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In this study, odor characteristics and phytoplankton composition were systematically investigated in two winter periods in a reservoir with fishy odor in north China. Ten potential fishy odor-producing algae were isolated and odorant-producing potentials were evaluated. Olfactometry profile and odorant composition of water samples were analyzed using GC-Olfactometry combined with GC × GC-TOFMS. The results showed that 2,4-heptadienal and hexanal were major fishy odor contributors. The abundance of Uroglena sp., Synura sp. and Peridinium sp. was negatively correlated with total dissolved organic carbon, ammonia nitrogen, and nitrate, illustrating nutrient level might be major drivers for the succession of fishy odor-producing algae. Dinobryon sp. and Uroglena sp. made the greatest contribution to fishy odor, followed by Peridinium sp., Synura sp., and Ochromonas sp. Fishy odor in 2016 winter and the early of 2017 winter was mainly caused by Dinobryon sp., while Uroglena sp. contributes mostly in March in 2017 winter. This study demonstrates the main odorants and algae causing fishy odor in reservoir, which will provide a scientific basis for the management of seasonal fishy odor problems in water source., Competing Interests: Declaration of competing interest The authors declare no competing financial interest., (Copyright © 2023. Published by Elsevier B.V.)

Herbicide pollution is a main form of water pollution. As a result of additional harms to other non-target organisms, it threatens the function and structure of ecosystems. Previous researches mainly focused on the assessment of the toxicity and ecological effect of herbicides on monotrophic organisms. Responses of mixotrophs as an important component of functional groups are rarely understood in contaminated waters, although their metabolic plasticity and unique ecological functions in ecosystem stability are a major concern. This work aimed to investigate the trophic plasticity of mixotrophic organisms in atrazine-contaminated waters, and a primarily heterotrophic Ochromonas was used as the tested organism. Results showed that the herbicide atrazine significantly inhibited the photochemical activity and impaired the photosynthetic machine of Ochromonas, and photosynthesis activated by light was sensitive to atrazine. However, phagotrophy was unaffected by atrazine and closely correlated with growth rate, indicating that heterotrophy helped population maintenance during herbicide exposure. Mixotrophic Ochromonas upregulated the gene expression level involved in photosynthesis, energy synthesis, and antioxidation to adapt to increasing atrazine after long-term exposure. Compared with bacterivory, herbivory increased atrazine tolerance of photosynthesis under mixotrophic status. This study systematically illustrated the mechanism by which mixotrophic Ochromonas respond to the herbicide atrazine at population, photochemical activity, morphology, and gene expression levels and demonstrated the potential effect of atrazine on the metabolic flexibility and ecological niches of mixotrophs. These findings will provide important theoretical reference for governance and management decision-making in contaminated environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Mixotrophs, organisms that combine photosynthesis and heterotrophy to gain energy, play an important role in global biogeochemical cycles. Metabolic theory predicts that mixotrophs will become more heterotrophic with rising temperatures, potentially creating a positive feedback loop that accelerates carbon dioxide accumulation in the atmosphere. Studies testing this theory have focused on phenotypically plastic (short-term, non-evolutionary) thermal responses of mixotrophs. However, as small organisms with short generation times and large population sizes, mixotrophs may rapidly evolve in response to climate change. Here, we present data from a 3-year experiment quantifying the evolutionary response of two mixotrophic nanoflagellates to temperature. We found evidence for adaptive evolution (increased growth rates in evolved relative to acclimated lineages) in the obligately phototrophic strain, but not in the facultative phototroph. All lineages showed trends of increased carbon use efficiency, flattening of thermal reaction norms, and a return to homeostatic gene expression. Generally, mixotrophs evolved reduced photosynthesis and higher grazing with increased temperatures, suggesting that evolution may act to exacerbate mixotrophs' effects on global carbon cycling., (© 2022 The Authors. Global Change Biology published by John Wiley & Sons Ltd.)

