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1. Negative inotropic effect of carbachol and interaction between acetylcholine receptor-operated potassium channel (K.ACh channel) and GTP binding protein in mouse isolated atrium--a novel methodological trial.

Author

Okada M, Noma C, Yamawaki H, and Hara Y

Subjects
Animals, Antibodies, Atropine pharmacology, Bee Venoms pharmacology, Cholinergic Antagonists pharmacology, GTP-Binding Protein beta Subunits genetics, GTP-Binding Protein beta Subunits metabolism, GTP-Binding Proteins genetics, Gene Expression Regulation physiology, Mice, Potassium Channel Blockers pharmacology, Carbachol pharmacology, G Protein-Coupled Inwardly-Rectifying Potassium Channels metabolism, GTP-Binding Proteins metabolism, Heart Atria drug effects, Heart Atria metabolism, Myocardial Contraction drug effects
Abstract

Interaction between acetylcholine receptor-operated potassium channel (K.ACh channel) and GTP binding protein was examined by an immunoprecipitation-Western blotting system in mouse isolated atrium. The carbachol-induced negative inotropic action in indomethacin-pretreated mouse atrium was significantly inhibited by a K.ACh channel blocker, tertiapin or atropine. Kir3.1 K.ACh channel (Kir3.1) was immunoprecipitated with a mouse anti-Kir3.1 antibody. Coprecipitating Gβ with Kir3.1, detected by Western blotting, was significantly augmented by carbachol. Atropine, but not tertiapin, significantly inhibited the carbachol-induced coprecipitating Gβ with Kir3.1. The data indicate that immunoprecipitation with Kir3.1 and Western blotting of Gβ system is a useful method for assessing interaction between K.ACh channel and GTP binding protein in mouse atrium.

Published
2013
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