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Soil moisture is a key factor for vegetation restoration in arid and semi-arid regions. Clarifying the vertical characteristics of soil moisture in artificial forests on a regional scale and its response mechanisms can benefit for land use management in water-deficient areas such as the Loess Plateau. The study targets Robinia pseudoacacia on the Loess Plateau with a meta-analysis based on 790 soil moisture data points abstracted from 35 published papers. The results show that extensive cultivation of R pseudoacacia on the Loess Plateau leads to a significant reduction in soil moisture (P < 0.05). Soil moisture decreases significantly with growth of trees, especially between 400 and 500 cm soil layers. Soil moisture increases with the hydrothermal gradient. The results indicate that intensive afforestation activities in high temperature and rainy areas still significantly consume deep soil moisture. The main reason is that the impact of hydrothermal factors on soil moisture is significant between 0 and 200 cm soil layers and decreases with increasing soil depth. However, the continuous depletion of deep soil moisture leads to insignificant differences in soil moisture responses under different topographical conditions in the region. Therefore, neglecting the impact of forest age and hydrothermal factors on soil moisture in afforestation activities, the excessive water consumption by R pseudoacacia during growth poses potential risks to the ecological environment of the Loess Plateau. This study provides references for knowledge on water relating problems and sustainable management of artificial forests in arid and semi-arid areas., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

