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Afforestation on degraded croplands has been proposed as an effective measure to promote ecosystem functions including soil organic carbon (SOC) sequestration. Glomalin-related soil protein (GRSP) plays a crucial role in promoting the accumulation and stability of SOC. Nevertheless, mechanisms underlying the effects of afforestation on GRSP accumulation have not been well elucidated. In the present study, 14 pairs of maize fields and plantation forests were selected using a paired-site approach in a karst region of southwest China. By measuring soil GRSP and a variety of soil biotic and abiotic variables, the pattern of and controls on GRSP accumulation in response to afforestation were explored. The average content of total GRSP (T-GRSP) and its contribution to SOC in the maize field were 5.22 ± 0.29 mg g -1 and 42.33 ± 2.25%, and those in the plantation forest were 6.59 ± 0.32 mg g -1 and 25.77 ± 1.17%, respectively. T-GRSP content was increased by 26.4% on average, but its contribution to SOC was decreased by 39.1% following afforestation. T-GRSP content decreased as soil depth increased regardless of afforestation or not. Afforestation increased T-GRSP indirectly via its positive effects on arbuscular mycorrhizal fungi biomass, which was stimulated by afforestation through elevating fine root biomass or increasing the availability of labile C and N. The suppressed contribution of T-GRSP to SOC following afforestation was due to the relatively higher increase in other SOC components than T-GRSP and the significant increase of soil C:N ratio. Our study reveals the mechanisms underlying the effects of afforestation on T-GRSP accumulation, and is conducive to improving the mechanistic understanding of microbial control on SOC sequestration following afforestation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Soil depth plays a crucial role in shaping the interactions between soil microbes and nutrient availability. However, there is limited understanding of how bacterial, fungal, and protistan communities respond to different soil depths, particularly in the unique geological context and soil properties of karst regions. Organic matter, total nitrogen, and phosphorus, ammonium, nitrate, and plant root biomass, as well as bacterial and fungal abundances, bacterial and protistan diversity were higher in the 0-20 cm soil layer than those in the 20-40 cm and soil-rock mixing layers. In contrast, soil pH was higher in the 20-40 cm and soil-rock mixing layers than that in the 0-20 cm soil layer. The soil exchange of calcium, nitrate, and root biomass were identified as the primary factors regulating microbial assemblages across the depth transect. Moreover, co-occurrence network analysis revealed a greater degree of connectivity between protistan taxa and fungal taxa in the 0-20 cm soil layer than those in the 20-40 cm and soil-rock mixing layers. In contrast, the number of association links between protist-bacteria and bacteria-bacteria was higher in the soil-rock mixing layers compared to the 0-20 cm soil layer. Actinobacteria, Ascomycota, and unclassified protistan taxa were identified as keystones, displaying the highest number of connections with other microbial taxa. Collectively, these results suggested that the increased plant root biomass, coupled with sufficient available nutrient inputs in the upper 0-20 cm soil layer, facilitates strong interactions among fungal and protistan taxa, which play crucial roles in the topsoil. However, as nutrients become less available with increasing depth, competition among bacterial taxa and the predation between bacterial and protistan taxa intensify. Therefore, these findings indicate the interactions among keystone taxa at different soil depths has the potential to generate ecological implications during vegetation restoration in fragile ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. We have read and understood your journal's policies, and we believe that neither the manuscript nor the study violates any of these., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The influence of lithology and slope position on arbuscular mycorrhizal fungi (AMF) communities has been explored in various ecosystems, but there is a limited understanding of these mechanisms in karst regions. This study focused on typical karst hills with contrasting lithologies, specifically dolomite and limestone. Additionally, three slope positions (upper, middle, and lower) were investigated within each hill in karst forest ecosystems. Total phosphorus (TP) content in the soil was higher in dolomite compared to limestone. Conversely, exchangeable calcium (Ca) was lower in dolomite than in limestone. Notably, the lithology, rather than the slope position, exerted a significant impact on AMF diversity and abundance and the presence of specific AMF taxa. Dolomite exhibited greater AMF richness and a higher Shannon index in comparison to limestone when not accounting for slope position. The AMF community composition differed between dolomite and limestone. For instance, without considering slope position, the relative abundance of Acaulospora , Diversispora , and Paraglomus was higher in dolomite than in limestone, while the relative abundance of Claroideoglomus displayed an opposing trend. Furthermore, a more complex interaction among AMF taxa was observed in dolomite as compared to limestone, as evidenced by an increase in the number of nodes and edges in the co-occurrence networks within the dolomite. The genera Glomus , Claroideoglomus , and Diversispora exhibited a higher number of links with each other and with other AMF taxa. The study identified TP and Ca as the primary factors determining variations in AMF diversity between dolomite and limestone. Consequently, it is imperative to consider the underlying lithology and soil conditions when addressing the restoration of degraded karst hilly areas.

