1. Extracellular vesicles loaded with ApoB-100 protein affect the occurrence of coronary heart disease in patients after injury of spinal cord.
- Author
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Wu C, Chen J, Zhang J, Hong H, Jiang J, Ji C, Li C, Xia M, Xu G, and Cui Z
- Subjects
- Animals, Humans, Rats, Male, Neurons metabolism, Female, Macrophages metabolism, Middle Aged, Disease Models, Animal, Apoptosis, Rats, Sprague-Dawley, Apolipoprotein B-100 metabolism, Extracellular Vesicles metabolism, Coronary Disease metabolism, Coronary Disease pathology, Spinal Cord Injuries metabolism, Spinal Cord Injuries pathology, Adaptor Proteins, Vesicular Transport metabolism, Adaptor Proteins, Vesicular Transport genetics
- Abstract
Spinal cord injury (SCI) patients have an increased susceptibility to coronary heart disease (CHD) due to dysregulated lipid deposition. We conducted a comprehensive investigation to gain insights into the specific roles of Apolipoprotein B-100 (APOB-100) in the development of CHD in patients suffering from SCI. First, we established an SCI rat model through semitransection. APOB-100 expression in plasma exosomes obtained from patients were determined. Subsequently, we found APOB-100 affected macrophage polarization when treating co-cultured neurons/macrophages lacking Sortilin with extracellular vesicles derived from SCI rats, where APOB-100 co-immunoprecipitated with Sortilin. Moreover, APOB-100 upregulation reduced neuronal cell viability and triggered apoptosis by upregulating Sortilin, leading to a decline in the Basso, Beattie, and Bresnahan (BBB) scale, exacerbation of neuron injury, increased macrophage infiltration, and elevated blood lipid-related indicators in SCI rats, which could be reversed by silencing Sortilin. In conclusion, APOB-100 from post-SCI patients' extracellular vesicles upregulates Sortilin, thereby endangering those patients to CHD., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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