Fucoxanthin (Fx) has attracted great interest due to its remarkable biological activities such as antioxidant and anti-obesity, and its increasing demands in biopharmaceutical and cosmetic fields. However, its commercial production is limited by low yield and high cost. In this study, we isolated and identified a species of golden algae (Ochromonas sp.) capable of engulfing Microcystis aeruginosa (M. aeruginosa) and accumulating Fx. After 72 h mixotrophic cultivation of Ochromonas sp. and M. aeruginosa, the algal culture changed from green to yellow-brown, and the content of Fx and the daily production rate were up to 11.58 mg g -1 , and 1.315 mg L -1 d -1 , respectively. The utilization rate of M. aeruginosa was 527.27 fg cell -1 . This study will not only provide a new thought to produce Fx in an efficient, low-cost, and sustainable way but an innovative method for the control and treatment of harmful cyanobacterial blooms from eutrophic freshwaters as well., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

"Spumella"-like flagellates describes similar or even indiscernible colourless non-scaled chrysophytes which are important bacterivores common in different aquatic ecosystems. Recently, phylogenetic analyses revealed a high taxonomic diversity of these flagellates leading to the description of several new genera and species. Our present work on the functional group of pelagic bacterivorous chrysomonads from different water bodies resulted in an extended taxonomic analysis among chrysophytes unveiling yet undescribed genera and species pointing to the high hidden diversity of bacterivores in the pelagial of freshwaters. On the basis of phylogenetic analyses, we describe four new genera Atacamaspumella, Chlorospumella, Pseudapoikia, and Vivaspumella and a new species of the recently described genus Poteriospumella. Beside this, we redescribe the species Ochromonas vasocystis Doflein, 1923 to Poteriospumella vasocystis comb. nov. substantiated on the high sequence similarity with Poteriospumella lacustris Boenigk et Findenig and Poteriospumella maldiviensis nov. sp., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier GmbH. All rights reserved.)

Cyanobacterial blooms are increasingly threatening the aquatic ecosystem functioning as a result of the global warming and eutrophication. The "top-down" control of cyanobacteria from consumers like the protozoans shows great potential because of the effectiveness and environment-friendliness. To reveal how the nutrition availability and elevated temperature affect the cyanobacteria removal through protozoans grazing, we grew the toxic Microcystis aeruginosa and the mixotrophic Ochromonas gloeopara in monocultures and cocultures at environmentally relevant nitrogen levels (0.5-8.0 mg L -1 ) under 25 °C and 30 °C, respectively. The growth of M. aeruginosa in monocultures was significantly enhanced as nitrogen concentration and temperature rose, partially benefitting from the promoted photosynthesis. By contrast, nitrogen availability affected neither the photoautotrophic growth nor the feeding on Microcystis of the mixotrophic O. gloeopara, but high temperature induced the mixotroph to be more heterotrophic as evidenced by the suppressed photosynthesis but strengthened feeding activity. Accordingly, the M. aeruginosa removal through O. gloeopara grazing in cocultures was delayed with increasing nitrogen, which, however, was sharply accelerated by elevated temperature. Based on the Gaussian models fitting, the theoretical time that the Microcystis was removed at 25 °C was prolonged from about 7.5 days to 10 days with increased nitrogen, but it was reduced to less than 4.6 days in all groups at 30 °C. While the intensity of Microcystis blooms is strongly positively correlated to the nutrition availability and temperature, the present study provided references for the practical application of Microcystis removal through grazing outdoors., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Removal of harmful cyanobacteria is an extremely urgent task in global lake management and protection. Conventional measures are insufficient for simultaneously removing cyanobacteria and hazardous cyanotoxin, efficient and environmental-friendly measures are therefore particularly needed. Herbivorous protozoa have great potentials in controlling algae, however, large-sized colonial Microcystis is inedible for protozoa, which is a central problem to be solved. Therefore, in present study, a measure of protozoa grazing assisted by ultrasound was investigated in laboratory scale for eliminating harmful colonial Microcystis. The results showed that with ultrasound power and time increasing, the proportion of unicellular Microcystis increased significantly. With Ochromonas addition, approximately 80% of colonial Microcystis and microcystin was removed on day 4 under ultrasound power of 100 W for 15 min, while Ochromonas only reduced Microcystis by less than 20% without assistance of ultrasound. Moreover, when directly exposed to low-intensity ultrasound, Ochromonas showed strong resistance to ultrasound and were not inhibited in grazing Microcystis. Overall, ultrasound increases edible food for protozoa via collapsing Microcystis colonies and assists Ochromonas to remove Microcystis, thus intermittently collapsing colonial Microcystis using low-intensity ultrasound can significantly improve the removal efficiency of Microcystis by protozoa grazing, which provided a new insight in controlling harmful colonial Microcystis., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Phagotrophic algae can consume bacteria that are the predominant microorganisms present in the waste activated sludge (WAS) generated from municipal wastewater treatment processes. In this study, we developed a combined ultrasonication-phagotrophic algal process for WAS conversion. The ultrasonic pretreatment released small volatile solids (VS) including bacteria from WAS flocs. A phagotrophic alga Ochromonas danica then grew by consuming more than 80% of the released VS, with approximately 30% (w/w) algal cell yield. The process reduced the overall WAS VS by 42.4% in 1 day, comparing very favorably with the 27% reduction in 10 days by aerobic digestion. For stabilizing the solids remaining from the ultrasonic step, the total oxygen uptake required was 65%-92% lower than that for the original WAS, indicating substantially reduced aeration cost. Overall, this novel process enhanced the WAS digestion at lower energy requirements and produced microalgae for other potential uses. © 2021 Water Environment Federation PRACTITIONER POINTS: At least 80% of released VS from WAS can be processed by phagotrophic algae. Significant amounts of algae can be produced from WAS. Ultrasonication-phagotrophic algal process can make sludge management more sustainable., (© 2021 Water Environment Federation.)

