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Shale gas has been extensively extracted in the Sichuan Basin in China in recent years. To gain insight into the potential impact of shale gas wastewater (SGW) discharge, sediment in a small river receiving treated SGW, as well as cultivated soil and paddy soil irrigated by the river water were collected. The occurrence and distribution of polycyclic aromatic compounds (PACs), including polycyclic aromatic hydrocarbons (PAHs) and their alkylated/oxygenated derivatives (APAHs/OPAHs), and thiophenes were investigated, the resultant potential ecological risks were assessed subsequently. The total concentration of PACs varied in the range of 1299.9-9286.4, 2069.4-11,512.3, and 475.7-2927.9 ng/g in sediment, cultivated soil and paddy soil, respectively, with thiophenes followed by APAHs being the abundant components in all the studied samples, demonstrating the potential impact of SGW discharge on sediment and surrounding soil environment. Based on the measured concentrations, potential ecological risks posed by PAHs and APAHs were calculated, and moderate to high ecological risks were observed in partial sampling sites, which mainly caused by 3-4 rings PAHs and APAHs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are widely used across a spectrum of industrial and consumer goods. Nonetheless, their persistent nature and tendency to accumulate in biological systems pose substantial environmental and health threats. Consequently, striking a balance between maximizing product efficiency and minimizing environmental and health risks by tailoring the molecular structure of PFAS has become a pivotal challenge in the fields of environmental chemistry and sustainable development. To address this issue, a computational workflow was proposed for designing an environmentally friendly PFAS by incorporating deep learning (DL) and molecular generative models. The hybrid DL architecture MolHGT+ based on heterogeneous graph neural network with transformer-like attention was applied to predict the surface tension, bioaccumulation, and hepatotoxicity of the molecules. Through virtual screening of the PFAS master database using MolHGT+, the findings indicate that incorporating the siloxane group and betaine fragment can effectively decrease both the bioaccumulation and hepatotoxicity of PFAS while preserving low surface tension. In addition, molecular generative models were employed to create a structurally diverse pool of novel PFASs with the aforementioned hit molecules serving as the initial template structures. Overall, our study presents a promising AI-driven method for advancing the development of environmentally friendly PFAS., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Parkinson's disease (PD), a neurodegenerative disorder characterized by impaired motor function, is typically treated with medications and surgery. However, recent studies have validated physical exercise as an effective adjunct therapy, significantly improving both motor and non-motor symptoms in PD patients. Irisin, a myokine, has garnered increasing attention for its beneficial effects on the nervous system. Research has shown that irisin plays a crucial role in regulating metabolic balance, optimizing autophagy, maintaining mitochondrial quality, alleviating oxidative stress and neuroinflammation, and regulating cell death-all processes intricately linked to the pathogenesis of PD. This review examines the mechanisms through which irisin may counteract PD, provides insights into its biological effects, and considers its potential as a target for therapeutic strategies., Competing Interests: Declaration of competing interest There are no financial or personal relationships with any commercial entities or organizations that could be perceived as influencing the study's content, results, interpretation, or conclusions., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

It is a great challenge for effective treatment of shale gas produced water (SGPW), a typical industrial wastewater with complex composition. Single forward osmosis (FO) or membrane distillation (MD) process has been widely used for desalination of SGPW, with membrane fouling not well addressed. Fertilizer draw solution (DS) with high osmotic pressure is less likely to cause FO fouling and can be used for irrigation. An integrated process using fertilizer-driven FO (FDFO) and MD process was proposed for the first time for SGPW treatment, and characteristics of fertilizer DS and powdered activated carbon (PAC) enhancement were assessed. The DS using KCl and (NH 4 ) 2 SO 4 had high MD fluxes (36.8-38.8 L/(m 2 ·h)) and low permeate conductivity (below 50 μS/cm), increasing the contact angle of the MD membrane by 113 % than that without FO, while the DS using MgCl 2 and NH 4 H 2 PO 4 produced a lower reverse salt flux (0.9-3.2 g/(m 2 ·h)). When diluted DS was treated using PAC, the MD permeate conductivity was further reduced to 35 μS/cm without ammonia, and the membrane hydrophobicity was maintained to 71-83 % of the original. The mechanism of the FDFO-MD integrated process for mitigating MD fouling and improving permeate quality was analyzed, providing guidance for efficient SGPW treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Historical chlor-alkali production has led to substantial concentrations of persistent organic pollutant residues in the environment. This study systematically investigated the distribution of polycyclic aromatic hydrocarbons (PAHs), chlorinated/brominated-PAHs (Cl/Br-PAHs), polychlorinated naphthalenes (PCNs), and hexachlorobutadiene (HCBD) in sediment, lotus (Nelumbo nucifera), and fish samples from Ya-Er Lake, which is a site in China with historical chlor-alkali contamination. The average concentrations [(4.97-1.47) × 10 3  ng/g dry weight (dw)] of these pollutants in backfill sediments, which were dredged from the lake after chlor-alkali production stopped, were 2.68-70.87 times those in fresh lake sediments (0.622-218 ng/g dw) and reported concentrations in other areas. Correlation analyses indicated that Cl-PAHs, Br-PAHs, and PCNs likely originated from halogenation of parent PAHs in the study area, and the chlorination ratios were larger than those of bromination. The Cl (1/2/3) -PAHs/PAHs and Br (1) -PAHs/PAHs ratios were higher than those for PAHs with more halogen atoms. This contamination extended into the biota, with notable pollutant burdens found in lotus (Nelumbo nucifera, 0.305-77.3 ng/g dw) and even higher concentrations in fish (2.20-345 ng/g lipid weight). Estimated biological soil accumulation factors revealed significant enrichment in lotus organs (mean: 7.19) and fish muscle (mean: 10.65), especially the latter, which highlighted bioaccumulation and potential food chain transfer risks. The estimated daily intakes of PAHs, Cl/Br-PAHs, and HCBD through fish consumption currently pose negligible risks, while dietary intake of PCNs may present health concerns. Continuous monitoring and impact assessments are crucial for developing appropriate risk management strategies to safeguard public health., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The consistent presence of p-chloronitrobenzene (p-CNB) in groundwater has raised concerns regarding its potential harm. In this study, we developed a biocathode-anode cascade system in a permeable reactive barrier (BACP), integrating biological electrochemical system (BES) with permeable reactive barrier (PRB), to address the degradation of p-CNB in the groundwater. BACP efficiently accelerated the formation of biofilms on both the anode and cathode using the polar periodical reversal method, proving more conducive to biofilm development. Notably, BACP demonstrated a remarkable p-CNB removal efficiency of 94.76 % and a dechlorination efficiency of 64.22 % under a voltage of 0.5 V, surpassing the results achieved through traditional electrochemical and biological treatment processes. Cyclic voltammetric results highlighted the primary contributing factor as the synergistic effect between the bioanode and biocathode. It is speculated that this system primarily relies on bioelectrocatalytic reduction as the predominant process for p-CNB removal, followed by subsequent dechlorination. Furthermore, electrochemical and microbiological tests demonstrated that BACP exhibited optimal electron transfer efficiency and selective microbial enrichment ability under a voltage of 0.3-0.5 V. Additionally, we investigated the operational strategy for initiating BACP in engineering applications. The results showed that directly introducing BACP technology effectively enhanced microbial film formation and pollutant removal performance., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

