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The aim of this study was to prevent initial changes in Drosophila melanogaster oocytes under simulated weightlessness and hypergravity at the 2 g level. Phospholipids with polyunsaturated fatty acids in the tail groups (essential phospholipids) at a concentration of 500 mg/kg of nutrient medium were used as a protective agent. Cell stiffness was determined using atomic force microscopy, the change in the oocytes' area was assessed as a mark of deformation, and the contents of cholesterol and neutral lipids were determined using fluorescence microscopy. The results indicate that the administration of essential phospholipids leads to a decrease in the cholesterol content in the oocytes' membranes by 13% ( p < 0.05). The stiffness of oocytes from flies that received essential phospholipids was 14% higher ( p < 0.05) and did not change during 6 h of simulated weightlessness or hypergravity, and neither did the area, which indicates their resistance to deformation. Moreover, the exposure to simulated weightlessness and hypergravity of oocytes from flies that received a standard nutrient medium led to a more intense loss of cholesterol from cell membranes after 30 min by 13% and 18% ( p < 0.05), respectively, compared to the control, but essential phospholipids prevented this effect.

Purpose: Quantitative sensory testing commonly utilizes the unaffected, contralateral side as a control to detect somatosensory dysfunction. There is scant evidence that somatosensory function for the volar dominant and non-dominant hands is equivalent, therefore intra-patient comparisons are unwarranted. This study aimed to identify dominance-related differences in palmar hand somatosensation, thereby determining if the unaffected contralateral hand is a valid comparator in clinical populations., Participants and Methods: With ethical approval (IREC&#95;13&#95;1&#95;10) and informed consent, 110 healthy adult volunteers' participated in this clinical measurement study. Somatosensory function was assessed with the German Research Network on Neuropathic Pain (DFNS) quantitative sensory testing (QST) protocol. Half of the participants were tested on the dominant hand. Thirteen parameters of thermal and mechanical detection and pain threshold were evaluated at both the dorsal and volar hand (distal middle finger). Tests were performed in the same order and instructions were read from a standardized script. Results for dorsal hand tests were compared to DFNS normative data to confirm participants met study inclusion criteria. Between-group differences for age and sex were investigated with the independent samples t -test and Chi-square test of independence, respectively. Group differences for dominant and non-dominant hands for all 13 continuous QST parameters were investigated with the Mann-Whitney U -test., Results: Data for 106 participants were included in statistical analysis. Fifty percent of participants were tested on the dominant hand [n=53]; there were no differences for age or sex between groups (dominant or non-dominant hand test group). The dominant volar hand was significantly more sensitive to vibration detection threshold than the non-dominant hand (P=0.001). There were no significant differences related to dominance for other DFNS QST measures., Conclusion: For quantitative sensory testing with the DFNS protocol in healthy cohorts, the contralateral, unaffected hand is a valid control, with the exception of vibration detection threshold., Competing Interests: ASCR undertakes consultancy and advisory board work for Imperial College Consultants. In the last 12 months this has included remunerated work for Pharmnovo, Confo, Combigene, lateral and Astra Zeneca. He is Officer (President-Elect) of International Association for the Study of Pain. He reports a member of Joint Committee for Vaccine and Immunisation – varicella sub-committee; Analgesic Clinical Trial Translation: Innovations, Opportunities, and Networks (ACTTION) steering committee member; Medicines and Healthcare products Regulatory Agency (MHRA); Commission on Human Medicines – Neurology, Pain & Psychiatry Expert Advisory Group; he also reports grants from and studentships of UKRI (Medical Research Council & BBSRC), Versus Arthritis, Alan and Sheila Diamond Trust, Royal British Legion, European Commission, Ministry of Defence, Dr Jennie Gwynn Bequests, The British Pain Society, Royal Society of Medicine. The authors report no other conflicts of interest in this work., (© 2024 Kennedy et al.)

Solitary bees play a crucial role in ecological systems, contributing to the pollination of crops and wild plants. All females are reproductive, and their habitat requirements include nesting sites, food resources and nesting materials. Although these activities require the ability to detect biotic and abiotic stimuli in the environment, the sensory system of these species is poorly studied. In this study, the antennal sensilla of five solitary bee species belonging to three Apoidea families were investigated using scanning electron microscopy. These included two species of stem-nesting bees, Ceratina cucurbitina (Rossi, 1792) (Apidae) and Osmia scutellaris (Morawitz, 1868) (Megachilidae), and three species of ground-nesting bees, Lasioglossum brevicorne (Schenck, 1870), Lasioglossum leucozonium (Schrank, 1781), and Lasioglossum villosulum (Kirby, 1802) (Halictidae). Thirteen different types of antennal sensilla were identified in females based on their morphological characteristics: sensilla trichodea (subtypes STI, II, III), chaetica (subtypes SchI, II), basiconica (subtypes SBI, II, III, IV), placodea, campaniformia, coeloconica, and ampullacea. Their functional role was discussed and morphology was compared among the species and within the antennal segments in each species. The results provide a baseline for further physiological and behavioural studies to determine the role of antennal sensilla in habitat selection, food search and nesting site selection., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Octopuses integrate visual, chemical and tactile sensory information while foraging and feeding in complex marine habitats. The respective roles of these modes are of interest ecologically, neurobiologically, and for development of engineered soft robotic arms. While vision guides their foraging path, benthic octopuses primarily search "blindly" with their arms to find visually hidden prey amidst rocks, crevices and coral heads. Each octopus arm is lined with hundreds of suckers that possess a combination of chemo- and mechanoreceptors to distinguish prey. Contact chemoreception has been demonstrated in lab tests, but mechanotactile sensing is less well characterized. We designed a non-invasive live animal behavioral assay that isolated mechanosensory capabilities of Octopus bimaculoides arms and suckers to discriminate among five resin 3D-printed prey and non-prey shapes (all with identical chemical signatures). Each shape was introduced inside a rock dome and was only accessible to the octopus' arms. Octopuses' responses were variable. Young octopuses discriminated the crab prey shape from the control, whereas older octopuses did not. These experiments suggest that mechanotactile sensing of 3D shapes may aid in prey discrimination; however, (i) chemo-tactile information may be prioritized over mechanotactile information in prey discrimination, and (ii) mechanosensory capability may decline with age., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

A trichobothrium is a complex sensory organ, which usually consists of a long, slender mechanoreceptive seta (trich), which is situated in a cuplike depression in the cuticle (bothrium). Nabidae (Hemiptera: Heteroptera: Cimicomorpha), also called damsel bugs, are a relatively small family within which two subfamilies, Nabinae and Prostemmatinae, are distinguished. Trichobothria are present in the number of one to seven pairs located laterally on the scutellum of adult representatives of Prostemmatinae. This feature is commonly used to distinguish this subfamily from Nabinae. Trichobothria are also found on the abdominal tergites of Prostemmatinae nymphs. Similar sensilla have been observed in adult representatives of Nabinae, but their homology has not yet been confirmed. During morphological studies on Nabidae, conducted using scanning electron microscopy, we noticed sensilla resembling trichobothria on the heads of these insects. This discovery prompted us to examine the presence of these structures in damsel bugs more carefully. Imagines of fifteen species of both subfamilies were analysed using a scanning electron microscope. The results present data on the distribution and micromorphology of the trichobothria in damsel bugs. A pair of dorsal and ventral cephalic trichobothria were observed in all of the examined species of subfamily Nabinae. These sensilla were not found on the heads of Prostemmatinae. The results of studies on scutellar trichobothria confirmed the previously known data regarding their occurrence in Prostemmatinae. Moreover, our research showed the presence of these sensory structures in all of the examined Nabinae species: one pair of trichobothria in Arachnocorini, Carthasini, Gorpini and Nabini, and two pairs in Stenonabini. The presence of abdominal trichobothria was shown in Nabini and Stenonabini. In the remaining studied tribes of Nabinae and in the subfamily Prostemmatinae, the presence of structures that could undoubtedly be considered abdominal trichobothria was not found., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Surface-feeding aquatic animals navigate towards the source of water disturbances and must differentiate prey from other environmental stimuli. Medicinal leeches locate prey, in part, using a distribution of mechanosensory hairs along their body that deflect under fluid flow. Leech's behavioral responses to surface wave temporal frequency are well documented. However, a surface wave's temporal frequency depends on many underlying environmental and fluid properties that vary substantially in natural habitats (e.g., water depth, temperature). The impact of these variables on neural response and behavior is unknown. Here, we developed a physics-based leech mechanosensor model to examine the impact of environmental and fluid properties on neural response. Our model used the physical properties of a leech cilium and was verified against existing behavioral and electrophysiological data. The model's peak response occurred with waves where the effects of gravity and surface tension were nearly equal (i.e., the phase velocity minimum). This suggests that preferred stimuli are related to the interaction between fundamental properties of the surrounding medium and the mechanical properties of the sensor. This interaction likely tunes the sensor to detect the nondispersive components of the signal, filtering out irrelevant ambient stimuli, and may be a general property of cilia across the animal kingdom., Competing Interests: Declaration of competing interest None, (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