China is not only the first reported place of the COVID-19 pandemic but also is the biggest microplastic emitter in the world. Nevertheless, the impact of the COVID-19 pandemic on microplastic pollution in the watersheds of China remains poorly understood. To address this, the present study conducted a data mining and multivariate statistical analysis based on 8898 microplastic samples from 23 Chinese watershed systems before and during the COVID-19 pandemic. The results showed that the COVID-19 pandemic extensively affected the abundance, colors, shapes, polymer types, and particle sizes of microplastic in Chinese watershed systems. Before and during the COVID-19 pandemic, 77.27 % of the Chinese watershed systems observed increased microplastic abundance. Moreover, the COVID-19 pandemic itself, natural conditions (such as altitude and weather), and anthropogenic factors (such as civil aviation throughput) are highly intertwined, jointly impacting the microplastic in the watersheds of China. From the perspective of ecological risks, the COVID-19 pandemic was more likely to aggravate the microplastic pollution in the middle and down reaches of the Yangtze River Watersheds. Overall, whether before or during the COVID-19 pandemic, the main watershed systems of China still stayed at a high pollution level, which rang the alarm bell that watershed systems of China had been at serious ecological risk accused of microplastic contamination., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Soil respiration (R s ) is projected to be substantially affected by climate change, impacting the storage, equilibrium, and movement of terrestrial carbon (C). However, uncertainties surrounding the responses of R s to climate change and soil nitrogen (N) enrichment are linked to mechanisms specific to diverse climate zones. A comprehensive meta-analysis was conducted to address this, evaluating the global effects of warming, increased precipitation, and N enrichment on R s across various climate zones and ecosystems. Data from 123 studies, encompassing a total of 10,377 worldwide observations, were synthesized for this purpose. Annual R s were modeled and their uncertainties were associated with a 1-km 2 resolution global R s database spanning from 1961 to 2022. Calibrating R s using ensemble machine learning (EML) and employing 10-fold cross-validation, 13 environmental covariates were utilized. The meta-analysis findings revealed an upsurge in R s rates in response to warming, with tropical, arid, and temperate climate zones exhibiting increases of 12 %, 13 %, and 16 %, respectively. Furthermore, increased precipitation led to stimulated R s rates of 11 % and 9 % in tropical and temperate zones, respectively, while N deposition affected R s in cold (+6 %) and tropical (+5 %) climate zones. The machine learning technique estimated the global soil respiration to range from 91 to 171 Pg C yr -1 , with an average R s of 700 ± 300 g C m -2  yr -1 . The values ranged between 314 and 2500 g C m -2  yr -1 , with the lowest and highest values observed in cold and tropical zones, respectively. Spatial variation in R s was most pronounced in low-latitude areas, particularly in tropical rainforests and monsoon zones. Temperature, precipitation, and N deposition were identified as crucial environmental factors exerting significant influences on R s rates worldwide. These factors underscore the interconnectedness between climate and ecosystem processes, therefore requiring explicit considerations of different climate zones when assessing responses of R s to global change., Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Currently, tire wear particles (TWPs), a typical type of black microplastics (MPs), are frequently overlooked as the major source of MPs in aquatic environments. TWPs are widely distributed and exhibit complex environmental behaviors. However, how natural forces affect the aging and release behavior of TWPs at the nano(micro)scale remains inadequately explored. This study systematically investigated the aging behavior and mechanism of TWPs under the action of simulated natural light and high-temperature in both dry and wet environments, as well as the effect of aging treatment on the released leachate. The findings demonstrated that aging treatment significantly altered the physicochemical properties of TWPs, including chain scission and surface oxidation, and facilitated the release of heavy metals and organic additives in the meantime. In particular, the leaching concentration of Zn exhibited a positive linear relationship with exposure time. In the thermal-aging process, the oxidation of TWPs was primarily caused by superoxide anion (O 2 •- ). During the photo-aging exposure, TWPs exhibited heightened electron-donating capacity, resulting in the formation of more O 2 •- and singlet oxygen ( 1 O 2 ) to attack TWPs. Moreover, the analysis of leachate produced under light and high-temperature conditions suggested that heavy metals exerted low ecological risks in water. Nonetheless, the photo-aging process enhanced the toxicity of released leachate to L929 cells, which could be attributed to highly toxic additive transformation products (such as HMMM-411 and 6PPD-Q) and more heavy metals. These findings shed light on the fate of TWPs and the ecological risks posed by aged TWPs in aquatic environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Freeze-thaw (FT) events profoundly perturb the biochemical processes of soil and water in mid- and high-latitude regions, especially the riparian zones that are often recognized as the hotspots of soil-water interactions and thus one of the most sensitive ecosystems to future climate change. However, it remains largely unknown how the heterogeneously composed and progressively discharged meltwater affect the biochemical cycling of the neighbor soil. In this study, stream water from a valley in the Chinese Loess Plateau was frozen at -10°C for 12 hours, and the meltwater (at +10°C) progressively discharged at three stages (T1 ∼ T3) was respectively added to rewet the soil collected from the same stream bed (Soil+T1 ∼ Soil+T3). Our results show that: (1) Approximately 65% of the total dissolved organic carbon and 53% of the total NO 3 - -N were preferentially discharged at the first stage T1, with enrichment ratios of 1.60 ∼ 1.94. (2) The dissolved organic matter discharged at T1 was noticeably more biodegradable with significantly lower SUVA 254 but higher HIX, and also predominated with humic-like, dissolved microbial metabolite-like, and fulvic acid-like components. (3) After added to the soil, the meltwater discharged at T1 (e.g., Soil+T1) significantly accelerated the mineralization of soil organic carbon with 2.4 ∼ 8.07-folded k factor after fitted into the first-order kinetics equation, triggering 125 ∼ 152% more total CO 2 emission pulses. This reveals a common but long-ignored phenomenon in cold riparian zones, where progressive freeze-thaw can partition and thus shift the DOM compositions in stream water over melting time, and in turn profoundly perturb the biochemical cycles of the neighbor soil body.2 emission pulses. This reveals a common but long-ignored phenomenon in cold riparian zones, where progressive freeze-thaw can partition and thus shift the DOM compositions in stream water over melting time, and in turn profoundly perturb the biochemical cycles of the neighbor soil body., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Alzheimer's disease (AD, also known as dementia) has become a serious global health problem along with population aging, and neuroinflammation is the underlying cause of cognitive impairment in the brain. Nowadays, the development of multitarget anti-AD drugs is considered to be one effective approach. Imidazolylacetophenone oxime ethers or esters (IOEs) were multifunctional agents with neuroinflammation inhibition, metal chelation, antioxidant and neuroprotection properties against Alzheimer's disease. In this study, IOEs derivatives 1-8 were obtained by structural modifications of the oxime and imidazole groups, and the SARs showed that (Z)-oxime ether (derivative 2) had stronger anti-neuroinflammatory and neuroprotective ability than (E)-congener. Then, IOEs derivatives 9-30 were synthesized based on target-directed ligands and activity-based groups hybridization strategy. In vitro anti-AD activity screening revealed that some derivatives exhibited potentially multifunctional effects, among which derivative 28 exhibited the strongest inhibitory activity on NO production with EC 50 value of 0.49 μM, and had neuroprotective effects on 6-OHDA-induced cell damage and RSL3-induced ferroptosis. The anti-neuroinflammatory mechanism showed that 28 could inhibit the release of pro-inflammatory factors PGE 2 and TNF-α, down-regulate the expression of iNOS and COX-2 proteins, and promote the polarization of BV-2 cells from pro-inflammatory M1 phenotype to anti-inflammatory M2 phenotype. In addition, 28 can dose-dependently inhibit acetylcholinesterase (AChE) and Aβ 42 aggregation. Moreover, the selected nuclide [ 18 F]-labeled 28 was synthesized to explore its biodistribution by micro-PET/CT, of which 28 can penetrate the blood-brain barrier (BBB). These results shed light on the potential of 28 as a new multifunctional candidate for AD treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Microplastics (MPs) discharged from wastewater treatment plants (WWTPs) have emerged as serious pollutants in aquatic environments. Herein, a new magnetic filter (MFA) was prepared using an acidification-magnetization method with fly ash (FA) as the base material. The filter specifically targeted the removal of 1-μm polystyrene microspheres (PSMPs) because of the challenges they pose in filtration processes. The findings demonstrated that MFA filter exhibited superior PSMPs removal efficiency, with increases of 219%, 250%, and 288% compared to FA at flow rates of 1, 3, and 5 mL min -1 , respectively. Scanning electron microscopy and other characterizations provided insights into the removal mechanisms of PSMPs using the MFA filter, which combined electrostatic attraction, π-electron conjugation, hydrogen bonding, and complexation. Environmental variables, such as solution pH, ionic strength, and dissolved organic matter, were identified as considerable influences in the removal process of PSMPs. The practical application confirmed that the MFA filter considerably promoted the elimination of MPs from the secondary treatment effluent of WWTPs without having any toxic effects on freshwater fish. Thus, this study provides a new approach to the resource utilization of FA, which would prominently promote its application prospects in MPs immobilization and removal from wastewater effluent., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Plant and microbially derived carbon (C) are the two major sources of soil organic carbon (SOC), and their ratio impacts SOC composition, accumulation, stability, and turnover. The contributions of and the key factors defining the plant and microbial C in SOC with grassland patches are not well known. Here, we aim to address this issue by analyzing lignin phenols, amino sugars, glomalin-related soil proteins (GRSP), enzyme activities, particulate organic carbon (POC), and mineral-associated organic carbon (MAOC). Shrubby patches showed increased SOC and POC due to higher plant inputs, thereby stimulating plant-derived C (e.g., lignin phenol) accumulation. While degraded and exposed patches exhibited higher microbially derived C due to reduced plant input. After grassland degradation, POC content decreased faster than MAOC, and plant biomarkers (lignin phenols) declined faster than microbial biomarkers (amino sugars). As grassland degradation intensified, microbial necromass C and GRSP (gelling agents) increased their contribution to SOC formation. Grassland degradation stimulated the stabilization of microbially derived C in the form of MAOC. Further analyses revealed that microorganisms have a C and P co-limitation, stimulating the recycling of necromass, resulting in the proportion of microbial necromass C in the SOC remaining essentially stable with grassland degradation. Overall, with the grassland degradation, the relative proportion of the plant component decreases while than of the microbial component increases and existed in the form of MAOC. This is attributed to the physical protection of SOC by GRSP cementation. Therefore, different sources of SOC should be considered in evaluating SOC responses to grassland degradation, which has important implications for predicting dynamics in SOC under climate change and anthropogenic factors., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Excessive nonesterified fatty acids (NEFA) impair cellular metabolism and will induce fatty liver formation in dairy cows during the periparturient. Baicalin, an active flavonoid, has great potential efficacy in alleviating lipid accumulation and ameliorating the development of fatty liver disease. Nevertheless, its mechanism remains unclear. Here, the potential mechanism of baicalin on system levels was explored using network pharmacology and in vitro experiments. Firstly, the target of baicalin and fatty liver disease was predicted, and then the protein-protein interaction (PPI) network was constructed. In addition, the Kyoto Encyclopedia of Genes and Genomes (KEGG) (q-value) pathway enrichment is performed through the Database for Annotation, Visualization, and Integrated Discovery (DAVID) server. Finally, the results of the network analysis of the in vitro treatment of bovine hepatocytes by NEFA were confirmed. The results showed that 33 relevant targets of baicalin in the treatment of liver fatty were predicted by network pharmacology, and the top 20 relevant pathways were extracted by KEGG database. Baicalin treatment can reduce triglyceride (TAG) content and lipid droplet accumulation in NEFA-treated bovine hepatocytes, and the mechanism is related to inhibiting lipid synthesis and promoting lipid oxidation. The alleviating effect of baicalin on fatty liver may be related to the up-regulation of solute vector family member 4 (SLC2A4), Down-regulated AKT serine/threonine kinase 1 (AKT1), Peroxisome proliferator-activated receptor gamma (PPARG), Epidermal growth factor receptor (EGFR), tumor necrosis factor (TNF), Interleukin 6 (IL-6) were associated. These results suggested that baicalin may modulate key inflammatory markers, and lipogenesis processes to prevent fatty liver development in dairy cows., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The mechanism by which algal organic matter (AOM) affects the clogging of ceramic emitters remains unclear, which partially reduces the operational life of agricultural water distribution systems. This paper systematically investigated the clogging phenomenon of ceramic emitters under three different AOM concentrations. The results of irrigation tests revealed that the AOM significantly affects the degree of clogging of ceramic emitters, with higher AOM concentrations leading to faster flow reduction. By analyzing the original irrigation water and effluent and characterizing the clogged emitter surface, it was demonstrated that AOM was intercepted by the ceramic emitter, forming a dense biofilm. Infrared spectroscopy analysis revealed that polysaccharides and humic substances were the main clogging components. The clogging kinetics showed that as the AOM concentration increased, the clogging of the filter cake layer gradually become dominant. Further, the mechanism of interaction between AOM and silica ceramic emitters was explored from a microscopic perspective using molecular dynamics (MD) simulation with bovine serum albumin (BSA), sodium alginate (SA), and humic acid (HA) as model clogging substances in AOM. The simulation results indicated a strong interaction between AOM molecules and silica molecules dominated by electrostatic attraction, with the strength of the interaction as SA > HA > BSA. It was hypothesized that early clogging was mainly formed by polysaccharides and humic substances combining with silica molecules, while BSA was retained later by combining with organics on the clogging layer or through size exclusion. This study provides insights into bio-clogging in microporous ceramic emitters and may offer a theoretical basis for developing measures to control emitter clogging., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Soil contamination by microplastics (MPs) has emerged as a significant global concern. Although traditionally associated with crop production, contemporary understanding of soil health has expanded to include a broader range of factors, including animal safety, microbial diversity, ecological functions, and human health protection. This paradigm shifts underscores the imperative need for a comprehensive assessment of the effects of MPs on soil health. Through an investigation of various soil health indicators, this review endeavors to fill existing knowledge gaps, drawing insights from recent studies conducted between 2021 and 2024, to elucidate how MPs may disrupt soil ecosystems and compromise their crucial functions. This review provides a thorough analysis of the processes leading to MP contamination in soil environments and highlights film residues as major contributors to agricultural soils. MPs entering the soil detrimentally affect crop productivity by hindering growth and other physiological processes. Moreover, MPs hinder the survival, growth, and reproductive rates of the soil fauna, posing potential health risks. Additionally, a systematic evaluation of the impact of MPs on soil microbes and nutrient cycling highlights the diverse repercussions of MP contamination. Moreover, within soil-plant systems, MPs interact with other pollutants, resulting in combined pollution. For example, MPs contain oxygen-containing functional groups on their surfaces that form high-affinity hydrogen bonds with other pollutants, leading to prolonged persistence in the soil environment thereby increasing the risk to soil health. In conclusion, we succinctly summarize the current research challenges related to the mediating effects of MPs on soil health and suggest promising directions for future studies. Addressing these challenges and adopting interdisciplinary approaches will advance our understanding of the intricate interplay between MPs and soil ecosystems, thereby providing evidence-based strategies for mitigating their adverse effects., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The filamentation temperature-sensitive H (FtsH) metalloprotease participates in the chloroplast photosystem II (PSII) repair cycle, playing a crucial role in regulating leaf coloration. However, the evolutionary history and biological function of the FtsH family in albino tea plants are still unknown. In this study, 35 CsFtsH members, including 7 CsFtsH-like (CsFtsHi1-CsFtsHi7) proteins, mapping onto 11 chromosomes in 6 subgroups, were identified in the 'Shuchazao2' tea genome, and their exon/intron structure, domain characteristics, collinearity, protein interaction network, and secondary structure were comprehensively analyzed. Furthermore, real-time fluorescence quantitative PCR (RT-qPCR) analysis revealed that the expression levels of CsFtsH1/2/5/8 were significantly positively correlated with the leaf color of tea plants. The subcellular localization revealed that they were located in the chloroplast. The transgenic Arabidopsis has demonstrated that CsFtsH2 and CsFtsH5 could restore the chlorophyll content and chlorophyll fluorescence intensity in var1 and var2 mutants, respectively. Moreover, protein-protein interactions have confirmed that CsFtsH1 with CsFtsH5, and CsFtsH2 with CsFtsH8 could form a hetero-comples and function in chloroplasts. In summary, this study aims to not only increase the understanding of the underlying molecular mechanisms of CsFtsH but also to provide a solid and detailed theoretical foundation for the breeding of albino tea plant varieties., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Chemical properties and molecular diversity of dissolved organic matter (DOM) in agricultural soils are important for soil carbon dynamics and chlorine activity. Yet the chlorine reactivity of soil DOM at the molecular level under agricultural management practices remains unidentified. Here, we investigated the chlorine reactivity of soil DOM under long-term straw return and the molecular activities and transformations during chlorination. The 9-year straw return enhanced the chlorine reactivity of soil DOM, leading to increases in the production of traditional disinfection byproducts (DBPs) and decreases in the formation of emerging high molecular weight DBPs. C 17 H n O m Cl 1 - 2 and C 22 H n N m O z (0.41) ratios under straw return. The molecular transformation ability and inactive molecules of soil DOM under long-term straw return were reduced after chlorination, resulting in increased DOM instability. Chlorination led to a shift in the thermodynamic processes of soil DOM molecules from thermodynamically limited to thermodynamically favorable processes, and lignin-like compounds displayed higher potentials for transformation into protein/amino sugar-like compounds. C wa (1.47) and O/C wa (0.41) ratios under straw return. The molecular transformation ability and inactive molecules of soil DOM under long-term straw return were reduced after chlorination, resulting in increased DOM instability. Chlorination led to a shift in the thermodynamic processes of soil DOM molecules from thermodynamically limited to thermodynamically favorable processes, and lignin-like compounds displayed higher potentials for transformation into protein/amino sugar-like compounds. C 19 was identified as a sensitive formula for tracing chlorine reactivity under straw return, and a network illustrating the generation of DBPs from C 26 O 6 was identified as a sensitive formula for tracing chlorine reactivity under straw return, and a network illustrating the generation of DBPs from C 19 H 26 O 6 was established. Overall, these results highlighted the strong chlorine reactivity of soil DOM under long-term straw return., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