E-waste recycling activities are a crucial emission source of organic pollutants, posing potential risks to the surrounding environment and human health. To understand the potential impact related to diverse e-waste dismantling activities, we investigated two categories of popular flame retardants (i.e., organophosphate esters (OPEs) and chlorinated paraffins (CPs) and their resultant possible ecological risk in 53 surface soil samples from Qingyuan, a well-known e-waste recycling region in South China. Varied concentrations of ΣOPEs (20.5-8720 ng/g) and ΣCPs (920-16800 ng/g) were observed at diverse dismantling sites, while relatively low levels of ΣOPEs (6.13-1240 ng/g) and ΣCPs (14.8-2870 ng/g) were found in surrounding soils. These results indicated that primitive e-waste dismantling processes were the primary emission source of OPEs and CPs in the studied area, with e-waste dumping and manual dismantling being the most important emission sources for OPEs and CPs. More importantly, CPs could be degraded/transformed into more toxic intermediates via dechlorination and decarbonization during the burning of e-waste. Furthermore, our results indicated the potential ecological risks posed by OPEs and CPs related to e-waste recycling., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Acrylamide is pervasive, and its exposure poses numerous health risks. This study examines both the direct and transgenerational effects of acrylamide toxicity in Caenorhabditis elegans, focusing on physiological and behavioral parameters. Parental exposure to acrylamide compromised several aspects of nematode health, including lifespan, reproductive capacity, body dimensions, and motor and sensory functions. Notably, while exposure to low concentrations of acrylamide did not alter the physiological traits of the offspring-except for their learning and memory-these findings suggest a possible adaptive response to low-level exposure that could be inherited by subsequent generations. Furthermore, continued acrylamide exposure in the offspring intensified both physiological and perceptual toxicity. Detailed analysis revealed dose-dependent alterations in acrylamide's detoxification and metabolic pathways. In particular, it inhibits the gene gst-4, which encodes a crucial enzyme in detoxification, mitigates DNA damage induced by acrylamide, and highlights a potential therapeutic target to reduce its deleterious effects., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Tris(2-chloroethyl) phosphate (TCEP) is of growing public concern worldwide because of its ubiquitous contamination, toxicity, and persistence. In this study, we investigated bacterial communities in aerobic enrichment cultures with TCEP and its two major transformation products bis(2-chloroethyl) phosphate (BCEP) and 2-chloroethanol (2-CE) as the sole carbon source. Burkholderiales and Rhizobiales were likely two main bacterial guilds involved in the hydrolysis of TCEP, while Rhizobiales and Sphingomonadales may play an important role in the hydrolysis of BCEP, given the increase of Rhizobiales and Sphingomonadales-related phosphoesterase genes when the carbon source was switched from TCEP to BCEP. All Burkholderiales, Rhizobiales, Sphingomonadales were probably efficient in 2-CE metabolism, because their dehydrogenase genes and dehalogenase genes increased after 2-CE enrichment. The different substrate preference of different bacterial guilds highlighted the importance to understand the diversity and collaboration among functional bacteria. Meanwhile, two TCEP-degrading strains affiliated with Xanthobacter and Ancylobacter were isolated. Xanthobacter sp. strain T2-1 was able to degrade both TCEP and BCEP following the pseudo-first-order kinetics with reaction rates of 1.66 h -1 for TCEP and 1.02 h -1 for BCEP, respectively. Ancylobacter sp. strain T3-4 could degrade TCEP following the pseudo-first-order kinetics with a reaction rate of 2.54 h -1 , but is unable to degrade BCEP. Additionally, strains that were phylogenetically closely related were found to have different degradation capabilities toward TCEP and/or BCEP, indicating the importance to investigate functional genes such as phosphoesterase genes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Background: Diallyl trisulfide (DATS) has a direct antioxidant capacity and emerges as a promising neuroprotective agent. This study was designed to investigate the role of DATS in traumatic brain injury (TBI)., Methods: TBI mouse models were established using the controlled cortical impact, followed by DATS administration. The effects of DATS on neurological deficit, brain damage, inflammation and phosphoglycerate kinase 1 (PGK1) expression were detected using mNSS test, histological analysis, TUNEL assay, enzyme-linked immunosorbent assay and immunofluorescence. PC12 cells were subjected to H 2 O 2 -induced oxidative injury after pre-treatment with DATS, followed by cell counting kit-8 assay, flow cytometry and ROS production detection. Apoptosis-related proteins and the PGK1/nuclear factor erythroid-2 related factor 2 (Nrf2) pathway were examined using Western blot., Results: DATS ameliorated the cerebral cortex damage, neurological dysfunction and apoptosis, as well as decreased PGK1 expression and expressions of pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) in mice after TBI. DATS also enhanced viability, blocked apoptosis and inhibited ROS production in H 2 O 2 -induced PC12 cells. DATS downregulated Cleaved-Caspase3, Bax and PGK1 levels, and upregulated Bcl-2 and Nrf2 levels in TBI mouse models and the injured cells., Conclusion: DATS regulates PGK1/Nrf2 expression and inflammation to alleviate neurological damage in mice after TBI., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The reduction in lung cancer mortality rates over the past decade can be partially ascribed to advancements in immunotherapy. Immune checkpoint inhibitors (ICIs) have transformed the therapeutic landscape for advanced non-small cell lung cancer (NSCLC) and have recently been evaluated in multiple clinical trials to confirm their safety and efficacy in the neoadjuvant, adjuvant and perioperative settings for patients with resectable NSCLC. The Food and Drug Administration (FDA) has granted approval for adjuvant atezolizumab following platinum-doublet chemotherapy, neoadjuvant nivolumab and platinum-doublet chemotherapy, adjuvant pembrolizumab after platinum-doublet chemotherapy, and neoadjuvant/adjuvant pembrolizumab for resectable NSCLC, with potential forthcoming approvals for additional agents or indications. Novel data, approvals, and emerging research findings are dramatically shifting the accepted standards of care over just a few years. Despite these advances, the optimal application of these treatments is not entirely straightforward. This article summarizes the biological rationale for immunotherapy and the important clinical trials regarding perioperative ICIs. We also further outline the controversies and future directions to better guide the individualized treatment of NSCLC patients., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Alien invasive aquatic-plant (AIA) species are severely threatening the aquatic ecosystems worldwide, especially biodiversity. Although plankton have been used to monitor and address biodiversity, some gaps remain in understanding of the relationships between plankton communities and AIA species. Here, the effects of two typical AIA species (Pistia stratiotes and Eichhornia crassipes) on plankton communities in freshwater with a native plant Vallisneria natans were investigated using a 50-d microcosm experiment. Results showed that AIA species significantly decreased water pH and dissolved oxygen while increased oxidation-reduction potential (p < 0.05). AIA species, especially P. stratiotes, significantly inhibited dry biomass accumulation in V. natans by an average rate of 39.0 %, decreased water pH by up to 14.62 %, and increased aboveground lengths and chlorophyll contents of V. natans by up to 36.2 % and 63.7 % (p < 0.05), respectively. These species further modified the growth strategy of V. natans from dry biomass accumulation to aboveground elongation. Although the AIA species did not alter plankton diversity (p > 0.05), but they changed their dominant species, functional communities (e.g., Groups D and T B ), and co-occurrence networks. P. stratiotes decreased the average degree of the networks by 12.37-19.02 % and the graph density by 10.53-14.47 %, while E. crassipes decreased the modularity of the networks by 10.24 % compared with the control (without AIA species), respectively. Overall, AIA species inhibited the growth of V. natans and decreased the stability of plankton communities and their resistance to environmental disturbances. These findings enhance our understanding of how AIA species affect the growth of native plants and variations in plankton communities, thereby providing a theoretical basis for improving the ecological function and safety of freshwater., Competing Interests: Declaration of competing interest The authors declare that they have no conflicts of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Renal cell carcinoma (RCC) represents a significant portion of genitourinary cancers, marked by challenging prognosis and high metastasis rates. Immunotherapy has been applied in managing advanced renal cell carcinoma, but the therapeutic outcomes are unsatisfactory. In this study, we order to construct a Janus kinase/signal transduction and activator transcriptional (JAK/STAT)-related signature linked to kidney patient outcomes for better predicting the efficacy to immune checkpoint inhibitors (ICIs) and to provide guidance for effective combination therapy. We screened 25 differentially expressed genes (DEGs) that exhibited high expression in RCC samples and were enriched in the JAK-STAT signaling pathway. Among these genes, 11 key genes were identified and correlated with the expectation of Kidney Clear Cell Carcinoma (KIRC) patients and all these genes was significantly elevated in RCC tumor tissues and cancer cells compared to para-cancer tissues and normal renal cells. Utilizing these 11 genes, we divided RCC patients into high-risk and low-risk groups. We found a clear correlation between the clinicopathologic factors of KIRC patients and the JAK-STAT-related risk score. And the IHC results shown that the JAK3 and STAT4 expression of tumor was significantly higher than normal tissue in RCC patients, the level of JAK3 and STAT4 was positively related to the T stage of RCC patients. In addition, high-risk patients had a poorer prognosis and greater protumor immune cell infiltration, and benefitted less from immunotherapy than did low-risk patients. Furthermore, the JAK-STAT-related risk score can predict disease-free survival (DFS) in RCC patients according to the nomogram, which constructed in combination with other clinical features such as age, TNM-staging and stage. Our study demonstrated the JAK-STAT signaling pathway's important regulatory function in RCC tumor immunity. This insight not only enhances our ability to accurately predict the survival rate of RCC patients, but also underscores a potential therapeutic alternative for RCC, involving the combined targeting of the JAK-STAT pathway and immune checkpoints., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Pulmonary tuberculosis and lung cancer are both common clinical diseases and serious public health problems. These two diseases can form a comorbidity by promoting each other under the influence of multiple factors during their occurrence and development. In recent years, some progress has been made in understanding the mechanism and exploring the diagnosis and treatment of comorbidity. However, there are still some unclear mechanisms that require further investigation. This paper briefly introduces the epidemiological characteristics of the comorbidity between pulmonary tuberculosis and lung cancer, and focuses on the potential pathogenesis of the comorbidity. It highlights the current challenges and looks ahead to potential future research directions, including suggestions for the development of new diagnostic and therapeutic strategies for the comorbidity.

