1. Comparatine analysis of cytophenotypes of cells of mesenchymal lineage isolated from human tissues.
- Author
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Suzdal'tseva YG, Burunova VV, Petrakova NV, Vakhrushev IV, Yarygin KN, and Yarygin VN
- Subjects
- Bone Marrow Cells cytology, Bone Marrow Cells metabolism, Cell Lineage, Female, Fibroblasts cytology, Fibroblasts metabolism, Humans, Immunohistochemistry, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Proteome biosynthesis, Skin cytology, Stem Cells metabolism, Mesoderm cytology, Stem Cells cytology, Umbilical Cord cytology
- Abstract
The expression of cytoplasmic and surface proteins in cultured human skin fibroblasts, human umbilical cord cells obtained after normal delivery on gestation week 38-40, and mesenchymal bone marrow stem cells was compared by the methods of immunocytochemistry and flow cytofluorometry. Bone marrow mesenchymal stem cells expressed a great variety of marker proteins typical of stem and progenitor cells and did not express proteins typical of differentiated cells. Fibroblast-like umbilical cord cells expressed markers of both stem cells and differentiated cells. Fibroblasts of dermal origin were characterized by intensive expression of proteins typical of differentiated cells.
- Published
- 2007
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