14 results on '"Grisanzio, Chiara"'
Search Results
2. Cell kinetic studies fail to identify sequentially proliferating progenitors as the major source of epithelial renewal in the adult murine prostate.
- Author
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Pignon JC, Grisanzio C, Carvo I, Werner L, Regan M, Wilson EL, and Signoretti S
- Subjects
- Animals, Epithelium metabolism, Male, Mice, Prostate metabolism, Stem Cells metabolism, Cell Proliferation physiology, Prostate cytology, Stem Cells cytology
- Abstract
There is evidence that stem cells and their progeny play a role in the development of the prostate. Although stem cells are also considered to give rise to differentiated progeny in the adult prostate epithelium ex vivo, the cohort of adult prostate stem cells in vivo as well as the mechanisms by which the adult prostate epithelium is maintained and regenerated remain highly controversial. We have attempted to resolve this conundrum by performing in vivo tracing of serially replicating cells after the sequential administration of two thymidine analogues to mice. Our results show that, during normal prostate homeostasis, sequentially proliferating cells are detected at a rate that is consistent with a stochastic process. These findings indicate that in vivo, under steady-state conditions, most adult prostate epithelial cells do not represent the progeny of a small number of specialized progenitors that generate sequentially replicating transit-amplifying (TA) cells but are formed by stochastic cell division. Similarly, no rapidly cycling TA cells were detected during regeneration following one cycle of androgen-mediated involution/regeneration of the prostate epithelium. These findings greatly enhance our understanding of the mechanisms regulating prostate epithelial cell renewal and may have significant implications in defining the cell of origin of proliferative prostatic diseases.
- Published
- 2015
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3. p63-expressing cells are the stem cells of developing prostate, bladder, and colorectal epithelia.
- Author
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Pignon JC, Grisanzio C, Geng Y, Song J, Shivdasani RA, and Signoretti S
- Subjects
- Animals, Carcinoma, Squamous Cell metabolism, Cell Line, Tumor, Cell Lineage, Colorectal Neoplasms genetics, Female, Gene Knock-In Techniques, Genotype, Male, Metaplasia metabolism, Mice, Mice, Transgenic, Neoplastic Stem Cells metabolism, Phosphoproteins genetics, Promoter Regions, Genetic, Prostatic Neoplasms genetics, Protein Structure, Tertiary, Stem Cells cytology, Trans-Activators genetics, Urinary Bladder Neoplasms genetics, Colorectal Neoplasms metabolism, Gene Expression Regulation, Neoplastic, Neoplastic Stem Cells cytology, Phosphoproteins metabolism, Prostatic Neoplasms metabolism, Trans-Activators metabolism, Urinary Bladder Neoplasms metabolism
- Abstract
The tumor protein p63 (p63), and more specifically the NH2-terminal truncated (ΔN) p63 isoform, is a marker of basal epithelial cells and is required for normal development of several epithelial tissues, including the bladder and prostate glands. Although p63-expressing cells are proposed to be the stem cells of the developing prostate epithelium and bladder urothelium, cell lineages in these endoderm-derived epithelia remain highly controversial, and rigorous lineage tracing studies are warranted. Here, we generated knock-in mice expressing Cre recombinase (Cre) under the control of the endogenous ΔNp63 promoter. Heterozygote ΔNp63(+/Cre) mice were phenotypically normal and fertile. Cre-mediated recombination in ΔNp63(+/Cre);ROSA26(EYFP) reporter mice faithfully recapitulated the pattern of ΔNp63 expression and were useful for genetic lineage tracing of ΔNp63-expressing cells of the caudal endoderm in vivo. We found that ΔNp63-positive cells of the urogenital sinus generated all epithelial lineages of the prostate and bladder, indicating that these cells represent the stem/progenitor cells of those epithelia during development. We also observed ΔNp63 expression in caudal gut endoderm and the contribution of ΔNp63-positive cells to the stem/progenitor compartment of adult colorectal epithelium. Because p63 is a master regulator of stratified epithelial development, this finding provides a unique developmental insight into the cell of origin of squamous cell metaplasia and squamous cell carcinoma of the colon.
- Published
- 2013
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4. Genetic and functional analyses implicate the NUDT11, HNF1B, and SLC22A3 genes in prostate cancer pathogenesis.