Parkinson's disease (PD) is the second most common neurodegenerative disorder whose etiology remains unknown. The immune system has been implicated in hallmarks of PD including aggregation of α-synuclein and death of dopaminergic neurons in the substantia nigra. As a core regulator of immune response and inflammation, liver X receptors (LXRs) have been shown to have protective effects in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD. With two isoforms of LXRs (LXRα and LXRβ) expressed in the brain, their roles and distributions in this tissue remain largely unexplored. Here, we used MPTP to mimic symptoms and biomedical changes seen in PD in LXRα -/- and wild-type mice to investigate the role of LXRα in the etiology and progression of PD. We found that MPTP is unable to induce motor deficits, anxiety-like behavior in LXRα -/- mice, which has been seen in WT mice. Gene ontology analysis of RNA sequencing revealed that knockout of LXRα led to enrichment of the process, including immune response and inflammation in the midbrain. In addition, MPTP did not lead to dopaminergic neuron death in the striatum and substantia nigra in LXRα -/- mice, the basal GFAP protein level, and pro-inflammatory cytokines were elevated in LXRα -/- mice. Lastly, the microglia activation and astrogliosis caused by MPTP intoxication we found in WT mice were abolished in LXRα -/- mice. To sum up, we conclude that LXRα is a critical regulator in MPTP intoxication and may play a unique role in astrogliosis seen in the neuroinflammation of PD., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 International Brain Research Organization (IBRO). Published by Elsevier Inc. All rights reserved.)

Secondary organic aerosol (SOA) accounts for a large fraction of fine particulate matter (PM 2.5 ), but the lack of vertical observations of SOA in the urban boundary layer (UBL) limits a comprehensive understanding of its sources and formation mechanisms. In this study, PM 2.5 samples were simultaneously collected at 3 m, 118 m, and 488 m on the Canton Tower in Guangzhou during winter. Typical SOA tracers, including oxidation products of isoprene (SOA I ), monoterpene (SOA M ), sesquiterpene (SOA S ), and toluene (ASOA), were investigated alongside meteorological parameters and gaseous/particulate pollutants. Total concentrations of SOA tracers showed an increasing trend with height, with daytime levels exceeding nighttime levels. C 5 -alkene triols and 2-methylglyceric acid displayed a significant increase with height, potentially affected by nighttime chemistry in the residual layer, determining the overall vertical trend of SOA I tracers. Concentrations of later-generation SOA M (SOA M&#95;S ) tracers also increased with height, while those of first-generation SOA M (SOA M&#95;F ) tracers decreased, indicating relatively aged SOA M in the upper layers. SOA S and ASOA tracers exhibited higher enhancement under polluted conditions, likely impacted by biomass burning and anthropogenic emissions. The yields of SOA I tracers varied with temperature in the vertical profile. The formation of SOA M&#95;F tracers was negatively correlated with relative humidity, liquid water content, and pH, affecting their vertical distributions. The effect of O 3 on SOA formation enhanced significantly with height, influenced by air mass transport, and likely contributed to the higher yields of SOA in the upper layer. However, at ground level, SOA formation was primarily driven by high local emissions of both NO x and volatile organic compounds. We also observed the roles of SO 2 in SOA generation, particularly at 118 m. This study demonstrates the vertical diurnal characteristics of SOA tracers in the UBL, highlighting the varying effects of meteorological conditions and anthropogenic pollutants on SOA formation at different heights., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Peptide-based biofluorescents are of great interest due to their controllability and biocompatibility, as well as their potential applications in biomedical imaging and biosensing. Here, we present a simple approach to synthesizing full-color fluorescent nanomaterials with broad-spectrum fluorescence emissions, high optical stability, and long fluorescence lifetimes. By doping amino acids during the enzyme-catalyzed oxidative self-assembly of tyrosine-based peptides, we can precisely control the intermolecular interactions to obtain nanoparticles with fluorescence emission at different wavelengths. The synthesized peptide-based fluorescent nanomaterials with excellent biocompatibility and stable near-infrared fluorescence emission were shown to have potential for bioimaging applications. This research provides new ideas for the development of new bioluminescent materials that are cost-effective, environmentally friendly, and safe for biomedical use.