Considering the crucial role of soil diazotrophs and root arbuscular mycorrhizal fungi (AMF) in soil nutrient cycling during ecosystem restoration, diazotroph and AMF communities may be determined by slope position. However, the effect of slope position on diazotroph and AMF abundance, diversity, and community composition of karst ecosystems remains unknown. In this study, soil diazotrophs and root AMF characteristics on varying slope positions were assessed in a karst shrub ecosystem. The results displayed that the abundance of soil diazotrophs and root AMF diversity were significantly affected by slope position. Diazotroph abundance accompanied by soil nutrient and plant richness was higher on the lower slopes than the upper slopes, whereas root AMF diversity displayed the opposite trend. The soil diazotroph and root AMF community composition differed among the upper, middle, and lower slopes. The dominant taxa of soil diazotrophs and root AMF at the order level were Rhizobiales and Glomerales, respectively. Moreover, the diazotroph order of Nostocales and the AMF order of Paraglomerales were richer on the upper slopes than on the lower slopes. The slope position directly affected the plant diversity and soil nutrient distribution, indirectly affecting the diazotroph and AMF communities. Increased available nitrogen on the lower slope caused great diazotroph abundance by stimulating plant growth with sufficient carbohydrates. However, low soil nutrients and plant diversity but high plant root biomass induced more root AMF diversity on the upper slope than on the lower slope. Therefore, this study expands the knowledge of soil diazotroph and root AMF ecological functions along different slope positions during vegetation recovery for the successive stages of grass and shrub in the karst region.

In order to investigate the carbon sequestration potential of biochar on soil, citrus orchard soils with a forest age of 5 years was taken as the research object, citrus peel biochar (OBC) and magnesium-modified citrus peel biochar (OBC-mg) were selected as additive materials, and organic carbon mineralization experiments were carried out in citrus orchard soil. OBC and OBC-Mg were applied to citrus orchard soils at four application rates (0, 1, 2, and 4%), and incubated at a constant temperature for 100 days. Compared with CK, the cumulative mineralization of soil organic carbon decreased by 5.11% with 1% OBC and 2.14% with 1% OBC-Mg. The application of OBC and OBC-Mg significantly increased the content of soil organic carbon fraction, while the content of soil organic carbon fraction was higher in OBC-Mg treated soil than in OBC treated soil. Meanwhile, the cumulative mineralization of soil organic carbon was significantly and positively correlated with the activities of soil catalase, urease and sucrase. The enzyme activities increased with the cumulative mineralization of organic carbon, and the enzyme activities of the OBC-Mg treated soil were significantly higher than those of the OBC treated soil. The results indicated that the OBC-Mg treatment inhibited the organic carbon mineralization in citrus orchard soils and was more favorable to the increase of soil organic carbon fraction. The Mg-modified approach improved the carbon sequestration potential of biochar for citrus orchard soils and provided favorable support for the theory of soil carbon sink in orchards., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Hu, Huang, Zhou, Qin, He, Deng and Li.)