Microplastics with different sizes exist widely in fresh waters, which may affect the interspecific dynamics between predator and prey. The flagellate Ochromonas gloeopara can efficiently eliminate Microcystis aeruginosa and degrade microcystins, which shows great potential for controlling harmful Microcystis. In order to evaluate the effects of microplastics on O. gloeopara eliminating Microcystis, we designed an experiment of O. gloeopara feeding on Microcystis under different sizes (0.07 and 3 μm) and concentrations (0, 0.4, 0.8, 1.6, and 2.0 mg L -1 ) of microplastics. The results showed that maximum abundance of M. aeruginosa decreased significantly with addition of microplastics, regardless of the size and concentration. O. gloeopara can ingest the microplastics and suffer from their adverse effects. The maximum abundance of O. gloeopara decreased with enhancing concentrations of 3 μm microplastics during the process of O. gloeopara eliminating M. aeruginosa, whereas 0.07 μm microplastics did not affect the growth of O. gloeopara obviously. During the period of exposure under microplastics, clearance rate of O. gloeopara on M. aeruginosa decreased with the increasing concentrations of microplastics. Specially, 3 μm microplastics had a stronger reduction on clearance rate of O. gloeopara. The time to M. aeruginosa extinction was prolonged with the increasing concentrations of microplastics in both sizes. Comparatively speaking, 3 μm microplastics had a stronger delayed effect on the removal of Microcystis. These findings suggest that microplastics can interfere with protozoa eliminating toxic Microcystis, which may aggravate their adverse impacts on aquatic ecosystem., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Cadmium (Cd) is one of the predominant anthropogenic pollutants in aquatic systems. As Cd has negative effects on species at all trophic levels, the community composition in aquatic habitats can be changed as a result of Cd stress. The response of mixotrophic protists to environmental stressors is particularly important as they act as both producers and consumers in complex planktonic communities. In this study, we used mixotrophic Ochromonas gloeopara to study its growth and photosynthetic responses to Cd, and specially focused on the effects of initial Cd concentrations and nutrient levels on its capacity to remove Cd. Results showed that when Cd concentration reached 0.5 mg L -1 , the growth rate and carrying capacity were significantly inhibited, whereas the photosynthesis was markedly decreased when Cd concentration reached 0.15 mg L -1 . Moreover, under Cd concentration 0.15, 0.5, 0.9, 1.6, and 2.0 mg L -1 , the removal efficiencies of Cd by O. gloeopara were 83.2%, 77.7%, 74.6%, 70.1%, and 68.8%, respectively. The increase of nitrogen did not cause significant effect on the removal capacity of Cd by O. gloeopara, but increased concentration of phosphorus significantly enhanced the removal capacity of Cd. Our findings indicated that the mixotrophic O. gloeopara has strong tolerance and capacity to remove Cd, and increasing concentration of phosphorus can increase its removal capacity, suggesting that O. gloeopara has great potential application value in mitigating Cd pollution in waters., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Mixotrophs account for a high proportion (occasionally up to 80%) of the phytoplankton biomass. Chrysophyte is one major component of mixotrophs. Because of their possible toxicity and linkage between microbial community and higher trophic levels, the effect of mixotrophic golden algae on potential grazers received much attention. The present study investigated the effect of Ochromonas gloeopara at different proportions in diet (combined with Scenedesmus obliquus) on the life history of Daphnia similoides sinensis. Results showed that osmotrophically grown O. gloeopara in light produced fish toxins and hemolysins, and negatively influenced the survival and reproduction of D. similoides sinensis. The mortality of the cladoceran increased as the proportion of O. gloeopara in food increased. The D. similoides sinensis could not reproduce throughout the life when Ochromonas comprised above 35%. When fed foods containing 15% of Ochromonas, the time to first brood of D. similoides sinensis was prolonged, together with the reduced number of offspring in first brood and total number of broods. Replacement by 100% S. obliquus delayed the time to death, but did not improve the reproduction of Daphnia. The present study indicated the strong inhibitory effect of O. gloeopara on D. similoides sinensis, and underlined the importance of evaluating its ecological role in aquatic ecosystems.

