Search

Most teleost fish propel themselves with lateral body waves powered by their axial muscles. These muscles also power suction feeding through rapid expansion of the mouth cavity. They consist of muscle segments (myomeres), separated by connective tissue sheets (myosepts). In adult teleosts, the fast axial muscle fibres follow pseudo-helical trajectories, which are thought to distribute strain (relative fibre length change) approximately evenly across transverse sections during swimming, thereby optimizing power generation. To achieve strain equalization, a significant angle to the longitudinal axis on the frontal plane (azimuth) is necessary near the medial plane, increasing strain. Additionally, a deviation from longitudinal orientation on the sagittal plane (elevation) is required laterally to decrease strain. Despite several detailed morphological studies, our understanding of muscle-fibre orientations in the entire axial musculature of fish remains incomplete. Furthermore, most research has been done in post-larval stages, leaving a knowledge gap regarding the changing axial muscle architecture during larval development. Larval fish exhibit different body size, body shape and swimming kinematics compared to adults. They experience relatively high viscous forces, requiring higher tail-beat amplitudes to overcome increased drag. Additionally, larval fish swim with higher tail-beat frequencies. Histological studies have shown that in larval fish, muscle fibres in the anal region transition from an almost longitudinal orientation to a pseudo-helical pattern by 3 dpf (days post-fertilization). However, these studies were limited to a few sections of the body and were prone to shrinkage and tissue damage. Here, we introduce a novel methodology for quantifying muscle-fibre orientations along the entire axial muscles. We selected 4 dpf larval zebrafish for our analyses, a stage where larvae are actively swimming but not yet free-feeding. High-resolution confocal 3D scans were obtained from four genetically modified zebrafish expressing green fluorescent protein in fast muscle fibres. Fluorescence variation allowed segmentation of individual muscle fibres, which were then converted to fish-bound coordinates by correcting for the fish's position and orientation in the scan, and normalized to pool results across individuals. We show that at 4 dpf, muscle-fibre trajectories exhibit a helical pattern tapering towards the tail. Average fibre angles decrease from anterior to posterior, with azimuth varying over the dorsoventral axis and elevation varying over the mediolateral axis. Notably, only the anteriormost 20% of the body displayed higher azimuth angles near the medial plane. Angles between neighbouring fibres were substantial, particularly at the rim of the epaxial and hypaxial muscles. The revealed muscle-fibre architecture at this age presumably contributes to the swimming performance of these larvae, but that swimming performance is probably not the only driving factor for the fibre pattern. Our methodology offers a promising avenue for exploring muscle-fibre orientations across ontogenetic series and provides a foundation for in-depth functional studies on the role of muscle architecture in facilitating swimming performance of larval fish., (© 2024 The Author(s). Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society.)

During the myotomal myogenesis in pike (Esox lucius) two phases of muscle differentiation can be distinguished. In the first phase, the somite cells-derived stock, the primary myoblasts (of mesodermal origin), fuse to form multinucleate myotubes. Participation of myotomal cells of mesodermal origin is insufficient for further muscle development. In the second stage mesenchymal cells migrate, via myosepts, into the myotome between myotubes. Immunocytochemical detection of proliferating cell nuclear antigen (marker of S phase of cell cycle) showed their mitotic activity. Transmission electron microscope analysis revealed that the differentiation of these cells depends on their position. Cells remaining in the myosepts develop into fibroblasts and produce collagen fibres, while those that have migrated into the myotomes transform into secondary myoblasts. Mesenchymal cells in the studied species are believed to participate in hypertrophy and hyperplasy of muscle fibres. Thus the muscle fibres in pike (E. lucius) are of mesodermal-mesenchymal origin.

The formation of myosepts of the toad is characterized by three stages in early development. At the first stage, the somitic cells give off some pseudopods and many collagen fibres. At the second stage, the newwork of collagen fibers grows denser but pseudopods retract. In the end, at the third stage, the collagen fibres disappear and secreting fibroblasts are seen in the myosepts.

The distribution of intercellular junctions between premyotomal and myotomal cells of Xenopus embryos has been described. Intermediate junctions, gap junctions, and small desmosomes are found between unsegmented mesoderm cells. In the region of myotome formation gap junctions appear to be absent, although close appositions of cell membranes are found. Both gap and intermediate junctions are numerous between cells of the segmented myotomes; they occur within the myotomes close to the myosepts and at interdigitations between the myotomes across the myosepts. Gap junctions between the myotomes are found in embryos that have hatched and can swim. The observed distribution of gap junctions is correlated with physiological data on electrical coupling. Electrical connections via gap junctions may produce contraction of the axial musculature remote from motor innervation.