1. β-(1→3,1→6)-d-glucans produced by Diaporthe sp. endophytes: Purification, chemical characterization and antiproliferative activity against MCF-7 and HepG2-C3A cells.
- Author
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Orlandelli RC, Corradi da Silva ML, Vasconcelos AFD, Almeida IV, Vicentini VEP, Prieto A, Hernandez MDD, Azevedo JL, and Pamphile JA
- Subjects
- Antineoplastic Agents isolation & purification, Antineoplastic Agents pharmacology, Ascomycota chemistry, Carbohydrate Conformation, Cell Proliferation drug effects, Drug Screening Assays, Antitumor, Endophytes chemistry, Fungal Polysaccharides isolation & purification, Fungal Polysaccharides pharmacology, Hep G2 Cells, Humans, Inhibitory Concentration 50, MCF-7 Cells, Piper microbiology, Antineoplastic Agents chemistry, Fungal Polysaccharides chemistry
- Abstract
This study reports the characterization and antiproliferative activity of exopolysaccharides (EPS) produced by submerged cultures of the endophytes Diaporthe sp. JF766998 and Diaporthe sp. JF767007 isolated from the medicinal plant Piper hispidum Sw. Both strains secreted a crude EPS that, upon size exclusion chromatography, showed to contain a heteropolysaccharide (galactose, glucose and mannose) and a high-molecular weight glucan. Data from methylation analysis, FTIR and NMR spectroscopy (
1 H, COSY, TOCSY and HSQC-DEPT) indicated that the purified glucan consisted of a main chain of glucopyranosyl β-(1→3) linkages substituted at O-6 by glucosyl residues. According to MTT assay, some treatments of both β-glucans have antiproliferative activity against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells after 24 and 48h of treatment, exhibiting a degree of inhibition ratio that reached the highest values at 400μg/mL: 58.0% (24h) and 74.6% (48h) for MCF-7 cells, and 61.0% (24h) and 83.3% (48h) for HepG2-C3A cells. These results represent the first reports on the characterization and antiproliferative effect of β-glucans from Diaporthe species and also expand the knowledge about bioactive polysaccharides from endophytic sources., (Copyright © 2016 Elsevier B.V. All rights reserved.)- Published
- 2017
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