- Author
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Grisanzio C, Werner L, Takeda D, Awoyemi BC, Pomerantz MM, Yamada H, Sooriakumaran P, Robinson BD, Leung R, Schinzel AC, Mills I, Ross-Adams H, Neal DE, Kido M, Yamamoto T, Petrozziello G, Stack EC, Lis R, Kantoff PW, Loda M, Sartor O, Egawa S, Tewari AK, Hahn WC, and Freedman ML
- Subjects
- Alleles, Gene Expression Profiling, Genome-Wide Association Study, Humans, Male, Models, Genetic, Phenotype, Polymorphism, Genetic, Polymorphism, Single Nucleotide, Prostatic Neoplasms genetics, Quantitative Trait Loci, Risk, Gene Expression Regulation, Neoplastic, Hepatocyte Nuclear Factor 1-beta biosynthesis, Organic Cation Transport Proteins biosynthesis, Prostatic Neoplasms metabolism, Pyrophosphatases biosynthesis
- Abstract
One of the central goals of human genetics is to discover the genes and pathways driving human traits. To date, most of the common risk alleles discovered through genome-wide association studies (GWAS) map to nonprotein-coding regions. Because of our relatively poorer understanding of this part of the genome, the functional consequences of trait-associated variants pose a considerable challenge. To identify the genes through which risk loci act, we hypothesized that the risk variants are regulatory elements. For each of 12 known risk polymorphisms, we evaluated the correlation between risk allele status and transcript abundance for all annotated protein-coding transcripts within a 1-Mb interval. A total of 103 transcripts were evaluated in 662 prostate tissue samples [normal (n = 407) and tumor (n = 255)] from 483 individuals [European Americans (n = 233), Japanese (n = 127), and African Americans (n = 123)]. In a pooled analysis, 4 of the 12 risk variants were strongly associated with five transcripts (NUDT11, MSMB, NCOA4, SLC22A3, and HNF1B) in histologically normal tissue (P ≤ 0.001). Although associations were also observed in tumor tissue, they tended to be more attenuated. Previously, we showed that MSMB and NCOA4 participate in prostate cancer pathogenesis. Suppressing the expression of NUDT11, SLC22A3, and HNF1B influences cellular phenotypes associated with tumor-related properties in prostate cancer cells. Taken together, the data suggest that these transcripts contribute to prostate cancer pathogenesis.
- Published
- 2012
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5. Orthotopic xenografts of RCC retain histological, immunophenotypic and genetic features of tumours in patients.
- Author
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Grisanzio C, Seeley A, Chang M, Collins M, Di Napoli A, Cheng SC, Percy A, Beroukhim R, and Signoretti S
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- Adolescent, Aged, Aged, 80 and over, Animals, Carcinoma, Renal Cell immunology, Female, Humans, Immunophenotyping, Kidney Neoplasms immunology, Male, Mice, Mice, Inbred NOD, Mice, SCID, Middle Aged, Transplantation, Heterologous, Carcinoma, Renal Cell genetics, Carcinoma, Renal Cell pathology, Disease Models, Animal, Kidney Neoplasms genetics, Kidney Neoplasms pathology
- Abstract
Renal cell carcinoma (RCC) is an aggressive malignancy with limited responsiveness to existing treatments. In vivo models of human cancer, including RCC, are critical for developing more effective therapies. Unfortunately, current RCC models do not accurately represent relevant properties of the human disease. The goal of this study was to develop clinically relevant animal models of RCC for preclinical investigations. We transplanted intact human tumour tissue fragments orthotopically in immunodeficient mice. The xenografts were validated by comparing the morphological, phenotypic and genetic characteristics of the kidney tumour tissues before and after implantation. Twenty kidney tumours were transplanted into mice. Successful tumour growth was detected in 19 cases (95%). The histopathological and immunophenotypic features of the xenografts and those of the original tumours largely overlapped in all cases. Evaluation of genetic alterations in a subset of 10 cases demonstrated that the grafts largely retained the genetic features of the pre-implantation RCC tissues. Indeed, primary tumours and corresponding grafts displayed identical VHL mutations. Moreover, an identical pattern of DNA copy amplification or loss was observed in 6/10 cases (60%). In summary, orthotopic engrafting of RCC tissue fragments can be successfully used to generate animal models that closely resemble RCC in patients. These models will be invaluable for in vivo preclinical drug testing and for deeper understanding of kidney carcinogenesis. The raw data of the SNP array analysis has been submitted to the GEO database (Accession No. GSE29062)., (Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.)