Microbial communities contribute to sustaining the function of terrestrial ecosystems and are influenced by soil type and climate gradients. The effects of karst and non-karst soils on bacterial and fungal profiles for seven climate gradients were assessed to better understand bacterial and fungal diversity and community composition in response to soil type with changes in soil physicochemical properties under different temperatures and precipitations. Bacterial and fungal abundance, diversity, and community composition differed between karst and non-karst forests. Bacterial and fungal richness, Shannon index, and bacterial abundance in karst forests were higher than non-karst forests, but the fungal abundance was lower. Mean annual temperature was negatively correlated with bacterial diversity in the karst forest and fungal abundance in karst and non-karst forests. The community composition of bacteria and fungi differed among these two soil types. The karst forest had greater connectivity among bacterial and fungal communities than non-karst forests. The bacterial members of Acidobacteria, Proteobacteria, Actinobacteria, and fungal groups of Ascomycota and Basidiomycota were mainly connected with other taxa in the network, implying that taxa were associated with highly functional potential. The relative abundance of Actinobacteria and Ascomycota was higher in karst than in non-karst forests. Proteobacteria and Basidiomycota showed the opposite results. A random forest and multiple regression tree analyses revealed that soil properties, specifically pH, calcium, and total nitrogen, were the main factors influencing the variation in bacterial and fungal profiles between karst and non-karst forests. This study provides novel evidence that the abundant microbial taxa were kinless hubs in co-occurrence patterns. Controlling complex networks of species interactions may contribute to improving soil nutrient processes rather than microbial diversity, enhancing our understanding of developing sustainable recovery strategies in fragile ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. We have read and understood your journal's policies, and we believe that neither the manuscript nor the study violates any of these., (Copyright © 2022 Elsevier B.V. All rights reserved.)

The decomposition and transformation of organic matters during composting process are performed by various microorganisms. However, the bacterial communities and their functions usually vary with composting materials. Here the dominant bacterial genera and their functions were identified at the thermophilic phase during composting of mulberry branches with silkworm excrement (MSE), pig manure (MPM) and cow manure (MCD). The activities of β-glucosidase and endoglucanase were highest for MCD (1.31 and 17.15 µg g -1 min -1 ) and lowest for MPM (0.92 and 14.22 µg g -1 min -1 ). Random Forest model and correlation analysis revealed that Stenotrophomonas, Bacillus, and Sinibacillus were the dominant bacterial genera involved in lignocellulose degradation regardless of composting materials. Carbohydrate metabolism, amino acid metabolism, and DNA replication and repair were primary functions of the bacterial communities for the three types of composting. The quantification of lignocellulose degradation genes further verified the dominant functions of the bacterial communities., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Vegetation restoration has been proposed as an effective strategy for increasing soil organic carbon (SOC) sequestration. However, the responses of SOC to managed and natural vegetation restoration strategies at a large scale are poorly understood due to the varying SOC components and changing climatic conditions. Here, we measured bulk SOC, particulate organic carbon (POC), and mineral-associated organic carbon (MOC) after 15 years of vegetation restoration along an elevation gradient with a corresponding temperature gradient in the calcareous soils of karst region, Southwest China. We compared managed plantation forest and naturally recovered shrubland vegetation restoration strategies, using cropland and mature forest as references. Overall, we found that the SOC and POC densities in both plantation forest and shrubland were significantly higher than in the cropland but lower than in the mature forest. There were no significant differences in the SOC pool between the plantation forest and shrubland. Furthermore, the relative changes in the SOC and POC densities increased with increasing mean annual temperature in the plantation forest and shrubland. Our results showed that both vegetation restoration strategies, characterized by higher soil microbial abundance and exchangeable Ca concentration, were beneficial to POC but not MOC accumulation, and sufficiently compensated SOC decomposition at lower elevation with higher MAT. Our results highlight the potential of both vegetation restoration strategies for promoting SOC accumulation in warmer karst regions and emphasize the necessity to understand soil carbon stabilization mechanisms in calcareous soils., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Soil diazotrophs have been known to be essential in biological nitrogen (N) fixation, which contributes to the sustainability of agricultural ecosystems. However, there remains an inadequacy of research on the effects of different N inputs from N fertilization and from symbiotic N fixation associated with legumes on the diazotroph communities in agricultural ecosystems. Hence, we investigated the variations in diazotroph abundance and community composition as well as the soil properties with different N inputs in the Guimu-1 hybrid elephant grass cultivation on karst soils in China. We conducted six different N treatments: control, Amorpha fruticosa planting at a spacing of 1.5 × 2 m (AFD1), A. fruticosa planting at a spacing of 1 × 2 m (AFD2), N fertilization (N), A. fruticosa planting at a spacing of 1.5 × 2 m with N fertilization (AFD1N), and A. fruticosa planting at a spacing of 1 × 2 m with N fertilization (AFD2N). Our results showed that the interaction between sampling time and N fertilization significantly affected the diazotroph abundance. In July, the diazotroph abundance significantly decreased in the N fertilization treatments: N, AFD1N, and AFD2N, compared to that in the control. The richness and Chao1 estimator of diazotrophs significantly increased in AFD2N and AFD1 correspondingly in December and July, relative to those in the control. Co-occurrence networks showed species-species interactions with high negative correlations that occurred more in the control than in the N input plots. The N input from N fertilization and legume planting directly increased the ammonium N and nitrate N and consequently affected the dissolved organic N and pH of the soil, thereby altering the diazotroph abundance and richness. Our findings demonstrated that both N fertilization and legumes could reduce the interspecific competition among diazotroph species by providing greater N availability in the forage grass., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Legumes can increase nitrogen (N) input to soil via N 2 fixation, and arbuscular mycorrhizal fungi (AMF) can colonize legumes, which further promotes the acquisition of nutrients such as phosphorus (P). Nevertheless, little is known about how different legume species or planting densities affect soil AMF communities. We measured soil AMF abundance, diversity, and community composition in two legume species that had been planted at two densities in a karst grassland. Five treatments were used: control (CK), Amorpha fruticosa at 1.5 × 2 m density (AFD1), A. fruticosa at 1 × 1 m density (AFD2), Indigofera atropurpurea at 1.5 × 2 m density (IAD1), and I. atropurpurea at 1 × 1 m density (IAD2). The results showed that A. fruticosa plots were significantly richer in Redeckera spp., while I. atropurpurea plots were richer in Septoglomus. AMF abundance in AFD1, AFD2, and IAD1 was significantly higher than in CK, but AMF abundance in IAD2 was significantly lower than that in the other treatments. AMF richness and Chao1 estimator in AFD1 were significantly higher than in CK. Funneliformis, Septoglomus, and Acaulospora were significantly more abundant in IAD2 than in the other treatments. The interaction between legume species and density had a significant effect on AMF abundance and community composition. AMF abundance and diversity were significantly negatively and positively correlated with available P and microbial biomass N, respectively. These results suggest that different species and densities of legumes may increase available N, which could improve AMF abundance and alleviate soil P deficiencies. Planting A. fruticosa or I. atropurpurea at a low density may be an effective method to increase AMF colonization of roots, and thus, nutrient transport in karst grasslands., (Copyright © 2019. Published by Elsevier B.V.)