Driven by global warming and eutrophication, outbreaks of cyanobacterial blooms have severely impacted ecosystem stability and water safety. Of the organisms used to control cyanobacteria, protozoa can highly resist cyanotoxins, efficiently control cyanobacterial populations, and show considerably different feeding strategies from those of metazoans. Thus, protozoa have great potential to control harmful cyanobacteria and improve phytoplankton composition in eutrophic waters. To evaluate the actual effects of protozoa in controlling cyanobacteria and improving the phytoplankton community structure in the field, an in situ microcosm study was performed using a flagellate Ochromonas gloeopara that ingests Microcystis . Results showed that adding Ochromonas reduced the cyanobacterial populations and increased the chlorophyte and diatom proportions. Furthermore, the species richness and diversity of the phytoplankton community were enhanced in microcosms with Ochromonas . Additionally, there was a gradual increase in the chlorophyte population in the unicellular Microcystis control, while Ochromonas addition significantly accelerated the replacement of dominant species. This study was the first to show the practical effects of protozoa on controlling cyanobacteria in the field, highlighting that a reduction in in situ cyanobacteria via protozoa can improve the phytoplankton community structure, dredge the toxic cyanobacteria-dominated microbial food web, and mitigate harmful cyanobacteria risks in fresh waters.

Resource competition theory predicts that when two species compete for a single, finite resource, the better competitor should exclude the other. However, in some cases, weaker competitors can persist through intraguild predation, that is, by eating their stronger competitor. Mixotrophs, species that meet their carbon demand by combining photosynthesis and phagotrophic heterotrophy, may function as intraguild predators when they consume the phototrophs with which they compete for light. Thus, theory predicts that mixotrophy may allow for coexistence of two species on a single limiting resource. We tested this prediction by developing a new mathematical model for a unicellular mixotroph and phytoplankter that compete for light, and comparing the model's predictions with a laboratory experimental system. We find that, like other intraguild predators, mixotrophs can persist when an ecosystem is sufficiently productive (i.e., the supply of the limiting resource, light, is relatively high), or when species interactions are strong (i.e., attack rates and conversion efficiencies are high). Both our mathematical and laboratory models show that, depending upon the environment and species traits, a variety of equilibrium outcomes, ranging from competitive exclusion to coexistence, are possible., (© 2019 by the Ecological Society of America.)

