1. Development and characterization of a Chinese hamster ovary cell-specific oligonucleotide microarray.
- Author
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Melville M, Doolan P, Mounts W, Barron N, Hann L, Leonard M, Clynes M, and Charlebois T
- Subjects
- Animals, CHO Cells, Cricetinae, Cricetulus, Female, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling methods, Oligonucleotide Array Sequence Analysis methods
- Abstract
The Chinese hamster ovary (CHO) cell line is one of the most widely used mammalian cell lines for biopharmaceutical production. We have developed and characterized a gene expression microarray (WyeHamster2a) specific for CHO cells that has enabled the study of ~3,500 sequences. Analysis of multiple sets of replicate scans showed that data derived from the WyeHamster2a array is highly reproducible confirming it as a robust tool for profiling. Twelve gene sequences were selected for follow-up RT-qPCR to confirm the accuracy and precision of the microarray results. In all but the most subtle gene expression differences, the microarray proved to be a reliable measure of differential gene expression. Finally, we were able to quantify the difference between using a bona fide CHO-specific microarray for profiling CHO cells versus an alternate, commercially available, rodent microarray such as a mouse or rat-specific format., (© Springer Science+Business Media B.V. 2011)
- Published
- 2011
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