There is a long-standing debate over the effectiveness of chemical extraction methods in assessing soil metal phytoavailability. This study addresses the limitations of widely-used chemical extraction methods and presents the water-extractable pool as a more reliable indicator based on wheat pot experiments using homogenized agricultural soil amended with lime materials, phosphate, and biochar. Over 120 days' pot experiments, Cd accumulation in whole wheat plants and tissues exhibited positive relationships with water-extractable Cd concentrations at heading and maturity stage (Spearman's rho: 0.521-0.851; P < 0.05), revealing that the water-extractable pool instead of other pools better indicates wheat metal accumulation. Water-extractable metal concentrations are effective in assessing phytoavailability of metals primarily in ionic forms in soil solution (e.g, Zn, Cd), but less reliable for metals strongly complexed with dissolved organic matter (DOM) or sensitive to redox conditions. It demonstrated that water-extractable metal concentrations and chemical forms are key factors, fundamentally determined by metal properties and impacted by environmental factors. This study clarifies a more direct link between chemical extraction and plant metal uptake mechanisms. Given the extensive application of chemical extraction methods over several decades, this study will help advance soil metal risk assessment and remediation practices., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The study of antibiotic resistance in the silage microbiome has attracted initial attention. However, the influences of lactic acid bacteria inoculants and dry matter (DM) content on antibiotic resistance genes (ARGs) reduction in whole-plant corn silage remain poorly studied. This study accessed the ARGs' risk and transmission mechanism in whole-plant corn silage with different DM levels and treated with Lactiplantibacillus plantarum or Lentilactobacillus buchneri. The macrolide and tetracycline were the main ARGs in corn silage. The dominant species (Lent. buchneri and Lactobacillus acetotolerans) were the main ARGs carriers in whole-plant corn silage. The application of Lent. buchneri increased total ARGs abundance regardless of corn DM. Whole-plant corn silage with 30 % DM reduced the abundances of integrase and plasmid compared with 40 % DM. The correlation and structural equation model analysis demonstrated that bacterial community succession, resulting from changes in DM content, was the primary driving factor influencing the ARGs distribution in whole-plant corn silage. Interestingly, whole-plant corn silage inoculated with Lent. buchneri reduced abundances of high-risk ARGs (mdtG, mepA, tetM, mecA, vatE and tetW) by regulating pathogens (Escherichia coli), mobile genetic elements (MGEs) genes (IS3 and IS1182), and this effect was more pronounced at 30 % DM level. In summary, although whole-plant corn silage inoculated with Lent. buchneri increased the total ARGs abundance at both DM levels, it decreased the abundance of high-risk ARGs by reducing the abundances of the pathogens and MGEs, and this effect was more noticeable at 30 % DM level., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing for financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Landfill leachate treatment plants (LLTPs) harbor a vast reservoir of uncultured microbes, yet limited studies have systematically unraveled their functional potentials within LLTPs. Combining 36 metagenomic and 18 metatranscriptomic datasets from a full-scale LLTP, we unveiled a double-edged sword role of unknown species in leachate biotreatment and environmental implication. We identified 655 species-level genome bins (SGBs) spanning 47 bacterial and 3 archaeal phyla, with 75.9 % unassigned to any known species. Over 90 % of up-regulated functional genes in biotreatment units, compared to the leachate influent, were carried by unknown species and actively participated in carbon, nitrogen, and sulfur cycles. Approximately 79 % of the 37,366 carbohydrate active enzymes (CAZymes), with ∼90 % novelty and high expression, were encoded by unknown species, exhibiting great potential in biodegrading carbohydrate compounds linked to human meat-rich diets. Unknown species offered a valuable genetic resource of thousands of versatile, abundant, and actively expressed metabolic gene clusters (MGCs) and biosynthetic gene clusters (BGCs) for enhancing leachate treatment. However, unknown species may contribute to the emission of hazardous N 2 O/H 2 S and represented significant reservoirs for antibiotic-resistant pathogens that posed environmental safety risks. This study highlighted the significance of considering both positive and adverse effects of LLTP microbes to optimize LLTP performance., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Declaration of Competing Interest The authors declare no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Depleted uranium (DU) from corroded armor penetrators can migrate through the soil vadose zone and cause environmental problems, yet studies on such migration at former theatres of war are scarce. Here, we investigated vertical DU migration in a soil profile due to a penetrator (3-8 cm beneath the soil surface) corroded over 7 years in Bosnia and Herzegovina. The highest concentration of DU was ∼45,300 mg/kg at 6-10 cm, with the concentration decreasing markedly with increasing depth. The majority of the DU accumulated within the top 20 cm and the DU front reached ∼42 cm beneath the penetrator. In addition, particles with varying U concentrations (3-65 wt%) were observed at 0-15 cm, with U primarily co-located with O, Si, Al, maghemite, and hematite. Particularly, metaschoepite was identified at 6-10 cm. Finally, X-ray absorption spectroscopy analysis found U was hexavalent in the soil profile. These findings suggest that the downward migration of DU was likely present as a soluble form adsorbed on clay minerals and Fe oxides. Overall, we show that the rate of DU migration within the vadose zone is comparatively slow, although if the penetrator is left in the soil for decades, it could pose a serious long-term risk. ENVIRONMENTAL IMPLICATIONS: Over 90 % of the depleted uranium (DU) penetrators fired in previous conflicts missed their armored targets and were left in the soil to corrode. The corroded penetrators can not only contaminate soil but also pose a risk to groundwater. The present study examined the migration of DU in a soil profile that included a DU penetrator that had been corroding for over 7 years. Studying the dynamics of DU migration is essential to develop effective remediation strategies to mitigate long-term environmental risks and safeguard ecosystems and human health from DU contamination., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Bisphenol A (BPA), a known endocrine disruptor, is ubiquitous in various aquatic environments. Appendicularians are among the most abundant mesozooplankton populations and occupy a crucial niche in marine ecosystems. However, no toxicological data are available concerning the effects of BPA on this functional group. In this study, an evaluation of the toxicity of environmentally relevant levels of BPA (2.5-150 μg/L) on the appendicularian Oikopleura dioica, including its morphology and transcriptome, was conducted. Our results demonstrated the high sensitivity of O. dioica to BPA, with a LC 50 of 142 μg/L. Exposure to 125 μg/L BPA significantly inhibited the somatic growth, gonadal development and reproduction of individuals, whereas exposure to an environmentally safe concentration (2.5 μg/L) affected female fecundity and fitness as well as male gene expression. The results of the transcriptomic analysis suggest that males were more sensitive to BPA stress at the molecular level. BPA exposure not only led to abnormal secretion of digestive enzymes and phospholipase A2, affecting the function of the digestive system and arachidonic acid but also significantly down-regulated the expression of mRNAs related to enzymes involved in carbohydrate and energy metabolism in males. These findings suggest that the current safe environmental concentrations may not be safe., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Shuai Li reports financial support was provided by National Natural Science Foundation of China. Yi Liang reports financial support was provided by Shandong Provincial Natural Science Foundation. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Owing to the long-lived decay of triplet excited state, extensive efforts have been devoted to efficient triplet generation for applications covering triplet-triplet annihilation for photon upconversion, photocycloaddition and photoredox catalysis. Among the candidates, nanocrystal-molecule complexes have received tremendous attention for triplet generation because of easier spin flip and negligible energy loss during intersystem crossing. However, the triplet energy transfer (TET) from nanocrystals (NCs) to molecules can be very complicated in actual situation due to intricate energy level alignment and inevitable defect states, which often involves various decay pathes of the excited state competing with TET. Understanding the detailed carrier dynamics in such complexes is strongly necessary for related applications. Here, a CdSe-TCA (5-tetracene carboxylic acid) complex with a Type-II like energy level alignment is synthesized through precisely adjusting the dimension of CdSe NC. Based on series of spectral measurements, especially the transient absorption (TA) spectroscopy, the results show various carrier dynamics including hole-transfer-mediated TET, Förster resonance energy transfer (FRET) and carrier trapping. Although the carrier trapping by defect states in CdSe NC is revealed not associated with the TET from CdSe to TCA, the FRET is proved to competing with the TET process. Both the FRET and defect states should be refrained for efficient TET in such complexes. This study could provide further insight for understanding the carrier dynamics competition in NC-molecule complexes for triplet generation and benefit related optoelectronics applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Five series of cyanostilbene-based rod-like liquid crystals containing one different terminal atom (H, F, Cl, Br and I) at one end and one terminal aliphatic chain with different numbers of carbon atoms at the other end were reported by Suzuki coupling and Knoevenagel reactions. The influence of terminal halogen atoms and terminal chain length on the self-assembly, AIE behavior, temperature-dependent emission and mechanochromism behavior was explored by POM, DSC, XRD, SEM, absorption spectra and emission spectra. All the compounds are enantiotropic liquid crystals. The lowest non-halogen substituted homologue exhibited solo N phase, but the higher non-halogen substituted homologues exhibited mesogenic transition from SmA phase to N phase upon rising temperature. All the lowest halogen substituted homologues exhibited mesogenic transition from SmA phase to N phase upon rising temperature and all the higher homologues only exhibited SmA. The distinct mesogenic phase transition could be attributed to the intermolecular interaction produced by terminal halogen and the rigidity of the terminal aliphatic chain. All the non-halogen substituted compounds and halogen substituted compounds with smaller terminal halogen atom (F, Cl and Br atom) exhibited AIE behaviors, whereas the iodinated compounds exhibited extremely weak emission in solution and aggregated states due to the heavy atoms effect. These compounds also exhibited distinct solid-state emission with blue or cyan fluorescence, which could be quenched by increasing temperature. The reversible mechanochromism behavior was also achieved in all the compounds. The mechanical force induced quench in emission in non-halogen substituted compounds and halogen substituted compounds with smaller terminal halogen atom (F, Cl and Br atom), whereas enhancement in iodinated compounds. The reversible mechanochromism behavior endowed these compounds with potential applications in rewritable paper and anti-counterfeiting. The interesting properties in these liquid crystals would be attributed to the balance of the halogen-halogen interactions, heavy atom effect, steric-hindrance effect and chain length. These investigations would be helpful to understand the relationship between chemical structures and properties., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Alpha oscillations are associated with various psychiatric disorders, with many studies focusing on the prefrontal cortex, where transcranial alternating current stimulation (TACS) is applied in the alpha frequency band. This approach often involves selecting individualized alpha frequencies to resonate with their endogenous alpha oscillations. While strong alpha oscillations (8-13 Hz) are typically induced when the eyes are closed, they can also occur during the resting state with eyes open. However, it remains unclear whether these alpha oscillations share a common neural generation mechanism. Exploring which of these alpha oscillations is more suitable as a stable alpha peak frequency is a question of significant interest. Therefore, to systematically study this issue, we specifically collected resting-state electroencephalographic (EEG) data from the prefrontal region of 40 individuals, under both eyes-open and closed- eye conditions, with multiple follow-ups extending up to nine months. Through spectral analysis on each person's entire dataset and averaging the results, we observed a significant positive correlation between the alpha-band power in the eyes-open and the eyes-closed states, in terms of both absolute power and relative power. Further analysis revealed that this correlation was primarily contributed by the periodic activity within the alpha band. Upon modelling the oscillatory components, we discovered distinct differences in the oscillatory characteristics-such as number of the alpha sub-oscillations between the eyes-open state and the eyes-closed state. Our study is the first to systematically explored the relationship between alpha oscillations in the prefrontal cortex in the eyes-open and eyes-closed states, identifying both shared part of the neural generation mechanism and some distinct neural mechanisms that are unique to each state., (© 2024. The Author(s).)