The first funding No. should be 41671298 instead of 41301298.

In this study, we aimed to investigate lignin accumulation and its relationship with the composition of bacterial laccase-like genes in three arable lands (i.e., upland limestone soil (UL), upland red soil (UR), and upland-paddy rotation red soil (UPR)), which are subjected to long-term straw incorporation. After 9-13 years of straw incorporation, the lignin content significantly increased from 337.1, 414.5, and 201.6 mg/kg soil to 2096.5, 2092.4, and 1972.2 mg/kg soil in UL, UR, and UPR, respectively. The dominant lignin monomer changed from vanillyl (V)-type to cinnamyl (C)-type in UR. Both V- and C-types were the dominant monomers in UPR, and V-type monomer remained the dominant monomer in UL. Compared with the treatment without straw, straw incorporation significantly promoted the activity of laccase enzyme and the abundance of bacterial laccase-like genes in all soils. The redundancy analysis showed that the main influencing factors on lignin accumulation patterns with straw incorporation were the laccase enzyme activity, nitrogen availability, and some specific bacterial communities possessing the laccase-like genes (e.g., Thermotogae and Acidobacteria). The variation partitioning analysis confirmed that the strongest influencing factor on lignin accumulation was the composition of bacterial laccase-like genes (explained 31.4% of variance). The present study provides novel insights into the importance of bacterial laccase-like genes in shaping lignin monomer accumulation with straw incorporation in arable soils.

