylosin was found in any isolate of the Foulbrood pathogen. The measurement of diseased larvae with AFB-clinical symptoms in three different field studies demonstrated that tylosin treatment could be effective in vivo. No negative effects in colonies were noted at any dosage rates or forms of application. These studies demonstrate that tylosin, as tartrate, can be used to treat AFB in honeybee colonies.
- Published
- 2005
- Full Text
- View/download PDF
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