By analyzing the force-electric properties of rock-filled concrete under uniaxial compression, the precursor information and characteristics of deformation and failure of rock-filled concrete can be mastered, and the reliability and safety of rock-filled concrete structures at an early age can be ensured. This study investigated four variations of model rock-filled concrete, each with a distinct rock-filled ratio. Using the two-electrode alternating current test method, we analyzed the electrical resistivity properties of rock-filled concrete under uniaxial compression at various curing ages (1 d, 3 d, 7 d, 14 d, and 28 d). Additionally, the microscopic pore structure was examined using low-field nuclear magnetic resonance technology. The results showed that with increasing curing age or rock-filled ratio, the compressive strength and electrical resistivity of rock-filled concrete showed a nonlinear growth trend. In contrast, the porosity showed a nonlinear decrease, with the internal pore structure gradually becoming more refined. A mathematical model was established to describe the electrical resistivity of rock-filled concrete at various curing ages and rock-filled ratios. During uniaxial compression, the electrical resistivity of rock-filled concrete followed a pattern of rapid decline, slow decline, stable, and slow increase with strain. These phases corresponded to the development of internal pores and cracks and changes in the crack resistance performance of the rockfill skeleton in the concrete. Moreover, a mathematical equation was formulated to elucidate the relationship among the damage variable, the rock-filled ratio, and the electrical resistivity of model rock-filled concrete, thereby enabling the prediction of the extent of damage to the model rock-filled concrete under stress conditions., Competing Interests: Declaration of competing interest We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of the manuscript entitled “Force-electric characteristics of model rock-filled concrete under uniaxial compression with different curing ages and rock-filled ratios” (STOTEN-D-24-26477)., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Selecting appropriate reference genes is crucial for ensuring the accuracy and reliability of gene expression study using reverse transcription-quantitative PCR (RT-qPCR). To screen the optimal reference genes for analyzing gene expression in different tissues of the vector leafhopper Psammotettix striatus which causes extensive damage to a wide range of crops by vectoring multiple plant pathogenic microorganisms, the transcriptome data from Malpighian tubules (MTs) of P. striatus were mined. Twenty alternative candidate reference genes were initially selected for screening, among which seven genes with diverse Gene Ontology (GO) annotations were choosed as candidate reference genes, i.e., ribosomal protein L7A (RPL7A), ribosomal protein S28 (RPS28), ribosomal protein L22 (RPL22), ribosomal protein LP2 (RPLP2), H3 histone family 3A (H3F3A), elongation factor 1γ (EF-1γ), and elongation factor 1α (EF-1α). Gene expression levels in different tissues of P. striatus adults were examined using RT-qPCR, and their expression stability was analyzed using multiple reference gene screening software. This study revealed EF-1α as the most abundantly expressed gene, while RPL22 exhibited the lowest expression levels. EF-1α showed the most stable expression, whereas RPS28 showed the least stability. Various software tools confirmed EF-1α as the most stable single reference gene, and EF-1α and RPLP2 an optimal combination. This study provides a foundation for future investigation of the transmission of pathogenic microorganisms mediated by the vector leafhoppers, the function of the MTs, the biosynthesis of brochosomes, the coevolutionary processes and nutritional interactions of symbionts and host insects, and the gene expression study of other sap-sucking insects., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

In this study, the differences in the modification effects and related mechanisms of different times (20 and 40 min) of autoclaved heat (AH) treatment and different doses (2 and 4 kGy) of electron beam irradiation (EBI) in different sequences of effects on acorn starch were investigated. The results showed that both AH and EBI reduced the amylose content (22.70 to 19.59%) and enthalpy (10.28 to 1.84%) of starch but increased the resistant starch content (53.69 to 64.11%). AH treatment made the crystalline regions of the residual starch granules denser, which was resistant to the action of amylase enzymes. EBI degraded the long chain of starch, which increased the solubility. Notably, EBI pretreatment improves the reactive sites by inducing depolymerization and disorder in starch internal structure, thus increasing the modification extent of AH-modified starch, forming starch with lower viscosity, better hydration, and digestibility resistance, therefore being used as an ingredient for functional foods., Competing Interests: Declaration of competing interest The authors declare no financial or other conflicts of interest in this work, the data of this work has not been published elsewhere., (Copyright © 2023. Published by Elsevier Ltd.)

Recent studies have indicated a good potential for using solar-induced chlorophyll fluorescence (SIF) to estimate photosynthetic CO 2 assimilation. SIF can be emitted by both Photosystem I (PSI) and Photosystem II (PSII), but it is the SIF signals from PSII which are related to photosynthetic carbon fixation. However, since top-of-canopy SIF observations (SIF TOC ) always contain contributions from both photosystems, to mechanistically estimate gross primary productivity (GPP) from SIF, it is essential to extract PSII SIF from SIF TOC . Based on the differences in the relative contribution of PSII across different wavelengths, we propose a practical approach for extracting PSII contribution to SIF TOC at the near-infrared (NIR) band (f PSII&#95;760 ) using measurements of SIF TOC in the red and NIR spectral regions. A leaf-scale concurrent instrument was developed to assess the response of f PSII&#95;760 under varying environments. For winter-wheat leaves, as light intensity increased from 0 to 400 μmol m -2  s -1 , f PSII&#95;760 rose from 0.6 to 0.8; with further increase in light intensity to 1800 μmol m -2  s -1 , f PSII&#95;760 consistently decreased to 0.65. There was a slight decreasing trend in f PSII&#95;760 with rising temperatures, with values dropping from 0.65 at 15 °C to 0.61 at 40 °C. We found that variations in f PSII&#95;760 are due to changes in the fluorescence yield of PSII, with the two having a positively proportional relationship. We also estimated canopy-scale f PSII&#95;760 for a winter-wheat study site: f PSII&#95;760 varied from 0.61 to 0.83, with a mean value of 0.78 during the peak growing season. A comparison with eddy covariance-derived GPP reveals that GPP estimated with dynamic f PSII&#95;760 was more accurate than that calculated using fixed f PSII&#95;760 , with R 2 increasing from 0.6 to 0.84. This study contributes to a deeper understanding of the link between SIF and photosynthetic CO 2 assimilation, paving the way for more effective use of SIF to estimate GPP., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Microbial necromass carbon (MNC) contributes significantly to the formation of soil organic carbon (SOC). However, the microbial carbon sequestration effect of biochar is often underestimated and influenced by nutrient availability. The mechanisms associated with the formation and stabilization of MNC remain unclear, especially under the combined application of biochar and nitrogen (N) fertilizer. Thus, in a long-term field experiment (11 years) based on biochar application, we utilized bacterial 16S rRNA gene sequencing, fungal ITS amplicon sequencing, metagenomics, and microbial biomarkers to examine the interactions between MNC accumulation and microbial metabolic strategies under combined treatment with biochar and N fertilizer. We aimed to identify the critical microbial modules and species involved, and to analyze the sites where MNC was immobilized from various components. Biochar application increased the MNC content by 13.9 %. Among the MNC components, fungal necromass contributed more to MNC, but bacteria were more readily enriched after biochar application. The microbial life-history strategies that affected MNC formation under the application of various amounts biochar were linked to the N application level. Under N added at 226.5 kg ha -1 , communities such as Actinobacteria and Bacteroidetes with high-growth yield strategies were prevalent and contributed to MNC production. By contrast, under N added at 113.25 kg ha -1 with high biochar application, Proteobacteria with strong resource acquisition strategies were dominant and MNC accumulation was lower. The mineral-associated organic carbon pool was rapidly saturated with the addition of biochar, so the contribution of fungal necromass carbon may have been reduced by reutilization, thereby resulting in the more rapid preservation of bacterial necromass carbon in the particulate organic carbon pool. Overall, our findings indicate that microbial life history traits are crucial for linking microbial metabolic processes to the accumulation and stabilization of MNC, thereby highlighting the their importance for SOC accumulation in farmland soils, and the need to tailor appropriate biochar and N fertilizer application strategies for agricultural soils., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