The addition of exogenous inorganic carbon (CaCO3) and organic carbon has an important influence on soil organic carbon (SOC) mineralization in karst soil, but the microbial mechanisms underlying the SOC priming effect are poorly understood. We conducted a 100-day incubation experiment involving four treatments of the calcareous soil in southwestern China's karst region: control, (14)C-labeled rice straw addition, (14)C-labeled CaCO3 addition, and a combination of (14)C-labeled rice straw and CaCO3. Changes in soil microbial communities were characterized using denaturing gradient gel electrophoresis with polymerase chain reaction (PCR-DGGE) and real-time quantitative PCR (q-PCR). Both (14)C-rice straw and Ca(14)CO3 addition stimulated SOC mineralization, suggesting that organic and inorganic C affected SOC stability. Addition of straw alone had no significant effect on bacterial diversity; however, when the straw was added in combination with calcium carbonate, it had an inhibitory effect on bacterial and fungal diversity. At the beginning of the experimental period, exogenous additives increased bacterial abundance, although at the end of the 100-day incubation bacterial community abundance had gradually declined. Incubation time, exogenous input, and their interaction significantly affected SOC mineralization (in terms of priming and the cumulative amount of mineralization), microbial biomass carbon (MBC), and microbial community abundance and diversity. Moreover, the key factors influencing SOC mineralization were MBC, bacterial diversity, and soil pH. Overall, these findings support the view that inorganic C is involved in soil C turnover with the participation of soil microbial communities, promoting soil C cycling in the karst region., (Copyright © 2016 Elsevier B.V. All rights reserved.)

Arbuscular mycorrhizal (AM) fungi and nitrogen-fixing bacteria play important roles in plant growth and recovery in degraded ecosystems. The desertification in karst regions has become more severe in recent decades. Evaluation of the fungal and bacterial diversity of such regions during vegetation restoration is required for effective protection and restoration in these regions. Therefore, we analyzed relationships among AM fungi and nitrogen-fixing bacteria abundances, plant species diversity, and soil properties in four typical ecosystems of vegetation restoration (tussock (TK), shrub (SB), secondary forest (SF), and primary forest (PF)) in a karst region of southwest China. Abundance of AM fungi and nitrogen-fixing bacteria, plant species diversity, and soil nutrient levels increased from the tussock to the primary forest. The AM fungus, nitrogen-fixing bacterium, and plant community composition differed significantly between vegetation types (p < 0.05). Plant richness and pH were linked to the community composition of fungi and nitrogen-fixing bacteria, respectively. Available phosphorus, total nitrogen, and soil organic carbon levels and plant richness were positively correlated with the abundance of AM fungi and nitrogen-fixing bacteria (p < 0.05). The results suggested that abundance of AM fungi and nitrogen-fixing bacteria increased from the tussock to the primary forest and highlight the essentiality of these communities for vegetation restoration.

Laccase enzymes produced by both soil bacteria and fungi play important roles in refractory organic matter turnover in terrestrial ecosystems. We investigated the abundance and diversity of fungal laccase genes and bacterial laccase-like genes in soil from subtropical arable lands, and identified which microbial group was associated with laccase activity. Compared with fungal laccase genes, the bacterial laccase-like genes had greater abundance, richness and Shannon-Wiener diversity. More importantly, laccase activity can be explained almost exclusively by the bacterial laccase-like genes, and their abundance had significant linear relationship with laccase activity. Thus, bacterial laccase-like gene has great potential to be used as a sensitive indicator of laccase enzyme for refractory organic matter turnover in subtropical arable lands.

Karst ecosystems are fragile and are in many regions degraded by anthropogenic activities. Current management of degraded karst areas focuses on aboveground vegetation succession or recovery and aims at establishing a forest ecosystem. Whether progressive succession of vegetation in karst areas is accompanied by establishment of soil biota is poorly understood. In the present study, soil microbial and nematode communities, as well as soil physico-chemical properties were studied along a progressive succession of secondary vegetation (from grassland to shrubland to forest) in a karst area in southwest China. Microbial biomass, nematode density, ratio of fungal to bacterial biomass, nematode structure index, and nematode enrichment index decreased with the secondary succession in the plant community. Overall, the results indicated a pattern of declines in soil biota abundance and food web complexity that was associated with a decrease in soil pH and a decrease in soil organic carbon content with the progressive secondary succession of the plant community. Our findings suggest that soil biota amendment is necessary during karst ecosystem restoration and establishment and management of grasslands may be feasible in karst areas.