It is well-recognized that blood flow at branches and bends of arteries generates disturbed shear stress, which plays a crucial in driving atherosclerosis. Flow-generated fluid shear stress (FSS), as one of the key hemodynamic factors, is appreciated for its critical involvement in regulating angiogenesis to facilitate wound healing and tissue repair. Endothelial cells can directly sense FSS but the mechanobiological mechanism by which they decode different patterns of FSS to trigger angiogenesis remains unclear. In the current study, laminar shear stress (LSS, 15 dyn/cm 2 ) was employed to mimic physiological blood flow, while disturbed shear stress (DSS, ranging from 0.5 ± 4 dyn/cm 2 ) was applied to simulate pathological conditions. The aim was to investigate how these distinct types of blood flow regulated endothelial angiogenesis. Initially, we observed that DSS impaired angiogenesis and downregulated endogenous vascular endothelial growth factor B (VEGFB) expression compared to LSS. We further found that the changes in membrane protein, migration and invasion enhancer 1 (MIEN1) play a role in regulating ERK/MAPK signaling, thereby contributing to endothelial angiogenesis in response to FSS. We also showed the involvement of MIEN1-directed cytoskeleton organization. These findings suggest the significance of shear stress in endothelial angiogenesis, thereby enhancing our understanding of the alterations in angiogenesis that occur during the transition from physiological to pathological blood flow., (© 2024 Wiley Periodicals LLC.)

Chemical-based interventions are mostly used to control insects that are harmful to human health and agriculture or that simply cause a nuisance. An overreliance on these insecticides however raises concerns for the environment, human health, and the development of resistance, not only in the target species. As such, there is a critical need for the development of novel nonchemical technologies to control insects. Electrocution traps using UV light as an attractant are one classical nonchemical approach to insect control but lack the specificity necessary to target only pest insects and to avoid harmless or beneficial species. Here we review the fundamental physics behind electric fields (EFs) and place them in context with electromagnetic fields more broadly. We then focus on how novel uses of strong EFs, some of which are being piloted in the field and laboratory, have the potential to repel, capture, or kill (electrocute) insects without the negative side effects of other classical approaches. As EF-insect science remains in its infancy, we provide recommendations for future areas of research in EF-insect science., (© The Author(s) 2024. Published by Oxford University Press on behalf of Entomological Society of America.)

It is recognized that the changes in the physical properties of extracellular matrix (ECM) result in fine-tuned cell responses including cell morphology, proliferation and differentiation. In this study, a novel patterned equidistant micropillar substrate based on polydimethylsiloxane (PDMS) is designed to mimic the collagen fiber-like network of the cartilage matrix. By changing the component of the curing agent to an oligomeric base, micropillar substrates with the same topology but different stiffnesses are obtained and it is found that chondrocytes seeded onto the soft micropillar substrate maintain their phenotype by gathering type II collagen and aggrecan more effectively than those seeded onto the stiff micropillar substrate. Moreover, chondrocytes sense and respond to micropillar substrates with different stiffnesses by altering the ECM-cytoskeleton-focal adhesion axis. Further, it is found that the soft substrate-preserved chondrocyte phenotype is dependent on the activation of Wnt/β-catenin signaling. Finally, it is indicated that the changes in osteoid-like region formation and cartilage phenotype loss in the stiffened sclerotic area of osteoarthritis cartilage to validate the changes triggered by micropillar substrates with different stiffnesses. This study provides the cell behavior changes that are more similar to those of real chondrocytes at tissue level during the transition from a normal state to a state of osteoarthritis., (© 2023 Wiley-VCH GmbH.)

This study was conducted to determine the senses that facilitate prey detection in the marble goby (Oxyeleotris marmorata) larvae. The ingestion ratios of live (generate chemical and mechanical stimuli) or frozen Artemia nauplii (generate chemical but no mechanical stimuli) by the intact or free neuromast (mechanoreceptor)-ablated O. marmorata larvae (11 mg/L streptomycin treatment before feeding) under the light or dark (fish vision was obstructed) condition were examined. Vision, mechano-, and chemoreceptions were all found to be essential in prey detection of the O. marmorata larvae. Prey movement has a significant influence as a visual stimuli on the O. marmorata larval feeding as the Artemia nauplii ingestion ratio was approximately 40% higher with significant (p = 0.001, d = 3.0), when the intact larvae were fed with the live (78.1 ± 1.5%), rather than the frozen (40.9 ± 2.8%) Artemia nauplii, under the light condition. This result was assured when no significant difference (p = 0.572, d = 0.2) was found between the ingestion ratios of frozen Artemia nauplii by the intact O. marmorata larvae under light and dark conditions. These findings demonstrate that prey detection in the O. marmorata larvae was facilitated by multi-modal senses, allowing O. marmorata larvae to survive in their natural habitats., (Copyright © 2023 Elsevier B.V. All rights reserved.)

VLGR1/ADGRV1 (very large G protein-coupled receptor-1) is the largest adhesion G protein-coupled receptor (aGPCR). Mutations in VLGR1/ADGRV1 are associated with human Usher syndrome, the most common form of deaf-blindness, and also with epilepsy in humans and mice. VLGR1 is expressed almost ubiquitously but is mainly found in the CNS and in the sensory cells of the eye and inner ear. Little is known about the pathogenesis of the diseases related to VLGR1. We previously identified VLGR1 as a vital component of focal adhesions (FAs) serving as a metabotropic mechanoreceptor controls cell spreading and migration. FAs are highly dynamic and turnover in response to internal and external signals. Here, we aimed to elucidate how VLGR1 participates in FA turnover. Nocodazole washouts and live cell imaging of paxillin-DsRed2 consistently showed that FA disassembly was not altered, but de novo assembly of FA was significantly delayed in Vlgr1-deficient astrocytes, indicating that VLGR1 is enrolled in FA assembly. In FRAP experiments, recovery rates were significantly reduced in Vlgr1-deficient FAs, indicating reduced turnover kinetics in VLGR1-deficient FAs. We showed that VLGR1 regulates cell migration by controlling the FA turnover during their assembly and expect novel insights into pathomechanisms related to pathogenic dysfunctions of VLGR1., (© 2023 The Authors. Basic & Clinical Pharmacology & Toxicology published by John Wiley & Sons Ltd on behalf of Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society).)