- Published
- 2011
- Full Text
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6. Loss of the retinoblastoma binding protein 2 (RBP2) histone demethylase suppresses tumorigenesis in mice lacking Rb1 or Men1.
- Author
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Lin W, Cao J, Liu J, Beshiri ML, Fujiwara Y, Francis J, Cherniack AD, Geisen C, Blair LP, Zou MR, Shen X, Kawamori D, Liu Z, Grisanzio C, Watanabe H, Minamishima YA, Zhang Q, Kulkarni RN, Signoretti S, Rodig SJ, Bronson RT, Orkin SH, Tuck DP, Benevolenskaya EV, Meyerson M, Kaelin WG Jr, and Yan Q
- Subjects
- Animals, Enzyme Inhibitors therapeutic use, Epigenomics, Histone Demethylases, Histones metabolism, Methylation, Mice, Mice, Knockout, Neoplasms enzymology, Neoplasms etiology, Retinol-Binding Proteins, Cellular antagonists & inhibitors, Survival Rate, Neoplasms prevention & control, Proto-Oncogene Proteins deficiency, Retinoblastoma Protein deficiency, Retinol-Binding Proteins, Cellular deficiency
- Abstract
Aberrations in epigenetic processes, such as histone methylation, can cause cancer. Retinoblastoma binding protein 2 (RBP2; also called JARID1A or KDM5A) can demethylate tri- and dimethylated lysine 4 in histone H3, which are epigenetic marks for transcriptionally active chromatin, whereas the multiple endocrine neoplasia type 1 (MEN1) tumor suppressor promotes H3K4 methylation. Previous studies suggested that inhibition of RBP2 contributed to tumor suppression by the retinoblastoma protein (pRB). Here, we show that genetic ablation of Rbp2 decreases tumor formation and prolongs survival in Rb1(+/-) mice and Men1-defective mice. These studies link RBP2 histone demethylase activity to tumorigenesis and nominate RBP2 as a potential target for cancer therapy.
- Published
- 2011
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7. Carbonic anhydrase IX expression in renal neoplasms: correlation with tumor type and grade.
- Author
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Genega EM, Ghebremichael M, Najarian R, Fu Y, Wang Y, Argani P, Grisanzio C, and Signoretti S
- Subjects
- Adenoma, Oxyphilic secondary, Biomarkers, Tumor metabolism, Carbonic Anhydrase IX, Carcinoma, Papillary secondary, Carcinoma, Renal Cell secondary, Humans, Immunoenzyme Techniques, Kidney Neoplasms pathology, Adenoma, Oxyphilic enzymology, Antigens, Neoplasm metabolism, Carbonic Anhydrases metabolism, Carcinoma, Papillary enzymology, Carcinoma, Renal Cell enzymology, Kidney Neoplasms enzymology
- Abstract
Carbonic anhydrase IX (CAIX), a hypoxia-induced protein, is expressed in some renal tumors. We evaluated its immunohistochemical expression in 317 primary and 42 metastatic renal neoplasms (186 clear cell, 52 papillary, 35 chromophobe, 47 unclassified, and 15 Xp11.2 translocation renal cell carcinomas [RCCs]; 26 oncocytomas; 2 metanephric adenomas; 1 urothelial carcinoma; 1 mixed epithelial and stromal tumor; and 1 angiomyolipoma); 7 neoplasms were unknown as to whether they were primary or metastatic. We also correlated expression with tumor type and grade. Variable staining was seen in clear cell, papillary, unclassified, and Xp11.2 translocation carcinomas. One chromophobe carcinoma had focal expression. No staining was seen with other tumors. An association was found between high expression and clear cell vs non-clear cell carcinomas with all cases (P < .01) and primary (P < .01) cases. An association between CAIX expression and grade (P < .01) in primary clear cell carcinomas was found. CAIX expression is more common in clear cell RCC than other renal tumor types and is associated with grade.
- Published
- 2010
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8. Chromosome 8q24-Associated Cancers and MYC.
