1. Label-free detection of endocrine disrupting chemicals by integrating a competitive binding assay with a piezoelectric ceramic resonator.
- Author
-
Hu LS, Fong CC, Zou L, Wong WL, Wong KY, Wu RS, and Yang M
- Subjects
- Ceramics chemistry, Endocrine Disruptors isolation & purification, Endocrine Disruptors toxicity, Environmental Monitoring, Estradiol toxicity, Estrogen Receptor alpha drug effects, Estrogen Receptor alpha metabolism, Estrone toxicity, Gold chemistry, Humans, Metal Nanoparticles chemistry, Seawater analysis, Water Pollutants, Chemical toxicity, Benzhydryl Compounds isolation & purification, Biosensing Techniques methods, Estradiol isolation & purification, Estrone isolation & purification, Phenols isolation & purification
- Abstract
A piezoelectric biosensor for detection of endocrine disrupting chemicals (EDCs) was developed by incorporating chemical/biochemical recognition elements on the ceramic resonator surface for competitive binding assays. A facile electrodeposition was employed to modify the sensor surface with Au nanoparticles, which increased the surface area and enhanced the binding capacity of the immobilized probes. Thiol-labeled long chain hydrocarbon with bisphenol A (BPA) as head group was synthesized and self-assembled on the Au nanoparticle surface as the sensing probes, which showed a linear response upon the binding of estrogen receptor (ER-α) ranging from 1 to 30 nM. Detection of estrone, 17β-estradiol and BPA was achieved by integrating a competitive binding assay with the piezoelectric sensor. In this detection scheme, different concentrations of EDCs were incubated with 30 nM of ER-α, and the un-bounded ER-α in the solution was captured by the probes immobilized on the ceramic resonator, which resulted in the frequency changes for different EDCs. The biosensor assay exhibited a linear response to EDCs with a low detection limit of 2.4-2.9 nM (S/N=3), and required only a small volume of sample (1.5 µl) with the assay time of 2h. The performance of the biosensor assay was also evaluated for rapid and facile determination of EDCs of environmental relevant concentrations in drinking water and seawater, and the recovery rate was in the range between 94.7% and 109.8%., (© 2013 Elsevier B.V. All rights reserved.)
- Published
- 2014
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