It is a big challenge to design a biomimetic physical microenvironment with greater similarity to in vivo tissue to observe real cell behaviors. We established a novel cell culture platform based on patterned equidistant micropillars with stiff and soft stiffnesses to mimic the changes that happened in the transition from normal to osteoporotic disease. We first demonstrated that the soft micropillar substrate decreased osteocyte synaptogenesis through synaptogyrin 1 and that this decrease was accompanied by impairment of cell mechanoperception and a decrease in cellular cytoskeletal rearrangement. We then found that the soft equidistant micropillar substrate reduced the osteocyte synaptogenesis mainly via the inactivation of Erk/MAPK signaling. We finally found that soft micropillar substrate-mediated synaptogenesis impacted the cell-to-cell communication and matrix mineralization of osteocytes. Taken together, this study provides evidence of cellular mechanical responses that are much more similar to those of real osteocytes at the bone tissue level.

One of the largest ecological transitions in carnivoran evolution was the shift from terrestrial to aquatic lifestyles, which has driven morphological diversity in skulls and other skeletal structures. In this paper, we investigate the association between those lifestyles and whisker morphology. However, comparing whisker morphology over a range of species is challenging since the number of whiskers and their positions on the mystacial pads vary between species. Also, each whisker will be at a different stage of growth and may have incurred damage due to wear and tear. Identifying a way to easily capture whisker morphology in a small number of whisker samples would be beneficial. Here, we describe individual and species variation in whisker morphology from two-dimensional scans in red fox, European otter and grey seal. A comparison of long, caudal whiskers shows inter-species differences most clearly. We go on to describe global whisker shape in 24 species of carnivorans, using linear approximations of curvature and taper, as well as traditional morphometric methods. We also qualitatively examine surface texture, or the presence of scales, using scanning electron micrographs. We show that gross whisker shape is highly conserved, with whisker curvature and taper obeying simple linear relationships with length. However, measures of whisker base radius, length, and maybe even curvature, can vary between species and substrate preferences. Specifically, the aquatic species in our sample have thicker, shorter whiskers that are smoother, with less scales present than those of terrestrial species. We suggest that these thicker whiskers may be stiffer and able to maintain their shape and position during underwater sensing, but being stiffer may also increase wear., (© 2023 The Authors. Journal of Morphology published by Wiley Periodicals LLC.)

Articular cartilage, composed of collagen type II as a major extracellular matrix and chondrocyte as a unique cell type, is a specialized connective tissue without blood vessels, lymphatic vessels and nerves. This distinctive characteristic of articular cartilage determines its very limited ability to repair when damaged. It is well known that physical microenvironmental signals regulate many cell behaviors such as cell morphology, adhesion, proliferation and cell communication even determine chondrocyte fate. Interestingly, with increasing age or progression of joint diseases such as osteoarthritis (OA), the major collagen fibrils in the extracellular matrix of articular cartilage become larger in diameter, leading to stiffening of articular tissue and reducing its resistance to external tension, which in turn aggravates joint damage or progression of joint diseases. Therefore, designing a physical microenvironment closer to the real tissue and thus obtaining data closer to the real cellular behaviour, and then revealing the biological mechanisms of chondrocytes in pathological states is of crucial importance for the treatment of OA disease. Here we fabricated micropillar substrates with the same topology but different stiffnesses to mimic the matrix stiffening that occurs in the transition from normal to diseased cartilage. It was first found that chondrocytes responded to stiffened micropillar substrates by showing a larger cell spreading area, a stronger enhancement of cytoskeleton rearrangement and more stability of focal adhesion plaques. The activation of Erk/MAPK signalling in chondrocytes was detected in response to the stiffened micropillar substrate. Interestingly, a larger nuclear spreading area of chondrocytes at the interface layer between the cells and top surfaces of micropillars was observed in response to the stiffened micropillar substrate. Finally, it was found that the stiffened micropillar substrate promoted chondrocyte hypertrophy. Taken together, these results revealed the cell responses of chondrocytes in terms of cell morphology, cytoskeleton, focal adhesion, nuclei and cell hypertrophy, and may be beneficial for understanding the cellular functional changes affected by the matrix stiffening that occurs during the transition from a normal state to a state of osteoarthritis., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)

The subgenual organ complex in the leg of Polyneoptera (Insecta) consists of several chordotonal organs specialized to detect mechanical stimuli from substrate vibrations and airborne sound. In stick insects (Phasmatodea), the subgenual organ complex contains the subgenual organ and the distal organ located distally to the subgenual organ. The subgenual organ is a highly sensitive detector for substrate vibrations. The distal organ has a characteristic linear organization of sensilla and likely also responds to substrate vibrations. Despite its unique combination of sensory organs, the neuroanatomy of the subgenual organ complex of stick insects has been investigated for only very few species so far. Phylogenomic analysis has established for Phasmatodea the early branching of the sister groups Oriophasmata, the Old World phasmids, and Occidophasmata, the New World phasmids. The species studied for the sensory neuroanatomy, including the Indian stick insect Carausius morosus, belong to the Old World stick insects. Here, the neuroanatomy of the subgenual organ complex is presented for a first species of the New World stick insects, the Peruvian stick insect Oreophoetes peruana. To document the sensory organs in the subgenual organ complex and their innervation pattern, and to compare these between females and males of this species and also to the Old World stick insects, axonal tracing is used. This study documents the same sensory organs for O. peruana, subgenual organ and distal organ, as in other stick insects. Between the sexes of this species, there are no notable differences in the neuroanatomy of their sensory organs. The innervation pattern of tibial nerve branches in O. peruana is identical to other stick insect species, although the innervation pattern of the subgenual organ by a single tibial nerve branch is simpler. The shared organization of the organs in the subgenual organ complex in both groups of Neophasmatodea (Old World and New World stick insects) indicates the sensory importance of the subgenual organ but also of the distal organ. Some variation exists in the innervation of the chordotonal organs in O. peruana though a common innervation pattern can be identified. The findings raise the question for the ancestral neuroanatomical organization and innervation in stick insects., (© 2022 The Author(s). Published by S. Karger AG, Basel.)

The lack of gravitational loading is a pivotal risk factor during space flights. Biomedical studies indicate that because of the prolonged effect of microgravity, humans experience bone mass loss, muscle atrophy, cardiovascular insufficiency, and sensory motor coordination disorders. These findings demonstrate the essential role of gravity in human health quality. The physiological and pathophysiological mechanisms of an acute response to microgravity at various levels (molecular, cellular, tissue, and physiological) and subsequent adaptation are intensively studied. Under the permanent gravity of the Earth, multicellular organisms have developed a multi-component tissue mechanosensitive system which includes cellular (nucleo- and cytoskeleton) and extracellular (extracellular matrix, ECM) "mechanosensory" elements. These compartments are coordinated due to specialized integrin-based protein complexes, forming a distinctive mechanosensitive unit. Under the lack of continuous gravitational loading, this unit becomes a substrate for adaptation processes, acting as a gravisensitive unit. Since the space flight conditions limit large-scale research in space, simulation models on Earth are of particular importance for elucidating the mechanisms that provide a response to microgravity. This review describes current state of art concerning mammalian ECM as a gravisensitive unit component under real and simulated microgravity and discusses the directions of further research in this field.

