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Although blood remains a gold standard diagnostic fluid for most health exams, it involves an unpleasant and relatively invasive sampling procedure (finger pricking or venous draw). Saliva contains many relevant and useful biomarkers for diagnostic purposes, and its collection, in contrast, is noninvasive and can be obtained with minimal effort. Current saliva analyses are, however, achieved using chromatography or lateral flow assays, which, despite their high accuracy and sensitivity, can demand expensive laboratory-based instruments operated by trained personnel or offer only semiquantitative results. In response, we investigated electrochemical aptamer-based (E-AB) biosensors, a reagentless sensing platform, to allow for continuous and real-time measurements directly in undiluted, unstimulated human whole saliva. As a proof-of-concept study, we developed E-AB biosensors capable of detecting low-molecular-weight analytes (glucose and adenosine monophosphate (AMP)). To our knowledge, we report the first E-AB sensor for glucose, an approach that is inherently independent of its chemical reactivity in contrast to home glucometers. For these three sensors, we evaluated their figures of merits, stability, and reusability over short- and long-term exposure directly in saliva. In doing so, we found that E-AB sensors allow rapid and convenient molecular measurements in whole saliva with unprecedented sensitivities in the pico- to nanomolar regime and could be regenerated and reused up to 7 days when washed and stored in phosphate-buffered saline at room temperature. We envision that salivary molecular measurements using E-AB sensors are a promising alternative to invasive techniques and can be used for improved point-of-care clinical diagnosis and at-home measurements.

Interest in sensors and their applications is rapidly evolving, mainly driven by the huge demand of technologies whose ultimate purpose is to improve and enhance health and safety. Different electromagnetic technologies have been recently used and achieved good performances. Despite the plethora of literature, limitations are still present: limited response control, narrow bandwidth, and large dimensions. MetaSurfaces, artificial 2D materials with peculiar electromagnetic properties, can help to overcome such issues. In this paper, a generic tool to model, design, and manufacture MetaSurface sensors is developed. First, their properties are evaluated in terms of impedance and constitutive parameters. Then, they are linked to the structure physical dimensions. Finally, the proposed method is applied to realize devices for advanced sensing and medical diagnostic applications: glucose measurements, cancer stage detection, water content recognition, and blood oxygen level analysis. The proposed method paves a new way to realize sensors and control their properties at will. Most importantly, it has great potential to be used for many other practical applications, beyond sensing and diagnostics., Competing Interests: The author declares no conflict of interest.

A modeling and design approach is proposed for nanoparticle-based electromagnetic devices. First, the structure properties were analytically studied using Maxwell's equations. The method provides us a robust link between nanoparticles electromagnetic response (amplitude and phase) and their geometrical characteristics (shape, geometry, and dimensions). Secondly, new designs based on "metamaterial" concept are proposed, demonstrating great performances in terms of wide-angle range functionality and multi/wide behavior, compared to conventional devices working at the same frequencies. The approach offers potential applications to build-up new advanced platforms for sensing and medical diagnostics. Therefore, in the final part of the article, some practical examples are reported such as cancer detection, water content measurements, chemical analysis, glucose concentration measurements and blood diseases monitoring., Competing Interests: The authors declare no conflict of interest.