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Cd accumulation in rice-cultivated soils across China is a major problem that needs to be tackled. A plot experiment was carried out using heavy metal (HM) hyperaccumulators Amaranthus hypochondriacus L. and Perilla frutescens (L.) Britt. intercropped with low-accumulation rice to obtain safe edible rice while reducing the soil Cd concentration. It was found that Cd concentration in soil was decreased by 7.43 and 2.86% under rice intercropped with Amaranthus hypochondriacus L. and Perilla frutescens (L.) Britt., respectively, compared to single cropped rice. In addition, enhanced effects were noted under the combination of Amaranthus hypochondriacus L., Perilla frutescens (L.) Britt, and rice in which a 20.35% decrease in soil Cd content was recorded compared to single-cultivated rice soil. In addition, the available Cd in soil was reduced by 4.00 and 5.00% under rice/ Amaranthus and rice/ Perilla , respectively, and 12.00% under rice/ Amaranthus / Perilla mixed culture. Moreover, the concentration of Cd in various parts of rice was under permissible limits. However, rice biomass was decreased by the presence of hyperaccumulators. This study suggests that combining HM hyperaccumulator plants and low-accumulation rice provides efficient Cd extraction results and could be a crucial option for restoring Cd-contaminated soil without reducing rice production., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)

The practice of returning straw to agricultural fields is a globally employed technique. Such agricultural fields also receive a significant amount of nitrogen (N) and phosphorus (P) fertilizers, because these two macronutrients are essential for plant growth and development. However, the consequences of such macronutrients input on straw decomposition, soil dissolved organic matter (DOM), key microbes, and lignocellulolytic enzymes are still unclear. In a similar aim, we designed a long-term straw returning study without and with different N and P nutrient supplementation: CK (N 0 P 0 ), T1 (N 120 P 0 ), T2 (N 120 P 60 ), T3 (N 120 P 90 ), T4 (N 120 P 120 ), T5 (N 0 P 90 ), T6 (N 60 P 90 ), and T7 (N 180 P 90 ), and evaluated their impact on rice and oilseed rape yield, soil DOM, enzymes, lignocellulose content, microbial diversity, and composition. We found straw returning improved overall yield in all treatments and T7 showed the highest yield for oilseed rape (30.31-38.87 g/plant) and rice (9.14-9.91 t/ha) during five-years of study. The fertilizer application showed a significant impact on soil physicochemical properties, such as water holding capacity and soil porosity decreased, and bulk density increased in fertilized treatments, as compared to CK. Similarly, significantly low OM, cellulose, hemicellulose, and lignin content were found in T7, T4, T3, and T2, while high values were found in CK and T5, respectively. The fluorescence excitation-emission matrix spectra of DOM of different treatments revealed that T3, T7, T4, and T6 showed high peak M (microbial by-products), peak A and peak C (humic acid-like) as compared to others. The microbial composition was also distinctive in each treatment and a high relative abundance of Chloroflexi, Actinobacteriota, Ascomycota, and Basidiomycota were found in T2 and T3 treatments, respectively. These findings indicate that the decomposition of straw in the agricultural field was dependent on nutrients input, which facilitated key microbial growth and impacted positively on lignocellulolytic enzymes, which further aided the breakdown of all components of straw in the field efficiently. On the other hand, high input of chemical based fertilizers to soil can lead to several environmental issues, such as nutrient imbalance, nutrient runoff, soil pH change and changes in microbial activities. Keeping that in consideration, we recommend moderate fertilizer dosage (N 120 P 90 ) in such fields to achieve higher decomposition of crop straw with a small yield compromise., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Anthropogenic activities have raised cadmium (Cd) concentrations in agricultural soil, emerging as a primary catalyst for the decline in crop yield. Intercropping of two or several plants is one technique among many Cd phytoremediation techniques that has gained enormous attention recently. However, the impact of cultivation modes on Cd movement in rice plants when intercropped with heavy metal (HM) hyperaccumulator plants remains unclear. Thus, this study was designed to explore the effects of cultivation modes and the intercropping of rice with Solanum nigrum L. on rice growth and Cd uptake in Cd-contaminated soil. The experimental design encompassed five treatments: dry cultivation of monocultured rice, monocultured Solanum nigrum L., and intercropped rice- Solanum nigrum L.; flood cultivation of monocultured rice; and intercropped rice- Solanum nigrum L. in a high-bed and low-ditch planting system. The results revealed a significant increase in rice growth when intercropped with Solanum nigrum L., with a notable increase of 18.32 g∙plant -1 observed in rice biomass in dry cultivation under the intercropping system. In contrast, a more modest increase of 3.67 g∙plant -1 was observed in the high-bed and low-ditch intercropped rice- Solanum nigrum L. mode. The soil total Cd was higher in dry cultivation of monocultured rice and Solanum nigrum L. compared to intercropped rice/ Solanum nigrum L.-cultivated soil, with lower values recorded for intercropped rice/ Solanum nigrum L. under the high-bed and low-ditch planting system. In contrast, no significant effect was noted on soil exchangeable Cd content based on the planting pattern and cultivation mode. Intercropping with Solanum nigrum L. demonstrated a significant reduction of Cd content in various rice tissues, particularly in roots at the maturity stage, while Cd content was reduced across all rice tissues under the high-bed and low-ditch planting system. The Cd content in the stem, leaves, and bran of monocropped rice was higher compared to intercropped rice. This study suggests that the rice- Solanum nigrum L. intercropping system effectively reduces rice Cd uptake, particularly under the high-bed and low-ditch planting system.