In the context of global warming and low water and fertilizer utilization efficiency in vineyards, identifying the driving factors of global warming potential (GWP) and proper irrigation and fertilization management strategies are crucial for high grape yields and emission reduction. In this experiment, drip fertigation technology was used, including three irrigation levels (W3 (100% M, where M is the irrigation quota), W2 (75% M) and W1 (50% M)) and four fertilization levels (F3 (648 kg hm -2 ), F2 (486 kg hm -2 ), F1 (324 kg hm -2 ) and F0 (0 kg hm -2 )). Traditional furrow irrigation and fertilization (CG) and rainfed (CK) treatments were used as control treatments. The results indicated that under the drip fertigation system, fertilization significantly increased the grape leaf chlorophyll relative content (SPAD) and leaf area index (LAI) within a fertilizer application of 0-486 kg hm -2 . Irrigation primarily had a direct positive effect on the water-filled pore space (WFPS) in the 0-60 cm soil layer, and the residual soil nutrient content was mainly affected by fertilization. The vital stage for reducing greenhouse gas emissions was the fruit-inflating and fruit-rendering stages. The CG treatment not only failed to ensure high grape yield but also adversely affected the soil environment and the reduction of greenhouse gas emissions in the vineyard. Fertilization had a direct positive effect on the grape SPAD, LAI, yield, and soil residual nutrient content. GWP was primarily directly driven by SPAD, WFPS, and soil residual nutrient content, while grape yield was primarily directly driven by fertilization and SPAD. In conclusion, the W2F2 treatment (25 % reduced irrigation and 486 kg hm -2 of fertilization) of drip fertigation in the vineyard was the preferred irrigation and fertilizer management strategy for maintaining good vine vigor and balancing grape yield and environmental benefits., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Selenium (Se)-rich farmland is a valuable and nonrenewable resource for addressing the global challenge of Se deficiency. However, frequent warnings of heavy metal pollution have threatened the safety and legitimacy of Se-rich functional agriculture, eventually damaged public health security. Definitive and judgmental quantitative studies on this hazardous phenomenon are still missing. Relevant reviews published in the past have summarized textual descriptions of the problem, lacking the support of the necessary statistical analysis of the data. Based on the collected publications, the present study evaluated and analyzed the sources, risks and impacts of heavy metal pollution in Se-rich farmland. Concentrations of cadmium (Cd), arsenic, lead and zinc in Se-rich farmland were significantly higher than those in non-Se-rich farmland, especially Cd. Pollution source analyses indicated that Se enrichment and heavy metal pollution occurred simultaneously in farmland, related to Se-heavy metal homology in rocks. According to environmental risk assessment, both serious Cd pollution and the narrow Se concentration range of safety utilization limited the availability of Se-rich farmland. Pollution impact predictions showed that the pollution in Se-rich farmland would result in serious human health risks to consumers and economic losses of 4000 yuan/hm 2 on production side. Tackling Cd pollution was anticipated to recover economic losses (81 %) while lowering the carcinogenic (60 %) and non-carcinogenic (10 %) health risks. Our study also provided recommendations to address heavy metal pollution in Se-rich farmland. The two criteria should be followed by pollution control strategies applied to Se-rich functional agriculture including (i) not affecting the original Se enrichment in plant and (ii) not being interfered by Se in soil-plant systems. This will provide valuable information for Se-rich functional agriculture and public health security., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Free radical therapy, based on the sulfate radical derived from peroxymonosulfate, has recently been explored as a potential cancer treatment. However, while it is promising, its successful application is restricted by several limitations including the uncontrollable generation of free radicals and the instability in aqueous medium. Herein, we prepared LCP nanoparticles by using PMS as a core, the Co-coordination polymer (Co-CP) as a coating layer, and lactobionic acid as a targeting ligand for hepatoma carcinoma cells. LCP could be activated by cobalt ions released from Co-CP, and successfully induced apoptosis and ferroptosis via the inhibition of glutathione peroxidase 4 and caused the accumulation of lipid peroxidation to enhance the efficacy of free radical therapy.

Microbial production offers a sustainable route to plant- and chemical-based manufacturing. 2-Phenylethanol (2-PE) has been widely used in foods, flavors, and pharmaceuticals. Herein, de novo biosynthesis of 2-PE was achieved for the first time by rewiring the non-native producer Rhodotorula toruloides . Our results showed that the generation of phenylalanine (Phe) and the recycling of the amino group could be bypassed since the Ehrlich pathway outperformed the phenylacetaldehyde-dependent one in de novo biosynthesis of 2-PE when the adenosine 5'-triphosphate (ATP) citrate synthase (ACL) was inactivated. The 2-PE titer was enhanced to 151.7 mg/L in a shake flask by alleviating feedback inhibition, enhancing precursor availability and cofactor balance. Finally, the production of 2-PE was elevated to 1.06 g/L with a yield of 8.5 mg/g glucose and productivity of 8 mg/L/h in a 3 L bioreactor. Our results should shed light on the microbial production of other aromatic derivatives with R. toruloides .

Background: Biofilm plays a crucial role in Pseudomonas syringae pv. tomato (Pst) infection. We identified (1R,4S)-(-)-fenchone (FCH) as the most potent antibiofilm agent against Pst among 39 essential oil compounds. Subsequently, we synthesized a series of FCH oxime ester and acylhydrazine derivatives to explore more potent derivatives., Results: II3 was screened out as the most potent derivative, exhibiting a minimal biofilm inhibitory concentration of 60 μg mL -1 and a lowest concentration with maximal biofilm inhibition (LCMBI) of 200 μg mL -1 , lower than those of FCH (80 and 500 μg mL -1 , respectively). II3 and FCH showed minimum inhibitory concentration values >1000 μg mL -1 and similar maximal biofilm inhibition extents of 48.7% and 49.5% at their respective LCMBIs, respectively. Meanwhile, neither of them influenced cell viability or the activity of metabolic enzymes at their respective LCMBIs. II3 at its LCMBI significantly reduced biofilm thickness, extracellular polysaccharide content, and pectinase and cellulase production indices. In vivo assay results indicated that II3 could preventatively reduce the bacterial contents in tomato leaves at its LCMBI, and when combined with kasugamycin (KSG) (10 μg mL -1 ), II3 achieved the same level of bacterial reduction as the sole application of KSG (70 μg mL -1 ), thereby reducing the required dosage of KSG. Mechanistic studies demonstrated that II3 can down-regulate biofilm-related genes and inhibit PsyR/PsyI quorum sensing system, which differs from the bactericidal mechanisms., Conclusion: These results underscore the potential of II3 as an antibiofilm agent for the control of Pst or FCH as a promising natural candidate for future in-depth optimization. © 2024 Society of Chemical Industry., (© 2024 Society of Chemical Industry.)