In arthropods, the detection of vibrational signals and stimuli is essential in several behaviours, including mate recognition and pair formation, prey detection, and predator evasion. These behaviours have been studied in several species of insects, arachnids, and crustaceans for vibration production and propagation in the environment. Vibration stimuli are transferred over the animals' appendages and the body to vibrosensory organs. Ultimately, the stimuli are transferred to act on the dendrites of the mechanosensitive sensilla. We refer to these two different levels of transfer as macromechanics and micromechanics, respectively. These biomechanical processes have important roles in filtering and pre-processing of stimuli, which are not carried out by neuronal components of sensory organs. Also, the macromechanical transfer is posture-dependent and enables behavioural control of vibration detection. Diverse sensory organs respond to vibrations, including cuticular sensilla (slit sensilla, campaniform sensilla) and internal chordotonal organs. These organs provide various adaptations, as they occur at diverse body positions with different mechanical couplings as input pathways. Macromechanics likely facilitated evolution of vibrosensory organs at specific body locations. Thus, vibration detection is a highly complex sensory capacity, which employs body and sensory mechanics for signal filtering, amplification, and analysis of frequency, intensity and directionality., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Spiders show a broad range of motions in addition to walking and running with their eight coordinated legs taking them towards their resources and away from danger. The usefulness of all these motions depends on the ability to control and adjust them to changing environmental conditions. A remarkable wealth of sensory receptors guarantees the necessary guidance. Many facets of such guidance have emerged from neuroethological research on the wandering spider Cupiennius salei and its allies, although sensori-motor control was not the main focus of this work. The present review may serve as a springboard for future studies aiming towards a more complete understanding of the spider's control of its different types of motion. Among the topics shortly addressed are the involvement of lyriform slit sensilla in path integration, muscle reflexes in the walking legs, the monitoring of joint movement, the neuromuscular control of body raising, the generation of vibratory courtship signals, the sensory guidance of the jump to flying prey and the triggering of spiderling dispersal behavior. Finally, the interaction of sensors on different legs in oriented turning behavior and that of the sensory systems for substrate vibration and medium flow are addressed.

It has long been assumed that the elongated rostra (the saws) of sawsharks (family: Pristiophoridae) and sawfish (family: Pristidae) serve a similar function. Recent behavioural and anatomical studies have shed light on the dual function of the pristid rostrum in mechanosensory and electrosensory prey detection and prey manipulation. Here, the authors examine the distributions of the mechanosensory lateral line canals and electrosensory ampullae of Lorenzini in the southern sawshark, Pristiophorus nudipinnis and the longnose sawshark, Pristiophorus cirratus. In both species, the receptive fields of the mechano- and electrosensory systems extend the full length of the rostrum indicating that the sawshark rostrum serves a sensory function. Interestingly, despite recent findings suggesting they feed at different trophic levels, minimal interspecific variation between the two species was recorded. Nonetheless, compared to pristids, the pristiophorid rostrum possesses a reduced mechanosensory sampling field but higher electrosensory resolution, which suggests that pristiophorids may not use their rostrums to disable large prey like pristids do., (© 2020 Fisheries Society of the British Isles.)

Dragonflies are hemimetabolous insects, switching from an aquatic life style as nymphs to aerial life as adults, confronted to different environmental cues. How sensory structures on the antennae and the brain regions processing the incoming information are adapted to the reception of fundamentally different sensory cues has not been investigated in hemimetabolous insects. Here we describe the antennal sensilla, the general brain structure, and the antennal sensory pathways in the last six nymphal instars of Libellula depressa , in comparison with earlier published data from adults, using scanning electron microscopy, and antennal receptor neuron and antennal lobe output neuron mass-tracing with tetramethylrhodamin. Brain structure was visualized with an anti-synapsin antibody. Differently from adults, the nymphal antennal flagellum harbors many mechanoreceptive sensilla, one olfactory, and two thermo-hygroreceptive sensilla at all investigated instars. The nymphal brain is very similar to the adult brain throughout development, despite the considerable differences in antennal sensilla and habitat. Like in adults, nymphal brains contain mushroom bodies lacking calyces and small aglomerular antennal lobes. Antennal fibers innervate the antennal lobe similar to adult brains and the gnathal ganglion more prominently than in adults. Similar brain structures are thus used in L. depressa nymphs and adults to process diverging sensory information.

While chemical communication has been investigated intensively in vertebrates and insects, relatively little is known about the sensory world of spiders despite the fact that chemical cues play a key role in natural and sexual selection in this group. In insects, olfaction is performed with wall-pore and gustation with tip-pore sensilla. Since spiders possess tip-pore sensilla only, it is unclear how they accomplish olfaction. We scrutinized the ultrastructure of the trichoid tip-pore sensilla of the orb weaving spider Argiope bruennichi-a common Palearctic species the males of which are known to be attracted by female sex pheromone. We also investigated the congener Argiope blanda. We examined whether the tip-pore sensilla differ in ultrastructure depending on sex and their position on the tarsi of walking legs of which only the distal parts are in contact with the substrate. We hypothesized as yet undetected differences in ultrastructure that suggest gustatory versus olfactory functions. All tarsal tip-pore sensilla of both species exhibit characters typical of contact-chemoreceptors, such as (a) the presence of a pore at the tip of the sensillum shaft, (b) 2-22 uniciliated chemoreceptive cells with elongated and unbranched dendrites reaching up to the tip-pore, (c) two integrated mechanoreceptive cells with short dendrites and large tubular bodies attached to the sensillum shaft's base, and (d) a socket structure with suspension fibres that render the sensillum shaft flexible. The newly found third mechanoreceptive cell attached to the proximal end of the peridendritic shaft cylinder by a small tubular body was likely overlooked in previous studies. The organization of tarsal tip-pore sensilla did not differ depending on the position on the tarsus nor between the sexes. As no wall-pore sensilla were detected, we discuss the probability that a single type of sensillum performs both gustation and olfaction in spiders., (© 2020 The Authors. Journal of Morphology published by Wiley Periodicals LLC.)

Whiskers are present in many species of mammals. They are specialised vibrotactile sensors that sit within strongly innervated follicles. Whisker size and shape will affect the mechanical signals that reach the follicle, and hence the information that reaches the brain. However, whisker size and shape have not been quantified across mammals before. Using a novel method for describing whisker curvature, this study quantifies whisker size and shape across 19 mammalian species. We find that gross two-dimensional whisker shape is relatively conserved across mammals. Indeed, whiskers are all curved, tapered rods that can be summarised by Euler spiral models of curvature and linear models of taper, which has implications for whisker growth and function. We also observe that aquatic and semi-aquatic mammals have relatively thicker, stiffer, and more highly tapered whiskers than arboreal and terrestrial species. In addition, smaller mammals tend to have relatively long, slender, flexible whiskers compared to larger species. Therefore, we propose that whisker morphology varies between larger aquatic species, and smaller scansorial species. These two whisker morphotypes are likely to induce quite different mechanical signals in the follicle, which has implications for follicle anatomy as well as whisker function., (© 2020 The Authors. Journal of Morphology published by Wiley Periodicals LLC.)

Stick insects have elaborate mechanosensory organs in their subgenual organ complex in the proximal tibia, particularly the distal organ with scolopidial sensilla in linear arrangement. For early postembryonic developmental stages of Sipyloidea sipylus (Phasmatodea: Necrosciinae), the neuroanatomy of the scolopidial organs in the subgenual organ complex and the campaniform sensilla is documented by retrograde axonal tracing, and compared to the adult neuroanatomy. Already after hatching of the first larval instars are the sensory structures of subgenual organ and distal organ as well as tibial campaniform sensilla differentiated. In the distal organ, the full set of sensilla is shown in all larval stages examined. This finding indicates that the sensory organs differentiate during embryogenesis, and are already functional by the time of hatching. The constancy of distal organ sensilla over postembryonic stages allows investigation of the representative number of sensilla in adult animals as well as in larval instars. Some anatomical changes occur by postembryogenic length increase of the distal organ, and grouping of the anterior subgenual sensilla. The embryonic development of scolopidial sensilla is similar for auditory sensilla in hemimetabolous Orthoptera (locusts, bushcrickets, crickets) where tympanal membranes develop during postembryogenic stages, conferring a successive gain of sensitivity with larval moults., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Juvenile Nile crocodiles (Crocodylus niloticus) and spectacled caimans (Caiman crocodilus) use water surface waves for the detection of prey, usually insects trapped at the water surface. This prey detection relies on mechanosensors, the integumentary sensory organs. We found by go/no go conditioning that C. niloticus and C. crocodilus can discriminate surface waves that differ in frequency. On average, frequency difference thresholds were about 4-5 %, e.g. C. niloticus and C. crocodilus distinguished a 40 Hz surface wave from a 38,5 Hz surface wave stimulus. C. niloticus and C. crocodilus also discriminated between single-frequency surface waves (15 Hz or 40 Hz) and surface waves that showed an abrupt frequency change (e.g. from 15 to 16.5 Hz or from 40 Hz to 38.5 Hz). The threshold for the abrupt frequency changes averaged 3-9 %. Additionally, Nile crocodiles differentiated also between a single-frequency water surface wave and a water surface wave that was amplitude modulated. C. niloticus also determined the direction (mean error angle between 13,7° and 16,6°) to a surface wave stimulus. Furthermore, the distance covered by the Nile crocodiles increased slightly with increasing source distance. This was true whether a single-frequency (15 Hz or 40 Hz, relative distance error between 36 and 37%) or a multi-frequency (band width 1 - 80 Hz, relative distance error 25%) surface wave stimulus was offered. Even if the rewarded stimulus (40 Hz) was superimposed by an unrewarded surface wave some distance determination was observed (relative distance error between 30 and 62%)., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020. Published by Elsevier GmbH.)