- Author
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Grisanzio C and Freedman ML
- Abstract
Genome-wide association studies (GWAS) have successfully identified common polymorphisms that are strongly associated with many traits, including cancer. A gene desert located on chromosome 8q24 is associated with multiple cancer types. One of the closest genes is the MYC proto-oncogene. Investigations are now turning toward a mechanistic understanding of these (and other) risk loci. Recent studies demonstrate that the 8q24 loci are enhancers and that they physically interact with MYC. A still unresolved issue is the absence of a consistent association between genotype status at the risk loci and steady-state MYC expression levels in adult human tissues. Clarifying the function of the 8q24 variants and their link to MYC regulation by further in vivo and in vitro functional studies will allow a deeper understanding of the mechanisms underlying human cancer development.
- Published
- 2010
- Full Text
- View/download PDF
9. 8q24 prostate, breast, and colon cancer risk loci show tissue-specific long-range interaction with MYC.
- Author
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Ahmadiyeh N, Pomerantz MM, Grisanzio C, Herman P, Jia L, Almendro V, He HH, Brown M, Liu XS, Davis M, Caswell JL, Beckwith CA, Hills A, Macconaill L, Coetzee GA, Regan MM, and Freedman ML
- Subjects
- Cell Line, Tumor, Genetic Loci, Genome, Human, Humans, Organ Specificity, Proto-Oncogene Mas, Risk Factors, Breast Neoplasms genetics, Chromosomes, Human, Pair 8, Colonic Neoplasms genetics, Epigenesis, Genetic, Gene Expression Regulation, Neoplastic, Proto-Oncogene Proteins c-myc genetics
- Abstract
The 8q24 gene desert contains risk loci for multiple epithelial cancers, including colon, breast, and prostate. Recent evidence suggests these risk loci contain enhancers. In this study, data are presented showing that each risk locus bears epigenetic marks consistent with enhancer elements and forms a long-range chromatin loop with the MYC proto-oncogene located several hundred kilobases telomeric and that these interactions are tissue-specific. We therefore propose that the 8q24 risk loci operate through a common mechanism-as tissue-specific enhancers of MYC.
- Published
- 2010
- Full Text
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10. Fatty acid synthase: a metabolic enzyme and candidate oncogene in prostate cancer.
- Author
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Migita T, Ruiz S, Fornari A, Fiorentino M, Priolo C, Zadra G, Inazuka F, Grisanzio C, Palescandolo E, Shin E, Fiore C, Xie W, Kung AL, Febbo PG, Subramanian A, Mucci L, Ma J, Signoretti S, Stampfer M, Hahn WC, Finn S, and Loda M
- Subjects
- Animals, Bromodeoxyuridine metabolism, Cell Line, Tumor, Disease Models, Animal, Fatty Acid Synthase, Type I metabolism, Flow Cytometry, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Gene Silencing, Humans, Immunoblotting, Immunohistochemistry, Male, Mice, Mice, Transgenic, Orchiectomy, RNA, Small Interfering metabolism, Transplantation, Heterologous, Up-Regulation, Adenocarcinoma enzymology, Adenocarcinoma genetics, Apoptosis genetics, Fatty Acid Synthase, Type I genetics, Oncogenes, Prostatic Neoplasms enzymology, Prostatic Neoplasms genetics
- Abstract
Background: Overexpression of the fatty acid synthase (FASN) gene has been implicated in prostate carcinogenesis. We sought to directly assess the oncogenic potential of FASN., Methods: We used immortalized human prostate epithelial cells (iPrECs), androgen receptor-overexpressing iPrECs (AR-iPrEC), and human prostate adenocarcinoma LNCaP cells that stably overexpressed FASN for cell proliferation assays, soft agar assays, and tests of tumor formation in immunodeficient mice. Transgenic mice expressing FASN in the prostate were generated to assess the effects of FASN on prostate histology. Apoptosis was evaluated by Hoechst 33342 staining and by