The lactation-pregnancy overlap in the industrialized 49-day breeding model increases nutritional demands for lactating-pregnant rabbit does. This study examined the effects of folic acid (FA) on the production performance and intestinal microflora of does with different litter size (LS, or number of kits). A total of 144 third-parity Hyplus does, aged 11 months and weighing approximately 5.00 ± 0.07 kg, were divided four treatment groups: control group (basal diet) and FA groups (basal diet + 15, 30, and 45 mg/kg FA). Does with LS of 5, 7, and 9 were slected after weaning. Our findings revealed that 1) Increased FA supplementation initially increased and then decreased the pre-lactation body weight of 21-day-old kits and prolactin (PRL) level of does, while showing an inverse trend for estrogen (E) level of does. Increased LS significantly (P < 0.05) correlated with reduced milk yield of does. There was a significant (P < 0.05) interaction between FA and LS affecting PRL, E, growth hormone (GH) levels of does, and the pre-lactation body weight of 21-day-old kits. 2) Increased FA supplementation initially increased and then decreased the post-lactation body weight of 21-day-old and 35-day-old kits, elevated plasma folic acid (PFA) level of does, and significantly (P < 0.05) impacted the pregnancy rate of does. Increased LS was associated with reduced post-lactation body weight in 21-day-old and 35-day-old kits (P < 0.05). There was a significant (P < 0.05) interaction between FA and LS affecting plasma insulin-like growth factor-1 (IGF-1), homocysteine (HCY), PFA levels of does, post-lactation body weight of 21-day-old kits, and weaning body weight of 35-day-old kits. 3) FA supplementation promoted the growth of Ruminococcaceae and Rikenella&#95;RC9&#95;gut&#95;group in feces of does, indicating enhanced anti-inflammatory capabilities and crude fiber (CF) breakdown. In summary, FA supplementation improved conception rates, regulated lactation-related hormone synthesis and secretion, increased body weight of 21-day-old kits, and enhanced intestinal anti-inflammatory functions., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

This study conducted a systematic analysis to explore natural DNA topoisomerase I (topo I) inhibitors from Forsythiae Fructus ( FF ). Crude extract of FF exhibited notable toxic and anti-proliferative effects on A549 cells. A total of 36 components were identified using bioaffinity ultrafiltration UPLC-ESI-MS/MS. Pinoresinol, 1,8-dihydrox-yanthraquinone, quercetin, and lariciresinol were screened as topo I inhibitors. Their ESI fragmentation patterns were analysed. An obvious repair effect on damaged DNA strands was observed by topo I inhibitory binding assay. Moreover, a common feature-based pharmacophore model was constructed and another 7 topo I inhibitors were screened. Molecular docking indicated that hydrogen bond, π-anion, and π-alkyl interaction were major interactions. Molecular dynamics simulation revealed important residues determining the binding of amentoflavone, forsythoside B and topo I. The results improved current understanding of natural topo I inhibitors from FF . Moreover, the combination of multi-disciplinary approaches provided a new tool to investigate natural antitumor products.

Recently, research and development of novel pesticides from natural plant products have received much attention. To accelerate the application of sophocarpine as the agrochemical candidate, a series of novel sophocarpine-based isoxazoline derivatives were prepared by the 1,3-dipolar [2 + 3] cycloaddition reaction of sophocarpine with different chloroximes. Their structures were well characterized by high-resolution mass spectra, infrared spectra, and proton/carbon-13 nuclear magnetic resonance spectra. Eight steric configurations of compounds 5a , 5e' , 5f , 5g , 5h , 5i , 5r , and 5u' were further determined by X-ray single-crystallography. Against Aphis citricola Van der Goot, compounds 5n (LD 50 : 0.032 μg/nymph) and 5o (LD 50 : 0.024 μg/nymph) exhibited greater than 3.7- and 4.9-fold potent aphicidal activity compared to sophocarpine (LD 50 : 0.118 μg/nymph). Against Tetranychus cinnabarinus Boisduval, derivative 5g displayed the most promising acaricidal activity with the LC 50 value of 0.247 mg/mL, which was 14.2-fold that of sophocarpine. Compounds 5d and 5g also exhibited good control efficacy against T. cinnabarinus . Scanning electron microscopy images indicated that compound 5g can destroy the mite cuticle layer. These results will provide the foundation for the structural modification and use of sophocarpine derivatives as agrochemicals in the future.

Intramuscular fat (IMF) content is an economic trait in beef cattle that improves the meat quality. Studies have highlighted the correlation between long noncoding RNAs (lncRNAs) and IMF development. In this study, lncBNIP3 knockdown promoted bovine intramuscular preadipocyte differentiation. RNA-seq analysis of intramuscular preadipocytes with lncBNIP3 knockdown identified 230 differentially expressed genes. The PI3K-Akt and PPAR signaling pathways were enriched. lncBNIP3 interference promoted mRNA and protein expression of key genes in PI3K-Akt signaling pathway. LncBNIP3 interference reversed the effects of an AKT-inhibitor MK-2206 on Akt protein expression and lipid droplet accumulation, promoted mRNA and protein expression of essential genes in the PPAR signaling pathway, and ameliorated the inhibitory effects of a PPARg antagonist GW9662 on lipid accumulation. Therefore, lncBNIP3 inhibition of bovine intramuscular preadipocyte differentiation is likely mediated via the PI3K-Akt and PPAR signaling pathways. This study identified a valuable lncRNA with functional roles in IMF accumulation and revealed new strategies to improve beef quality.

Azo-incorporating was reported to be an effective strategy for increasing SDH inhibitory activity but for poor in vivo control effects. Herein, the azo-incorporated compounds were structurally optimized to retain a preferential conformation by replacing the azo bond with their bioisosteres. Interestingly, the 1,2,4-oxadiazole compound D2 displayed a broad fungicidal spectrum as well as fluxapyroxad. More excitedly, compound D2 showed excellent antifungal activities against rice sheath blight disease both in vitro (EC 50 = 0.001 μg/mL) and in vivo (EC 50 = 1.08 μg/mL, EC 95 = 4.67 μg/mL). In addition, an extra π-π interaction was found between the 1,2,4-oxadiazole ring of compound D2 and the phenyl ring of residue D&#95;Y586, which might interpret the enhanced potency of compound D2 against Rhizoctonia solani . Further structural optimizations of the 1,2,4-oxadiazole compounds gave several analogues that made a breakthrough in controlling rice blast disease. These 1,2,4-oxadiazole compounds, derived from azobenzene derivatives, could be antifungal leads especially against R. solani and Magnaporthe grisea , exemplifying an interesting mode of pesticide discovery and providing theoretical guidance for innovation of the SDHI fungicide.

The ion interfacial transport driven by ion-surface interactions in calcareous soil has a profound impact on the nutrient storage and environmental buffer capacity of the main agricultural soils in dry and semi-arid areas. The roles that carbonate plays in preserving the soil's inorganic carbon pool and soil structure stability have been widely investigated, but its significance in the aforementioned microscopic processes, especially the influence of carbonate on the interfacial reaction kinetics of nutrient elements, is yet to be determined. In this study, potassium (K) was used as an indicator ion to investigate its affinity in carbonate-removed (CREM) and carbonate-reserved (CRES) calcareous soil using the general theory of ion diffusion in an external electric field. We discovered that (1) at a given initial K concentration, the carbonate in CRES soil retards the adsorption rate and diminishes the adsorption amount of K in calcareous soil, reducing the interfacial transport properties of nutrient ions at the solid-liquid interface of calcareous soils compared with CREM soil; and (2) this weakening of the interfacial transport effect on nutrient K originates from the soil carbonate, which prefers to weaken the electrostatic interaction intensity between K and the calcareous soil surface. Furthermore, this is due to the carbonate shielding effect on the surface adsorption sites of other soil components and the competitive relationship between K + and cations released by carbonate dissolution. The influence of carbonate on the nutrient ion transport at the solid-liquid interface of calcareous soil has been investigated by soil electrochemistry theory-based ion adsorption kinetics, and the links between kinetic features and ion-surface binding energy have been clarified., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

The differentiation and maturation of osteoblasts are essential for bone formation. Zn 2+ plays a crucial role in cell differentiation and is involved in osteogenic differentiation. The concentration and distribution of Zn 2+ in the nucleus and cytoplasm indicate the differentiation states of osteoblasts. However, there is an absence of a real-time method for monitoring the dynamic fluctuations of endogenous Zn 2+ within the nucleus. Here, a novel Zn 2+ fluorescent probe ( NTAD-N1 ) with nuclear membrane permeability was designed and developed, allowing for distribution throughout the entire cell, including the nucleus. The NTAD-N1 probe successfully showed the dynamic distribution and concentration changes of Zn 2+ in the nucleus and cytoplasm of preosteoblast MC3T3-E1 during the 21-day differentiation period. The results showed that free Zn 2+ increased significantly during differentiation of osteoblasts (2-21 days). Importantly, after 4 days of differentiation, osteoblasts are mainly distributed in the nucleus, which is confirmed by metallothionein expression. Subsequently, the level of free Zn 2+ in the cytoplasm remained at a high level, which promoted the increase in alkaline phosphatase activity and inhibited the activity of cis-aconitase in the tricarboxylic acid cycle, resulting in the accumulation of citric acid. This series of events promotes the formation of mineralized nodules. In the process of osteoblast differentiation, the detection time of Zn 2+ (≤7 days) is ahead of the late marker of alkaline phosphatase (14 days) and mineralized nodules (14-21 days). This indicates that Zn 2+ can be used as a biomarker and an intervention point for early differentiation of osteoblasts.

Protein-nanoparticle interactions play a crucial role in both biomedical applications and the biosafety assessment of nanomaterials. Here, we found that nanobodies can induce citrate-capped gold nanoparticles (AuNPs) to aggregate into large clusters. Subsequently, we explored the mechanism behind this aggregation and proposed the "gold nucleation mechanism" to explain this phenomenon. Building on this observation, we developed a one-step label-free colorimetric method based on nanobody-induced AuNP aggregation. When nanobodies bind to target bacteria, spatial hindrance occurs, preventing further AuNPs aggregation. This alteration in surface plasmon resonance properties results in visible color changes. As an example, we present a simple and sensitive "mix-and-read" chromogenic immunosensor for Cronobacter sakazakii ( C. sakazakii ). The experiment can be completed within 20 min, with a visual detection limit of 10 3 CFU/mL and a quantitative detection limit of 136 CFU/mL. Importantly, our method exhibits no cross-reactivity with other bacterial species. This strategy harnesses the excellent properties of nanobodies and the optical characteristics of AuNPs for direct and rapid detection of foodborne pathogen.