Introduction: Radiotherapy-related damage of normal tissue inevitably influences the treatment outcomes in the context of high-grade gliomas (HGGs) treatment. We reported the survival outcomes and toxicities of patients with HGG treated with helical tomotherapy (HT) and the prognostic factors were analyzed., Materials and Methods: A total of 67 patients (29 had grade III and 38 had grade IV HGGs) who received HT between January 2016 and June 2020 were analyzed. Overall survival (OS) and progression-free survival (PFS) from the beginning of HT and OS from surgery were assessed, and toxicity and disease control were described briefly., Results: For patients with grade III HGGs, median OS (mOS) and median PFS (mPFS) from the beginning of HT were 68.933 and 62.967 months, respectively. For patients with grade IV HGGs, mOS and mPFS from the beginning of HT were 19.667 and 7.23 months, respectively. No grade ≥3 acute or late nonhematologic toxicities were observed. Multivariable Cox regression analysis showed that methylguanine methyltransferase (MGMT) methylated status, age, number of lesions, WHO grade, and monocyte count for PFS were significant. Age, monocyte count, and isocitrate dehydrogenase (IDH) status for OS., Conclusion: Treatment of HGGs with HT appears to be potentially effective and safe. HT is promising for glioblastomas (GBM), especially complex cases with infratentorial involvement or multiple lesions. This study highlighted the potential clinical significance of systemic inflammation indicators in predicting survival and disease progression.

C-H bond functionalization involving C,C-palladacycle intermediates provides a unique platform for developing novel reactions. However, the vast majority of studies have been limited to the transformations of C(aryl),C-palladacycles. In sharp contrast, catalytic reactions involving C(alkyl),C(alkyl)-palladacycles have rarely been reported. Herein, we disclose an unprecedented cascade C(sp 3 )-H annulation involving C(alkyl),C(alkyl)-palladacycles. In this protocol, alkene-tethered cycloalkenyl bromides undergo intramolecular Heck/C(sp 3 )-H activation to generate C(alkyl),C(alkyl)-palladacycles, which can be captured by α-bromoacrylic acids to afford tricyclic fused pyridinediones. In addition, this strategy can also be applied to indole-tethered cycloalkenyl bromides to construct pentacyclic fused pyridinediones via suquential Heck dearomatization/C(sp 3 )-H activation/decarboxylative cyclization. Notably, the removal of α-bromoacrylic acids in the reaction of alkene-tethered cycloalkenyl bromides can build an interesting tricyclic skeleton containing a four-membered ring. Preliminary mechanistic experiments indicate that five-membered C(alkyl),C(alkyl)-palladacycles serve as the key intermediates. Meanwhile, density functional theory (DFT) calculations have provided insights into the reaction pathway., (© 2024 Wiley-VCH GmbH.)

Charge stripes have been widely observed in many different types of unconventional superconductors, holding varying periods ( P ) and intensities. However, a general understanding on the interplay between charge stripes and superconducting properties is still incomplete. Here, using large-scale unbiased numerical simulations on a general inhomogeneous Hubbard model, we discover that the charge-stripe period P , which is variable in different real material systems, could dictate the pairing symmetries-d wave for P ≥ 4 , s and d waves for P ≤ 3 . In the latter, tuning hole doping and charge-stripe amplitude can trigger a d-s wave transition and magnetic-correlation shift, where the d-wave state converts to a pairing-density wave state, competing with the s wave. These interesting phenomena arise from an unusual stripe-induced selection rule of pairing symmetries around on-stripe region and within inter-stripe region, giving rise to a critical point of P = 3 for the phase transition. In general, our findings offer important insights into the differences in the superconducting pairing mechanisms across many P -dependent superconducting systems, highlighting the decisive role of charge stripe., (© 2024. The Author(s).)

To effectively mitigate carbon emissions and promote sustainability in the polymer field, biological macromolecules have emerged as a prominent strategy for fabricating functional materials. Herein, tannin (TA) was used as a biological curing agent to design fully bio-based polylactic/epoxidized natural rubber thermoplastic vulcanizates (PLA/ENR TPVs) with mechanical robustness and multi-stimuli-responsive shape memory properties. A dual cross-linking network, comprising both covalent bonds and hydrogen bonds, was successfully constructed in the ENR phase. A special co-continuous morphology was concomitantly constructed in the TPVs, which promoted effective stress transfer between the PLA and ENR phases, endowing the TPVs with balanced stiffness-toughness and shape memory properties. Moreover, the photothermal effect of TA also made it respond to near-infrared light and sunlight, which achieved the non-contact multistage shape memory performance, revealing the significant potential of the TPVs in the field of actuators., (Copyright © 2024. Published by Elsevier B.V.)