In this study waste activated sludge (WAS) was sonicated to release bacteria-sized volatile solids (VS) from flocs, after initial pH adjustment to 10 for higher energy efficiency. The released VS supported growth of phagotrophic alga Ochromonas danica. Initial-rate growth experiments confirmed the Monod-type kinetics but the specific cell growth rate, μ, correlated with the prey-to-predator ratio, i.e., the ratio of (fed VS concentration)-to-(initial O. danica concentration), significantly better than with the VS alone, as the typical Monod dependency on soluble substrates. The best-fit kinetics had the following parameters: μ max  = 0.198 h -1 and K M  = 1.056 (g-VS/g-algae). Post-sonication reflocculation could render particles too large to ingest by O. danica; therefore, pH and VS effects on reflocculation were investigated. Batch cultivations were then conducted in fermentors at pH 5, under nonsterile conditions. Algae number reached 8.86 × 10 10  L -1 after 20 h, corresponding to ∼2.3 g/L dry-weight and volumetric algae productivity of 2.8 g/L-day. VS reduction was 38%, giving an O. danica VS yield of 44.5%. The ultrasonication-algae process can be used to produce algae while achieving at least partial WAS treatment., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Cyanobacterial blooms, caused by eutrophication and climate warming, exert severely negative effects on aquatic ecosystem. Some species of protozoans can graze on toxic cyanobacteria and degrade microcystins highly efficiently, which shows a promising way to control the harmful algae. However, in the field, many different species of algae coexist with Microcystis and may affect protozoans eliminating Microcystis. Therefore, in this study, we assessed the impacts of chlorophytes, a type of beneficial algae for zooplankton and common competitors of cyanobacteria, on flagellate Ochromonas eliminating toxin-producing Microcystis at different temperatures. Our results showed that Ochromonas still eliminated Microcystis population and degraded the total microcystins with the addition of chlorophytes, although the time of eliminating Microcystis was prolonged and temperature-dependent. Additionally, in the grazing treatments, chlorophytes populations gradually increased with the depletion of Microcystis, whereas Microcystis dominated in the mixed algal cultures without Ochromonas. The findings indicated that although chlorophytes prolong mixotrophic Ochromonas eliminating Microcystis, the flagellate grazing Microcystis helps chlorophytes dominating in the primary producers, which is significant in improving water quality and reducing aquatic ecosystem risks., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Toxic Microcystis bloom is a tough environment problem worldwide. Microcystin is highly toxic and is an easily accumulated secondary metabolite of toxic Microcystis that threatens water safety. Biodegradation of microcystin by protozoan grazing is a promising and efficient biological method, but the mechanism in this process is still unclear. The present study aimed to identify potential pathways involved in resisting and degrading microcystin in flagellates through transcriptomic analyses. A total of 999 unigenes were significantly differentially expressed between treatments with flagellates Ochromonas fed on microcystin-producing Microcystis and microcystin-free Microcystis. These dysregulated genes were strongly associated with translation, carbohydrate metabolism, phagosome, and energy metabolism. Upregulated genes encoding peroxiredoxin, serine/threonine-protein phosphatase, glutathione S-transferase (GST), HSP70, and O-GlcNAc transferase were involved in resisting microcystin. In addition, genes encoding cathepsin and GST and genes related to inducing reactive oxygen species (ROS) were all upregulated, which highly probably linked with degrading microcystin in flagellates. The results of this study provided a better understanding of transcriptomic responses of flagellates to toxic Microcystis as well as highlighted a potential mechanism of biodegrading microcystin by flagellate Ochromonas, which served as a strong theoretical support for control of toxic microalgae by protozoans.

A mixed cyanobacterial-mixotrophic algal population, dominated by the filamentous cyanobacterium Leptolyngbya sp. and the microalga Ochromonas (which contributed to the total photosynthetic population with rates of less than 5%), was studied under non-aseptic conditions for its efficiency to remove organic and inorganic compounds from different types of wastes/wastewaters while simultaneously producing lipids. Second cheese whey, poplar sawdust, and grass hydrolysates were used in lab-scale experiments, in photobioreactors that operated under aerobic conditions with different initial nutrient (C, N and P) concentrations. Nutrient removal rates, biomass productivity, and the maximum oil production rates were determined. The highest lipid production was achieved using the biologically treated dairy effluent (up to 14.8% oil in dry biomass corresponding to 124 mg L -1 ) which also led to high nutrient removal rates (up to 94%). Lipids synthesized by the microbial consortium contained high percentages of saturated and mono-unsaturated fatty acids (up to 75% in total lipids) for all the substrates tested, which implies that the produced biomass may be harnessed as a source of biodiesel.

