1. The mechanism of Xihuang pills' intervention in the tumour immune microenvironment for the treatment of liver cancer based on the STAT3-PDL1 pathway.
- Author
-
Wang Y, Wang W, Liu K, Liu Y, Shen X, Li Q, Deng F, Hao X, and Wang Y
- Subjects
- Animals, Mice, Humans, Signal Transduction drug effects, Carcinoma, Hepatocellular drug therapy, Carcinoma, Hepatocellular immunology, Male, Cell Line, Tumor, Mice, Inbred BALB C, Mice, Inbred C57BL, Tumor Microenvironment drug effects, Drugs, Chinese Herbal pharmacology, Drugs, Chinese Herbal therapeutic use, STAT3 Transcription Factor metabolism, B7-H1 Antigen metabolism, Liver Neoplasms drug therapy, Liver Neoplasms immunology
- Abstract
Ethnopharmacological Relevance: Xihuang pills, a time-honored Chinese compound formula with a history spanning thousands of years, have demonstrated remarkable efficacy in treating various cancers, such as breast cancer, colon cancer, and liver cancer. Clinical applications over the years have established their effectiveness. Several scholars conducting experimental studies have elucidated the potent tumor-suppressing effects of Xihuang pills. While the inhibition of tumor vascular development and prevention of tumor cell invasion and metastasis have been well-explored mechanisms, the impact on the tumor immune microenvironment has received less attention. This study focuses on investigating the immune microenvironment adjustments induced by Xihuang pills in hepatocellular carcinoma., Aim of the Study: Tumour cells will find an escape phenomenon during tumour immunotherapy, which will affect immunotherapy results. We will research the regulation of the tumour immune microenvironment, to provide a more complete and precise basis for the elucidation of the mechanism of Xihuang pills in treating cancers. It provides new research ideas for people to treat liver cancer., Materials and Methods: Through in vivo and in vitro assessments confirming the intervention effects of Xihuang pills, we observed alterations in T cell typing, macrophage polarization, and tumor-associated cytokine levels. The primary active ingredients of Xihuang pills were identified using UPLC-MS/GC-MS, and relevant pathways in the treatment of hepatocellular carcinoma were predicted through network pharmacology. Combining the network pharmacology approach, we predicted the pathways relevant to Xihuang pills in treating hepatocellular carcinoma and experimentally validated the involvement of PD-1/PD-L1, a key immunity-related axis., Results: Xihuang Pill has a regulatory effect on the tumor immune microenvironment., Conclusions: The results indicated that Xihuang pills could impact splenic lymphocyte phenotyping, macrophage polarization, and IL-6 cytokine expression in liver cancer mice. The mechanism of action was associated with the regulation of the PD-1/PD-L1 signaling pathway by the STAT3 protein., Competing Interests: Declaration of competing interest All authors declare that they have no conflicts of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF