Search

Longitudinal studies of gut microbiota following specific interventions are vital for understanding how they influence host health. However, robust longitudinal sampling of gut microbiota is a major challenge, which can be addressed using in vitro fermentors hosting complex microbial communities. Here, by employing 16S rRNA gene amplicon sequencing, we investigated the adaptation and succession of human fecal microbial communities in an automated multistage fermentor. We performed two independent experiments using different human donor fecal samples, one configured with two units of three colon compartments each studied for 22 days and another with one unit of two colon compartments studied for 31 days. The fermentor maintained a trend of increasing microbial alpha diversity along colon compartments. Within each experiment, microbial compositions followed compartment-specific trajectories and reached independent stable configurations. While compositions were highly similar between replicate units, they were clearly separated between different experiments, showing that they maintained the individuality of fecal inoculum rather than converging on a fermentor-specific composition. While some fecal amplicon sequence variants (ASVs) were undetected in the fermentor, many ASVs undetected in the fecal samples flourished in vitro . These bloomer ASVs accounted for significant proportions of the population and included prominent health-associated microbes such as Bacteroides fragilis and Akkermansia muciniphila. Turnover in community compositions is likely explained by feed composition and pH, suggesting that these communities can be easily modulated. Our results suggest that in vitro fermentors are promising tools to study complex microbial communities harboring important members of human gut microbiota. IMPORTANCE In vitro fermentors that can host complex gut microbial communities are promising tools to investigate the dynamics of human gut microbiota. In this work, using an automated in vitro gut fermentor consisting of different colon compartments, we investigated the adaptation dynamics of two different human fecal microbial communities over 22 and 31 days. By observing the temporal trends of different community members, we found that many dominant members of the fecal microbiota failed to maintain their dominance in vitro , and some of the low-abundance microbes undetected in the fecal microbiota successfully grew in the in vitro communities. Microbiome compositional changes and blooming could largely be explained by feed composition and pH, suggesting that these communities can be modulated to desired compositions via optimizing culture conditions. Thus, our results open up the possibility of modulating in vitro microbial communities to predefined compositions by optimizing feed composition and culture conditions.

A principled approach to recover communities in social networks is to find a clustering of the network nodes into modules (i.e groups of nodes) for which the modularity over the network is maximal. This guarantees partitioning the network nodes into sparsely connected groups of densely connected nodes. A popular extension of modularity has been proposed in the literature so it applies to multi-layer networks, that is, networks that model different types/aspects of interactions among a set of actors. In this extension, a new parameter, the coupling strength ω, has been introduced to couple different copies (i.e nodes) of the same actor with specific weights across different layers. This allows two nodes that refer to the same actor to reward the modularity score with an amount proportional to ω when they appear in the same community. While this extension seems to provide an effective tool to detect communities in multi-layer networks, it is not always clear what kind of communities maximising the generalised modularity can identify in multi-layer networks and whether these communities are inclusive to all possible community structures possible to exist in multi-layer networks. In addition, it has not been thoroughly investigated yet how to interpret ω in real-world scenarios, and whether a proper tuning of ω, if exists, is enough to guarantee an accurate recoverability for different types of multi-layer community structures. In this article, we report the different ways used in the literature to tune ω. We analyse different community structures that can be recovered by maximising the generalised modularity in relation to ω. We propose different models for multi-layer communities in multiplex and time-dependent networks and test if they are recoverable by modularity-maximization community detection methods under any assignment of ω. Our main finding is that only few simple models of multi-layer communities in multiplex and time-dependent networks are recoverable by modularity maximisation methods while more complex models are not accurately recoverable under any assignment of ω.

We propose a way to model topic-based implicit interactions among Twitter users. Our model relies on grouping Twitter hashtags, in a given context, into themes/topics and then using the multiplex network model to construct a thematic multiplex where each layer corresponds to a topic/theme, and users within a layer are connected if and only if they used the same hashtag. We show, by testing our model on a real-world Twitter dataset, that applying multiplex community detection on the thematic multiplex can reveal new types of communities that were not observed before using the traditional ways of modeling Twitter interactions., (Copyright © 2019 Hanteer and Rossi.)