This study aimed to investigate the influence of temperature on the ability of the mixotrophic flagellate Ochromonas to eliminate a toxic Microcystis population and degrade microcystins. We exposed Microcystis to cultures with or without Ochromonas YZ1 at 20, 25, and 30 °C for 10 days. Results showed that increased temperature promoted the growth of Ochromonas YZ1 and Microcystis, with the latter achieving high abundance without grazing. With increased temperature, Ochromonas YZ1 clearance rate increased, and Microcystis populations were earlier eliminated. Importantly, Ochromonas YZ1 degraded both intracellular and extracellular microcystins by grazing effects. The reduction ratios of Microcystis abundances and microcystins were both approximately 100% after 6 days at high temperature. In addition, more microcystins were released outside at 20 °C than at the higher temperatures. Overall, this study showed that high temperature favors elimination of toxin-producing Microcystis and degradation of microcystins by mixotrophic Ochromonas., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Background: Ochromonas is a genus of mixotrophic chrysophytes that is found ubiquitously in many aquatic environments. Species in this genus can be important consumers of bacteria but vary in their ability to perform photosynthesis. We studied the effect of light and bacteria on growth and gene expression of a predominantly phagotrophic Ochromonas species. Axenic cultures of Ochromonas sp. were fed with heat-killed bacteria (HKB) and grown in constant light or darkness. RNA was extracted from cultures in the light or in the dark with HKB present (Light + HKB; Dark + HKB), and in the light after HKB were depleted (Light + depleted HKB)., Results: There were no significant differences in the growth or bacterial ingestion rates between algae grown in light or dark conditions. The availability of light led to a differential expression of only 8% of genes in the transcriptome. A number of genes associated with photosynthesis, phagotrophy, and tetrapyrrole synthesis was upregulated in the Light + HKB treatment compared to Dark + HKB. Conversely, the comparison between the Light + HKB and Light + depleted HKB treatments revealed that the presence of HKB led to differential expression of 59% of genes, including the majority of genes involved in major carbon and nitrogen metabolic pathways. Genes coding for unidirectional enzymes for the utilization of glucose were upregulated in the presence of HKB, implying increased glycolytic activities during phagotrophy. Algae without HKB upregulated their expression of genes coding for ammonium transporters, implying uptake of inorganic nitrogen from the culture medium when prey were unavailable., Conclusions: Transcriptomic results agreed with previous observations that light had minimal effect on the population growth of Ochromonas sp. However, light led to the upregulation of a number of phototrophy- and phagotrophy-related genes, while the availability of bacterial prey led to prominent changes in major carbon and nitrogen metabolic pathways. Our study demonstrated the potential of transcriptomic approaches to improve our understanding of the trophic physiologies of complex mixotrophs, and revealed responses in Ochromonas sp. not apparent from traditional culture studies.

Two-dimensional (2-D) protein analysis of the mastigoneme fraction of the chromophyte alga Ochromonas danica E. G. Pringsh. showed the presence of several component proteins of the tubular mastigoneme. Adding to the reported gene Ocm1, three new genes (Ocm2, Ocm3, and Ocm4) belonging to the Ocm gene family were isolated using degenerate primers designed from predicted Ocm1 amino acid sequences. The predicted polypeptides encoded by Ocm2, Ocm3, and Ocm4 were smaller in size than Ocm1. However, they shared four highly conserved, cysteine-rich, epithelial growth factor (EGF)-like motifs, potentially involved in protein-protein interaction. In addition, Ocm2, Ocm3, and Ocm4 showed homology to the SIG protein family in the centric diatom Thalassiosira weissflogii (Grunow) Fryxell et Hasle, which is up-regulated during early stages of sexual reproduction. Immunofluorescence analysis with a polyclonal antibody against the partial amino acid sequences of Ocm2, Ocm3, and Ocm4 showed that Ocm2 and Ocm3 were located in the basal segment region of mastigonemes attached on the surface of the anterior flagellum, and that Ocm4 was located within the tubular shaft portion similar to Ocm1., (© 2009 Phycological Society of America.)

When dark-grown cells of Ochromonas danica are placed in the light, the amount of chlorophyll a per cell increases 82-fold; the content of carotenoid pigment, 24-fold. Concomitantly with this increase in chlorophyll and carotenoid pigment, the small proplastid of dark-grown cells develops into a large lamellate chloroplast. During the first 12 hours in the light, vesicles appear within the loose clusters of dense chloroplast granules, enlarge, align themselves into rows (plates in three dimensions), and fuse into discs. Double discs may form from the more or less simultaneous fusion of two adjacent plates of vesicles or by the addition of vesicles to an already formed single disc. Three-disc bands arise by the addition of a disc to an already formed two-disc band through the approach and fusion of more vesicles. After 24 hours in the light, most of the chloroplast bands contain three discs, but the chloroplasts are still small. After 48 hours in the light, almost all the cells contain full-sized chloroplasts with a full complement of three-disc bands. However, at this time the amount of chlorophyll a and carotenoid pigment is only one-half of maximum. During the next 3 days in the light, as the number of chlorophyll and carotenoid molecules per chloroplast approximately doubles, there is a compression of the discs in each band (from 180 to 130 A) and a precise alignment of their membranes. Changes also occur in the nucleus when dark-grown cells are placed in the light. There is an increase in the number of small nucleolar bodies, many of which lie directly against the nuclear envelope, and in a few cells a dense mass of granules is seen between the two membranes of the nuclear envelope.