Mechanoreception, the ability of robots to detect mechanical stimuli from the internal and external environments, contributes significantly to improving safety and task performance during the operation of robots in unstructured environments. Various approaches have been proposed to endow robot systems with mechanoreception. In the case of soft robots, the state-of-the-art mechanosensory solutions typically embedded dedicated deformable sensors into the soft body, giving rise to fabrication complexity and signal sophistication. In this study, we propose a novel mechanoreception scheme to enable pneumatic-driven soft robots to perceive proprioceptive movements as well as external contacts. Both internal and external mechanical parameters can be decoded from intuitive cues of body deformation and pneumatic pressure signals. In contrast to most existing solutions employing dedicated deformable sensors, the proposed approach only utilizes pressure feedback, which is typically available from the pneumatic pressure sensors incorporated in the control loop of most pneumatic soft robots. The concept was implemented and validated on a proprietary robotic gripper with a linear soft pneumatic actuator, demonstrating the capability in simultaneous detection of actuator position and external contact forceAfter the proposed approach, the gripper can achieve both active and passive mechanosensation, with demonstrated experiments in grasping force estimation, contact loss detection, object stiffness identification, and contour measurements. This approach offers an alternative route to achieving excellent internal/environmental awareness without requiring dedicated sensing modalities.

Recent evidence indicates active roles for the cerebrospinal fluid (CSF) on body axis development and morphogenesis of the spine, implying CSF-contacting neurons (CSF-cNs) in the spinal cord. CSF-cNs project a ciliated apical extension into the central canal that is enriched in the channel PKD2L1 and enables the detection of spinal curvature in a directional manner. Dorsolateral CSF-cNs ipsilaterally respond to lateral bending although ventral CSF-cNs respond to longitudinal bending. Historically, the implication of the Reissner fiber (RF), a long extracellular thread in the CSF, to CSF-cN sensory functions has remained a subject of debate. Here, we reveal, using electron microscopy in zebrafish larvae, that the RF is in close vicinity with cilia and microvilli of ventral and dorsolateral CSF-cNs. We investigate in vivo the role of cilia and the RF in the mechanosensory functions of CSF-cNs by combining calcium imaging with patch-clamp recordings. We show that disruption of cilia motility affects CSF-cN sensory responses to passive and active curvature of the spinal cord without affecting the Pkd2l1 channel activity. Because ciliary defects alter the formation of the RF, we investigated whether the RF contributes to CSF-cN mechanosensitivity in vivo. Using a hypomorphic mutation in the scospondin gene that forbids the aggregation of SCO-spondin into a fiber, we demonstrate in vivo that the RF per se is critical for CSF-cN mechanosensory function. Our study uncovers that neurons contacting the cerebrospinal fluid functionally interact with the RF to detect spinal curvature in the vertebrate spinal cord., Competing Interests: Declaration of Interests The authors declare no competing interests., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

MicroRNAs (miRNAs, miRs) are short noncoding RNAs that act to repress expression of proteins from target mRNA transcripts. miRNAs influence many cellular processes including stemness, proliferation, differentiation, maintenance, and survival, and miRNA mutations or misexpression are associated with a variety of disease states. The miR-183 family gene cluster including miR-183, miR-96, and miR-182 is highly conserved among vertebrate and invertebrate organisms, and the miRNAs are coordinately expressed with marked specificity in sensory neurons and sensory epithelial cells. The crucial functions of these miRNAs in normal cellular processes are not yet fully understood, but expectedly dependent upon the transcriptomes of specific cell types at different developmental stages or in various maintenance circumstances. This article provides an overview of evidence supporting roles for miR-183 family members in normal biology of the nervous system, including mechanoreception for auditory and vestibular function, electroreception, chemoreception, photoreception, circadian rhythms, sensory ganglia and pain, and memory formation.

Non-nervous auxiliary structures play a significant role in sensory biology. They filter the stimulus and transform it in a way that fits the animal's needs, thereby contributing to the avoidance of the central nervous system's overload with meaningless stimuli and a corresponding processing task. The present review deals with mechanoreceptors mainly of invertebrates and some remarkable recent findings stressing the role of mechanics as an important source of sensor adaptedness, outstanding performance, and diversity. Instead of organizing the review along the types of stimulus energy (force) taken up by the sensors, processes associated with a few basic and seemingly simple mechanical principles like lever systems, viscoelasticity, resonance, traveling waves, and impedance matching are taken as the guideline. As will be seen, nature makes surprisingly competent use of such "simple mechanics".

Vibrational communication is common in insects and often includes signals with prominent frequency components below 200 Hz, but the sensory adaptations for their detection are scarcely investigated. We performed an integrative study of the subgenual organ complex in Troglophilus cave crickets (Orthoptera: Rhaphidophoridae), a mechanosensory system of three scolopidial organs in the proximal tibia, for mechanical, anatomical and physiological aspects revealing matches to low frequency vibration detection. Microcomputed tomography shows that a part of the subgenual organ sensilla and especially the accessory organ posteriorly in this complex are placed closely underneath the cuticle, a position suited to evoke responses to low-frequency vibration via changes in the cuticular strain. Laser-Doppler vibrometry shows that in a narrow low-frequency range the posterior tibial surface reacts stronger to low frequency sinusoidal vibrations than the anterior tibial surface. This finding suggests that the posterior location of sensilla in tight connection to the cuticle, especially in the accessory organ, is adapted to improve detectability of low-frequency vibration signals. By electrophysiological recordings we identify a scolopidial receptor type tuned to 50-300 Hz vibrations, which projects into the central mechanosensory region specialised for processing low-frequency vibratory inputs, and most likely originates from the accessory organ or the posterior subgenual organ. Our findings contribute to understanding of the mechanical and neuronal basis of low-frequency vibration detection in insect legs and their highly differentiated sensory systems., (Copyright © 2019 Elsevier Inc. All rights reserved.)

The role of vision and the lateral-line system in fish-screen avoidance behaviours was investigated in shiner surfperch Cymatogaster aggregata and staghorn sculpin Leptocottus armatus. Avoidance experiments were conducted in front of water-diversion-type wedge-wire fish screens in a laboratory flume with a 0.3 m s -1 water velocity. Fish contacted the screens less frequently during the day than night, indicating that fish screen avoidance is visually mediated during the day. Input from the fishes' lateral-line systems was also blocked with streptomycin-sulphate treatments during the night to determine if these fishes use mechanoreceptive cues to guide screen avoidance in darkened conditions. Streptomycin-treated and untreated fish had similar contact rates suggesting that mechanoreceptive guidance was not increasing the fishes' abilities to avoid contact with non-vibrating screens at night. Fishes were stained with 2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide (DASPEI) to assess the streptomycin treatment's effectiveness. We also tested the fishes' ability to avoid contact with the screens at night, when a strobe light or industrial vibrator was operated on the screens, to respectively increase the screen's visual and mechanoreceptory guidance potential. Cymatogaster aggregata contacted the screens significantly less frequently when they were vibrating, compared with their night-time controls, suggesting useful mechanoreceptive guidance. Leptocottus armatus contacted the screens significantly less frequently under strobe-light illumination, compared with their night-time controls, suggesting useful visual guidance. This research should benefit fishery and water-resource managers, regarding the development of future fish-protection decisions at screened water diversions., (© 2018 The Fisheries Society of the British Isles.)