To test the effectiveness of phytoremediation of per- and polyfluoroalkyl substances (PFAS), we cultivated a mixture of nine grass-legume species native to Maine, US, in two types of soil amended with biosolids containing both pre-existing and spiked PFAS. To investigate how biochar amendment affects plant uptake of PFAS, two types of biochar at varying doses (i.e., 0%, 0.05%, 0.2%, 1%) were added to the biosolids before mixing with soil and cultivating plants. Our findings indicate that six representative PFAS, including short- and long-chain perfluoroalkyl carboxylic acids (PFCAs) (C 6 -C 8 ) and perfluoroalkylsulfonic acids (PFSAs) (C 4 , C 6 , and C 8 ), were effectively transferred from the biosolids amended soil (BAS) system to the harvestable grass-legume shoots. During the initial growth stage (Day 33), PFOA, PFHxS and PFOS were primarily detected in the grass shoots, showing a removal efficiency of 2-3%. As the growth period extended to Day 92, the uptake of short-chain PFBS, PFHxA, and PFHpA became increasingly dominant with an average removal efficiency of 5-20%. Notably, more than 10% of PFOA was uptaken by the shoots harvested from the Scantic soil amended with Biochar 2. These results were observed in grass-legume shoots when the soil was amended with a low dose of biochar (0.05%). However, when 1% biochar was added, PFAS were effectively stabilized, preventing their transfer to the above-ground plant compartments. Aside from different effect of different dose of the biochar, this study also revealed that plant's uptake of PFAS is highly dependent on soil properties. Overall, this study demonstrated the feasibility of using a grass-legume mix for removing PFAS from contaminated soil and raised the need of developing site-specific treatment strategies to maximize the performance of phytoremediation., Competing Interests: Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Studies on the prognosis of hepatitis B virus (HBV) reactivation following modern therapies for newly diagnosed MM (NDMM) are lacking. In this retrospective study, we aimed to assess the incidence, risk factors and prognosis of HBV reactivation in NDMM. A total of 33 of 355 patients with NDMM and HBV reactivation were included in this study. Multivariable analysis showed that hepatitis B surface antigen-positivity, hepatitis B core antibody-positivity, bortezomib-containing regimens, autologous stem cell transplantation, and gain of 1q21 were identified as independent risk factors of HBV reactivation in NDMM patients. The NDMM patients with HBV reactivation had poorer 3-year overall survival (OS) and progression-free survival (PFS) than did those without HBV reactivation, as confirmed by multivariate analysis. In conclusion, HBV reactivation in patients with NDMM constitutes a significant complication, correlating with reduced OS and PFS, and emerges as a potential adverse prognostic factor in the contemporary era of treatment., Competing Interests: The authors declare that they have no competing interests., (© 2024 Lv et al.)

Background: Pea protein isolate (PPI) is gaining increasing popularity in the food industry. It provides a diverse range of health benefits, such as hypoallergenic and gluten-free characteristics. However, the functional performance of PPI is hindered by its low solubility and poor stability. Therefore, in this article, PPI and dextran (DX) of different molecular weights were grafted to investigate the effects of grafting DX with different molecular weights on the interface properties and antioxidant properties of PPI. Additionally, the stability and digestive properties of the glycated PPI nanoemulsion system were explored., Results: The result showed that the grafting degree of PPI-DX conjugates (PPI-DC) decreased with an increase in the molecular weight of DX. Surface hydrophobicity, antioxidant activity and solubility of PPI-DC were significantly improved after grafting compared with PPI and PPI-DX mixtures (PPI-DM). Astaxanthin-loaded emulsions stabilized by grafted conjugates had smaller droplets and higher astaxanthin encapsulation rate compared to PPI emulsions. In vitro digestion demonstrated that the bioavailability of PPI-DC emulsions was higher than of PPI emulsion. Furthermore, after 24 days of storage, retention rate of astaxanthin-loaded emulsions prepared by conjugates remained above 70%, surpassing that of PPI emulsion., Conclusion: These results indicated that DX grafting can improve the emulsion properties of PPI. In addition, the DX with a molecular weight of 5 kDa showed the most significant improvement. This study contributes to the advancement of natural emulsifiers by modifying PPI through glycation, and furnishes a valuable reference for its utilization in functional foods. © 2024 Society of Chemical Industry., (© 2024 Society of Chemical Industry.)

Over 200 genetic mutations in copper-zinc superoxide dismutase (SOD1) have been linked to amyotrophic lateral sclerosis (ALS). Among these, two ALS-causing mutants, histidine-46→arginine (H46R) and glycine-85→arginine (G85R), exhibit a decreased capacity to bind metal ions. Here, we report two cryo-electron microscopy structures of amyloid fibrils formed by H46R and G85R. These mutations lead to the formation of amyloid fibrils with unique structures distinct from those of the native fibril. The core of these fibrils features a serpentine arrangement with seven or eight β strands, secured by a hydrophobic cavity and a salt bridge between arginine-85 and aspartic acid-101 in the G85R fibril. We demonstrate that these mutant fibrils are notably more toxic and capable of promoting the aggregation of wild-type SOD1 more effectively, causing mitochondrial impairment and activating ferroptosis in cell cultures, compared to wild-type SOD1 fibrils. Our study provides insights into the structural mechanisms by which SOD1 mutants aggregate and induce cytotoxicity in ALS.

Until now, palladium-catalyzed dearomative Heck reactions of indoles were largely limited to β-H elimination and nucleophilic capture of the transient alkyl-Pd(II) species. Herein, we disclose a novel palladium-catalyzed dearomative Heck/C(sp 2 )-H activation/decarboxylative cyclization of C2-tethered indoles. In this protocol, the alkyl-Pd(II) species formed by dearomatization of C2-tethered indoles is not terminated by common β-H elimination or nucleophilic capture, but rather generates C , C -palladacycle via C-H activation. The latter is intercepted by α-bromoacrylic acids to produce pentacyclic and heptacyclic fused indolines.

This study evaluated the safety, tolerability, pharmacodynamics, and pharmacokinetics of recombinant neorudin (EPR-hirudin [EH]) in patients with acute coronary syndrome (ACS), providing a basis for further therapeutic research. This open-label, single-center, nonrandomized, nonblinded, and noncontrolled trial categorized 24 patients with nonprogressive ACS who met the screening criteria into 3 groups. They received an intravenous injection of neorudin (0.4 mg/kg), followed by an intravenous drip at doses of 0.15, 0.30, and 0.45 mg/kg/h for 3 days in the low-, medium-, and high-dose groups, respectively. The safety, tolerability, pharmacodynamics, and pharmacokinetics of EH were assessed after treatment, indicating that neorudin was safe and well tolerated in nonprogressive ACS. No serious adverse events or clinical composite end points were observed. The activated partial thromboplastin time and thrombin time increased significantly and dose dependently following EH administration across all groups compared to pretreatment values. Conversely, thrombin activity significantly decreased after drug administration but returned to baseline levels shortly after drug withdrawal. Within the administered dose range, neorudin exposure increased with the dose, and its half-life was approximately 2 hours. Neorudin was found to be safe and tolerable for treating patients with nonprogressive ACS, demonstrating therapeutic efficacy at doses up to 0.45 mg/kg/h over a 3-day period., (© 2024 The Author(s). Clinical Pharmacology in Drug Development published by Wiley Periodicals LLC on behalf of American College of Clinical Pharmacology.)

