1. Protective immunity against enteral stages of Trichinella spiralis elicited in mice by live attenuated Salmonella vaccine that secretes a 30-mer parasite epitope fused to the molecular adjuvant C3d-P28.
- Author
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Pompa-Mera EN, Arroyo-Matus P, Ocaña-Mondragón A, González-Bonilla CR, and Yépez-Mulia L
- Subjects
- Adjuvants, Immunologic chemistry, Amino Acid Sequence, Animals, Antibodies, Monoclonal immunology, Bacterial Vaccines chemistry, Epitopes chemistry, Male, Mice, Mice, Inbred BALB C, Trichinellosis immunology, Vaccines, Attenuated chemistry, Vaccines, Attenuated immunology, Adjuvants, Immunologic pharmacology, Bacterial Vaccines immunology, Epitopes immunology, Immunity drug effects, Trichinella spiralis immunology, Trichinellosis prevention & control
- Abstract
The development of a veterinary vaccine against T. spiralis infection is an alternative strategy to control trichinellosis. In an effort to develop an efficient vaccine, BALB/c mice were immunized with attenuated Salmonella enterica serovar Typhimurium SL3261 that expresses a 30-mer peptide (Ag30) derived from the gp43 of T. spiralis muscle larvae fused to three copies of the molecular adjuvant P28 (Ag30-P283) and it was either displayed on the surface or secreted by recombinant Salmonella strains. Salmonella strain secreting Ag30-P283, reduced the adult worm burden 92.8% following challenge with T. spiralis muscle larvae compared to 42% achieved by recombinant Salmonella displaying Ag30-P283 on the surface. The protection induced by secreted Ag30-P283 was associated with a mixed Th1/Th2 with predominance of Th2 phenotype, which was characterized by the production of IgG1, intestinal IgA antibodies and IL-5 secretion. This finding could provide an efficient platform technology for the design of novel vaccination strategies., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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