Hawkmoths (Lepidoptera, Sphingidae) comprise around 1500 species, most of which forage on nectar from flowers in their adult stage, usually while hovering in front of the flower. The majority of species have a nocturnal lifestyle and are important nocturnal pollinators, but some species have turned to a diurnal lifestyle. Hawkmoths use visual and olfactory cues including CO 2 and humidity to detect and recognise rewarding flowers; they find the nectary in the flowers by means of mechanoreceptors on the proboscis and vision, evaluate it with gustatory receptors on the proboscis, and control their hovering flight position using antennal mechanoreception and vision. Here, we review what is presently known about the sensory organs and sensory-guided behaviour that control feeding behaviour of this fascinating pollinator taxon. We also suggest that more experiments on hawkmoth behaviour in natural settings are needed to fully appreciate their sensory capabilities.

The reduced hindwings of flies, known as halteres, are specialized mechanosensory organs that detect body rotations during flight. Primary afferents of the haltere encode its oscillation frequency linearly over a wide bandwidth and with precise phase-dependent spiking. However, it is not currently known whether information from haltere primary afferent neurons is sent to higher brain centers where sensory information about body position could be used in decision making, or whether precise spike timing is useful beyond the peripheral circuits that drive wing movements. We show that in cells in the central brain, the timing and rates of neural spiking can be modulated by sensory input from experimental haltere movements (driven by a servomotor). Using multichannel extracellular recording in restrained flesh flies ( Sarcophaga bullata of both sexes), we examined responses of central complex cells to a range of haltere oscillation frequencies alone, and in combination with visual motion speeds and directions. Haltere-responsive units fell into multiple response classes, including those responding to any haltere motion and others with firing rates linearly related to the haltere frequency. Cells with multisensory responses showed higher firing rates than the sum of the unisensory responses at higher haltere frequencies. They also maintained visual properties, such as directional selectivity, while increasing response gain nonlinearly with haltere frequency. Although haltere inputs have been described extensively in the context of rapid locomotion control, we find haltere sensory information in a brain region known to be involved in slower, higher-order behaviors, such as navigation. SIGNIFICANCE STATEMENT Many animals use vision for navigation; however, these cues must be interpreted in the context of the body's position. In mammalian brains, hippocampal cells combine visual and vestibular information to encode head direction. A region of the arthropod brain, known as the central complex (CX), similarly encodes heading information, but it is unknown whether proprioceptive information is integrated here as well. We show that CX neurons respond to input from halteres, specialized proprioceptors in flies that detect body rotations. These neurons also respond to visual input, providing one of the few examples of multiple sensory modalities represented in individual CX cells. Haltere stimulation modifies neural responses to visual signals, providing a mechanism for integrating vision with proprioception., (Copyright © 2019 the authors.)

Mechanosensation provides animals with important sensory information in addition to olfaction and gustation during feeding behavior. Here, we used Drosophila melanogaster larvae to investigate the role of softness sensing in behavior and learning. In the natural environment, larvae need to dig into soft foods for feeding. Finding foods that are soft enough to dig into is likely to be essential for their survival. We report that larvae can discriminate between different agar concentrations and prefer softer agar. Interestingly, we show that larvae on a harder surface search for a softer surface using memory associated with an odor, and that they evaluate foods by balancing softness and sweetness. These findings suggest that larvae integrate mechanosensory information with chemosensory input while foraging. Moreover, we found that the larval preference for softness is affected by genetic background., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2019. Published by The Company of Biologists Ltd.)

In the coevolution of predator and prey, different and less well-understood rules for threat assessment apply to freely suspended organisms than to substrate-dwelling ones. Particularly vulnerable are small prey carried with the bulk movement of a surrounding fluid and thus deprived of sensory information within the bow waves of approaching predators. Some planktonic prey have solved this apparent problem, however. We quantified cues generated by the slow approach of larval clownfish ( Amphiprion ocellaris) that triggered a calanoid copepod ( Bestiolina similis) to escape before the fish could strike. To estimate water deformation around the copepod immediately preceding its jump, we represented the body of the fish as a rigid sphere in a hydrodynamic model that we parametrized with measurements of fish size, approach speed and distance to the copepod. Copepods of various developmental stages (CII-CVI) were sensitive to the water flow caused by the live predator, at deformation rates as low as 0.04 s -1 . This rate is far lower than that predicted from experiments that used artificial predator-mimics. Additionally, copepods localized the source, with 87% of escapes directed away (greater than or equal to 90°) from the predator. Thus, copepods' survival in life-threatening situations relied on their detection of small nonlinear signals within an environment of locally linear deformation.

Flying animals need continual sensory feedback about their body position and orientation for flight control. The visual system provides essential but slow feedback. In contrast, mechanosensory channels can provide feedback at much shorter timescales. How the contributions from these two senses are integrated remains an open question in most insect groups. In Diptera, fast mechanosensory feedback is provided by organs called halteres and is crucial for the control of rapid flight manoeuvres, while vision controls manoeuvres in lower temporal frequency bands. Here, we have investigated the visual-mechanosensory integration in the hawkmoth Macroglossum stellatarum. They represent a large group of insects that use Johnston's organs in their antennae to provide mechanosensory feedback on perturbations in body position. Our experiments show that antennal mechanosensory feedback specifically mediates fast flight manoeuvres, but not slow ones. Moreover, we did not observe compensatory interactions between antennal and visual feedback., Competing Interests: AD, AS, JF, SS, AK No competing interests declared, (© 2018, Dahake et al.)

Animals detect the force of gravity with multiple sensory organs, from subcutaneous receptors at body joints to specialized sensors like the vertebrate inner ear. The halteres of flies, specialized mechanoreceptive organs derived from hindwings, are known to detect body rotations during flight, and some groups of flies also oscillate their halteres while walking. The dynamics of halteres are such that they could act as gravity detectors for flies standing on substrates, but their utility during non-flight behaviors is not known. We observed the behaviors of intact and haltere-ablated flies during walking and during perturbations in which the acceleration due to gravity suddenly changed. We found that intact halteres are necessary for flies to maintain normal walking speeds on vertical surfaces and to respond to sudden changes in gravity. Our results suggest that halteres can serve multiple sensory purposes during different behaviors, expanding their role beyond their canonical use in flight., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2018. Published by The Company of Biologists Ltd.)