The contamination of saline soil with hazardous petroleum hydrocarbons is a common problem across coastal areas globally. Bioaugmentation combined with chemical treatment is an emerging remediation technique, but it currently shows low efficiency under high saline environments. In this study, we screened and used a novel halotolerant lipolytic fungal consortium (HLFC) combined with hematite (Fe 2 O 3 ) for the bioremediation of diesel contaminated saline soils. The changes in total petroleum hydrocarbons (TPH) concentrations, enzyme activity, and microbial diversity were compared among different treatments (HLFC, hematite, hematite-HLFC, and control). The results showed that TPH degradation was significantly (P < 0.05) enhanced in hematite-HLFC (47.59-88.01%) and HLFC (24.26-72.04%) amended microcosms across all salinity levels, compared to the treatments of hematite (23.71-66.26%) and control (6.39-55.20%). TPH degradation was positively correlated with lipase and laccase enzyme activities, electrical conductivity, and the water holding capacity of the soil. Analyses of the microbial community structure showed that microbial richness decreased, while evenness increased in HLFC and hematite-HLFC treatments. The relative abundances of Alicyclobacillus, Sediminibacillus, Alcanivorax, Penicillium, Aspergillus, and Candida genera were significantly high in hematite-HLFC and HLFC amended microcosms. Our findings provide a promising new microbial-based technique, which can degrade TPH efficiently in saline soil., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Cadmium contamination and toxicity on plants and human health is a major problem in China. Safe rice production in Cd-contaminated alkaline soils, with acceptably low Cd levels and high yields, remains an important research challenge. To achieve this, a small-scale field experiment with seven different soil amendment materials was conducted to test their effects performance. Two best-performing materials were selected for the large-scale field experiment. Combinations of humic acid, foliar, and/or soil silicon fertilization and deep or shallow plowing were designed. It was found that the combination, including humic acid, soil and foliar silicate fertilization, and shallow plowing (5-10 cm), produced the most desirable results (the lowest soil bioavailable Cd, the lowest grain Cd concentrations, and the highest grain yield). Rice farmers are therefore recommended to implement this combination to attain high grain yield with low Cd concentrations in alkaline soils.

The slow decomposition rate of the reluctant structure of lignocellulose in agricultural waste is the great limitation of composting processes, which can be averted by pretreatment-strategies. This study focused on the impacts of pretreating rice straw using a consortium of newly isolated fungal species on lignocellulose degradation and humic substances during composting. Fungal pretreatment had a significant impact on lignocellulose degradation (84%) of rice straw by producing higher lignocellulytic enzymes than chemical pretreatments (79%) or the control (61%). The compost with fungal pretreated rice straw (FPT) showed significantly high composting temperature in the late mesophilic stage, which enhanced the degradation of lignocellulose. The fluorescence excitation emission spectroscopy revealed that significantly more humic acid-like compounds were formed in FPT. These findings suggest that fungal pretreatment is a feasible method to accelerate straw degradation and humification., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Tomato cultivation is affected by high soil salinity and drought stress, which cause major yield losses worldwide. In this work, we compare the efficiency of mechanisms of tolerance to salinity, and osmotic stress applied as mannitol or drought, in three tomato genotypes: LA-2838 (Ailsa Craig), LA-2662 (Saladette), and LA-1996 (Anthocyanin fruit - Aft), a genotype known for high anthocyanin content. Exposure to salinity or drought induced stress in all three genotypes, but the LA-1996 plants displayed superior tolerance to stress compared with the other two genotypes. They were more efficient in anthocyanin and proline accumulation, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity, and leaf Na + , K + , and Ca 2+ homeostasis. In addition, they suffered lesser oxidative damage as measured by chlorophyll (Chl) loss and malondialdehyde (MDA) accumulation, and bioassays showed that they were less affected in terms of seed germination and root elongation. Exposure to stress induced the upregulation of stress-related genes SlNCED1, SlAREB1, SlABF4, SlWRKY8, and SlDREB2A more efficiently in LA-1996 than in the two susceptible genotypes. Conversely, the upregulation of the NADPH oxidase gene SlRBOH1 was more pronounced in LA-2838 and LA-2662. Principal component analysis showed obvious distinction between the tolerant genotype LA-1996 and the susceptible LA-2838 and LA-2662 in response to stress, and association of leaf and stem anthocyanin content with major stress tolerance traits. We suggest that anthocyanin accumulation can be considered as a marker of stress tolerance in tomato, and that LA-1996 can be considered for cultivation in salinity- or drought-affected areas., (Copyright © 2022 Elsevier GmbH. All rights reserved.)

