1. Ischemic cardiac stromal fibroblast-derived protein mediators in the infarcted myocardium and transcriptomic profiling at single cell resolution.
- Author
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Cha E, Hong SH, Rai T, La V, Madabhushi P, Teramoto D, Fung C, Cheng P, Chen Y, Keklikian A, Liu J, Fang W, and Thankam FG
- Subjects
- Humans, Stromal Cells metabolism, Interleukin-8 metabolism, Interleukin-8 genetics, Gene Expression Profiling, HSP90 Heat-Shock Proteins metabolism, HSP90 Heat-Shock Proteins genetics, HSP27 Heat-Shock Proteins metabolism, HSP27 Heat-Shock Proteins genetics, Cofilin 1 metabolism, Cofilin 1 genetics, Male, Myocardium metabolism, Myocardium pathology, Transcriptome, NF-E2-Related Factor 2 metabolism, NF-E2-Related Factor 2 genetics, Myocardial Infarction metabolism, Myocardial Infarction genetics, Myocardial Infarction pathology, Fibroblasts metabolism, Single-Cell Analysis
- Abstract
This article focuses on screening the major secreted proteins by the ischemia-challenged cardiac stromal fibroblasts (CF), the assessment of their expression status and functional role in the post-ischemic left ventricle (LV) and in the ischemia-challenged CF culture and to phenotype CF at single cell resolution based on the positivity of the identified mediators. The expression level of CRSP2, HSP27, IL-8, Cofilin-1, and HSP90 in the LV tissues following coronary artery bypass graft (CABG) and myocardial infarction (MI) and CF cells followed the screening profile derived from the MS/MS findings. The histology data unveiled ECM disorganization, inflammation and fibrosis reflecting the ischemic pathology. CRSP2, HSP27, and HSP90 were significantly upregulated in the LV-CABG tissues with a concomitant reduction ion LV-MI whereas Cofilin-1, IL8, Nrf2, and Troponin I were downregulated in LV-CABG and increased in LV-MI. Similar trends were exhibited by ischemic CF. Single cell transcriptomics revealed multiple sub-phenotypes of CF based on their respective upregulation of CRSP2, HSP27, IL-8, Cofilin-1, HSP90, Troponin I and Nrf2 unveiling pathological and pro-healing phenotypes. Further investigations regarding the underlying signaling mechanisms and validation of sub-populations would offer novel translational avenues for the management of cardiac diseases., (© 2024. The Author(s).)
- Published
- 2024
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