fluorescence-activated cell sorting in iPrEC-FASN cells treated with stimulators of the intrinsic and extrinsic pathways of apoptosis (ie, camptothecin and anti-Fas antibody, respectively) or with a small interfering RNA (siRNA) targeting FASN. FASN expression was compared with the apoptotic index assessed by the terminal deoxynucleotidyltransferase-mediated UTP end-labeling method in 745 human prostate cancer samples by using the least squares means procedure. All statistical tests were two-sided., Results: Forced expression of FASN in iPrECs, AR-iPrECs, and LNCaP cells increased cell proliferation and soft agar growth. iPrECs that expressed both FASN and androgen receptor (AR) formed invasive adenocarcinomas in immunodeficient mice (12 of 14 mice injected formed tumors vs 0 of 14 mice injected with AR-iPrEC expressing empty vector (P < .001, Fisher exact test); however, iPrECs that expressed only FASN did not. Transgenic expression of FASN in mice resulted in prostate intraepithelial neoplasia, the incidence of which increased from 10% in 8- to 16-week-old mice to 44% in mice aged 7 months or more (P = .0028, Fisher exact test), but not in invasive tumors. In LNCaP cells, siRNA-mediated silencing of FASN resulted in apoptosis. FASN overexpression protected iPrECs from apoptosis induced by camptothecin but did not protect iPrECs from Fas receptor-induced apoptosis. In human prostate cancer specimens, FASN expression was inversely associated with the apoptotic rate (mean percentage of apoptotic cells, lowest vs highest quartile of FASN expression: 2.76 vs 1.34, difference = 1.41, 95% confidence interval = 0.45 to 2.39, Ptrend = .0046)., Conclusions: These observations suggest that FASN can act as a prostate cancer oncogene in the presence of AR and that FASN exerts its oncogenic effect by inhibiting the intrinsic pathway of apoptosis.
- Published
- 2009
- Full Text
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11. A prostatic intraepithelial neoplasia-dependent p27 Kip1 checkpoint induces senescence and inhibits cell proliferation and cancer progression.
- Author
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Majumder PK, Grisanzio C, O'Connell F, Barry M, Brito JM, Xu Q, Guney I, Berger R, Herman P, Bikoff R, Fedele G, Baek WK, Wang S, Ellwood-Yen K, Wu H, Sawyers CL, Signoretti S, Hahn WC, Loda M, and Sellers WR
- Subjects
- Alleles, Animals, Animals, Genetically Modified, Biomarkers metabolism, Cell Adhesion, Cell Communication, Cell Line, Cell Polarity, Cell Proliferation, Disease Progression, Epithelial Cells pathology, Humans, Male, Mice, Mutation genetics, Phenotype, Protein Kinases metabolism, Proto-Oncogene Proteins c-akt metabolism, Rats, TOR Serine-Threonine Kinases, Cellular Senescence, Cyclin-Dependent Kinase Inhibitor p27 metabolism, Prostatic Intraepithelial Neoplasia metabolism, Prostatic Intraepithelial Neoplasia pathology, Prostatic Neoplasms metabolism, Prostatic Neoplasms pathology
- Abstract
Transgenic expression of activated AKT1 in the murine prostate induces prostatic intraepithelial neoplasia (PIN) that does not progress to invasive prostate cancer (CaP). In luminal epithelial cells of Akt-driven PIN, we show the concomitant induction of p27(Kip1) and senescence. Genetic ablation of p27(Kip1) led to downregulation of senescence markers and progression to cancer. In humans, p27(Kip1) and senescence markers were elevated in PIN not associated with CaP but were decreased or absent, respectively, in cancer-associated PIN and in CaP. Importantly, p27(Kip1) upregulation in mouse and human in situ lesions did not depend upon mTOR or Akt activation but was instead specifically associated with alterations in cell polarity, architecture, and adhesion molecules. These data suggest that a p27(Kip1)-driven checkpoint limits progression of PIN to CaP.
- Published
- 2008
- Full Text
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12. p63 in prostate biology and pathology.