Mycotoxin-producing fungi are widespread and their adverse effects on mammals have been investigated; however, their impacts on soil invertebrates are not fully understood. Folsomia candida is a model soil arthropod that represents an important part of the soil invertebrate community. This study investigated the consequences of F. candida grazing on mycotoxin-producing fungi Fusarium verticillioides , F. graminearum , Aspergillus ochraceus , and A. nidulans . Consuming mycotoxin-producing fungi affected the body size and reproductive ability of F. candida , and altered the gut bacterial composition, with decreased Proteobacteria and increased Actinobacteria ( Microbacterium ) abundances. Notably, the abundance of foodborne fungi can be detected. Furthermore, certain bacteria isolated from F. candida 's gut inhibited the growth of corresponding mycotoxin-producing fungi. The gut bacteria that inhibited mycotoxin-producing fungi growth in Aspergillus groups were also associated with poor fitness parameters and larger disruption in gut microbiota. Importantly, switching back to yeast diets reversed both the fitness parameters and gut bacterial composition. Together, our study demonstrated that grazing of mycotoxin-producing fungi by F. candida resulted in reduced physiological parameters and disturbed the gut bacterial community, and those changes can be restored by switching back to yeast diets, which indicates a strong resilience of springtails to mycotoxin-producing fungi., Importance: Mycotoxin-producing fungi are widespread in nature and raise concerns for human and livestock health. Although they share the same ecosystem, interactions between mycotoxin-producing fungi and soil arthropods are not well understood. In this study, we report an unexpected finding that the soil arthropod Folsomia candida is rather tolerant to these mycotoxin-producing fungi. F. candida can survive solely on mycotoxin-producing fungi as a food source with reduced physiological parameters. Moreover, the gut microbial community is disturbed by mycotoxin-producing fungi, and some of the bacteria isolated from F. candida 's gut can inhibit the growth of corresponding fungi. Notably, the altered physiological parameters and gut microbiota are restored when a normal diet is reintroduced, suggesting F. candida 's resilience to mycotoxin-producing fungi. These findings clarify the impact of toxin-producing diets on F. candida , shedding light on how organisms can build resilience to environmental stimuli., Competing Interests: The authors declare no conflict of interest.

Spread of antibiotic resistance genes (ARGs) in aquatic ecosystems poses a significant global challenge to public health. The potential effects of water temperature perturbation induced by specific water environment changes on ARGs transmission are still unclear. The conjugate transfer of plasmid-mediated ARGs under water temperature perturbation was investigated in this study. The conjugate transfer frequency (CTF) was only 7.16 × 10 -7 at a constant water temperature of 5 °C, and it reached 2.18 × 10 -5 at 30 °C. Interestingly, compared to the constant 5 °C, the water temperature perturbations (cooling and warming models between 5-30 °C) significantly promoted the CTF. Intracellular reactive oxygen species was a dominant factor, which not only directly affected the CTF of ARGs, but also functioned indirectly via influencing the cell membrane permeability and cell adhesion. Compared to the constant 5 °C, water temperature perturbations significantly elevated the gene expression associated with intercellular contact, cell membrane permeability, oxidative stress responses, and energy driven force for CTF. Furthermore, based on the mathematical model predictions, the stabilization times of acquiring plasmid maintenance were shortened to 184 h and 190 h under cooling and warming model, respectively, thus the water temperature perturbations promoted the ARGs transmission in natural conditions compared with the constant low temperature conditions., Competing Interests: Declaration of Competing Interest There is no conflict of interest between the authors., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Although extensive research has been conducted on the environmental impact of microplastics (MPs), their effects on microorganisms during the composting process and on the compost-soil system remain unclear. Our research investigates the microbial response to polylactic acid microplastics (PLAMPs) during aerobic composting and examines how compost enriched with PLAMPs affects plants. Our findings reveal that PLAMPs play a dual role in the composting process, influencing microorganisms differently depending on the composting phase. PLAMPs reduce the relative abundance of sensitive bacterial ASVs, specifically those belonging to Limnochordaceae and Enterobacteriaceae, during composting, while increasing the relative abundance of ASVs belonging to Steroidobacteriaceae and Bacillaceae. The impact of PLAMPs on microbial community assembly and niche width was found to be phase-dependent. In the stabilization phase (S5), the presence of PLAMPs caused a shift in the core microbial network from bacterial dominance to fungal dominance, accompanied by heightened microbial antagonism. Additionally, these intricate microbial interactions can be transferred to the soil ecosystem. Our study indicates that composting, as a method of managing PLAMPs, is also influenced by PLAMPs. This influence is transferred to the soil through the use of compost, resulting in severe oxidative stress in plants. Our research is pivotal for devising future strategies for PLAMPs management and predicting the subsequent changes in compost quality and environmental equilibrium., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Myocarditis is characterized as local or diffuse inflammatory lesions in the myocardium, primarily caused by viruses and other infections. It is a common cause of sudden cardiac death and dilated cardiomyopathy. In recent years, the global prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the widespread vaccination have coincided with a notable increase in the number of reported cases of myocarditis. In light of the potential threat that myocarditis poses to global public health, numerous studies have sought to elucidate the pathogenesis of this condition. However, despite these efforts, effective treatment strategies remain elusive. To collate the current research advances in myocarditis, and thereby provide possible directions for further research, this review summarizes the mechanisms involved in viral invasion of the organism and primarily focuses on how viruses trigger excessive inflammatory responses and in result in different types of cell death. Furthermore, this article outlines existing therapeutic approaches and potential therapeutic targets for the acute phase of myocarditis. In particular, immunomodulatory treatments are emphasized and suggested as the most extensively studied and clinically promising therapeutic options., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

E3 ligases are essential for ubiquitination and play a role in regulating various aspects of eukaryotic life. Ariadne (ARI) proteins, a subfamily of RBR (RING-between-RING) proteins, have been recognized as a new class of RING-finger E3 ligases. Recent research has shed light on their potential involvement in plants' responses to abiotic stress. However, comprehensive studies on ARI genes in apple (Malus domestica) are still lacking. This study identified ten MdARI genes in the apple genome, and examined intraspecific and interspecific collinearity to explore the evolutionary relationships of ARI family members. Phylogenetic analyses classified MdARIs into two subfamilies (A and B), and by integrating gene structure, conserved motifs, and sequence comparison results, subfamily B was further divided into two subgroups (I and II). Tissue expression analyses revealed varied expression patterns of MdARI genes in different tissues, and subcellular localization showed that MdARI1-1, MdARI1-2, and MdARI9-1 were located in the nucleus, while the other seven MdARIs were distributed throughout the cell. Analyses of promoter cis-elements and expression patterns under cold, salt, and drought treatments indicated the involvement of MdARIs in abiotic stress responses. Several proteins crucial to the plant stress response were predicted to be potential MdARIs-interacting proteins based on the protein interaction network. Additionally, the interaction between UBC11 (E2) and MdARI7-2 was confirmed by a yeast two-hybrid (Y2H) experiment, suggesting that MdARI7-2 may function as an E3. These findings will greatly benefit future research on the role and mechanisms of ARI proteins in apple stress response., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Biochar application emerges as a valuable soil management strategy for enhancing crop yield; however, the mechanisms underlying the relationships between soil and plants remain unclear after biochar application. In this study, soil pore characteristics and maize yield were assessed in a five-year biochar-application experiment on the Loess Plateau of China, including four treatments: Control (no biochar), low-dose biochar application (LB, 3 t ha -1 ), moderate-dose biochar application (MB, 6 t ha -1 ), and high-dose biochar application (HB, 9 t ha -1 ). Root growth traits were evaluated by cultivating maize in intact soil cores collected from field conditions using X-ray computed tomography. Our findings indicate that, compared to the Control, the HB treatment enhanced macroporosity (> 0.1 mm in diameter), porosity of 0.1-0.5 mm pores, and saturated water content, while reducing macropore connectivity and penetration resistance. However, biochar application treatments did not alter the water retention characteristics from field capacity to permanent wilting point or the plant-available water content (PAWC). Furthermore, the mean angle of primary and seminal roots as well as the length and surface area of entire roots increased in the HB treatment, showing a positive correlation with the porosity of 0.1-0.5 mm pores. The mean diameter of primary and seminal roots, leaf fresh and dry weights, and maize yield also increased in the HB treatment compared to the Control. Partial least squares path modeling analysis indicated that biochar application rates positively impacted on root growth and plant productivity through an indirect influence of soil pore size distribution, with 0.1-0.5 mm pores being particularly crucial for facilitating deeper root penetration and root elongation. These findings demonstrate that biochar application primarily augmented 0.1-0.5 mm pores, rather than affecting smaller pores capable of retaining plant-available water or larger macropores, enhancing deeper rooting and root elongation, thus improving plant productivity and crop yield., (Copyright © 2024. Published by Elsevier B.V.)

