1. Studies on HOXB4 expression during differentiation of human cytomegalovirus-infected hematopoietic stem cells into lymphocyte and erythrocyte progenitor cells.
- Author
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Wen-jun L, qu-lian G, Hong-ying C, Yan Z, and Mei-Xian H
- Subjects
- Cell Proliferation, Cytomegalovirus, Erythrocytes metabolism, Fetal Blood cytology, Fetal Blood metabolism, Gene Expression Regulation, Hematopoietic Stem Cells metabolism, Hematopoietic Stem Cells virology, Homeodomain Proteins genetics, Humans, Lymphocytes metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Transcription Factors genetics, Transcription, Genetic, Tretinoin pharmacology, Tretinoin physiology, Cell Differentiation, Cytomegalovirus Infections metabolism, Erythrocytes cytology, Hematopoietic Stem Cells physiology, Homeodomain Proteins metabolism, Lymphocytes cytology, Transcription Factors metabolism
- Abstract
We investigated the role of homeobox B4 (HOXB4) mRNA/protein expression induced by human cytomegalovirus (HCMV) and/or all-trans retinoic acid (ATRA) in proliferation and committed differentiation of human cord blood hematopoietic stem cells (HSCs) into colony-forming-units of T-lymphocyte (CFU-TL) and erythroid (CFU-E) progenitors in vitro. Twelve cord blood samples were collected from the fetal placenta umbilical vein and cultured in vitro. The proliferation and differentiation of cord blood HSCs into CFU-TL and CFU-E were continuously disrupted with HCMV-AD169 and/or 6 × 10(-8) mol/l of ATRA. HOXB4 mRNA/protein expression in CFU-TL and CFU-E was detected in control, ATRA, HCMV and ATRA + HCMV groups on days 3, 7, and 12 of culture by fluorescent qRT-PCR/western blot. We found that HOXB4 mRNA/protein expression was detectable on day 3, increased on day 7 and was highest on day 12. HOXB4 mRNA/protein expression in HCMV group was downregulated compared with control group (P < 0.05). However, the levels were significantly upregulated in HCMV + ATRA group compared with HCMV group (P < 0.05). We concluded that the abnormal HOXB4 mRNA/protein expression induced by HCMV could play a role in hematopoietic damage. ATRA, at the concentration used, significantly up-regulated HOXB4 mRNA/protein expression in normal lymphocyte and erythrocyte progenitor cells as well as in HCMV-infected cells.
- Published
- 2012
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