Soil organic matter (SOM) content in paddy soils is higher than that in upland soils in tropical and subtropical China. The dissolved organic matter (DOM) concentration, however, is lower in paddy soils. We hypothesize that soil moisture strongly controls the fate of DOM, and thereby leads to differences between the two agricultural soils under contrasting management regimens. A 100-day incubation experiment was conducted to trace the fate and biodegradability of DOM in paddy and upland soils under three moisture levels: 45%, 75%, and 105% of the water holding capacity (WHC). (14)C labeled DOM, extracted from the (14)C labeled rice plant material, was incubated in paddy and upland soils, and the mineralization to (14)CO2 and incorporation into microbial biomass were analyzed. Labile and refractory components of the initial (14)C labeled DOM and their respective half-lives were calculated by a double exponential model. During incubation, the mineralization of the initial (14)C labeled DOM in the paddy soils was more affected by moisture than in the upland soils. The amount of (14)C incorporated into the microbial biomass (2.4-11.0% of the initial DOM-(14)C activity) was less affected by moisture in the paddy soils than in the upland soils. At any of the moisture levels, 1) the mineralization of DOM to (14)CO2 within 100 days was 1.2-2.1-fold higher in the paddy soils (41.9-60.0% of the initial DOM-(14)C activity) than in the upland soils (28.7-35.7%), 2) (14)C activity remaining in solution was significantly lower in the paddy soils than in the upland soils, and 3) (14)C activity remaining in the same agricultural soil solution was not significantly different among the three moisture levels after 20 days. Therefore, moisture strongly controls DOM fate, but moisture was not the key factor in determining the lower DOM in the paddy soils than in the upland soils. The UV absorbance of DOM at 280 nm indicates less aromaticity of DOM from the paddy soils than from the upland soils. At any of the moisture levels, much more labile DOM was found in paddy soils (34.3-49.2% of the initial (14)C labeled DOM) compared with that in upland soils (19.4-23.9%). This demonstrates that the lower DOM content in the paddy soil compared with that in the upland soil is probably determined by the less complex components and structure of the DOM., (Copyright © 2014 Elsevier B.V. All rights reserved.)

Soil ammonia oxidizers play a critical role in nitrogen cycling and ecological restoration. The composition and structure of soil ammonia oxidizers and their impacting factors were studied in four typical ecosystem soils, tussock (T), shrub (S), secondary forest (SF), and primary forest (PF), during vegetation restoration in the Karst region of Southwest China. The composition and structure of the ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) communities were characterized by sequencing the amoA and arch-amoA genes, respectively. The diversity of soil ammonia oxidizers (except in S) and plant Shannon diversity index gradually increased with vegetation restoration, and the ammonia oxidizer communities differed significantly (p < 0.001). Amplicons of AOA from the Nitrososphaera cluster dominated all four ecosystem soils. AOB Nitrosospira cluster 3b only appeared in PF and SF soils, while Nitrosospira cluster 3a species were found in all soils. Changes in AOB paralleled the changes in soil ammonium content that occurred with vegetation restoration. Redundancy analysis showed that the distribution of dominant AOB species was linked to pH, soil urease activity, and soil C/N ratio, whereas the distribution of dominant AOA species was mainly influenced by litter nitrogen content and C/N ratio. These results suggested that the composition and structure of the AOB community were more sensitive to changes in vegetation and soil ammonium content, and may be an important indicator of nitrogen availability in Karst ecosystem soils., (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Background: Soil carbon management at landscape scale requires reliable information on the spatial distribution of soil organic carbon (SOC). However, how to improve the accuracy of spatial prediction is not well addressed in the karst region of southwestern China. This study evaluates the performance of univariate kriging (ordinary kriging (OK)) and hybrid kriging (co-kriging (CK), regression kriging (RK) and residual maximum likelihood (REML)) in mapping the spatial distribution of SOC at a depth of 0-15 cm. Terrain attributes and the normalised difference vegetation index (NDVI) were used as ancillary variables., Results: The distribution of SOC was significantly related to NDVI and terrain attributes. Furthermore, geostatistical analyses reflected a moderately structured spatial correlation of SOC. Regression analyses identified the NDVI and slope as the best predictors for describing the spatial pattern of SOC. Combined with NDVI and slope gradient, REML and RK performed better in increasing map prediction accuracy and decreasing the soothing effect of kriging., Conclusion: The spatial pattern of SOC was controlled by topography and cultivation activity. The predictive abilities of OK and CK were limited. Combined with the auxiliary variables, REML and RK can improve the prediction accuracy. This study is beneficial for the further research of precise SOC management in the typical karst landscape., (Copyright © 2012 Society of Chemical Industry.)