When one thinks of airborne organisms, spiders do not usually come to mind. However, these wingless arthropods have been found 4 km up in the sky [1], dispersing hundreds of kilometers [2]. To disperse, spiders "balloon," whereby they climb to the top of a prominence, let out silk, and float away. The prevailing view is that drag forces from light wind allow spiders to become airborne [3], yet ballooning mechanisms are not fully explained by current aerodynamic models [4, 5]. The global atmospheric electric circuit and the resulting atmospheric potential gradient (APG) [6] provide an additional force that has been proposed to explain ballooning [7]. Here, we test the hypothesis that electric fields (e-fields) commensurate with the APG can be detected by spiders and are sufficient to stimulate ballooning. We find that the presence of a vertical e-field elicits ballooning behavior and takeoff in spiders. We also investigate the mechanical response of putative sensory receivers in response to both e-field and air-flow stimuli, showing that spider mechanosensory hairs are mechanically activated by weak e-fields. Altogether, the evidence gathered reveals an electric driving force that is sufficient for ballooning. These results also suggest that the APG, as additional meteorological information, can reveal the auspicious time to engage in ballooning. We propose that atmospheric electricity adds key information to our understanding and predictive capability of the ecologically important mass migration patterns of arthropod fauna [8]. VIDEO ABSTRACT., (Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)

We investigate the influence of leg mechanics on the vibration input and function of vibrosensitive organs in the legs of the cave cricket Troglophilus neglectus, using laser Doppler vibrometry. By varying leg attachment, leg flexion, and body posture, we identify important influences on the amplitude and frequency parameters of transmitted vibrations. The legs respond best to relatively high-frequency vibration (200-2000 Hz), but in strong dependence on the leg position; the response peak shifts progressively over 500-1400 Hz towards higher frequencies following leg flexion. The response is amplified most strongly on the tibia, where specialised vibrosensory organs occur, and the response amplitude increases with the increasing frequency. Leg responses peaking at 800 and 1400 Hz closely resemble the tuning of the intermediate organ receptors in the proximal tibia of T. neglectus, which may be highly sensitive to positional change. The legs of free-standing animals with the abdomen touching the vibrating substrate show a secondary response peak below 150 Hz, induced by body vibration. Such responses may significantly increase the sensitivity of low-frequency receptors in the tibial accessory organ and the femoral chordotonal organ. The cave cricket legs appear suitable especially for detection of high-frequency vibration.

Advances in mechanobiology have evolved through insights from multiple disciplines including structural engineering, biomechanics, vascular biology, and orthopaedics. In this paper, we reviewed the impact of key reports related to the study of applied loads on tissues and cells and the resulting signal transduction pathways. We addressed how technology has helped advance the burgeoning field of mechanobiology (over 33,600 publications from 1970 to 2016). We analyzed the impact of critical ideas and then determined how these concepts influenced the mechanobiology field by looking at the citation frequency of these reports as well as tracking how the overall number of citations within the field changed over time. These data allowed us to understand how a key publication, idea, or technology guided or enabled the field. Initial observations of how forces acted on bone and soft tissues stimulated the development of computational solutions defining how forces affect tissue modeling and remodeling. Enabling technologies, such as cell and tissue stretching, compression, and shear stress devices, allowed more researchers to explore how deformation and fluid flow affect cells. Observation of the cell as a tensegrity structure and advanced methods to study genetic regulation in cells further advanced knowledge of specific mechanisms of mechanotransduction. The future of the field will involve developing gene and drug therapies to simulate or augment beneficial load regimens in patients and in mechanically conditioning organs for implantation. Here, we addressed a history of the field, but we limited our discussions to advances in musculoskeletal mechanobiology, primarily in bone, tendon, and ligament tissues. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:605-619, 2018., (© 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.)

Tactile-foraging ducks are specialist birds known for their touch-dependent feeding behavior. They use dabbling, straining, and filtering to find edible matter in murky water, relying on the sense of touch in their bill. Here, we present the molecular characterization of embryonic duck bill, which we show contains a high density of mechanosensory corpuscles innervated by functional rapidly adapting trigeminal afferents. In contrast to chicken, a visually foraging bird, the majority of duck trigeminal neurons are mechanoreceptors that express the Piezo2 ion channel and produce slowly inactivating mechano-current before hatching. Furthermore, duck neurons have a significantly reduced mechano-activation threshold and elevated mechano-current amplitude. Cloning and electrophysiological characterization of duck Piezo2 in a heterologous expression system shows that duck Piezo2 is functionally similar to the mouse ortholog but with prolonged inactivation kinetics, particularly at positive potentials. Knockdown of Piezo2 in duck trigeminal neurons attenuates mechano current with intermediate and slow inactivation kinetics. This suggests that Piezo2 is capable of contributing to a larger range of mechano-activated currents in duck trigeminal ganglia than in mouse trigeminal ganglia. Our results provide insights into the molecular basis of mechanotransduction in a tactile-specialist vertebrate., Competing Interests: The authors declare no conflict of interest.

Interactions of cells with supramolecular aggregates of the extracellular matrix (ECM) are mediated, in part, by cell surface receptors of the integrin family. These are important molecular components of cell surface-suprastructures regulating cellular activities in general. A subfamily of β1-integrins with von Willebrand-factor A-like domains (I-domains) in their α-chains can bind to collagen molecules and, therefore, are considered as important cellular mechano-receptors. Here we show that chondrocytes strongly bind to cartilage collagens in the form of individual triple helical molecules but very weakly to fibrils formed by the same molecules. We also find that chondrocyte integrins α1β1-, α2β1- and α10β1-integrins and their I-domains have the same characteristics. Nevertheless we find integrin binding to mechanically generated cartilage fibril fragments, which also comprise peripheral non-collagenous material. We conclude that cell adhesion results from binding of integrin-containing adhesion suprastructures to the non-collagenous fibril periphery but not to the collagenous fibril cores. The biological importance of the well-investigated recognition of collagen molecules by integrins is unknown. Possible scenarios may include fibrillogenesis, fibril degradation and/or phagocytosis, recruitment of cells to remodeling sites, or molecular signaling across cytoplasmic membranes. In these circumstances, collagen molecules may lack a fibrillar organization. However, other processes requiring robust biomechanical functions, such as fibril organization in tissues, cell division, adhesion, or migration, do not involve direct integrin-collagen interactions., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Bees and flowering plants have a long-standing and remarkable co-evolutionary history. Flowers and bees evolved traits that enable pollination, a process that is as important to plants as it is for pollinating insects. From the sensory ecological viewpoint, bee-flower interactions rely on senses such as vision, olfaction, humidity sensing, and touch. Recently, another sensory modality has been unveiled; the detection of the weak electrostatic field that arises between a flower and a bee. Here, we present our latest understanding of how these electric interactions arise and how they contribute to pollination and electroreception. Finite-element modelling and experimental evidence offer new insights into how these interactions are organised and how they can be further studied. Focussing on pollen transfer, we deconstruct some of the salient features of the three ingredients that enable electrostatic interactions, namely the atmospheric electric field, the capacity of bees to accumulate positive charge, and the propensity of plants to be relatively negatively charged. This article also aims at highlighting areas in need of further investigation, where more research is required to better understand the mechanisms of electrostatic interactions and aerial electroreception.

The forebrain somatic sensory locus for input from sensors on the surface of an active prosthesis is an important component of the Brain Machine Interface. We now review the neuronal responses to controlled cutaneous stimuli and the sensations produced by Threshold Stimulation at Microampere current levels (TMIS) in such a locus, the human thalamic Ventral Caudal nucleus (Vc). The responses of these neurons to tactile stimuli mirror those for the corresponding class of tactile mechanoreceptor fiber in the peripheral nerve, and TMIS can evoke sensations like those produced by the stimuli that optimally activate each class. These neuronal responses show a somatotopic arrangement from lateral to medial in the sequence: leg, arm, face and intraoral structures. TMIS evoked sensations show a much more detailed organization into anterior posteriorly oriented rods, approximately 300 microns diameter, that represent smaller parts of the body, such as parts of individual digits. Neurons responding to painful and thermal stimuli are most dense around the posterior inferior border of Vc, and TMIS evoked pain sensations occur in one of two patterns: (i) pain evoked regardless of the frequency or number of spikes in a burst of TMIS; and (ii) the description and intensity of the sensation changes with increasing frequencies and numbers. In patients with major injuries leading to loss of somatic sensory input, TMIS often evokes sensations in the representation of parts of the body with loss of sensory input, e.g., the phantom after amputation. Some patients with these injuries have ongoing pain and pain evoked by TMIS of the representation in those parts of the body. Therefore, thalamic TMIS may produce useful patterned somatotopic feedback to the CNS from sensors on an active prosthesis that is sometimes complicated by TMIS evoked pain in the representation of those parts of the body., Competing Interests: The authors declare no conflict of interest.