In recent decades, protein-based therapy has garnered valid attention for treating infectious diseases, genetic disorders, cancer, and other clinical requirements. However, preserving protein-based drugs against degradation and denaturation during processing, storage, and delivery poses a formidable challenge. Herein, we designed a novel fluoroamphiphiles polymer to deliver protein. Two different formulations of nanoparticles, cross-linked (CNP) and micelle (MNP) polymer, were prepared rationally by disulfide cross-linked and thin-film hydration techniques, respectively. The size, zeta potential, and morphology of both formulations were characterized and the delivery efficacy of both in vitro and in vivo was also assessed. The in vitro findings demonstrated that both formulations effectively facilitated protein delivery into various cell lines. Moreover, in vivo experiments revealed that intramuscular administration of the two formulations loaded with a SARS-CoV-2 recombinant receptor-binding domain (RBD) vaccine induced robust antibody responses in mice without adding another adjuvant. These results highlight the potential use of our carrier system as a safe and effective platform for the in vivo delivery of subunit vaccines., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Mycelium polysaccharide (MPP) from Monascus pilosus with the compositions of glucose, galactose, mannose, glucosamine hydrochloride, rhamnose and arabinose, was obtained using alkaline extracting, and subsequently three purified components (MPP-0, MPP-0.1 and MPP-0.3) were separated. The purity and extraction volume of the MPP-0.1 fraction surpassed those of the other two groups, thus warranting its selection for subsequent experimental investigations. The sample MPP-0.1, with an average molecular weight of 3.7776 × 10 4  Da, exhibited exceptional thermal stability up to 170 °C. The main glycosidic linkage pattern of MPP-0.1 was structured as→[4)-α-D-Glcp-(1] 6  → 4)-α-D-Glcp-(1 → [2)-α-D-Manp-(1] 5  → 2)-α-D-Manp-(1 → 5)-β-D-Galf-(1 → 3)-β-D-Galf (1 → 3)-β-D-Galf-(1 → 3)-β-D-Galf-(1→, and branched Glcp, Manp, Galf fragments were connected with the main chain through →4, 6)-α-D-Glcp-(1→, →2, 6)-α-D-Manp-(1 → and →3, 6)-β-D-Galf-(1→. Besides, the up-regulated levels of Nitric oxide (NO), Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-1β (IL-1β) and other pro-inflammatory cytokines along with increased phagocytic activity revealed that MPP-0.1 has significant immunomodulatory effect, and can significantly enhance the proliferation and activation of RAW264.7 cells. Finally, the gene UGE (UDP-glucose 4-epimerase) was overexpressed in M. pilosus to increase the MPP production. Results showed that the biomass of the recombinant strain exhibited a remarkable increase of approximately 62.56 ± 1.50 % compared to that of the parental strain, and the extraction yield of MPP increased significantly by 83.19 ± 4.56 %., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Despite radiotherapy (RT) is recognized as preferred initial therapy for early-stage low-grade follicular lymphoma (FL) by many international practice guidelines, the medical oncologist has improperly underutilized RT, and diverse management strategies, including systemic therapy (ST), combined modality (CM) and watch and wait (WW), are still used. Except survival outcomes, previous studies concerned little about the treatment-related toxicity, which is also important factor in choosing initial management strategy, especially second primary malignancies (SPMs). The aim of this study was to compare the overall survival (OS) and the SPMs risk between different management strategies, which can provide guidance for the choice of optimal initial management strategy. Data was acquired from the Surveillance, Epidemiology, and End Results (SEER) database. Finally, A total 10,900 patients were identified, in which 930 cases developed SPMs. The use of radiotherapy (RT) has remained consistently low, with a utilization rate of around 20%, while most patients have received watchful waiting (WW) and systemic therapy (ST). In the rituximab era, multivariate analysis indicated that RT exhibited significantly superior OS and did not increase SPMs risk in comparison with ST and WW. At the same time, although there were no significant differences in OS between CM and RT, RT had significantly lower SPMs risk in comparison with CM. The use of RT improved the OS and did not increase the SPMs risk in comparison with other management strategies. Considering the low application rate of RT, oncologists should emphasize and increase the use of RT as an initial management strategy in patients with early-stage low-grade FL., (© 2024. The Author(s).)

Background: The optimal histologically complete (R0) resection methods of endoscopy for rectal neuroendocrine tumor (NET) ≤ 10 mm remains controversial. We aimed to assess the optimal endoscopic treatments for NETs., Methods: The retrospective enrolled patients (n = 208) with rectal NETs were divided into 3 subsets according to pathological tumor size: 2 - 3 mm, 4 - 5 mm, and 6 - 10 mm NETs. Factors associated with R0 resection according to different endoscopic treatments (accidental diagnostic biopsy by cold forceps, endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD)) and tumor size were investigated. All patients underwent follow-up and no local recurrence or metastasis were identified., Results: A total of 208 patients were enrolled. In patients with 2 - 3 mm NETs, the R0 resection rate was 100.0 % for biopsy, EMR, and ESD. The R0 resection rate for biopsy of 4 - 5 mm and 6 - 10 mm NETs was 34.3 % and 0.0 % respectively, which was inferior to the EMR/ESD rate (4 - 5mm: p < 0.001; 6 - 10 mm: p < 0.001: respectively). For patients with ≤ 10 mm NETs, EMR and ESD had a comparable en bloc (p = 0.082) and R0 resection rates (p = 0.651)., Conclusion: Accidental diagnostic biopsy by cold forceps could be considered as the possible treatment for 2 - 3 mm rectal NETs. And for patients with ≤ 10 mm rectal NETs, both EMR and ESD might be sufficient., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Background: Previous studies have revealed the importance of CYFRA 21-1 in the diagnosis of oral squamous cell cancer (OSCC). However, the results are inconsistent. This meta-analysis is to evaluate CYFRA 21-1's efficacy in distinguishing OSCC., Methods: Systematic searches of Web of Science, PubMed, and CNKI (1996-2024) were conducted following the Preferred Reporting ltems for Systematic Reviews and Meta-Analyses (PRISMA) guidelines., Results: Analysis of 693 patients and 548 controls yielded combined sensitivity (SEN) of 0.71 (95% CI: 0.68, 0.75), specificity (SPE) of 0.88 (95% CI: 0.85, 0.90), and area under the curve (AUC) of 0.927. Subgroup analysis showed higher SEN (0.88), SPE (0.93), and AUC (0.962) in saliva versus serum. Enzyme-linked immunosorbent assay (ELISA) demonstrated superior performance over electrochemiluminescent immunoassay (ECLIA) (AUC: 0.968 vs. 0.868)., Conclusion: CYFRA 21-1 is effective in OSCC diagnosis, with ELISA showing better sensitivity. Saliva emerges as a promising diagnostic medium compared to serum., Registration: PROSPERO CRD42024566835.

To reveal the effect of wheat flour particle size on the quality deterioration of quick-frozen dumpling wrappers (QFDW) during freeze-thawed (F/T) cycles, the components and physicochemical properties of wheat flours with five different particle sizes were determined and compared, along with the changes in texture and sensory properties, water status, and microstructure of QFDW during F/T cycles. Results showed that as particle size decreased, the damaged starch content and B-type starch content increased, the water absorption increased, and the gluten strength decreased. Furthermore, F/T cycles negatively impacted the quality of QFDW, evidenced by decreased texture properties and sensory evaluation score, water redistribution, higher freezable water content, and disruption of gluten network. Notably, QFDW made from larger particle size wheat flours required the shortest duration when traversing the maximum ice crystal formation zone. The QFDW made from larger particle size wheat flours formed a more stable starch-gluten matrix, which resisted the damage caused by ice recrystallization, demonstrating better water binding capacity and F/T resistance. The results may provide theoretical guidance for the study of QFDW quality and the moderate processing of wheat flour in actual production., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Trehalose-6-phosphate synthase (TPS) genes play an active role in the trehalose metabolism pathway that regulates the responses of plants to diverse stresses. However, the functional identification, comparison, and conservatism of TPS genes in the responses of woody plants, especially poplars, to drought stress remain unclear. Here, the trehalose content of 84K (Populus alba × P. glandulosa) poplars was down-regulated and PagTPS and PagTPP genes had diverse response patterns under drought stress. Physicochemical properties, expression patterns, and functions of PagTPS1 and PagTPS10, two class I members of TPS gene family, were identified and compared. Transgenic 84K poplars overexpressing PagTPS1 and PagTPS10 had significantly higher trehalose content with approximately 138% and 123%, respectively, and stronger drought tolerance compared to WT. PagTPS1 and PagTPS10 promoted the expression of TPPA genes and drought-responsive genes. Accordingly, poplars inhibiting PagTPS1 and PagTPS10 expression via RNA interference had lower trehalose content and drought tolerance. Simultaneously, overexpressing PagTPS1 and PagTPS10 improved the trehalose content and drought tolerance of Arabidopsis. Overall, we proposed a model of the effects of PagTPS1 and PagTPS10 as conservative regulators on the responses of plants to drought, which would provide new insights into the functional explorations of TPS genes in plants., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