Sea buckthorn ( Hippophaë rhamnoides L.) known as the deciduous shrub has been reported to have effects of antioxidant, anti-inflammatory, and immunomodulatory activities. Tropomyosin (TM) induced a regulatory immune response associated with food allergy. In this study, a mouse model of food allergy sensitized to tropomyosin (TM) was established to assess the antiallergic properties of sea buckthorn flavonoid extract (SBF). SBF alleviated mice's allergic symptoms and exhibited a significant reduction in the levels of IgE and histamine. Meanwhile, SBF repaired the allergic Th2 cell overpolarization generated by TM, via downregulating the IL-4 production and upregulating IFN-γ production to restore the balance of Th1/Th2 cells. Furthermore, the 16S RNA analysis showed that SBF primarily restored the gut microbiota via increasing the abundance in Chitinophilidae and decreasing in Burkholderiaceae, Pneumatobacteriaceae , and Sphingomonadaceae . Gut metabolomes determined by liquid chromatography-mass spectrometry (LC-MS) suggested that TM upregulated PE (14:0/22:1(13Z)) and SBF decreased formimino-l-glutamic acid and urocanic acid levels. According to the KEGG pathway analysis, SBF treatment has been shown to modulate glycerophospholipid and histidine metabolism to improve allergic reactions. SBF holds great promise as a novel potential agent for the treatment of food allergies.

Background: Chinese jujube (Ziziphus jujuba Mill.), also called Chinese date, is one of the oldest and widely cultivated fruit trees with great economic values, which, at least, can be attributed to the melliferous flower with highly developed nectary that can secret huge amount of nectar in a rather tiny floral size. However, the morphological nature, metabolic products, developmental process, as well as molecular and regulatory mechanisms of jujube nectary remain largely unknown., Results: Here, we selected Z. jujuba 'Dongzao' as a system to address these questions. We uncovered that the jujube nectary is an annular or donut-shaped secretory protrusion that surrounds the base of the carpels, along with emerald and glistening hues, which can produce a bulk honey with many metabolic compounds (e.g. saccharides and flavonoids) that has a high nutritional value and benefit for human health. The development of jujube nectary is a dynamic process of earlier cell division followed by later cell expansion. We also identified putative genes associated with the nectary development and found that the CRABS CLAW (CRC) ortholog (ZjCRC) is the key to nectary development: the gene is highly expressed in nectary; ectopic expression of it in the Arabidopsis crc-1 mutant rescued the lost nectary (also the carpel and silique defects). We also demonstrated that a MADS-box transcription factor ZjAGAMOUS1 (ZjAG1) is required for the direct activation of ZjCRC expression., Conclusions: Taken together, our results not only provide a comprehensive portrait of the jujube nectary, but also pave the way to effective utilization of jujube and other woody crops., (© 2024. The Author(s).)

Hexokinases (HXKs), which sense and catalyze cellular sugar, play a critical role in the growth and development of various plants, including wheat, a primary source of human calories frequently attacked by fungal pathogens. However, the evolutionary dynamics and functional diversification of HXKs in wheat, particularly their roles in plant defense, remain unclear. Here, we discovered that the wheat hexokinase gene family originated through multiple ancient gene duplications across different plant lineages and has undergone comprehensive, multidimensional functional specialization in gene expression, subcellular localization, enzyme activity, and regulation of plant defense responses. Gene expression analysis suggests that two-thirds of the TaHXK genes are responsive to fungal infection. Subcellular analysis reveals that while six TaHXKs are localized in mitochondria, three TaHXKs from different phylogenetic branches are sorted into other cellular compartments. Notably, biochemical analysis shows that TaHXKs in mitochondria differ in their glucose-catalyzing activity, with TaHXK5 and TaHXK3 exhibiting the highest and lowest enzyme activity, respectively. Consistently, transient expression analysis suggests that TaHXK5 induces various plant defense responses, while TaHXK3 is defective in activating some plant defense responses. Furthermore, inactivation of the glucokinase activity of TaHXK5 compromised its function in defense activation, suggesting that mitochondrial TaHXKs display functional divergence in both enzyme activity and defense-inducing activity that are intrinsically connected. Overall, our findings reveal that the multidimensional specialization events following the ancient duplication events may have shaped the functional diversity of HXKs in wheat, shedding light on their evolutionary dynamics and potentially contributing to the improvement of wheat defense., (© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)

Alcohol-related liver disease (ALD) encompasses a spectrum of hepatic disorders resulting from alcohol abuse, which constitutes the predominant etiology of morbidity and mortality associated with hepatic pathologies globally. Excessive alcohol consumption disrupts the integrity of the intestinal barrier and perturbs the balance of gut microbiota, thereby facilitating the progression of ALD. Ellagic acid (EA) has been extensively reported to be an effective intervention for alleviating liver symptoms. However, the target molecules of EA in improving ALD and its underlying mechanism remain elusive. First, our study indicates that EA ameliorated ALD through the hepatic circadian rhythm signaling by up-regulating neuronal PAS domain protein 2 (NPAS2). Furthermore, analysis of the intestinal microbiome showed that EA significantly enhanced the abundance of beneficial bacteria, which was positively correlated with NPAS2 expression and negatively correlated with liver injury. Finally, antibiotic treatment and fecal microbiota transplantation (FMT) experiments established a causal relationship between the reshaped microbiota and NPAS2 in the amelioration of ALD. In summary, our study demonstrates novel evidence that EA attenuated ALD by modulating the hepatic circadian rhythm signaling pathway via the gut microbiota-NPAS2 axis, providing valuable insights for EA and microbiome-targeted interventions against ALD.

Selenium (Se) and zinc (Zn) deficiencies have become serious global food security and public health problems. Biofortification through foliar fertilizer is a nonspecific, low-tech, and cost-effective strategy. Se and Zn have overlapping physiological roles and interacting relationships in plants. Mung bean is superior for Se enrichment and an excellent Zn carrier. However, the molecular mechanism underlying the interaction between Se and Zn in the mung bean remains unclear. Herein, Se and Zn accumulation, antioxidant activities, physiological determination, and transcriptomic analysis were performed under both Se and Zn treatments. Common essential roles of Se and Zn in mung bean were reflected by the comprehensively altered ten physiological indexes under both Se2 (24 g·ha -1 ) and Zn1 (1.2 kg·ha -1 ) treatments. Overlapping transcriptome changes and common DEGs in two compared groups revealed that the upregulated expression of sulfate transporters (SULTRs), phosphate transporters (PHTs), and Zinc-regulated/Iron-regulated-like protein (ZIP) family genes under Se and Zn treatments directly promoted both Se and Zn intakes. Furthermore, the altered Se/Sulfur, nitrogen, and carbohydrate metabolisms are closely interlinked with the uptake and assimilation of Se and Zn via the 20 key genes that we filtered through the protein-protein interaction (PPI) network analysis. Further analysis indicated that l-methionine γ-lyase (E 4.4.1.11) genes may play an important role in the transamination of selenomethionine and its derivatives; glutamine synthetase (GS), nitrate reductase (NR), and starch synthase (SS) genes may regulate the nitrogen assimilation and carbohydrate metabolism, which provide more carriers for Se and Zn; glutathione peroxidase (GPx), glutamate-cysteine ligase catalytic subunit (GCLC), and serine acetyltransferase (SAT) genes may accelerate the GSH-GSSH cycle and promote Se and Zn storages. This study provides new molecular insights into the comprehensive improvement of the nutritional quality of mung beans in Se and Zn biofortification productions.

