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Acoustophoresis is an effective technique for particle manipulation. Acoustic radiation force scales with particle volume, enabling size separation. Yet, isolating sub-micron particles remains a challenge due to the acoustic streaming effect (ASE). While some studies confirmed the focusing ability of ASE, others reported continuous stirring effects. To investigate the parameters that influence ASE-induced particle motion in a microchannel, this study examined the effect of microchannel height and particle size. We employed standing surface acoustic wave (SSAW) to manipulate polystyrene particles suspended in the water-filled microchannel. The results show that ASE can direct particles as small as 0.31 µm in diameter to the centre of the streaming vortices, and increasing the channel height enhances the focusing effect. Smaller particles circulate in the streaming vortices continuously, with no movement towards the centres. We also discovered that when the channel height is at least 0.75 the fluid wavelength, particles transitioning from acoustic radiation-dominated to ASE-dominated share the same equilibrium position, which differs from the pressure nodes and the vortices' centres. The spatial distance between particles in different categories can lead to particle separation. Therefore, ASE is a potential alternative mechanism for sub-micron particle sorting when the channel height is accurately adjusted., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Head orientations directly determine movement directions of exhaled pathogen-laden droplets, while there is a lack of research about the effect of the infected person's head orientations on respiratory disease transmission during close contact. This work experimentally investigated the effect of different head orientations of an infected person (IP) on the bioaerosol deposition on a healthy person (HP) during close contact. Also, the effectiveness of PV flow in reducing bioaerosol deposition on the HP under the IP's different head orientations was investigated. Bacteriophage T3 was employed to represent viruses inside the cough-generated aerosols. The bioaerosol depositions on different locations of the HP's upper body (chest, shoulder, and neck) and face (chin, mucous membranes, cheek, and forehead) were characterized by a cultivation method. Results showed that the IP's different head orientations resulted in significantly different deposition density on the HP. PV flow could reduce the bioaerosol deposition remarkably for most cases investigated. The effectiveness of PV flow in reducing deposition on the HP was significantly affected by the IP's head orientations. Findings suggest that changing head orientations can be a control measure to reduce the bioaerosol deposition. Personalized ventilation can be a potential method to reduce the bioaerosol deposition on the HP., (© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Respiratory bioaerosol deposition in public transport cabins is critical for risk analysis and control of contact transmission. In this work, we built a two-row four-seat setup and an air duct system to simulate a cabin environment. A thermal manikin on the rear left-hand seat was taken as the infected passenger (IP) and "coughed" three times through a cough generator. The deposited viruses and droplets on nearby seats were measured by a cultivation method and microscope, respectively. The effects of seat backrest and overhead gasper jet were studied. Results showed that the number of deposited virus on the front seat was one order of magnitude higher than that on other seats which only contained droplets smaller than 10 µm in diameter. When the backrest was 15 cm higher than the cough, the deposited number of viruses was reduced to 5% of that with the backrest at the same height with the cough. The gasper jet above the IP with a velocity of 1.5 m/s can reduce the deposited viruses to 4% of that with gasper off. It indicates that both the gasper jet and backrest can work as mitigation measures to block the cough jet and protect the nearby passengers., (© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Particle resuspension has been recognized as a secondary source of indoor air pollution by many field studies. However, some laboratory studies showed that the air velocities or force fields required to resuspend aerosol particles are very high that rarely occurred in indoor environments. In fact, the surfaces used in these studies were treated to ensure cleanliness, but in reality, dusty surfaces are ubiquitous in our daily life. This work aims to investigate the effect of dust on a surface on resuspension of a coarse particle (polyethylene) by a centrifugal method. Dusty surfaces with different loadings were made by gravitational settling of Arizona test dust on a clean poly(methyl methacrylate) substrate inside a deposition chamber. The resuspension of dust particles was first investigated, and it was found that dust particles were resuspended by two stages with different rates of resuspension. For the resuspension of the particles on the dusty surface, the remaining fraction of the polyethylene particles decreased with increasing force field and dust loading. Dust could greatly reduce the adhesion of the particles from one to two orders of magnitude depending on loadings. This gives an explanation to the discrepancy between the field and the laboratory studies., (© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Comparative transcriptome analysis was used to determine the differentially expressed genes in Escherichia coli during aerosolization from liquid suspension. Isogenic mutant studies were then used to examine the potential part played by some of these genes in bacterial survival in the air. Bioaerosols were sampled after 3 min of nebulization, which aerosolized the bacteria from the liquid suspension to an aerosol chamber (A0), and after further 30 min of airborne suspension in the chamber (A30). Bacteria at A0 showed 65 differentially expressed genes (30 downregulated and 35 upregulated) as compared to the original bacteria in the nebulizer. Droplet evaporation models predicted a drop in temperature in the bioaerosols, which coincides with the change in the expression of cold shock protein genes-cspB and cspG in the bacteria. The most notable group of differentially expressed genes was sorbitol transport and metabolism genes (srlABDEMR). Other genes associated with osmotic stress, nutrient limitation, DNA damage, and other stresses were differentially expressed in the bacteria at A0. After further airborne suspension, one gene (ypfM, which encodes a hypothetical protein with unknown function) was downregulated in the bacteria at A30 as compared to those at A0. Finally, isogenic mutants with either the dps or srlA gene deleted (both genes were upregulated at A0) had lower survival than the parental strain, which is a sign of their potential ability to protect the bacteria in the air.

Background: Airborne transmission of respiratory infectious disease in indoor environment (e.g. airplane cabin, conference room, hospital, isolated room and inpatient ward) may cause outbreaks of infectious diseases, which may lead to many infection cases and significantly influences on the public health. This issue has received more and more attentions from academics. This work investigates the influence of human movement on the airborne transmission of respiratory infectious diseases in an airplane cabin by using an accurate human model in numerical simulation and comparing the influences of different human movement behaviors on disease transmission., Methods: The Eulerian-Lagrangian approach is adopted to simulate the dispersion and deposition of the expiratory aerosols. The dose-response model is used to assess the infection risks of the occupants. The likelihood analysis is performed as a hypothesis test on the input parameters and different human movement pattern assumptions. An in-flight SARS outbreak case is used for investigation. A moving person with different moving speeds is simulated to represent the movement behaviors. A digital human model was used to represent the detailed profile of the occupants, which was obtained by scanning a real thermal manikin using the 3D laser scanning system., Results: The analysis results indicate that human movement can strengthen the downward transport of the aerosols, significantly reduce the overall deposition and removal rate of the suspended aerosols and increase the average infection risk in the cabin. The likelihood estimation result shows that the risk assessment results better fit the outcome of the outbreak case when the movements of the seated passengers are considered. The intake fraction of the moving person is significantly higher than most of the seated passengers., Conclusions: The infection risk distribution in the airplane cabin highly depends on the movement behaviors of the passengers and the index patient. The walking activities of the crew members and the seated passengers can significantly increase their personal infection risks. Taking the influence of the movement of the seated passengers and the index patient into consideration is necessary and important. For future studies, investigations on the behaviors characteristics of the passengers during flight will be useful and helpful for infection control.