- Author
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Grisanzio C and Signoretti S
- Subjects
- Animals, Cell Transformation, Neoplastic metabolism, Cell Transformation, Neoplastic pathology, Epithelium metabolism, Epithelium pathology, Humans, Male, Prostate pathology, Prostatic Neoplasms diagnosis, Prostatic Neoplasms pathology, Stem Cells cytology, Transcription Factors, Biomarkers, Tumor metabolism, Cell Differentiation, Prostate metabolism, Prostatic Neoplasms metabolism, Stem Cells pathology, Trans-Activators metabolism, Tumor Suppressor Proteins metabolism
- Abstract
The identification of stem cells and differentiation programs regulating the development and maintenance of the normal prostate epithelium is essential for the identification of the cell type(s) and molecular alterations involved in the development and propagation of prostate cancer (CaP). The p53-homologue p63 is highly expressed in normal prostate basal cells and is a clinically useful biomarker for the diagnosis of CaP. Importantly, p63 has been shown to play a critical role in prostate development. Recent experimental evidence also suggests that this gene is essential for normal stem cell function in the prostate as well as other epithelial organs. Future studies aimed at better defining the role of p63 in the renewal of the adult prostate epithelium are likely to shed new light on the mechanisms involved in prostate carcinogenesis., (2007 Wiley-Liss, Inc.)
- Published
- 2008
- Full Text
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13. VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400.
- Author
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Young AP, Schlisio S, Minamishima YA, Zhang Q, Li L, Grisanzio C, Signoretti S, and Kaelin WG Jr
- Subjects
- Animals, Cells, Cultured, Fibroblasts metabolism, Inositol 1,4,5-Trisphosphate Receptors, Mice, Mice, Transgenic, Mutation, Oxygen metabolism, Phenotype, Retroviridae metabolism, Von Hippel-Lindau Tumor Suppressor Protein metabolism, Calcium Channels metabolism, Cellular Senescence, Gene Expression Regulation, Hypoxia-Inducible Factor 1 metabolism, Membrane Glycoproteins metabolism, Receptors, Cytoplasmic and Nuclear metabolism, Retinoblastoma Protein metabolism, Von Hippel-Lindau Tumor Suppressor Protein physiology
- Abstract
Germline von Hippel-Lindau tumour suppressor gene (VHL) mutations cause renal cell carcinomas, haemangioblastomas and phaeochromocytomas in humans. Mutations in VHL also occur in sporadic renal cell carcinomas. The protein encoded by VHL, VHL, is part of the ubiquitin ligase that downregulates the heterodimeric transcription factor Hif under well-oxygenated conditions. Here we show that acute VHL inactivation causes a senescent-like phenotype in vitro and in vivo. This phenotype was independent of p53 and Hif but dependent on the retinoblastoma protein (Rb) and the SWI2/SNF2 chromatin remodeller p400. Rb activation occurred through a decrease in Skp2 messenger RNA, which resulted in the upregulation of p27 in a Hif-independent fashion. Our results suggest that senescence induced by VHL inactivation is a tumour-suppressive mechanism that must be overcome to develop VHL-associated neoplasias.
- Published
- 2008
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14. p63 regulates commitment to the prostate cell lineage.
- Author
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Signoretti S, Pires MM, Lindauer M, Horner JW, Grisanzio C, Dhar S, Majumder P, McKeon F, Kantoff PW, Sellers WR, and Loda M
- Subjects
- Animals, Epithelial Cells metabolism, Epithelial Cells physiology, Female, Histocytochemistry, Male, Mice, Mice, Knockout, Prostate embryology, Stem Cells physiology, Transplantation Chimera embryology, Urothelium embryology, beta-Galactosidase, Cell Differentiation physiology, Cell Lineage physiology, Phosphoproteins physiology, Prostate cytology, Trans-Activators physiology
- Abstract
Molecular mechanisms underlying prostate and urothelial development remain unclear. This situation presents major limitations in identifying the cell type(s) and molecular events involved in the development of prostate and bladder cancer. It has been shown that mice lacking the basal cell marker p63 present several epithelial defects, including epidermis and prostate buds agenesis and urothelial abnormalities. Here, we use the p63-/- mouse as a tool to define cell lineages in the prostate epithelium and urothelium. By complementing p63-/- blastocysts with p63+/+ beta-galactosidase (beta-gal)-positive ES cells, we show that secretory cells of the prostate originate from p63-positive basal progenitor cells. Importantly, our urogenital sinus transplantation studies demonstrate that p63 prevents intestinal differentiation of the urogenital sinus endoderm and is therefore required to maintain commitment to the prostate cell lineage. Finally, in contrast with the prostate findings, analysis of the urothelium from rescued p63-/- chimeras shows that umbrella (superficial) cells can develop and be maintained independently from p63-positive basal and intermediate cells.
- Published
- 2005
- Full Text
- View/download PDF
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