Flesh firmness is a critical breeding trait that determines consumer selection, shelf life, and transportation. The genetic basis controlling firmness in apple (Malus × domestica Borkh.) remains to be fully elucidated. We aimed to decipher genetic variance for firmness at harvest and develop potential molecular markers for marker-assisted breeding. Maturity firmness for 439 F1 hybrids from a cross of "Cripps Pink" and "Fuji" was determined in 2016 and 2017. The phenotype segregated extensively, with a Gaussian distribution. In a combined bulked segregant analysis (BSA) and RNA-sequencing analysis, 84 differentially expressed genes were screened from the 10 quantitative trait loci regions. Interestingly, next-generation re-sequencing analysis revealed a Harbinger-like transposon element insertion upstream of the candidate gene PECTATE LYASE5 (MdPL5); the genotype was associated with flesh firmness at harvest. The presence of this transposon repressed MdPL5 expression and was closely linked to the extra-hard phenotype. MdPL5 was demonstrated to promote softening in apples and tomatoes. Subsequently, using the MdPL5 promoter as bait, MdNAC1-L was identified as a transcription activator that positively regulates ripening and softening in the developing fruit. We also demonstrated that MdNAC1-L could induce the up-regulation of MdPL5, MdPG1, and the ethylene-related genes MdACS1 and MdACO1. Our findings provide insight into TE-related genetic variation and the PL-mediated regulatory network for the firmness of apple fruit., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

Saline-alkali stress is a widely distributed abiotic stress that severely limits plant growth. γ-Aminobutyric acid (GABA) accumulates rapidly in plants under saline-alkali stress, but the underlying molecular mechanisms and associated regulatory networks remain unclear. Here, we report a MYB-like protein, I-box binding factor (SlMYBI), which positively regulates saline-alkali tolerance through induced GABA accumulation by directly modulating the glutamate decarboxylase (GAD) gene SlGAD1 in tomato (Solanum lycopersicum L.). Overexpression of SlGAD1 increased GABA levels and decreased reactive oxygen species accumulation under saline-alkali stress, while silencing of SlGAD1 further suggested that SlGAD1 plays an active role in GABA synthesis and saline-alkali tolerance of tomato. In addition, we found that SlMYBI activates SlGAD1 transcription. Both overexpression of SlMYBI and editing of SlMYBI using CRISPR-Cas9 showed that SlMYBI regulates GABA synthesis by modulating SlGAD1 expression. Furthermore, the interaction of SlNF-YC1 with SlMYBI enhanced the transcriptional activity of SlMYBI on SlGAD1 to further improve saline-alkali tolerance in tomato. Interestingly, we found that ethylene signaling was involved in the GABA response to saline-alkali stress by RNA-seq analysis of SlGAD1-overexpressing lines. This study elucidates the involvement of SlMYBI in GABA synthesis regulation. Specifically, the SlMYBI-SlNF-YC1 module is involved in GABA accumulation in response to saline-alkali stress., Competing Interests: Conflict of interest statement: The authors declare no conflict of interest., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

Domestication has dramatically increased crop size and biomass, reflecting the enhanced accumulation of photosynthates. However, we still lack solid empirical data on the impacts of domestication on photosynthetic rates at different light intensities and on leaf anatomy, and of the relationships of photosynthesis with aboveground biomass. In this study, we measured the photosynthetic rate at three photosynthetic photon flux densities of 2000 (high), 1000 (moderate) and 400 μmol m -2  sec -1 (low light intensity), dark respiration, relative chlorophyll content (SPAD), leaf morphology, and aboveground biomass in 40 wild, 91 semiwild, and 42 domesticated cotton genotypes. The study was replicated for two years (growing years 2018 and 2019). During the first domestication stage (transition from wild to semiwild genotypes), domestication led to higher photosynthetic rates measured under high light intensity, higher SPAD, larger leaf area (LA), and lower leaf mass per unit area (LMA), contributing to greater aboveground biomass accumulation in both study years. During the second domestication stage (transition from semiwild to domesticated genotypes), domestication significantly enhanced photosynthesis under low light intensity and reduced LMA, which were associated with increased aboveground biomass in both study years. In conclusion, photosynthesis improvement at different light intensities has been a gradual domestication phase specific process with the rate of photosynthesis enhanced under high light during the first domestication stage, and under low light during the second domestication stage. We argue that these differences reflect a higher proportion of LA photosynthesizing under low light due to enhanced canopy expansion at the second domestication stage., (© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)

Soil microbial growth, a vital biogeochemical process, governs both the accrual and loss of soil carbon. Here, we investigate the biogeography of soil microbiome potential growth rates and show that microbiomes in resource-rich (high organic matter and nutrients) and acid-neutral soils from cold and humid regions exhibit high potential growth. Conversely, in resource-poor, dry, hot, and hypersaline soils, soil microbiomes display lower potential growth rates, suggesting trade-offs between growth and resource acquisition or stress tolerance. In addition, the potential growth rates of soil microbiomes positively correlates with genome size and the number of ribosomal RNA operons but negatively correlates with optimum temperature, biomass carbon-to-phosphorus and nitrogen-to-phosphorus ratios. The spatial variation of microbial potential growth rates aligns with several macroecological theories. These findings not only enhance our understanding of microbial adaptation to diverse environments but also aid in realistically parameterizing microbial physiology in soil carbon cycling models., (© 2024. The Author(s).)

The endosperm in cereal grains is instrumental in determining grain yield and seed quality, as it controls starch and seed storage protein (SSP) production. In this study, we identified a specific nuclear factor-Y (NF-Y) trimeric complex in wheat (Triticum aestivum L.), consisting of TaNF-YA3-D, TaNF-YB7-B, and TaNF-YC6-B, and exhibiting robust expression within the endosperm during grain filling. Knockdown of either TaNF-YA3 or TaNF-YC6 led to reduced starch but increased gluten protein levels. TaNF-Y indirectly boosted starch biosynthesis genes by repressing TaNAC019, a repressor of cytosolic small ADP-glucose pyrophosphorylase 1a (TacAGPS1a), sucrose synthase 2 (TaSuS2), and other genes involved in starch biosynthesis. Conversely, TaNF-Y directly inhibited the expression of Gliadin-γ-700 (TaGli-γ-700) and low molecular weight-400 (TaLMW-400). Furthermore, TaNF-Y components interacted with SWINGER (TaSWN), the histone methyltransferase subunit of Polycomb repressive complex 2 (PRC2), to repress TaNAC019, TaGli-γ-700, and TaLMW-400 expression through trimethylation of histone H3 at lysine 27 (H3K27me3) modifications. Notably, weak mutation of FERTILIZATION INDEPENDENT ENDOSPERM (TaFIE), a core PRC2 subunit, reduced starch but elevated gliadin and LMW-GS contents. Intriguingly, sequence variation within the TaNF-YB7-B coding region was linked to differences in starch and SSP content. Distinct TaNF-YB7-B haplotypes affect its interaction with TaSWN-B, influencing the repression of targets like TaNAC019 and TaGli-γ-700. Our findings illuminate the intricate molecular mechanisms governing TaNF-Y-PRC2-mediated epigenetic regulation for wheat endosperm development. Manipulating the TaNF-Y complex holds potential for optimizing grain yield and enhancing grain quality., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

Bovine mastitis is regulated by genetic and environmental factors. Long non-coding RNAs (LncRNAs), which regulate various biological processes (immune system and biological development), have been found to play a role in bovine mammary inflammation responses. Here, a novel functional lncRNA, named lncRNA HULIB, was identified as a regulator during bovine mastitis. qRT-PCR and subcellular fractionation assays showed that lncRNA HULIB was significantly up-regulated in LPS-induced bMECs and was mainly localized in the cytoplasm. Gain- or loss-of-function experiments demonstrated that an increase in lncRNA HULIB expression elevated the expression of TLR4 and NF-κB1, which enhanced NF-κB activity, promoting the expression of pro-inflammatory cytokines (IL-6, IL-8, IL-1β, etc) and apoptosis-related genes (BAX, CASP9 and CASP3, etc), while the expression of proliferation-related genes (PCNA, Cyclin D1, Cyclin D2, CDK4 and CDK2) was down-regulated. Ultimately, these changes exacerbated the LPS-induced inflammatory response. Mechanistically, RNA pull-down and RNA immunoprecipitation (RIP) assays revealed that lncRNA HULIB could directly bind the PP2AB protein to regulate inflammatory responses. Overall, lncRNA HULIB is a pro-inflammatory regulator, and its silencing can alleviate the inflammatory responses of bMECs, providing a potential strategy for molecular therapy of bovine mastitis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Phytophthora infestans is a notorious oomycete pathogen that causes potato late blight. It secretes numerous effector proteins to manipulate host immunity. Understanding mechanisms underlying their host cell manipulation is crucial for developing disease resistance strategies. Here, we report that the conserved RXLR effector Pi05910 of P. infestans is a genotype-specific avirulence elicitor on potato variety Longshu 12 and contributes virulence by suppressing and destabilizing host glycolate oxidase StGOX4. By performing co-immunoprecipitation, yeast-two-hybrid assays, luciferase complementation imaging, bimolecular fluorescence complementation and isothermal titration calorimetry assays, we identified and confirmed potato StGOX4 as a target of Pi05910. Further analysis revealed that StGOX4 and its homologue NbGOX4 are positive immune regulators against P. infestans, as indicated by infection assays on potato and Nicotiana benthamiana overexpressing StGOX4 and TRV-NbGOX4 plants. StGOX4-mediated disease resistance involves enhanced reactive oxygen species accumulation and activated the salicylic acid signalling pathway. Pi05910 binding inhibited enzymatic activity and destabilized StGOX4. Furthermore, mutagenesis analyses indicated that the 25th residue (tyrosine, Y25) of StGOX4 mediates Pi05910 binding and is required for its immune function. Our results revealed that the core RXLR effector of P. infestans Pi05910 suppresses plant immunity by targeting StGOX4, which results in decreased enzymatic activity and protein accumulation, leading to enhanced plant susceptibility., (© 2024 The Author(s). Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)