Recently, plant essential oils (EOs) have attracted special attention in plant disease control and food preservation. Since ancient times, essential oils extracted from plants have exhibited many biological characteristics, especially antimicrobial properties. Recent studies have described the potentials of EOs and derivatives to inhibit the growth and reproduction of microorganisms, mainly in response of overwhelming concerns of consumers about food safety. In the context of returning to nature, with the advancement of science and technology and improved living standards, people have begun to seek solutions for food hygiene without chemical additives. Therefore, biological pesticides and plant-oriented chemicals have received special attention from scientists because they are environmentally friendly and nonhazardous, sustainable, and effective alternatives against many noxious phytopathogens. Present study is intended to appraise the fungicidal properties of ginger EOs to combat leaf blight disease of taro, which threatens global taro production. Farmers often hinge on extremely toxic synthetic fungicides to manage diseases, but the residual effects and resistance of chemicals are unavoidable. The microwave-assisted hydrodistillation method was used for ginger EOs extraction and an FTIR (ATR) spectrometer was used to evaluate their chemical composition and citral was identified as most abundant compound (89.05%) in oil. The pathogen isolated from lesions of diseased taro plants was identified as Phytophthora colocasiae and used as test fungus in the present study. Ginger EO was evaluated in-vitro for antifungal properties against mycelium growth, sporangium production, zoospore germination, leaf, and corm necrosis inhibition. Repeated experiments have shown that the concentration of ginger essential oil (1250 ppm) proved to be the lowest dose to obtain 100% inhibition of fungal growth and spore germination, sporangia formation and leaf necrosis assessment. These results are derived from this fungal species and a hypothesis that involves further research on other plant pathogens to demonstrate the overall potency of essential oils. This study references the easy, economic, and environmental management and control of plant diseases using essential oils and byproducts., (© 2022. The Author(s).)

Background: Chemical fertilization helped modern agriculture in grain yield improvement to ensure food security. The response of chemical fertilization for higher hybrid rice production is highly dependent on optimal fertilization management in paddy fields. To assess such responses, in the current work we examine the yield, root growth, and expression of related genes responsible for stress metabolism of nitrogen (N) and phosphorus (P) in two hybrid-rice cultivars Deyou4727 (D47) and Yixiangyou2115 (Y21)., Methods and Results: The experiment followed four nitrogen (N) (N 0 , N 60 , N 120, and N 180 kg/ha) and phosphorus (P) (P 0 , P 60 , P 90 , and P 120 kg/ha) fertilizer levels. The grain yield in D47 was more sensitive to nitrogen application, while Y21 was more sensitive to phosphorus application, which resulted in comparatively higher biomass and yield. Our findings were corroborated by gene expression studies of glutamine synthetase OsGS1;1 and OsGS1;2 and phosphate starvation-related genes PHR1 and SPX, confirming sensitivity to N and P application. The number of roots was less sensitive to nitrogen application in D47 between N 0 and N 60 , but the overall nutrient response difference was significantly higher due to the deep rooting system as compared to Y21., Conclusions: The higher yield, high N and P use efficiency, and versatile root growth of D47 make it suitable to reduce unproductive usage of N and P from paddy fields, improving hybrid rice productivity, and environmental safety in the Sichuan basin area of China., (© 2021. The Author(s).)