1. 25-hydroxyvitamin D-1alpha-hydroxylase in normal and malignant colon tissue.
- Author
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Tangpricha V, Flanagan JN, Whitlatch LW, Tseng CC, Chen TC, Holt PR, Lipkin MS, and Holick MF
- Subjects
- Calcium metabolism, Cause of Death, Cell Division genetics, Cell Transformation, Neoplastic pathology, Colon pathology, Colorectal Neoplasms mortality, Colorectal Neoplasms pathology, Gene Expression Regulation, Enzymologic physiology, Humans, Polymerase Chain Reaction, Reference Values, Sunlight, Survival Analysis, Vitamin D physiology, 25-Hydroxyvitamin D3 1-alpha-Hydroxylase genetics, Colorectal Neoplasms enzymology, RNA, Messenger genetics
- Abstract
Vitamin D affects calcium metabolism and prevents proliferation of colon cells in vitro. In human beings the main circulating form of vitamin D is 25-hydroxyvitamin D; to regulate calcium homoeostasis, this form must be converted to 1alpha, 25-dihydroxyvitamin D by 1alpha-hydroxylation in the kidney with 25-hydroxyvitamin D-1alpha-hydroxylase. Cultured transformed colon cancer cells can convert 25-hydroxyvitamin D(3) to 1alpha,25-dihydroxyvitamin D(3). We identified messenger RNA (mRNA) for 25-hydroxyvitamin D-1alpha-hydroxylase in normal colon tissue and in malignant and adjacent normal colon tissue. These findings support the notion that vitamin D might have a role in cell growth regulation and cancer protection, and might be the explanation for why the risk of dying from colorectal cancer is highest in areas with the least amount of sunlight.
- Published
- 2001
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