Background: Organic substrates and calcium are important factors controlling organic matter turnover in Karst soils. To understand their effects on soil organic carbon (SOC) mineralization, an incubation experiment was conducted involving a control treatment (CK), the addition of a (14)C-labeled rice straw (T1), CaCO(3) (T2), and both (14)C-labeled rice straw and CaCO(3) (T3) to two types of Karst soils (terra fusca and rendzina) and a red soil from southwestern China., Results: Cumulative mineralization of the rice straw over 100 days in rendzina (22.96 mg kg(-1)) and terra fusca (23.19 mg kg(-1)) was higher than in the red soil (15.48 mg kg(-1); P < 0.05). Cumulative mineralization of native SOC decreased following addition of (14)C-labeled rice straw in the rendzina and terra fusca but increased in the red soil (negative and positive priming effects on native SOC). The turnover times of (14)C-labeled microbial biomass C (MBC) in the red soil, terra fusca and rendzina were 71 ± 2, 243 ± 20 and 254 ± 45 days, respectively. By adding CaCO(3), the accumulation of SOC was greater in the Karst soils than in the red soil., Conclusion: Although the interactions between rice straw decomposition and priming effects on native SOC are not yet understood, there was considerable variation between Karst and red soils. Soil calcium was a positive factor in maintaining SOC stability. MBC from rice straws was stable in terra fusca and rendzina, whereas it was active in the red soil. The Karst soils (terra fusca and rendzina) used in this study benefited SOC accumulation., (Copyright © 2011 Society of Chemical Industry.)

Soil microorganisms play vital roles in recovering and maintaining the health of ecosystems, particularly in fragile Karst ecosystems that are easily degraded after cultivation. We investigated the composition and diversity of soil bacterial communities, based on RFLP and 16S rDNA sequencing, in a cropland, a naturally revegetated land with former cultivation disturbance and a primeval forest in the subtropical Karst of southwest China. Our results illustrated that Proteobacteria accounted for 44.8% of the 600 tested clones, making it the most dominant phylum observed. This phylum was followed by Acidobacteria and Planctomycetes for the three Karst soils analyzed. Compared with the primeval forest soil, the proportions of Proteobacteria were decreased by 30.2 and 37.9%, while Acidobacteria increased by 93.9 and 87.9%, and the Shannon-Wiener diversity indices and the physicochemical parameters declined in the cropland and the revegetated land, respectively. Among the three soils, the proportion of dominant bacterial phyla and the diversity indices in the revegetated land were similar to the cropland, implying the bacterial community in the cropland was relatively stable, and the after-effects of cultivation were difficult to eliminate. However, similar distributions of the four Proteobacteria subphyla were observed between the revegetated land and the primeval forest soil. Furthermore, the proportion of Rhizobiales belonging to α-Proteobacteria was sharply decreased with cultivation compared to the primeval forest soil, while a small cluster of Rhizobiales recurred with vegetation recovery. These results indicated that although the subphyla of the dominant bacterial phylum had some positive responses to 20 years of vegetation recovery, it is a slow process. Our results suggest that priority should be given to conserve the primeval forest and inoculation of functional microorganisms on the basis of vegetation recovery may be more effective for the restoration of Karst ecosystems after cultivation.