Sensory adaptation is characterized by a reduction in the firing frequency of neurons to prolonged stimulation, also called spike frequency adaptation. This has been documented for sensory neurons of the visual, olfactory, electrosensory, and auditory system both in response to constant-amplitude and to sinusoidal stimuli, but has thus far not been described systematically for the lateral line system. We recorded neuronal activity from primary afferent nerve fibres in the lateral line in goldfish in response to sinusoidal wave stimuli. Depending on stimulus characteristics, afferent fibre responses exhibited a distinct onset followed by a decline in firing rate to an apparent steady-state level, i.e., they exhibited adaptation. The degree of adaptation, measured as the percent decrease in firing rate between onset and steady-state, increased with stimulus amplitude and frequency and with increasing steepness of the rising flank of the stimulus. This may in part be due to the velocity and/or acceleration sensitivity of the lateral line receptors. The time course of the response decline, i.e., the time course of adaptation was best-fit by a power function. This is consistent with the previous studies on spike frequency adaptation in sensory afferents of weakly electric fish.

The size of the infraorbital foramen (IOF) is correlated with the size of the infraorbital nerve and number of mystacial vibrissae in mammals. Accordingly, IOF cross-sectional area has been used to infer both the rostral mechanoreceptive acuity and phylogenetic relationships of extinct crown primates and plesiadapiforms. Among living mammals, extant primates, scandentians, and dermopterans (Euarchonta) exhibit smaller IOF cross-sectional areas than most other mammals. Here we assess whether fossil adapoids, omomyoids, and plesiadapiforms show a reduction in relative IOF area similar to that characterizing extant euarchontans. The IOFs of 12 adapoid, 7 omomyoid, 15 plesiadapiform, and 3 fossil gliran species were measured and compared to a diverse extant mammalian sample. These data demonstrate that adapoids and omomyoids have IOFs that are similar in relative size to those of extant euarchontans. Conversely, IOFs of plesiadapiforms are on average about twice as large as those of extant euarchontans and are more comparable in size to those of extant non-euarchontan mammals. These results indicate that crown primates share a derived reduction in relative IOF size with treeshrews and colugos. Accordingly, a decreased reliance on the muzzle and an increased reliance on the hands for environmental exploration may have first evolved in the euarchontan stem lineage. However, the relatively large IOFs of plesiadapiforms imply a continued reliance on the muzzle for close exploration of objects. This finding may indicate that either parallel evolutionary decreases in IOF size occurred within Euarchonta or that plesiadapiforms lie outside the euarchontan crown group., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Although kissing bugs (Triatominae: Reduviidae) are perhaps best known as vectors of Chagas disease, they are important experimental models in studies of insect sensory physiology, pioneered by the seminal studies of Wigglesworth and Gillet more than eighty years ago. Since then, many investigations have revealed that the thermal, hygric, visual and olfactory senses play critical roles in the orientation of these blood-sucking insects towards hosts. Here we review the current knowledge about the role of these sensory systems, focussing on relevant stimuli, sensory structures, receptor physiology and the molecular players involved in the complex and cryptic behavioural repertoire of these nocturnal insects. Odours are particularly relevant, as they are involved in host search and are used for sexual, aggregation and alarm communication. Tastants are critical for a proper recognition of hosts, food and conspecifics. Heat and relative humidity mediate orientation towards hosts and are also important for the selection of resting places. Vision, which mediates negative phototaxis and flight dispersion, is also critical for modulating shelter use and mediating escape responses. The molecular bases underlying the detection of sensory stimuli started to be uncovered by means of functional genetics due to both the recent publication of the genome sequence of Rhodnius prolixus and the availability of modern genome editing techniques., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Presynaptic GABA B receptors (GABA B Rs) are highly expressed in dorsal root ganglion neurons and spinal cord dorsal horn. GABA B Rs located in superficial dorsal horn play an important antinociceptive role, by acting at both pre- and postsynaptic sites. GABA B Rs expressed in deep dorsal horn could be involved in the processing of touch sensation and possibly in the generation of tactile allodynia in chronic pain. The objective of this study was to characterize the morphological and functional properties of GABA B Rs expressed on Aβ fibers projecting to lamina III/IV and to understand their role in modulating excitatory synaptic transmission. We performed high-resolution electron microscopic analysis, showing that GABA B2 subunit is expressed on 71.9% of terminals in rat lamina III-IV. These terminals were engaged in axodendritic synapses and, for the 46%, also expressed glutamate immunoreactivity. Monosynaptic excitatory postsynaptic currents, evoked by Aβ fiber stimulation and recorded from lamina III/IV neurons in spinal cord slices, were strongly depressed by application of baclofen (0.1-2.5 µM), acting as a presynaptic modulator. Application of the GABA B R antagonist CGP 55845 caused, in a subpopulation of neurons, the potentiation of the first of two excitatory postsynaptic currents recorded with the paired-pulse protocol, showing that GABA B Rs are endogenously activated. A decrease in the paired-pulse ratio accompanied the effect of CGP 55845, implying the involvement of presynaptic GABA B Rs. CGP 55845 facilitated only the first excitatory postsynaptic current also during a train of four consecutive stimuli applied to Aβ fibers. These results suggest that GABA B Rs tonically inhibit glutamate release from Aβ fibers at a subset of synapses in deep dorsal horn. This modulation specifically affects only the early phase of synaptic excitation in lamina III-IV neurons.

Mechanosensitivity is a fundamental physiological capacity, which pertains to all life forms. Progress has been made with regard to understanding mechanosensitivity in bacteria, flies, and worms. In vertebrates, however, the molecular identity of mechanotransducers in somatic and neuronal cells has only started to appear. The Piezo family of mechanogated ion channels marks a pivotal milestone in understanding mechanosensitivity. Piezo1 and Piezo2 have now been shown to participate in a number of processes, ranging from arterial modeling to sensing muscle stretch. In this review, we focus on Piezo2 and its role in mediating mechanosensation and proprioception in vertebrates., (Copyright © 2017 Elsevier Inc. All rights reserved.)

For many animals processing of tactile information is a crucial task in behavioral contexts like exploration, foraging, and stimulus avoidance. The leech, having infrequent access to food, developed an energy efficient reaction to tactile stimuli, avoiding unnecessary muscle movements: The local bend behavior moves only a small part of the body wall away from an object touching the skin, while the rest of the animal remains stationary. Amazingly, the precision of this localized behavioral response is similar to the spatial discrimination threshold of the human fingertip, although the leech skin is innervated by an order of magnitude fewer mechanoreceptors and each midbody ganglion contains only 400 individually identified neurons in total. Prior studies suggested that this behavior is controlled by a three-layered feed-forward network, consisting of four mechanoreceptors (P cells), approximately 20 interneurons and 10 individually characterized motor neurons, all of which encode tactile stimulus location by overlapping, symmetrical tuning curves. Additionally, encoding of mechanical force was attributed to three types of mechanoreceptors reacting to distinct intensity ranges: T cells for touch, P cells for pressure, and N cells for strong, noxious skin stimulation. In this study, we provide evidences that tactile stimulus encoding in the leech is more complex than previously thought. Combined electrophysiological, anatomical, and voltage sensitive dye approaches indicate that P and T cells both play a major role in tactile information processing resulting in local bending. Our results indicate that tactile encoding neither relies on distinct force intensity ranges of different cell types, nor location encoding is restricted to spike count tuning. Instead, we propose that P and T cells form a mixed type population, which simultaneously employs temporal response features and spike counts for multiplexed encoding of touch location and force intensity. This hypothesis is supported by our finding that previously identified local bend interneurons receive input from both P and T cells. Some of these interneurons seem to integrate mechanoreceptor inputs, while others appear to use temporal response cues, presumably acting as coincidence detectors. Further voltage sensitive dye studies can test these hypotheses how a tiny nervous system performs highly precise stimulus processing.