1. CdSe/ZnS quantum dots induce photodynamic effects and cytotoxicity in pancreatic cancer cells.
- Author
-
He SJ, Cao J, Li YS, Yang JC, Zhou M, Qu CY, Zhang Y, Shen F, Chen Y, Li MM, and Xu LM
- Subjects
- Apoptosis drug effects, Apoptosis Regulatory Proteins genetics, Apoptosis Regulatory Proteins metabolism, Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Dose-Response Relationship, Drug, Flow Cytometry, Gene Expression Regulation, Neoplastic, Humans, Microscopy, Electron, Transmission, Microscopy, Fluorescence, Oxidative Stress drug effects, Pancreatic Neoplasms genetics, Pancreatic Neoplasms metabolism, Pancreatic Neoplasms ultrastructure, RNA, Messenger genetics, RNA, Messenger metabolism, Reactive Oxygen Species metabolism, Time Factors, Cadmium Compounds pharmacology, Pancreatic Neoplasms drug therapy, Photochemotherapy, Photosensitizing Agents pharmacology, Quantum Dots, Selenium Compounds pharmacology, Sulfides pharmacology, Zinc Compounds pharmacology
- Abstract
Aim: To investigate the photodynamic effect of CdSe/ZnS quantum dots (QDs) on pancreatic cancer cells and elucidate the probable mechanisms., Methods: The pancreatic cancer cell line SW1990 was treated with different concentrations of CdSe/ZnS QDs (0, 0.5, 1.0, 1.5, 2.0, 2.5 μmol/L), with or without illumination. The viability of SW1990 cells was tested using the Cell Counting Kit-8 (CCK-8) assay. The ultrastructural changes of SW1990 cells were observed by transmission electron microscopy. Apoptosis was detected by nuclear staining and flow cytometry (FCM). Reactive oxygen species (ROS) were measured by dichlorofluorescein diacetate via fluorescence microscopy. Expression of Bax, Bcl-2 and caspase-3 was measured by real-time polymerase chain reaction (PCR) and protein immunoblotting 24 h after SW1990 cells were treated with CdSe/ZnS QDs and illuminated., Results: The CCK-8 assay results showed that both CdSe/ZnS QDs with and without illumination suppressed SW1990 cell proliferation. Cell viability was significantly lower when illuminated or with a longer incubation time and a higher light dose. CdSe/ZnS QDs with illumination caused ultrastructural changes in SW1990 cells, such as organelle degeneration and chromatin condensation and aggregation at the periphery of the nucleus. Fluorescence microscopy and FCM showed that CdSe/ZnS QDs (1.5 μmol/L) with illumination increased SW1990 cell apoptosis (53.2%) and ROS generation compared with no illumination. Real-time PCR showed that expression of Bax and caspase-3 was upregulated and Bcl-2 was downregulated. Immunoblotting results were consistent with real-time PCR results. Inhibition of ROS and apoptosis both attenuated QD-photodynamic-therapy-induced cell death., Conclusion: CdSe/ZnS QDs can be used as a photosensitizer to inhibit SW1990 cell proliferation through ROS generation and apoptotic protein expression regulation.
- Published
- 2016
- Full Text
- View/download PDF