C. aromaticum is widely cultivated for its aromatic, medicinal, and tea-applicable properties, earning the nickname 'lavender in composite'. Terpenoids are the major compounds of C. aromaticum fragrance. To reveal the molecular mechanisms of terpenoid biosynthesis in C. aromaticum, NGS and SMRT sequencing were employed to identify the key terpene synthase genes. A total of 59,903 non-redundant transcripts were obtained by the transcriptome analysis. Twenty-nine terpene synthase genes (TPSs) were identified, and phylogenetic analysis showed that they belong to four subfamilies of terpene synthases. Five CaTPSs were successfully cloned. Subcellular localization showed they were present in the nucleus and cytosol. Structure models of five terpene synthases were predicted, and molecular docking results showed good binding affinities with FPP/GPP. In vitro enzymatic tests showed that CaTPS7, CaTPS8, CaTPS10 and CaTPS20 could catalyze substrates to produce terpenoids. CaTPS7 and CaTPS20 were both able to effectively convert the precursor FPP into caryophyllene. CaTPS8 could convert FPP to trans-nerolidol and nerolidyl acetate, while CaTPS10 could convert FPP to elemene and aristolochene. This study lays the groundwork for further research to depict the metabolism network of terpenoid in C. aromaticum. These identical terpene synthase genes could be introduced into the cultivated chrysanthemums to enhance their fragrance., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Increasing evidence demonstrates that brain-derived neurotrophic factor (BDNF) can be regarded as a biomarker for major depression. Our previous work found that the ratio of mature BDNF (mBDNF) to precursor-BDNF (proBDNF) was a pivotal factor in the pathogenesis of major depressive disorder (MDD). But the mechanism behind the ratio is still obscure. Tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1) both play essential roles in depression by regulating the ratio of BDNF/proBDNF. In present study, we analyzed BDNF, proBDNF, tPA and PAI-1 in the peripheral blood in 57 MDD patients pre- and post-treatment and in 57 healthy controls. We verified that BDNF and tPA levels were significantly decreased, whereas proBDNF and PAI-1 levels elevated obviously in MDD group pre-treatment. And after 4 weeks SSRIs treatment, the BDNF and tPA levels increased while the proBDNF and PAI-1 levels reduced. The MDD pre-treatment group had the lowest ratio of BDNF to proBDNF compared to MDD post-treatment group and control group. Though the ratio of tPA/PAI-1 in MDD pre-treatment had not reached the significance, it was still the lowest one among the three groups. The combination of tPA + PAI + BDNF showed the best diagnostic value for MDD. In summary, our data suggested that the interaction between tPA and PAI-1 implicated to the MDD and the antidepressant treatment which might through regulating the BDNF/proBDNF ratio. The combination of tPA, PAI-1 and BDNF might offer a helpful way for MDD diagnosis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

Normal aging is accompanied by changes in brain structure and function associated with cognitive decline. Structural and functional abnormalities, particularly the prefrontal cortex (PFC) and subcortical regions, contributed to cognitive aging. However, it remains unclear how the synchronized changes in structure and function of individual brain regions affect the cognition in aging. Using 3D T1-weighted structural data and movie watching functional magnetic resonance imaging data in a sample of 422 healthy individuals (ages from 18 to 87 years), we constructed regional structure-function coupling (SFC) of cortical and subcortical regions by quantifying the distribution similarity of gray matter volume (GMV) and amplitude of low-frequency fluctuation (ALFF). Further, we investigated age-related changes in SFC and its relationship with cognition. With aging, increased SFC localized in PFC, thalamus and caudate nucleus, decreased SFC in temporal cortex, lateral occipital cortex and putamen. Moreover, the SFC in the PFC was associated with executive function and thalamus was associated with the fluid intelligence, and partially mediated age-related cognitive decline. Collectively, our results highlight that tighter structure-function synchron of the PFC and thalamus might contribute to age-related cognitive decline, and provide insight into the substrate of the thalamo-prefrontal pathway with cognitive aging., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 International Brain Research Organization (IBRO). Published by Elsevier Inc. All rights reserved.)

Background: Early prediction of hematoma expansion (HE) is important for the development of therapeutic strategies for spontaneous intracerebral hemorrhage (sICH). Radiomics can help to predict early hematoma expansion in intracerebral hemorrhage. However, complex image processing procedures, especially hematoma segmentation, are time-consuming and dependent on assessor experience. We provide a fully automated hematoma segmentation method, and construct a hybrid predictive model for risk stratification of hematoma expansion., Purpose: To propose an automatic approach for predicting early hemorrhage expansion after spontaneous intracerebral hemorrhage using deep-learning and radiomics methods., Methods: A total of 258 patients with sICH were retrospectively enrolled for model construction and internal validation, while another two cohorts (n=87, 149) were employed for independent validation. For hemorrhage segmentation, an iterative segmentation procedure was performed to delineate the area using an nnU-Net framework. Radiomics models of intra-hemorrhage and multiscale peri-hemorrhage were established and evaluated, and the best discriminative-scale peri-hemorrhage radiomics model was selected for further analysis. Combining clinical factors and intra- and peri-hemorrhage radiomics signatures, a hybrid nomogram was constructed for the early HE prediction using multivariate logistic regression. For model validation, the receiver operating characteristic (ROC) curve analyses and DeLong test were used to evaluate the performances of the constructed models, and the calibration curve and decision curve analysis were performed for clinical application., Results: Our iterative auto-segmentation model showed satisfactory results for hematoma segmentation in all four cohorts. The Dice similarity coefficient of this hematoma segmentation model reached 0.90, showing an expert-level accuracy in hematoma segmentation. The consumed time of the efficient delineation was significantly decreased, from 18 min to less than 2 min, with the assistance of the auto-segmentation model. The radiomics model of 2-mm peri-hemorrhage had a preferable area under ROC curve (AUC) of 0.840 (95 % confidence interval [CI]: 0.768, 0.912) compared with the original (0-mm dilatation) model with an AUC of 0.796 (95 % CI: 0.717, 0.875). The clinical-radiomics hybrid model showed better performances for HE prediction, with AUC of 0.853, 0.852, 0.772, and 0.818 in the training, internal validation, and independent validation cohorts 1 and 2, respectively., Conclusions: The fully automatic clinical-radiomics model based on deep learning and radiomics exhibits a good ability for hematoma segmentation and a favorable performance in stratifying HE risks., Competing Interests: Declaration of competing interest All authors have completed the ICMJE uniform disclosure form. The authors have no conflicts of interest to declare., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

This paper is devoted to the tracking control for an uncertain robotic system with both output constraint and dead-zone input. Remarkably, the distinctive characters of the system are reflected by system uncertainties and output constraint. First, more serious uncertainties are involved since unknown nonlinear dynamic matrices, external disturbance and the dead-zone input (see unknown slopes and break points therein) are simultaneously considered, but those of the related literature are not. Second, weaker conditions on the output constraint are allowed since the constraint functions considered are only first but not more order continuously differentiable while any their time derivatives are not necessarily available for feedback. This leads to the incapability of the traditional control schemes on this topic. To solve the control problem, a novel control framework is proposed based on time-varying feedback which overcomes the serious system uncertainties while relaxes the conditions on output constraints. Specifically, a state transformation with a time-varying gain is first introduced to derive a new system. Then, by using the traditional backstepping method with the introduction of the time-varying gain in the estimations of some uncertain terms, a time-varying feedback controller is explicitly designed, which ensures that all the states of the resulting closed-loop system are bounded while system output asymptotically tracks the reference signal without any violation of the output constraint. Finally, simulation results for two practical examples are provided to validate the effectiveness of the proposed theoretical results, and moreover, a comparison with PID method is given to show the superiority of the proposed method on tracking accuracy and robustness., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 International Society of Automation. Published by Elsevier Ltd. All rights reserved.)